Click here to switch to the map view.

The map label for this gene is pcm

Identifier: 119356178

GI number: 119356178

Start: 369907

End: 370557

Strand: Direct

Name: pcm

Synonym: Cpha266_0339

Alternate gene names: 119356178

Gene position: 369907-370557 (Clockwise)

Preceding gene: 119356176

Following gene: 119356179

Centisome position: 11.8

GC content: 49.77

Gene sequence:

>651_bases
ATGGATAGTAATTCCAGGCTCATGACGTTCAGGCGCAGAGAGATGGTTGAAAAGCTGAAGTTGAGCGGTATTGCAGATAA
CCGGGTTCTTGCTGCGTTTTTAAGTGTTGAACGTCACCGTTTTTTTGACAAGGAAAGTCAGGAGTATGCGTATGCGGATG
GAGCATATCCCATAGGGTATGGCCAGACCATTTCGCAGCCCTATACAGTAGCCTGTATGACATCGTTGCTTGTGGAACGA
TCGCCGTCAGGTAAAGTTCTCGAAATAGGGACTGGATCGGGGTATCAGGCAGCGATTCTTTACGCTCTCGGGTACAGGGT
TTATACTATTGAGCGGGTTGTGGAACTCTCTGAAAAAGCCGAAAAGCTCTTCAGTGCTCTGGGGTTCTCTATAATGTGCC
GATCAGGAGACGGAACGCTCGGCTGGCCCGAGGAGGCGCCGTTTGACGGTATTATGGTTACAGCTGGAGCGCCTCATTGT
CCCGAAACGCTCTTGCATCAGCTTGCTCTCGACGGGAGCCTTGTTGTGCCGATTGGAGCGCATGGTTTGCAGAAAATGAC
GGTTTTTCAAAGAAAAAAAGACCGTTTCGAAAAGGAAGTATTCAGTGATTTTGCTTTCGTGCCGCTGATAGGCAGGGAAG
GGTGGGGTTGA

Upstream 100 bases:

>100_bases
AATATTAGCGGTGCTGAAAATAAAACGCTGATGTTATCCTGTTCAATGATCGGAAGATTTTCATGCAGGAAAAAGATAAT
TGTCTTAACGGTACTTTGCT

Downstream 100 bases:

>100_bases
TTTTTTTAAACATCTTAATGAAGGGATGATTTATGGCTGTAATGACCAGCTTAAGGGATAAGACTCATATCATTCTTTAT
GCGCTTCTGGCTGCGTTTCT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDSNSRLMTFRRREMVEKLKLSGIADNRVLAAFLSVERHRFFDKESQEYAYADGAYPIGYGQTISQPYTVACMTSLLVER
SPSGKVLEIGTGSGYQAAILYALGYRVYTIERVVELSEKAEKLFSALGFSIMCRSGDGTLGWPEEAPFDGIMVTAGAPHC
PETLLHQLALDGSLVVPIGAHGLQKMTVFQRKKDRFEKEVFSDFAFVPLIGREGWG

Sequences:

>Translated_216_residues
MDSNSRLMTFRRREMVEKLKLSGIADNRVLAAFLSVERHRFFDKESQEYAYADGAYPIGYGQTISQPYTVACMTSLLVER
SPSGKVLEIGTGSGYQAAILYALGYRVYTIERVVELSEKAEKLFSALGFSIMCRSGDGTLGWPEEAPFDGIMVTAGAPHC
PETLLHQLALDGSLVVPIGAHGLQKMTVFQRKKDRFEKEVFSDFAFVPLIGREGWG
>Mature_216_residues
MDSNSRLMTFRRREMVEKLKLSGIADNRVLAAFLSVERHRFFDKESQEYAYADGAYPIGYGQTISQPYTVACMTSLLVER
SPSGKVLEIGTGSGYQAAILYALGYRVYTIERVVELSEKAEKLFSALGFSIMCRSGDGTLGWPEEAPFDGIMVTAGAPHC
PETLLHQLALDGSLVVPIGAHGLQKMTVFQRKKDRFEKEVFSDFAFVPLIGREGWG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=213, Percent_Identity=28.169014084507, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1789100, Length=208, Percent_Identity=41.3461538461538, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71983477, Length=212, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207222, Length=211, Percent_Identity=27.0142180094787, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17981723, Length=222, Percent_Identity=30.6306306306306, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_CHLPD (A1BDC1)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910822.1
- ProteinModelPortal:   A1BDC1
- SMR:   A1BDC1
- STRING:   A1BDC1
- GeneID:   4569511
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0339
- NMPDR:   fig|290317.7.peg.345
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   CLSK637215
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23977; Mature: 23977

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNSRLMTFRRREMVEKLKLSGIADNRVLAAFLSVERHRFFDKESQEYAYADGAYPIGY
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
GQTISQPYTVACMTSLLVERSPSGKVLEIGTGSGYQAAILYALGYRVYTIERVVELSEKA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLFSALGFSIMCRSGDGTLGWPEEAPFDGIMVTAGAPHCPETLLHQLALDGSLVVPIGA
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECC
HGLQKMTVFQRKKDRFEKEVFSDFAFVPLIGREGWG
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MDSNSRLMTFRRREMVEKLKLSGIADNRVLAAFLSVERHRFFDKESQEYAYADGAYPIGY
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
GQTISQPYTVACMTSLLVERSPSGKVLEIGTGSGYQAAILYALGYRVYTIERVVELSEKA
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLFSALGFSIMCRSGDGTLGWPEEAPFDGIMVTAGAPHCPETLLHQLALDGSLVVPIGA
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECC
HGLQKMTVFQRKKDRFEKEVFSDFAFVPLIGREGWG
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA