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The map label for this gene is hycE [C]

Identifier: 118581925

GI number: 118581925

Start: 3897506

End: 3897970

Strand: Reverse

Name: hycE [C]

Synonym: Ppro_3525

Alternate gene names: 118581925

Gene position: 3897970-3897506 (Counterclockwise)

Preceding gene: 118581926

Following gene: 118581924

Centisome position: 97.25

GC content: 61.29

Gene sequence:

>465_bases
ATGACACGCGAAACAACCATTGCCGAGGAGCTGGCCAGACGTTTTCCCGGGCTCAAGGAGAGTATCCGGGTCCAGCGGGA
ACGACGCATCTTTGTGGACACCCCTGCGGAGACCCTCAACAGCCTGATCGATTTCGCCTACAGCGAACTTGGGTTCATCT
CCTTGGTATCCCTGGTGGGAACCGACGAGGGGGAGACTCTGGGGGTCATGTACGTGCTGGCCGCCGATGACGGGATCCTG
CTGACCGTCAGGCGCTATCAGCCGAAGACCGATCCGGTGATCGCCACCATGACCGGGAAATTCCCCAACGCGGAGTACTA
CGAGAAAGAACTGGTGGACCTGCTCGGCTTCAGAATCGAGGGTCTGCCCCCCGGCCCCCGCTACCCGCTGCCGGATGACT
GGCCCGAGGGCCAGTACCCCCTGCGCAAGGAGTGGAAGACGGAAATGCTTGACAGGAAGGAGTGA

Upstream 100 bases:

>100_bases
CCCCGAGGCGATCATCGACGGCGTGGTCAAGTTGCTGTCAACTCTGAAAGCAGACTGATATACTAAACAACAGTGGTATC
ACCAGGAAGGCAGGCTGGAC

Downstream 100 bases:

>100_bases
TTGCCATGTCACCGGTCACCATCCCCATCGGCCCGCAGCATCCCGCGCTGAAGGAGCCGGCCAACTTCTCCATTACCGTT
ACCGGCGAGAAGATTGTCAC

Product: NADH dehydrogenase (ubiquinone), 30 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: Ni Fe-Hydrogenase III Large Subunit; Hydrogenase Related Subunit; Ech Protein; NADH-Ubiquinone Oxidoreductase; Membrane Bound Hydrogenase MbhK Subunit

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTRETTIAEELARRFPGLKESIRVQRERRIFVDTPAETLNSLIDFAYSELGFISLVSLVGTDEGETLGVMYVLAADDGIL
LTVRRYQPKTDPVIATMTGKFPNAEYYEKELVDLLGFRIEGLPPGPRYPLPDDWPEGQYPLRKEWKTEMLDRKE

Sequences:

>Translated_154_residues
MTRETTIAEELARRFPGLKESIRVQRERRIFVDTPAETLNSLIDFAYSELGFISLVSLVGTDEGETLGVMYVLAADDGIL
LTVRRYQPKTDPVIATMTGKFPNAEYYEKELVDLLGFRIEGLPPGPRYPLPDDWPEGQYPLRKEWKTEMLDRKE
>Mature_153_residues
TRETTIAEELARRFPGLKESIRVQRERRIFVDTPAETLNSLIDFAYSELGFISLVSLVGTDEGETLGVMYVLAADDGILL
TVRRYQPKTDPVIATMTGKFPNAEYYEKELVDLLGFRIEGLPPGPRYPLPDDWPEGQYPLRKEWKTEMLDRKE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3262

COG function: function code C; Ni,Fe-hydrogenase III component G

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789076, Length=140, Percent_Identity=33.5714285714286, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 17671; Mature: 17540

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRETTIAEELARRFPGLKESIRVQRERRIFVDTPAETLNSLIDFAYSELGFISLVSLVG
CCCCHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
TDEGETLGVMYVLAADDGILLTVRRYQPKTDPVIATMTGKFPNAEYYEKELVDLLGFRIE
CCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEC
GLPPGPRYPLPDDWPEGQYPLRKEWKTEMLDRKE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRETTIAEELARRFPGLKESIRVQRERRIFVDTPAETLNSLIDFAYSELGFISLVSLVG
CCCHHHHHHHHHHCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
TDEGETLGVMYVLAADDGILLTVRRYQPKTDPVIATMTGKFPNAEYYEKELVDLLGFRIE
CCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEC
GLPPGPRYPLPDDWPEGQYPLRKEWKTEMLDRKE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA