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The map label for this gene is dapA

Identifier: 118581466

GI number: 118581466

Start: 3352023

End: 3352895

Strand: Reverse

Name: dapA

Synonym: Ppro_3063

Alternate gene names: 118581466

Gene position: 3352895-3352023 (Counterclockwise)

Preceding gene: 118581467

Following gene: 118581465

Centisome position: 83.66

GC content: 61.4

Gene sequence:

>873_bases
ATGTTCAAAGGAAGTATTGTTGCCCTGGTTACGCCGTTCAGGGACGGCGCCGTTGATGAAGAAACGCTGCGCCGGATGGT
GGACTACCAGATCGAGAACGGGACCGATGGTATTTCGCCATGCGGTACCACCGGTGAGGCCTCGACCCTGGACTATGACG
AACACATGAGGGTGATCGAAATAGTCATCCAGCAGGTAAACGGGCGGGTGCCGGTCATCGCCGGCACCGGCTCCAACTCG
ACGACCGAAGCCATCGAGCTCAGCCGCAAGGCAAAGAAGTTGGGGGCCGACGGCGTCCTGCTGGTTTCTCCCTACTACAA
CAAGCCATCCCAGGAAGGGCTCTACCGTCACTTCAGGGCGATCGCCGATGCCGTGGACATCCCGCAGGTGCTCTACAACG
TGCCGGGGCGCACCGGGGTCAACATGCTCCCCCAGACCGTGGCGCGCCTGGCGGAACACGACAGCATCGTGGCCATCAAG
GAGGCGACAGGTTCTTTGCAGCAGGCCTCCGAACTGATGGAGATGTGCGGTGACAAGATCGACGTCATCTCGGGCGACGA
CTTCATCACCTTTCCCATGATGGCCTGCGGCGCAACAGGGGTCATCTCCGTTGTCGCCAACATCATGCCGCGGGAGGTTG
CCCGGCTGGTGGATGCCTTTTATGAAGGCGACATGGACGAGGCCCGCCGTCTGCACCTGAGTTTGCTCAACATCAGCAAC
GCCATGTTCATAGAAACCAACCCGGTGCCGGTCAAGACAGCCGCTGCCCTGATGGGGCTGTGCGCCGAAGAGTTGCGCCT
GCCGCTGGCTCCCCTGGGCGACGCCAGCCGGGCCACCCTGTGCGCTGTCATGAAATCCTACGGCCTGATCTAG

Upstream 100 bases:

>100_bases
TCGGGGCTGGACGCAGGAGGAGAGGGGGGCTGTCCGTCCGCATGGGCGCGATCGGTCTCTTTTCTGTAAGGCTATGCAGG
TGTATGCGGAGGAGCATGCC

Downstream 100 bases:

>100_bases
GATGTGAGGGACACTAATTTCCCGTGACGCGACGTGAACGAATGATTCCAGAGAGGGTAATGCCATGATCAAGATAGCTG
TCTGCGGAGCTGCCGGCCGC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MFKGSIVALVTPFRDGAVDEETLRRMVDYQIENGTDGISPCGTTGEASTLDYDEHMRVIEIVIQQVNGRVPVIAGTGSNS
TTEAIELSRKAKKLGADGVLLVSPYYNKPSQEGLYRHFRAIADAVDIPQVLYNVPGRTGVNMLPQTVARLAEHDSIVAIK
EATGSLQQASELMEMCGDKIDVISGDDFITFPMMACGATGVISVVANIMPREVARLVDAFYEGDMDEARRLHLSLLNISN
AMFIETNPVPVKTAAALMGLCAEELRLPLAPLGDASRATLCAVMKSYGLI

Sequences:

>Translated_290_residues
MFKGSIVALVTPFRDGAVDEETLRRMVDYQIENGTDGISPCGTTGEASTLDYDEHMRVIEIVIQQVNGRVPVIAGTGSNS
TTEAIELSRKAKKLGADGVLLVSPYYNKPSQEGLYRHFRAIADAVDIPQVLYNVPGRTGVNMLPQTVARLAEHDSIVAIK
EATGSLQQASELMEMCGDKIDVISGDDFITFPMMACGATGVISVVANIMPREVARLVDAFYEGDMDEARRLHLSLLNISN
AMFIETNPVPVKTAAALMGLCAEELRLPLAPLGDASRATLCAVMKSYGLI
>Mature_290_residues
MFKGSIVALVTPFRDGAVDEETLRRMVDYQIENGTDGISPCGTTGEASTLDYDEHMRVIEIVIQQVNGRVPVIAGTGSNS
TTEAIELSRKAKKLGADGVLLVSPYYNKPSQEGLYRHFRAIADAVDIPQVLYNVPGRTGVNMLPQTVARLAEHDSIVAIK
EATGSLQQASELMEMCGDKIDVISGDDFITFPMMACGATGVISVVANIMPREVARLVDAFYEGDMDEARRLHLSLLNISN
AMFIETNPVPVKTAAALMGLCAEELRLPLAPLGDASRATLCAVMKSYGLI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=291, Percent_Identity=26.8041237113402, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI13540533, Length=289, Percent_Identity=25.6055363321799, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=46.9178082191781, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI87082415, Length=282, Percent_Identity=34.3971631205674, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786463, Length=285, Percent_Identity=34.0350877192982, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789620, Length=233, Percent_Identity=34.7639484978541, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_PELPD (A1ATI8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902716.1
- ProteinModelPortal:   A1ATI8
- SMR:   A1ATI8
- STRING:   A1ATI8
- GeneID:   4574108
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3063
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- PhylomeDB:   A1ATI8
- ProtClustDB:   PRK03170
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31253; Mature: 31253

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 160-160 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGSIVALVTPFRDGAVDEETLRRMVDYQIENGTDGISPCGTTGEASTLDYDEHMRVIE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IVIQQVNGRVPVIAGTGSNSTTEAIELSRKAKKLGADGVLLVSPYYNKPSQEGLYRHFRA
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
IADAVDIPQVLYNVPGRTGVNMLPQTVARLAEHDSIVAIKEATGSLQQASELMEMCGDKI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCCE
DVISGDDFITFPMMACGATGVISVVANIMPREVARLVDAFYEGDMDEARRLHLSLLNISN
EEECCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AMFIETNPVPVKTAAALMGLCAEELRLPLAPLGDASRATLCAVMKSYGLI
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKGSIVALVTPFRDGAVDEETLRRMVDYQIENGTDGISPCGTTGEASTLDYDEHMRVIE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
IVIQQVNGRVPVIAGTGSNSTTEAIELSRKAKKLGADGVLLVSPYYNKPSQEGLYRHFRA
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
IADAVDIPQVLYNVPGRTGVNMLPQTVARLAEHDSIVAIKEATGSLQQASELMEMCGDKI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCCE
DVISGDDFITFPMMACGATGVISVVANIMPREVARLVDAFYEGDMDEARRLHLSLLNISN
EEECCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
AMFIETNPVPVKTAAALMGLCAEELRLPLAPLGDASRATLCAVMKSYGLI
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA