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The map label for this gene is rne [H]

Identifier: 118581418

GI number: 118581418

Start: 3290201

End: 3292801

Strand: Direct

Name: rne [H]

Synonym: Ppro_3013

Alternate gene names: 118581418

Gene position: 3290201-3292801 (Clockwise)

Preceding gene: 118581412

Following gene: 118581419

Centisome position: 82.09

GC content: 63.9

Gene sequence:

>2601_bases
ATGAGCAAGGCCGTAAGCAAAAAGATGCTGATCAACGTCATGCACCCCGAAGAGGCCCGCGTGGCCATCGTGCATGACGG
ACGTCTGATGGAACTGAATATAGAGATAAGCGGCAAGGAGCAGACCAAGGGAAACATCTACAAAGGGGTCGTGCTGCGCG
TGGAACCGGGGCTACAGGCCGCCTTCGTGGACATCGGCCGCCCCAAGCCCGGCTTCCTGCAGATGGGCGAACTGCACCCC
GACTTCTGGCAGTGGCGGGACGACGTGCCCGAGGAGCAGCGCAAGCGCCGTCCCCGCATCCAGGAGGTGCTGCGCCGCGG
CCAGGAACTGGTGGTGCAGGTGGAGAAGGACGAGCGCGACAACAAGGGCTCGGCCCTGACCAGCTACCTCTCCCTGCCGG
GGCGCTACATGGTGCTCATGCCGGGAAGCGACTCCAGCGGCATCTCCCGCAAGGTGGAGCAGGAGAGCGTGCGCAAGCAG
TTGAAAGAGATCATGGCCGGGATGAGCATCCCCGAGGGGATCGGCTATATCGTACGCACCCAGGCGGTGGGGCGCACCCC
CGAGGAACTGCAGAAGGACATGGAAAACCTGCTGGCCATGTACGAGGGGATCAAAAAGAGCGCCACCGAGATAAAAGGGG
CCGGCGAAATCTACCGCGACAAGGGGCTGATCATCCGCTTCATCCGCGACTACTTCTCCGACGACATCGACGAGGTGGTG
GTGGACAGCAAGGAGGCCTTCAACGAGGCCAGGGACTTCTTCCGCGAAACCATGCCGGGCTGCGAAAAGCTGGTCAAGCT
GCACAAGGAGAAACGGCCGGTCTTCTCCCGCTTCCAGATCGAGGAGCAGATCGACCAGATCTACGAGAAGCGGGTATCGC
TCCCCTCCGGCGGCTCCCTGATCATCGAACCGACCGAAGCCCTGGTCAGCATCGACGTCAACTCCGGGAAATCCAGCGGC
GAGCGGGGCATCGAGGACACCGCCTTCAAGACCAACATGGAGGCTGCCGAAGAGGTGGCCCGCCAGTTACGTCTGCGCGA
CCTGGGCGGTCTGATCATGATCGACTTCATCGACATGCGCGACCGCAAACATAACCACGAGGTGGAGAAGACCCTCAAGC
AGGCCCTCAAGATGGACAAGGCACGGGTCAACCTGGGGCGCATCTCCGAGTTCGGCATCCTGGAGATGTCCCGCCAGCGC
ATCGCCAAGACCTTGAACGACGCCATCCACCTGGCGTGCCCCCACTGCGAGGGGCGCGGCAAGGTCAAATCGGTGGAGGC
CATGGCGCTCTCCTTTCTGCGCAAGGTGCATGGCGCGGCGGCCAAGGGAAACGTATGCGAGGTGCATGGCGGCCTGCCGC
TGGAAGTGGCCTACTACCTGCTCAACCGCAAGAAGCGGGAACTGGCCCAGATCGAAAATGACTACGACATCGAAGTAACG
GTCAAGGGGAAGACATCCTTCCTGCTCAACCAGATGGAGCTGGAGATCGTCAAACGAGAGAAAAAGCGGCCCGAAGAGCC
GGCCAGGGACGATGCGGACAACGGAGAGGCGACCCAACCGGCGCTCGCCGTCGAGACCGTCGCTGCTGTGCCCCAGGAGG
GGGAGCCATCCCCGGAAAACGGGGCCACCGAAGGGGGCAAGAAGCGCAAACGCCGTCGCAAGAAGAAGAAATCAGCGACG
GAGGGCACCGCGATACCCGGACAGACGGAGGCAGAATCTGCAACAGAGCAGTCCGAAGAGGAGCATCCTCTGGACCAGGC
GCCAGGGGAGCCCACTGCCGCCCGGCAGGCCGAGGAGCACGAGGTGCCCGGTGCGCCAGCGGAGGAGCAGACTGACGAAG
TAACTACGGAACCCACCGAGGAGGGTGAAGCGGCAGCGGCTCCCAAGAAACGCAGACGTCGGCGCAGCCGCAAGGTGAGC
AAAGGCGCCGAGGAATCGGCAACGGCCGAGGAAAGCGATGAGACGGCGTCGGCCGCGGAACCGCTGGAAGTTGCCCCGGC
GGAGCTGGCTGCGCCTGCCGAACCTCCGGCAGGGGAGACAGCGACGGAACAACCGCAAGCGCCGGAGACCGTTCCGCTGG
AGACGCGCTCCTTCGAGTCCGGCCCCCAGGAAGAGCCGGCCGTAGAAACGACTGCAGTTGTCGCGGCAGCCGGGCCAACC
GCTGTGGAGACGACTCCAGAAGAGGAGAGCACCACGCAAGAAGCCGTAGCCGCAGAAACCGCCACGGCCGAGGCTGCCTT
GAAGCAGCTTGCCCCGCCCGAAGAGACTTCCGGTGAAATCGTCGCGCCGGAGCTCCTGACAGCGGAACTCCTCCCGGAAC
AGCCAGAAACGCCGGAACCGGCGGCCAAACCGAAGCGGGCCAGGGCGCCACGGGCCAGGAAGAAGACCGTGGCAACGGAG
GAACAGCAGGCCGTCGCAACGACGGAGGCTGTTCCCGAGAGCGCTCCCGCCGAGGCCGAACCAGCGGTAAAAAAACCGCG
ACGGCGCACCGCGGCCAAGAAGAGCACACCCGCGGCAGACACGACCGCGGAGCCTGTTACGGAAGCACCGAAACCGGCCC
CACGGAAACGTCGCACCAAAAAAGACGAACAAAACCAATGA

