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The map label for this gene is ispH

Identifier: 119356252

GI number: 119356252

Start: 457509

End: 458495

Strand: Reverse

Name: ispH

Synonym: Cpha266_0414

Alternate gene names: 119356252

Gene position: 458495-457509 (Counterclockwise)

Preceding gene: 119356253

Following gene: 119356251

Centisome position: 14.63

GC content: 48.02

Gene sequence:

>987_bases
GTGAAAATCAATCTTGACAGAACATCATCCGGTTTCTGCATCGGCGTACAGGGCACCATCCATGTTGCCGAAGAAAAACT
CAATAATCCGGATAAATTATTTTCGCTCGGTGATGTTGTGCATAACGAAGCTGAAGTCAACAGACTTGAACATCTCGGCC
TGACAACCATCGATGAGTCAGGATTTCACGAACTGAAAAATGCGCAACTCCTCATCAGGGCTCATGGCGAACCCCCGTCA
ACATACCGTATTGCAGAGAAAAACAACCTTGTCATTACGGATACGACCTGCCCTGTAGTCTCAAGACTGCAACGCACCGC
AAGACTGCTCCATGAGCTTGGCTACCAGATCATCATTTACGGGAAGCCCTCCCATCCCGAAGTAATCGGAATCAATGGCC
ATTGCAAAAACAGTGCGGTCATCATCAAACACCCCGACCTTTCCGATCCTTCAGAGACCGGTCAAATTGATCTGAAAAGA
AAAACAGCACTCATCTCACAAACAACCATGGATGTTCCGGGGTTTTATGAGCTGAAAGCAAACATCCGTAACCTCTTTGC
TGCCGATTCTGAAGAAATACGTAACGAGAACGACCATCTCTGGATGGCTATCCGCGACATTGATCTCACGGCCTCATTGA
CCGGCATTCGCGACATGCCGCGTTACGTTTACAAAGACACCATATGTCGGCAGGTTTCGAGCAGAAACCAGATGCTGCAT
GATTTTGCGATTGCAAACAGTTGCGTCATTTTTGTCGCCGGCAAAAAAAGTTCAAACGGACAGGTTCTTTTCGGCATATG
CAAAGCCGCAAATCCCTGCACCTATTTCATTGAGGATATTGATGAAATAGACGATGCATGGCTGACCGGGAGTGATGGCC
GGACGGTTGAAAGTGTGGGTATCTGCGGAGCCACCTCTACGCCAATGTGGCTGCTTGAAAAAGTTGCGGCACACCTCCAG
AGACGATATAATCACGATCCCGCATGA

Upstream 100 bases:

>100_bases
TTTGTTTCCGGCAATTGTTGCCTTTTGACTCTGGACTTCTTACCTTTGACATTTCAGGGCCCGGGATCCTTCGGCCTGTA
AACATGACGATACTTGCAAC

Downstream 100 bases:

>100_bases
CATCCTCTCATGACACCGATACAAACTTCATCATGAACGCTCTCACCCTTACCATTAACGGGCTGAAAACAACAGTTCAA
CCCGGAACTTCCATTCTTGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNNPDKLFSLGDVVHNEAEVNRLEHLGLTTIDESGFHELKNAQLLIRAHGEPPS
TYRIAEKNNLVITDTTCPVVSRLQRTARLLHELGYQIIIYGKPSHPEVIGINGHCKNSAVIIKHPDLSDPSETGQIDLKR
KTALISQTTMDVPGFYELKANIRNLFAADSEEIRNENDHLWMAIRDIDLTASLTGIRDMPRYVYKDTICRQVSSRNQMLH
DFAIANSCVIFVAGKKSSNGQVLFGICKAANPCTYFIEDIDEIDDAWLTGSDGRTVESVGICGATSTPMWLLEKVAAHLQ
RRYNHDPA

Sequences:

>Translated_328_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNNPDKLFSLGDVVHNEAEVNRLEHLGLTTIDESGFHELKNAQLLIRAHGEPPS
TYRIAEKNNLVITDTTCPVVSRLQRTARLLHELGYQIIIYGKPSHPEVIGINGHCKNSAVIIKHPDLSDPSETGQIDLKR
KTALISQTTMDVPGFYELKANIRNLFAADSEEIRNENDHLWMAIRDIDLTASLTGIRDMPRYVYKDTICRQVSSRNQMLH
DFAIANSCVIFVAGKKSSNGQVLFGICKAANPCTYFIEDIDEIDDAWLTGSDGRTVESVGICGATSTPMWLLEKVAAHLQ
RRYNHDPA
>Mature_328_residues
MKINLDRTSSGFCIGVQGTIHVAEEKLNNPDKLFSLGDVVHNEAEVNRLEHLGLTTIDESGFHELKNAQLLIRAHGEPPS
TYRIAEKNNLVITDTTCPVVSRLQRTARLLHELGYQIIIYGKPSHPEVIGINGHCKNSAVIIKHPDLSDPSETGQIDLKR
KTALISQTTMDVPGFYELKANIRNLFAADSEEIRNENDHLWMAIRDIDLTASLTGIRDMPRYVYKDTICRQVSSRNQMLH
DFAIANSCVIFVAGKKSSNGQVLFGICKAANPCTYFIEDIDEIDDAWLTGSDGRTVESVGICGATSTPMWLLEKVAAHLQ
RRYNHDPA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=320, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHLPD (A1BDJ5)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910896.1
- STRING:   A1BDJ5
- GeneID:   4570968
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0414
- NMPDR:   fig|290317.7.peg.425
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   MDVPGFY
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36522; Mature: 36522

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLNNPDKLFSLGDVVHNEAEVNRLEHLGLTTIDES
CEEECCCCCCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEECCC
GFHELKNAQLLIRAHGEPPSTYRIAEKNNLVITDTTCPVVSRLQRTARLLHELGYQIIIY
CHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
GKPSHPEVIGINGHCKNSAVIIKHPDLSDPSETGQIDLKRKTALISQTTMDVPGFYELKA
ECCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
NIRNLFAADSEEIRNENDHLWMAIRDIDLTASLTGIRDMPRYVYKDTICRQVSSRNQMLH
HHHHHHCCCHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DFAIANSCVIFVAGKKSSNGQVLFGICKAANPCTYFIEDIDEIDDAWLTGSDGRTVESVG
HHHHCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEE
ICGATSTPMWLLEKVAAHLQRRYNHDPA
ECCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKINLDRTSSGFCIGVQGTIHVAEEKLNNPDKLFSLGDVVHNEAEVNRLEHLGLTTIDES
CEEECCCCCCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEECCC
GFHELKNAQLLIRAHGEPPSTYRIAEKNNLVITDTTCPVVSRLQRTARLLHELGYQIIIY
CHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEE
GKPSHPEVIGINGHCKNSAVIIKHPDLSDPSETGQIDLKRKTALISQTTMDVPGFYELKA
ECCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
NIRNLFAADSEEIRNENDHLWMAIRDIDLTASLTGIRDMPRYVYKDTICRQVSSRNQMLH
HHHHHHCCCHHHHCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DFAIANSCVIFVAGKKSSNGQVLFGICKAANPCTYFIEDIDEIDDAWLTGSDGRTVESVG
HHHHCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEE
ICGATSTPMWLLEKVAAHLQRRYNHDPA
ECCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA