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The map label for this gene is lysS [H]

Identifier: 118581419

GI number: 118581419

Start: 3292901

End: 3294412

Strand: Direct

Name: lysS [H]

Synonym: Ppro_3014

Alternate gene names: 118581419

Gene position: 3292901-3294412 (Clockwise)

Preceding gene: 118581418

Following gene: 118581420

Centisome position: 82.16

GC content: 60.65

Gene sequence:

>1512_bases
ATGACATTGCCGAGAAAGGCCCCGATCACAATGGAAGAACTGAGCGAACTGCTGCTCCAGAGAAGACGCAAGGTCGACAC
CCTCTGGGAAGCCGGCATTAATCCCTATCCCAACGATTTCAGACCAGAGCACACCTCAGCCGACGTCTTTGCCGCCTATG
GCGATAAGGAGCAGGTAGACGCAGAGCATGACATATTCGTCGTGGCGGGGCGCATCCTGGCGCGCCGCTCCTTCGGCAAG
GCAGCCTTCATCAAGATCCAGGACCGCAAGGGCCGCCTGCAGCTGTACATCAAGAAGGACGAGATTGGCGAACAGGCCTT
CGCGGACTTCGAAACCTTCGACATCGGCGACATCGTCGGCGTTGAAGGTTTCCCCTTCCGCACCAAGACCAACGAACTCT
CCCTGCACGCGAAGAAGATCCGCCTGCTGGTCAAGTCACTGCACCCCCTGCCGGAAAAGTTCCACGGCCTGACCGACGTG
GAAACCCGCTACCGCCAGCGCTACGTCGACCTGATCGTCAACCCGGAATCGCGGGAGATATTCATCAAGCGCTCCCGCAT
CATCAACCTGATCCGCGCCTTCATGACCGCCCGGGACTTCCTGGAGGTGGAAACCCCCATGATGCAACGGATCCCGGGAG
GCGCCACGGCCAAGCCATTCATCACCCATCACAACGCCCTGGACATGCAGCTCTTCCTGCGCATCGCGCCGGAACTGTAC
CTGAAACGGCTGGTCGTGGGGGGCATGGAGCGGGTCTTCGAGATCAACCGCAACTTCAGGAACGAGGGGATCTCCGTCCG
CCACAACCCGGAATTCACCATGATGGAGTTCTACCAGGCCTACGCCACCTACGAGGACCTGATGAACTTCACCGAGGAAC
TGTTCTGCCATGTGGCCCAAGAGGTCCTGGGAACCCTGGACTTCGTCAACCAGGGCAGGGAGATCAGTTTCCAGCGCCCC
TGGAAGCGCCTGACCGTGCGGGATGCCATCCTGGAGTACGGCGACATCGACGCCAAAAAACTGGATGATCGCGACCTGGC
CCTGATCTATGCCCGCAGCATCGGCCTGGACCTGCCCGACGACATCGGCCATGGCAAGCTCATGATGGAGATCTTCGAGG
AAGTGGCCGAGCACAAGCTGATCCAGCCCACCTTCGTCACCGCCTACCCCACCGAGGTATCGCCGCTCTCCCGCAAGAAC
GACCACAACCCGGACATCGTGGACCGCTTCGAGCTGATCGTCGGCGGCAGGGAGATCGCCAACGCCTTCTCCGAGCTGAA
CGATCCGGTGGACCAGAAGGAGCGCTTCCTCTCCCAAGTGGCCGAAAAGGCCAAGGGGGACGAAGAGGCCCACTACATGG
ACGAGGACTACATCCGAGCCCTGGAATACGGCCTCCCCCCCACCGCCGGCGAAGGGATTGGTATCGACAGGCTGGTGATG
CTGTTGACCGACTCGGCCTCGATCCGCGACGTCATCCTCTTCCCGCAGCTGCGAAAGGAGAAACGGGATTAG

Upstream 100 bases:

>100_bases
ACATTGAAACAGGGAACGCTCCACCCGGGGCTCCCCTTTTTTGCTTGACGTTACGACACATCGGCGATATTTTTAGCAGT
CTTTTCCCCTGCTATCCCAG

Downstream 100 bases:

>100_bases
GGAAACGGATAACGGAAACCAGGGACCGGGGACGGGTAAAAGTTTTTACCGTTCTCCGGTCCCCGGTCCCCGGCTCCCGG
TTCCCGGTCCCCGCTTTACC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTLPRKAPITMEELSELLLQRRRKVDTLWEAGINPYPNDFRPEHTSADVFAAYGDKEQVDAEHDIFVVAGRILARRSFGK
AAFIKIQDRKGRLQLYIKKDEIGEQAFADFETFDIGDIVGVEGFPFRTKTNELSLHAKKIRLLVKSLHPLPEKFHGLTDV
ETRYRQRYVDLIVNPESREIFIKRSRIINLIRAFMTARDFLEVETPMMQRIPGGATAKPFITHHNALDMQLFLRIAPELY
LKRLVVGGMERVFEINRNFRNEGISVRHNPEFTMMEFYQAYATYEDLMNFTEELFCHVAQEVLGTLDFVNQGREISFQRP
WKRLTVRDAILEYGDIDAKKLDDRDLALIYARSIGLDLPDDIGHGKLMMEIFEEVAEHKLIQPTFVTAYPTEVSPLSRKN
DHNPDIVDRFELIVGGREIANAFSELNDPVDQKERFLSQVAEKAKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVM
LLTDSASIRDVILFPQLRKEKRD

Sequences:

>Translated_503_residues
MTLPRKAPITMEELSELLLQRRRKVDTLWEAGINPYPNDFRPEHTSADVFAAYGDKEQVDAEHDIFVVAGRILARRSFGK
AAFIKIQDRKGRLQLYIKKDEIGEQAFADFETFDIGDIVGVEGFPFRTKTNELSLHAKKIRLLVKSLHPLPEKFHGLTDV
ETRYRQRYVDLIVNPESREIFIKRSRIINLIRAFMTARDFLEVETPMMQRIPGGATAKPFITHHNALDMQLFLRIAPELY
LKRLVVGGMERVFEINRNFRNEGISVRHNPEFTMMEFYQAYATYEDLMNFTEELFCHVAQEVLGTLDFVNQGREISFQRP
WKRLTVRDAILEYGDIDAKKLDDRDLALIYARSIGLDLPDDIGHGKLMMEIFEEVAEHKLIQPTFVTAYPTEVSPLSRKN
DHNPDIVDRFELIVGGREIANAFSELNDPVDQKERFLSQVAEKAKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVM
LLTDSASIRDVILFPQLRKEKRD
>Mature_502_residues
TLPRKAPITMEELSELLLQRRRKVDTLWEAGINPYPNDFRPEHTSADVFAAYGDKEQVDAEHDIFVVAGRILARRSFGKA
AFIKIQDRKGRLQLYIKKDEIGEQAFADFETFDIGDIVGVEGFPFRTKTNELSLHAKKIRLLVKSLHPLPEKFHGLTDVE
TRYRQRYVDLIVNPESREIFIKRSRIINLIRAFMTARDFLEVETPMMQRIPGGATAKPFITHHNALDMQLFLRIAPELYL
KRLVVGGMERVFEINRNFRNEGISVRHNPEFTMMEFYQAYATYEDLMNFTEELFCHVAQEVLGTLDFVNQGREISFQRPW
KRLTVRDAILEYGDIDAKKLDDRDLALIYARSIGLDLPDDIGHGKLMMEIFEEVAEHKLIQPTFVTAYPTEVSPLSRKND
HNPDIVDRFELIVGGREIANAFSELNDPVDQKERFLSQVAEKAKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVML
LTDSASIRDVILFPQLRKEKRD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=505, Percent_Identity=42.1782178217822, Blast_Score=371, Evalue=1e-102,
Organism=Homo sapiens, GI194272210, Length=505, Percent_Identity=42.1782178217822, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI45439306, Length=467, Percent_Identity=22.0556745182013, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=57.2008113590264, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789256, Length=493, Percent_Identity=58.0121703853955, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=32.6153846153846, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788173, Length=247, Percent_Identity=25.1012145748988, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=513, Percent_Identity=42.1052631578947, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535927, Length=513, Percent_Identity=42.1052631578947, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=508, Percent_Identity=42.3228346456693, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17551876, Length=538, Percent_Identity=21.9330855018587, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=209, Percent_Identity=24.4019138755981, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=508, Percent_Identity=42.1259842519685, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324256, Length=484, Percent_Identity=30.5785123966942, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323011, Length=480, Percent_Identity=23.75, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=329, Percent_Identity=24.6200607902736, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=195, Percent_Identity=29.7435897435897, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=42.4180327868852, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=42.4180327868852, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136276, Length=466, Percent_Identity=20.8154506437768, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24584738, Length=265, Percent_Identity=25.2830188679245, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58111; Mature: 57980

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRKAPITMEELSELLLQRRRKVDTLWEAGINPYPNDFRPEHTSADVFAAYGDKEQVD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHCC
AEHDIFVVAGRILARRSFGKAAFIKIQDRKGRLQLYIKKDEIGEQAFADFETFDIGDIVG
CCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHHCCHHHHHCCCCCCCCCCCC
VEGFPFRTKTNELSLHAKKIRLLVKSLHPLPEKFHGLTDVETRYRQRYVDLIVNPESREI
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHEECCCCCEE
FIKRSRIINLIRAFMTARDFLEVETPMMQRIPGGATAKPFITHHNALDMQLFLRIAPELY
EHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
LKRLVVGGMERVFEINRNFRNEGISVRHNPEFTMMEFYQAYATYEDLMNFTEELFCHVAQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLGTLDFVNQGREISFQRPWKRLTVRDAILEYGDIDAKKLDDRDLALIYARSIGLDLPD
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEEHHCCCCCCC
DIGHGKLMMEIFEEVAEHKLIQPTFVTAYPTEVSPLSRKNDHNPDIVDRFELIVGGREIA
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHH
NAFSELNDPVDQKERFLSQVAEKAKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVM
HHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHH
LLTDSASIRDVILFPQLRKEKRD
HHCCCCCHHHEEECHHHHHHCCC
>Mature Secondary Structure 
TLPRKAPITMEELSELLLQRRRKVDTLWEAGINPYPNDFRPEHTSADVFAAYGDKEQVD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHCC
AEHDIFVVAGRILARRSFGKAAFIKIQDRKGRLQLYIKKDEIGEQAFADFETFDIGDIVG
CCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHHCCHHHHHCCCCCCCCCCCC
VEGFPFRTKTNELSLHAKKIRLLVKSLHPLPEKFHGLTDVETRYRQRYVDLIVNPESREI
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHEECCCCCEE
FIKRSRIINLIRAFMTARDFLEVETPMMQRIPGGATAKPFITHHNALDMQLFLRIAPELY
EHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHH
LKRLVVGGMERVFEINRNFRNEGISVRHNPEFTMMEFYQAYATYEDLMNFTEELFCHVAQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLGTLDFVNQGREISFQRPWKRLTVRDAILEYGDIDAKKLDDRDLALIYARSIGLDLPD
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEEEEHHCCCCCCC
DIGHGKLMMEIFEEVAEHKLIQPTFVTAYPTEVSPLSRKNDHNPDIVDRFELIVGGREIA
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHH
NAFSELNDPVDQKERFLSQVAEKAKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVM
HHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHH
LLTDSASIRDVILFPQLRKEKRD
HHCCCCCHHHEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA