Click here to switch to the map view.

The map label for this gene is cooH [H]

Identifier: 118581924

GI number: 118581924

Start: 3896271

End: 3897500

Strand: Reverse

Name: cooH [H]

Synonym: Ppro_3524

Alternate gene names: 118581924

Gene position: 3897500-3896271 (Counterclockwise)

Preceding gene: 118581925

Following gene: 118581923

Centisome position: 97.24

GC content: 63.25

Gene sequence:

>1230_bases
ATGTCACCGGTCACCATCCCCATCGGCCCGCAGCATCCCGCGCTGAAGGAGCCGGCCAACTTCTCCATTACCGTTACCGG
CGAGAAGATTGTCACCACCAGCATGCGTCTCGGCTACAACCACCGGGGGATCGAGAAGGCGTGCGAAGAACGCTCGTACC
TGCAGTGCCTCTATCTGCTGGAGCGGATCTGCGGAATCTGCTCCCACACCCACTCCACGGCCTACATCCAGGCGGTTGAG
GAGATCGCCGGCCTGACCATTCCGCCACGGGCGAACTACATCCGCTCACTGGTGGGAGAGCTGGAGCGGATCCACAGTCA
CCTGCTCTGGCTGGGGGTGGCCGGGCACGAGATCGGTTTCGACACCCTGTTGATGTACAGCTGGCGCGACCGCGAACTGG
TCATGGACATACTGGCGGAACTGGGGGGCAACCGGGTCAACTACGGCATCAACACCCTGGGAGGGGTGCGGCGCGACATC
ACGCCGGAACAGATCGAGATGATTCGTAATTCCGTGAACACCCTGGAGGAGCGGACAAAGTACTACATCGAGGTGGCAAC
GGAAGAACCGACCCTGGCGGCTCGTCTCGCCGGAGTGGGCTACCTGTCCCACGAGGATGCCCTGCTGCTCTCGGCGGTTG
GACCAACCCTGCGGGCATCCAACGTGGACCGCGACATCCGCAGGGACGACCCCTACGCCGCCTACGGGAGCCTGGACTTC
AGGGTGATCACCGACGACCACTGCGACGTGTACGGACGGACCCTGGTCAGAGTCGGGGAGCTAATGGAGTCCTTCAGGAT
GATCCGCCAGATACTGAACGAACTGCCGGAGGGAGAGGTGTCGGTCAAGGCGCCGCGCAAGATACCGGCCGGCGAGGCCT
GCTCCCGCTACGAAGCCCCGCGGGGAGAATGTTTCCACTACATCAGGGCCAACGGCACGGATAAACCGGAGCGGGTCAAG
GTGCGTGCCTCCACCTTCGCCAACATCCAGGCGGTGGCCAAGATGATGGAAAACAACAACCTGGCGGACGTGCCGATCAT
CGTTGCCGCCATCGACCCCTGCTTCTCCTGTGCCGATCGCTGTCTGGAACTGTTGGGTGAGGGGAGGCAGCTGATGGAAT
GGAGCGAGCTGCGCCGTCGGGGCGTGGAATGGTACCGCCGCAACCGGGGGATCGATTTCAGCGGCCCGAGCCGCAGGCTG
CAGATGCTGCGTGGAGACAGACGGCCATGA

Upstream 100 bases:

>100_bases
CCGGCCCCCGCTACCCGCTGCCGGATGACTGGCCCGAGGGCCAGTACCCCCTGCGCAAGGAGTGGAAGACGGAAATGCTT
GACAGGAAGGAGTGATTGCC

Downstream 100 bases:

>100_bases
CACAGGCACTCTTCAATCTTCTGATATTCCCCGGTTTTCTCTTCCTCAGCCTGGCCGGGATGGTGGCGGAATTCTGCGAC
AGGAAGTTGTACGCCCGCAT

Product: NADH dehydrogenase (ubiquinone)

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSPVTIPIGPQHPALKEPANFSITVTGEKIVTTSMRLGYNHRGIEKACEERSYLQCLYLLERICGICSHTHSTAYIQAVE
EIAGLTIPPRANYIRSLVGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDILAELGGNRVNYGINTLGGVRRDI
TPEQIEMIRNSVNTLEERTKYYIEVATEEPTLAARLAGVGYLSHEDALLLSAVGPTLRASNVDRDIRRDDPYAAYGSLDF
RVITDDHCDVYGRTLVRVGELMESFRMIRQILNELPEGEVSVKAPRKIPAGEACSRYEAPRGECFHYIRANGTDKPERVK
VRASTFANIQAVAKMMENNNLADVPIIVAAIDPCFSCADRCLELLGEGRQLMEWSELRRRGVEWYRRNRGIDFSGPSRRL
QMLRGDRRP

Sequences:

