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The map label for this gene is hycG [C]

Identifier: 118581926

GI number: 118581926

Start: 3898013

End: 3898447

Strand: Reverse

Name: hycG [C]

Synonym: Ppro_3526

Alternate gene names: 118581926

Gene position: 3898447-3898013 (Counterclockwise)

Preceding gene: 118581927

Following gene: 118581925

Centisome position: 97.27

GC content: 61.38

Gene sequence:

>435_bases
ATGAAAGCACTGGACAAACTGGTGACATGGGCGCGGATCAAATCCCCCTGGGTGATCCACTTCAATACCGGCGCCTGCAA
CGGTTGCGACATCGAAATTGTCGATTCGCTGACCCCCCGCTTCGATCTGGAGCGCTTCGGAATCCAGCTCAAGGGAACTC
CCCGGCATGCCGATGTGCTGCTCTGTTCCGGCCCGGTAACTAGGCAGACCAAGGACCGCCTGCTGCGCATCTACGAGCAG
ATGGCGGACCCTAAATTTGTCATTGCGGTGGGAACCTGCGCCTGCTCCGCCGGCGTCTTCCAGGGGTGCTACAACGTGCT
GGGGGGGACAGATACGGTGCTGCCCGTCTCGGCCTACATCCCCGGCTGCGCCGCCCGCCCCGAGGCGATCATCGACGGCG
TGGTCAAGTTGCTGTCAACTCTGAAAGCAGACTGA

Upstream 100 bases:

>100_bases
ACGGTCCCCTCCGGTTCCCTCCCGGATTCGCTGTTTGCCGCACTGTTCACCATCTCCGCGGCAACGGGGCTGGCGGCACT
CTTCAGGAAGGATGCCTGAC

Downstream 100 bases:

>100_bases
TATACTAAACAACAGTGGTATCACCAGGAAGGCAGGCTGGACATGACACGCGAAACAACCATTGCCGAGGAGCTGGCCAG
ACGTTTTCCCGGGCTCAAGG

Product: NADH-quinone oxidoreductase subunit B

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MKALDKLVTWARIKSPWVIHFNTGACNGCDIEIVDSLTPRFDLERFGIQLKGTPRHADVLLCSGPVTRQTKDRLLRIYEQ
MADPKFVIAVGTCACSAGVFQGCYNVLGGTDTVLPVSAYIPGCAARPEAIIDGVVKLLSTLKAD

Sequences:

>Translated_144_residues
MKALDKLVTWARIKSPWVIHFNTGACNGCDIEIVDSLTPRFDLERFGIQLKGTPRHADVLLCSGPVTRQTKDRLLRIYEQ
MADPKFVIAVGTCACSAGVFQGCYNVLGGTDTVLPVSAYIPGCAARPEAIIDGVVKLLSTLKAD
>Mature_144_residues
MKALDKLVTWARIKSPWVIHFNTGACNGCDIEIVDSLTPRFDLERFGIQLKGTPRHADVLLCSGPVTRQTKDRLLRIYEQ
MADPKFVIAVGTCACSAGVFQGCYNVLGGTDTVLPVSAYIPGCAARPEAIIDGVVKLLSTLKAD

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=141, Percent_Identity=36.8794326241135, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1789074, Length=129, Percent_Identity=45.7364341085271, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788834, Length=129, Percent_Identity=43.4108527131783, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1788624, Length=142, Percent_Identity=36.6197183098592, Blast_Score=103, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=139, Percent_Identity=38.1294964028777, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=36.6906474820144, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=36.6906474820144, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24651058, Length=139, Percent_Identity=36.6906474820144, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 15595; Mature: 15595

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALDKLVTWARIKSPWVIHFNTGACNGCDIEIVDSLTPRFDLERFGIQLKGTPRHADVL
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCHHHCCEEECCCCCCCCEE
LCSGPVTRQTKDRLLRIYEQMADPKFVIAVGTCACSAGVFQGCYNVLGGTDTVLPVSAYI
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEHHHHC
PGCAARPEAIIDGVVKLLSTLKAD
CCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKALDKLVTWARIKSPWVIHFNTGACNGCDIEIVDSLTPRFDLERFGIQLKGTPRHADVL
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCHHHCCEEECCCCCCCCEE
LCSGPVTRQTKDRLLRIYEQMADPKFVIAVGTCACSAGVFQGCYNVLGGTDTVLPVSAYI
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEHHHHC
PGCAARPEAIIDGVVKLLSTLKAD
CCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]