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The map label for this gene is aegA [H]

Identifier: 119356251

GI number: 119356251

Start: 455497

End: 457476

Strand: Reverse

Name: aegA [H]

Synonym: Cpha266_0413

Alternate gene names: 119356251

Gene position: 457476-455497 (Counterclockwise)

Preceding gene: 119356252

Following gene: 119356248

Centisome position: 14.6

GC content: 53.23

Gene sequence:

>1980_bases
ATGAACGCTCTCACCCTTACCATTAACGGGCTGAAAACAACAGTTCAACCCGGAACTTCCATTCTTGATGCAGCAGCATC
ATGCGGCATCACCATACCCACCCTCTGTTTTCAGCAATCCCTCGACGTTCAGGGCGCATGCTGGATCTGTATCGTGGAAA
TCAAAGGGAAAAATCGCTTTGTTCCGGCATGCAGCACAAAAGTCTCCGCGGGTATGGTTATAGAAACTGAAACCGAAGAG
CTTCATATGATGCGACGCCGAACCCTCGAACGCCTCATTGAACAACATTGCGGAGACTGTCTCGGCCAATGCGAACTGGC
CTGCCCGGCAGGATGCAACATCCCGGGGTTCATTTCGGAAATTGCAGCTCACCATGATGACGAAGCAATTGCAATCATCA
AAAACACTATCCCTCTTCCCGGTATTCTCGGAAGGGTATGTCCTGCTCCGTGCGAAGATGCCTGCCGCCGACACGGTGTT
GATGACCCGGTATCAATCTGCGCCCTGAAACGCTATGCCGCCGACAGGGATATGGAAGCAAGCGAACGGTATATTCCGGC
TATTGCGGAAAAATCCGGAAAACATGTAGCAGTCATAGGGGCCGGGCCTGCTGGTCTGACTGCCGCCTACTATCTTCTCG
CTCTTGGCCATGAGGTCACCATTTATGATGAACATCCTGCCGCGGGCGGCATGATGCGTTACGGCATCCCGCGCTTCAGG
CTTCCCGAATCGGTTATTGACAAGGATGTGGAGCCTCTTGAACAGATGGGCGCAACATTCCGGTTCAACACCCGTTTCGG
TAAAGATATCTCGACAGCATCGCTTCAGAGTGCGTTTGATGCGATATTTCTTGCCGTCGGGGCACAGCAGGCCGCGGCAA
TGCATATCCCCGGCGAACATGAAACAGGAGTGCTCAGCGGCATTGAGTTCCTGCGCCAGGCCAACGAAGGCGTTGCAGAG
CATCCAGGCGCGAATGTGGTTGTCGTTGGCGGAGGCAATACCGCCGTTGATGCAGCAAGAACGGCCCTGCGACTTGGAGC
GACAACGGTTACTATTCTCTATCGCCGGACAAAAGAAGAGATGCCGGCAAACCAGCAGGAGATCAATGAGGCGATCGCCG
AAGGCATTACCCTGCATGTTCTTGCCCTTCCAGTCTCCATGCGGCGCATGAACGGATTCACCGAAATCACCGCTATCGCC
ATGCGTCAAGGAGAGCCGGATGAAAGCGGACGAAAAAAACCTGTACCGATCAACGGGAGCGAATTCACCATAGCAGCAAA
TACGGTCATCTCTGCAACAGGTCAACAGGTAGACTGTTCAATTGCTATGGCTGCCGGGATCGGAACAAGCCCTGAAGGCA
CCTTCGACGTTAACCAGGCGACGCTGCAATCGAACATTGCCGGTATTTTCAGCGCCGGTGACTGCGTAACAGGACCTGAC
CTGGCGATCACTGCCGTCAGCGAGGGCAGACGTGCCGCTCATTCGATCCATCTTTTTCTGCAGGGCAAGCCTGTCGTTGG
CAAACCTCAACAGTTCAACTCCTCCTACGGCCCCAGAGACCAGGCTCCCGAAGCATTCTACAGCAAAGCAAACCCTTTTG
AAAAGATTGCCTTGCCCGAAATTTCGCTCCTGGAGCGATCTGGCACGTTCAGGGAAGTTGTTCTCGGACTCTCTCCTGAA
GATGCCGCTCAGGAAGCCCTGCGCTGCCTTCAATGTCGATGCAACGCCATTGATAACTGTCGACTTCGTGAACTTGCGGC
TCTCTACCTTCCTTCAGGTAGCTGCAAACTCGAAGAAAAACTCGATTTTGAGATCATTTCAGGAACCGATATTCGCATCG
AGCGGGAAAAGTGCGTTGATTGCGGCATCTGTGTCAGAACGCTTGAACAGTATGGAACGGAACATGAACCCGACTATCAA
AAGCTATCCACATCCTGCCCGACAGGGGCGCTTAGCGAAGCAATGCTGAATAAAAAATAA

Upstream 100 bases:

>100_bases
CGCCAATGTGGCTGCTTGAAAAAGTTGCGGCACACCTCCAGAGACGATATAATCACGATCCCGCATGACATCCTCTCATG
ACACCGATACAAACTTCATC

Downstream 100 bases:

>100_bases
CCGCTCATATCAGGCACCCCTCTCATCGGGATTTCAACAGATCAAAAAACGACTTGATCATGGTTGACACGTGACTGAAG
GAAGCTGCAATCGTTTCCTG

Product: putative NADPH-dependent glutamate synthase small subunit

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MNALTLTINGLKTTVQPGTSILDAAASCGITIPTLCFQQSLDVQGACWICIVEIKGKNRFVPACSTKVSAGMVIETETEE
LHMMRRRTLERLIEQHCGDCLGQCELACPAGCNIPGFISEIAAHHDDEAIAIIKNTIPLPGILGRVCPAPCEDACRRHGV
DDPVSICALKRYAADRDMEASERYIPAIAEKSGKHVAVIGAGPAGLTAAYYLLALGHEVTIYDEHPAAGGMMRYGIPRFR
LPESVIDKDVEPLEQMGATFRFNTRFGKDISTASLQSAFDAIFLAVGAQQAAAMHIPGEHETGVLSGIEFLRQANEGVAE
HPGANVVVVGGGNTAVDAARTALRLGATTVTILYRRTKEEMPANQQEINEAIAEGITLHVLALPVSMRRMNGFTEITAIA
MRQGEPDESGRKKPVPINGSEFTIAANTVISATGQQVDCSIAMAAGIGTSPEGTFDVNQATLQSNIAGIFSAGDCVTGPD
LAITAVSEGRRAAHSIHLFLQGKPVVGKPQQFNSSYGPRDQAPEAFYSKANPFEKIALPEISLLERSGTFREVVLGLSPE
DAAQEALRCLQCRCNAIDNCRLRELAALYLPSGSCKLEEKLDFEIISGTDIRIEREKCVDCGICVRTLEQYGTEHEPDYQ
KLSTSCPTGALSEAMLNKK

Sequences:

>Translated_659_residues
MNALTLTINGLKTTVQPGTSILDAAASCGITIPTLCFQQSLDVQGACWICIVEIKGKNRFVPACSTKVSAGMVIETETEE
LHMMRRRTLERLIEQHCGDCLGQCELACPAGCNIPGFISEIAAHHDDEAIAIIKNTIPLPGILGRVCPAPCEDACRRHGV
DDPVSICALKRYAADRDMEASERYIPAIAEKSGKHVAVIGAGPAGLTAAYYLLALGHEVTIYDEHPAAGGMMRYGIPRFR
LPESVIDKDVEPLEQMGATFRFNTRFGKDISTASLQSAFDAIFLAVGAQQAAAMHIPGEHETGVLSGIEFLRQANEGVAE
HPGANVVVVGGGNTAVDAARTALRLGATTVTILYRRTKEEMPANQQEINEAIAEGITLHVLALPVSMRRMNGFTEITAIA
MRQGEPDESGRKKPVPINGSEFTIAANTVISATGQQVDCSIAMAAGIGTSPEGTFDVNQATLQSNIAGIFSAGDCVTGPD
LAITAVSEGRRAAHSIHLFLQGKPVVGKPQQFNSSYGPRDQAPEAFYSKANPFEKIALPEISLLERSGTFREVVLGLSPE
DAAQEALRCLQCRCNAIDNCRLRELAALYLPSGSCKLEEKLDFEIISGTDIRIEREKCVDCGICVRTLEQYGTEHEPDYQ
KLSTSCPTGALSEAMLNKK
>Mature_659_residues
MNALTLTINGLKTTVQPGTSILDAAASCGITIPTLCFQQSLDVQGACWICIVEIKGKNRFVPACSTKVSAGMVIETETEE
LHMMRRRTLERLIEQHCGDCLGQCELACPAGCNIPGFISEIAAHHDDEAIAIIKNTIPLPGILGRVCPAPCEDACRRHGV
DDPVSICALKRYAADRDMEASERYIPAIAEKSGKHVAVIGAGPAGLTAAYYLLALGHEVTIYDEHPAAGGMMRYGIPRFR
LPESVIDKDVEPLEQMGATFRFNTRFGKDISTASLQSAFDAIFLAVGAQQAAAMHIPGEHETGVLSGIEFLRQANEGVAE
HPGANVVVVGGGNTAVDAARTALRLGATTVTILYRRTKEEMPANQQEINEAIAEGITLHVLALPVSMRRMNGFTEITAIA
MRQGEPDESGRKKPVPINGSEFTIAANTVISATGQQVDCSIAMAAGIGTSPEGTFDVNQATLQSNIAGIFSAGDCVTGPD
LAITAVSEGRRAAHSIHLFLQGKPVVGKPQQFNSSYGPRDQAPEAFYSKANPFEKIALPEISLLERSGTFREVVLGLSPE
DAAQEALRCLQCRCNAIDNCRLRELAALYLPSGSCKLEEKLDFEIISGTDIRIEREKCVDCGICVRTLEQYGTEHEPDYQ
KLSTSCPTGALSEAMLNKK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=449, Percent_Identity=26.5033407572383, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI111118983, Length=158, Percent_Identity=32.2784810126582, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=32.2784810126582, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI33519475, Length=110, Percent_Identity=33.6363636363636, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=421, Percent_Identity=37.7672209026128, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789606, Length=422, Percent_Identity=33.8862559241706, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI87082180, Length=420, Percent_Identity=34.2857142857143, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789243, Length=606, Percent_Identity=29.2079207920792, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788468, Length=406, Percent_Identity=31.2807881773399, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=30.5122494432071, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71984108, Length=427, Percent_Identity=25.9953161592506, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17543860, Length=159, Percent_Identity=35.2201257861635, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565758, Length=104, Percent_Identity=31.7307692307692, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566231, Length=104, Percent_Identity=31.7307692307692, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193209088, Length=104, Percent_Identity=31.7307692307692, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=439, Percent_Identity=28.0182232346241, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24665547, Length=427, Percent_Identity=27.4004683840749, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24665543, Length=427, Percent_Identity=27.4004683840749, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28574881, Length=427, Percent_Identity=27.4004683840749, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24665539, Length=427, Percent_Identity=27.4004683840749, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=28.8018433179723, Blast_Score=136, Evalue=6e-32,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=28.8018433179723, Blast_Score=136, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640559, Length=104, Percent_Identity=33.6538461538462, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640557, Length=104, Percent_Identity=33.6538461538462, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137178, Length=159, Percent_Identity=30.188679245283, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 70658; Mature: 70658

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALTLTINGLKTTVQPGTSILDAAASCGITIPTLCFQQSLDVQGACWICIVEIKGKNRF
CCEEEEEEECCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCEE
VPACSTKVSAGMVIETETEELHMMRRRTLERLIEQHCGDCLGQCELACPAGCNIPGFISE
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
IAAHHDDEAIAIIKNTIPLPGILGRVCPAPCEDACRRHGVDDPVSICALKRYAADRDMEA
HHHCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SERYIPAIAEKSGKHVAVIGAGPAGLTAAYYLLALGHEVTIYDEHPAAGGMMRYGIPRFR
HHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCC
LPESVIDKDVEPLEQMGATFRFNTRFGKDISTASLQSAFDAIFLAVGAQQAAAMHIPGEH
CCHHHHCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCC
ETGVLSGIEFLRQANEGVAEHPGANVVVVGGGNTAVDAARTALRLGATTVTILYRRTKEE
CCHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHH
MPANQQEINEAIAEGITLHVLALPVSMRRMNGFTEITAIAMRQGEPDESGRKKPVPINGS
CCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFTIAANTVISATGQQVDCSIAMAAGIGTSPEGTFDVNQATLQSNIAGIFSAGDCVTGPD
EEEEEECEEEECCCCEEEEEEEEEECCCCCCCCCEECHHHHHHHHHHEEEECCCCCCCCC
LAITAVSEGRRAAHSIHLFLQGKPVVGKPQQFNSSYGPRDQAPEAFYSKANPFEKIALPE
EEEEEECCCCCCEEEEEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHCCCC
ISLLERSGTFREVVLGLSPEDAAQEALRCLQCRCNAIDNCRLRELAALYLPSGSCKLEEK
HHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
LDFEIISGTDIRIEREKCVDCGICVRTLEQYGTEHEPDYQKLSTSCPTGALSEAMLNKK
CCEEEECCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNALTLTINGLKTTVQPGTSILDAAASCGITIPTLCFQQSLDVQGACWICIVEIKGKNRF
CCEEEEEEECCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCEE
VPACSTKVSAGMVIETETEELHMMRRRTLERLIEQHCGDCLGQCELACPAGCNIPGFISE
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
IAAHHDDEAIAIIKNTIPLPGILGRVCPAPCEDACRRHGVDDPVSICALKRYAADRDMEA
HHHCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SERYIPAIAEKSGKHVAVIGAGPAGLTAAYYLLALGHEVTIYDEHPAAGGMMRYGIPRFR
HHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCC
LPESVIDKDVEPLEQMGATFRFNTRFGKDISTASLQSAFDAIFLAVGAQQAAAMHIPGEH
CCHHHHCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCC
ETGVLSGIEFLRQANEGVAEHPGANVVVVGGGNTAVDAARTALRLGATTVTILYRRTKEE
CCHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHHHH
MPANQQEINEAIAEGITLHVLALPVSMRRMNGFTEITAIAMRQGEPDESGRKKPVPINGS
CCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFTIAANTVISATGQQVDCSIAMAAGIGTSPEGTFDVNQATLQSNIAGIFSAGDCVTGPD
EEEEEECEEEECCCCEEEEEEEEEECCCCCCCCCEECHHHHHHHHHHEEEECCCCCCCCC
LAITAVSEGRRAAHSIHLFLQGKPVVGKPQQFNSSYGPRDQAPEAFYSKANPFEKIALPE
EEEEEECCCCCCEEEEEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHCCCC
ISLLERSGTFREVVLGLSPEDAAQEALRCLQCRCNAIDNCRLRELAALYLPSGSCKLEEK
HHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
LDFEIISGTDIRIEREKCVDCGICVRTLEQYGTEHEPDYQKLSTSCPTGALSEAMLNKK
CCEEEECCCCEEEEHHHHCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]