>Translated_409_residues
MSPVTIPIGPQHPALKEPANFSITVTGEKIVTTSMRLGYNHRGIEKACEERSYLQCLYLLERICGICSHTHSTAYIQAVE
EIAGLTIPPRANYIRSLVGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDILAELGGNRVNYGINTLGGVRRDI
TPEQIEMIRNSVNTLEERTKYYIEVATEEPTLAARLAGVGYLSHEDALLLSAVGPTLRASNVDRDIRRDDPYAAYGSLDF
RVITDDHCDVYGRTLVRVGELMESFRMIRQILNELPEGEVSVKAPRKIPAGEACSRYEAPRGECFHYIRANGTDKPERVK
VRASTFANIQAVAKMMENNNLADVPIIVAAIDPCFSCADRCLELLGEGRQLMEWSELRRRGVEWYRRNRGIDFSGPSRRL
QMLRGDRRP
>Mature_408_residues
SPVTIPIGPQHPALKEPANFSITVTGEKIVTTSMRLGYNHRGIEKACEERSYLQCLYLLERICGICSHTHSTAYIQAVEE
IAGLTIPPRANYIRSLVGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDILAELGGNRVNYGINTLGGVRRDIT
PEQIEMIRNSVNTLEERTKYYIEVATEEPTLAARLAGVGYLSHEDALLLSAVGPTLRASNVDRDIRRDDPYAAYGSLDFR
VITDDHCDVYGRTLVRVGELMESFRMIRQILNELPEGEVSVKAPRKIPAGEACSRYEAPRGECFHYIRANGTDKPERVKV
RASTFANIQAVAKMMENNNLADVPIIVAAIDPCFSCADRCLELLGEGRQLMEWSELRRRGVEWYRRNRGIDFSGPSRRLQ
MLRGDRRP

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=384, Percent_Identity=28.6458333333333, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI260898743, Length=373, Percent_Identity=28.4182305630027, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1789076, Length=387, Percent_Identity=37.7260981912145, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1788832, Length=388, Percent_Identity=36.8556701030928, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI145693162, Length=381, Percent_Identity=28.0839895013123, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17568379, Length=380, Percent_Identity=28.9473684210526, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17555284, Length=380, Percent_Identity=28.9473684210526, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI221459469, Length=382, Percent_Identity=29.0575916230367, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24638644, Length=384, Percent_Identity=28.90625, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 46191; Mature: 46059

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVTIPIGPQHPALKEPANFSITVTGEKIVTTSMRLGYNHRGIEKACEERSYLQCLYLL
CCCCEECCCCCCCCCCCCCCEEEEEECCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERICGICSHTHSTAYIQAVEEIAGLTIPPRANYIRSLVGELERIHSHLLWLGVAGHEIGF
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
DTLLMYSWRDRELVMDILAELGGNRVNYGINTLGGVRRDITPEQIEMIRNSVNTLEERTK
HHEEEECCCCHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCE
YYIEVATEEPTLAARLAGVGYLSHEDALLLSAVGPTLRASNVDRDIRRDDPYAAYGSLDF
EEEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCCCCHHHCCCCCEEECCEEE
RVITDDHCDVYGRTLVRVGELMESFRMIRQILNELPEGEVSVKAPRKIPAGEACSRYEAP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCC
RGECFHYIRANGTDKPERVKVRASTFANIQAVAKMMENNNLADVPIIVAAIDPCFSCADR
CCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCHHHHHHHH
CLELLGEGRQLMEWSELRRRGVEWYRRNRGIDFSGPSRRLQMLRGDRRP
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPVTIPIGPQHPALKEPANFSITVTGEKIVTTSMRLGYNHRGIEKACEERSYLQCLYLL
CCCEECCCCCCCCCCCCCCEEEEEECCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERICGICSHTHSTAYIQAVEEIAGLTIPPRANYIRSLVGELERIHSHLLWLGVAGHEIGF
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
DTLLMYSWRDRELVMDILAELGGNRVNYGINTLGGVRRDITPEQIEMIRNSVNTLEERTK
HHEEEECCCCHHHHHHHHHHHCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCE
YYIEVATEEPTLAARLAGVGYLSHEDALLLSAVGPTLRASNVDRDIRRDDPYAAYGSLDF
EEEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCCCCHHHCCCCCEEECCEEE
RVITDDHCDVYGRTLVRVGELMESFRMIRQILNELPEGEVSVKAPRKIPAGEACSRYEAP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHCCCC
RGECFHYIRANGTDKPERVKVRASTFANIQAVAKMMENNNLADVPIIVAAIDPCFSCADR
CCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEHHCHHHHHHHH
CLELLGEGRQLMEWSELRRRGVEWYRRNRGIDFSGPSRRLQMLRGDRRP
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]