Upstream 100 bases:

>100_bases
TCCCCTCCTTTTCCTTTGCTTTCGTCCCAAAAAACTCTATAATTCATCATTTTTTAATAGTTGTATGAGCTCGTCTCATG
TGAAAAGGATAAACACAACA

Downstream 100 bases:

>100_bases
CATTGAAACAGGGAACGCTCCACCCGGGGCTCCCCTTTTTTGCTTGACGTTACGACACATCGGCGATATTTTTAGCAGTC
TTTTCCCCTGCTATCCCAGA

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MSKAVSKKMLINVMHPEEARVAIVHDGRLMELNIEISGKEQTKGNIYKGVVLRVEPGLQAAFVDIGRPKPGFLQMGELHP
DFWQWRDDVPEEQRKRRPRIQEVLRRGQELVVQVEKDERDNKGSALTSYLSLPGRYMVLMPGSDSSGISRKVEQESVRKQ
LKEIMAGMSIPEGIGYIVRTQAVGRTPEELQKDMENLLAMYEGIKKSATEIKGAGEIYRDKGLIIRFIRDYFSDDIDEVV
VDSKEAFNEARDFFRETMPGCEKLVKLHKEKRPVFSRFQIEEQIDQIYEKRVSLPSGGSLIIEPTEALVSIDVNSGKSSG
ERGIEDTAFKTNMEAAEEVARQLRLRDLGGLIMIDFIDMRDRKHNHEVEKTLKQALKMDKARVNLGRISEFGILEMSRQR
IAKTLNDAIHLACPHCEGRGKVKSVEAMALSFLRKVHGAAAKGNVCEVHGGLPLEVAYYLLNRKKRELAQIENDYDIEVT
VKGKTSFLLNQMELEIVKREKKRPEEPARDDADNGEATQPALAVETVAAVPQEGEPSPENGATEGGKKRKRRRKKKKSAT
EGTAIPGQTEAESATEQSEEEHPLDQAPGEPTAARQAEEHEVPGAPAEEQTDEVTTEPTEEGEAAAAPKKRRRRRSRKVS
KGAEESATAEESDETASAAEPLEVAPAELAAPAEPPAGETATEQPQAPETVPLETRSFESGPQEEPAVETTAVVAAAGPT
AVETTPEEESTTQEAVAAETATAEAALKQLAPPEETSGEIVAPELLTAELLPEQPETPEPAAKPKRARAPRARKKTVATE
EQQAVATTEAVPESAPAEAEPAVKKPRRRTAAKKSTPAADTTAEPVTEAPKPAPRKRRTKKDEQNQ

Sequences:

>Translated_866_residues
MSKAVSKKMLINVMHPEEARVAIVHDGRLMELNIEISGKEQTKGNIYKGVVLRVEPGLQAAFVDIGRPKPGFLQMGELHP
DFWQWRDDVPEEQRKRRPRIQEVLRRGQELVVQVEKDERDNKGSALTSYLSLPGRYMVLMPGSDSSGISRKVEQESVRKQ
LKEIMAGMSIPEGIGYIVRTQAVGRTPEELQKDMENLLAMYEGIKKSATEIKGAGEIYRDKGLIIRFIRDYFSDDIDEVV
VDSKEAFNEARDFFRETMPGCEKLVKLHKEKRPVFSRFQIEEQIDQIYEKRVSLPSGGSLIIEPTEALVSIDVNSGKSSG
ERGIEDTAFKTNMEAAEEVARQLRLRDLGGLIMIDFIDMRDRKHNHEVEKTLKQALKMDKARVNLGRISEFGILEMSRQR
IAKTLNDAIHLACPHCEGRGKVKSVEAMALSFLRKVHGAAAKGNVCEVHGGLPLEVAYYLLNRKKRELAQIENDYDIEVT
VKGKTSFLLNQMELEIVKREKKRPEEPARDDADNGEATQPALAVETVAAVPQEGEPSPENGATEGGKKRKRRRKKKKSAT
EGTAIPGQTEAESATEQSEEEHPLDQAPGEPTAARQAEEHEVPGAPAEEQTDEVTTEPTEEGEAAAAPKKRRRRRSRKVS
KGAEESATAEESDETASAAEPLEVAPAELAAPAEPPAGETATEQPQAPETVPLETRSFESGPQEEPAVETTAVVAAAGPT
AVETTPEEESTTQEAVAAETATAEAALKQLAPPEETSGEIVAPELLTAELLPEQPETPEPAAKPKRARAPRARKKTVATE
EQQAVATTEAVPESAPAEAEPAVKKPRRRTAAKKSTPAADTTAEPVTEAPKPAPRKRRTKKDEQNQ
>Mature_865_residues
SKAVSKKMLINVMHPEEARVAIVHDGRLMELNIEISGKEQTKGNIYKGVVLRVEPGLQAAFVDIGRPKPGFLQMGELHPD
FWQWRDDVPEEQRKRRPRIQEVLRRGQELVVQVEKDERDNKGSALTSYLSLPGRYMVLMPGSDSSGISRKVEQESVRKQL
KEIMAGMSIPEGIGYIVRTQAVGRTPEELQKDMENLLAMYEGIKKSATEIKGAGEIYRDKGLIIRFIRDYFSDDIDEVVV
DSKEAFNEARDFFRETMPGCEKLVKLHKEKRPVFSRFQIEEQIDQIYEKRVSLPSGGSLIIEPTEALVSIDVNSGKSSGE
RGIEDTAFKTNMEAAEEVARQLRLRDLGGLIMIDFIDMRDRKHNHEVEKTLKQALKMDKARVNLGRISEFGILEMSRQRI
AKTLNDAIHLACPHCEGRGKVKSVEAMALSFLRKVHGAAAKGNVCEVHGGLPLEVAYYLLNRKKRELAQIENDYDIEVTV
KGKTSFLLNQMELEIVKREKKRPEEPARDDADNGEATQPALAVETVAAVPQEGEPSPENGATEGGKKRKRRRKKKKSATE
GTAIPGQTEAESATEQSEEEHPLDQAPGEPTAARQAEEHEVPGAPAEEQTDEVTTEPTEEGEAAAAPKKRRRRRSRKVSK
GAEESATAEESDETASAAEPLEVAPAELAAPAEPPAGETATEQPQAPETVPLETRSFESGPQEEPAVETTAVVAAAGPTA
VETTPEEESTTQEAVAAETATAEAALKQLAPPEETSGEIVAPELLTAELLPEQPETPEPAAKPKRARAPRARKKTVATEE
QQAVATTEAVPESAPAEAEPAVKKPRRRTAAKKSTPAADTTAEPVTEAPKPAPRKRRTKKDEQNQ

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=533, Percent_Identity=42.7767354596623, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87082235, Length=443, Percent_Identity=37.9232505643341, Blast_Score=275, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 95354; Mature: 95223

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAVSKKMLINVMHPEEARVAIVHDGRLMELNIEISGKEQTKGNIYKGVVLRVEPGLQA
CCHHHHHHHHEEEECCCCCEEEEEECCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCE
AFVDIGRPKPGFLQMGELHPDFWQWRDDVPEEQRKRRPRIQEVLRRGQELVVQVEKDERD
EEEECCCCCCCCEECCCCCCHHHHHHCCCCHHHHHCCCHHHHHHHCCHHEEEEECCCCCC
NKGSALTSYLSLPGRYMVLMPGSDSSGISRKVEQESVRKQLKEIMAGMSIPEGIGYIVRT
CCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
QAVGRTPEELQKDMENLLAMYEGIKKSATEIKGAGEIYRDKGLIIRFIRDYFSDDIDEVV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCHHHHH
VDSKEAFNEARDFFRETMPGCEKLVKLHKEKRPVFSRFQIEEQIDQIYEKRVSLPSGGSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
IIEPTEALVSIDVNSGKSSGERGIEDTAFKTNMEAAEEVARQLRLRDLGGLIMIDFIDMR
EEECCCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHH
DRKHNHEVEKTLKQALKMDKARVNLGRISEFGILEMSRQRIAKTLNDAIHLACPHCEGRG
CCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
KVKSVEAMALSFLRKVHGAAAKGNVCEVHGGLPLEVAYYLLNRKKRELAQIENDYDIEVT
CHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VKGKTSFLLNQMELEIVKREKKRPEEPARDDADNGEATQPALAVETVAAVPQEGEPSPEN
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GATEGGKKRKRRRKKKKSATEGTAIPGQTEAESATEQSEEEHPLDQAPGEPTAARQAEEH
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCHHC
EVPGAPAEEQTDEVTTEPTEEGEAAAAPKKRRRRRSRKVSKGAEESATAEESDETASAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCC
PLEVAPAELAAPAEPPAGETATEQPQAPETVPLETRSFESGPQEEPAVETTAVVAAAGPT
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCC
AVETTPEEESTTQEAVAAETATAEAALKQLAPPEETSGEIVAPELLTAELLPEQPETPEP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHCCCCCCCCCC
AAKPKRARAPRARKKTVATEEQQAVATTEAVPESAPAEAEPAVKKPRRRTAAKKSTPAAD
CCCCCHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHCCCCCCC
TTAEPVTEAPKPAPRKRRTKKDEQNQ
CCCCCHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SKAVSKKMLINVMHPEEARVAIVHDGRLMELNIEISGKEQTKGNIYKGVVLRVEPGLQA
CHHHHHHHHEEEECCCCCEEEEEECCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCE
AFVDIGRPKPGFLQMGELHPDFWQWRDDVPEEQRKRRPRIQEVLRRGQELVVQVEKDERD
EEEECCCCCCCCEECCCCCCHHHHHHCCCCHHHHHCCCHHHHHHHCCHHEEEEECCCCCC
NKGSALTSYLSLPGRYMVLMPGSDSSGISRKVEQESVRKQLKEIMAGMSIPEGIGYIVRT
CCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEH
QAVGRTPEELQKDMENLLAMYEGIKKSATEIKGAGEIYRDKGLIIRFIRDYFSDDIDEVV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCHHHHH
VDSKEAFNEARDFFRETMPGCEKLVKLHKEKRPVFSRFQIEEQIDQIYEKRVSLPSGGSL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
IIEPTEALVSIDVNSGKSSGERGIEDTAFKTNMEAAEEVARQLRLRDLGGLIMIDFIDMR
EEECCCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHH
DRKHNHEVEKTLKQALKMDKARVNLGRISEFGILEMSRQRIAKTLNDAIHLACPHCEGRG
CCCCCHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
KVKSVEAMALSFLRKVHGAAAKGNVCEVHGGLPLEVAYYLLNRKKRELAQIENDYDIEVT
CHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VKGKTSFLLNQMELEIVKREKKRPEEPARDDADNGEATQPALAVETVAAVPQEGEPSPEN
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GATEGGKKRKRRRKKKKSATEGTAIPGQTEAESATEQSEEEHPLDQAPGEPTAARQAEEH
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCHHC
EVPGAPAEEQTDEVTTEPTEEGEAAAAPKKRRRRRSRKVSKGAEESATAEESDETASAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCHHHCCC
PLEVAPAELAAPAEPPAGETATEQPQAPETVPLETRSFESGPQEEPAVETTAVVAAAGPT
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCC
AVETTPEEESTTQEAVAAETATAEAALKQLAPPEETSGEIVAPELLTAELLPEQPETPEP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHCCCCCCCCCC
AAKPKRARAPRARKKTVATEEQQAVATTEAVPESAPAEAEPAVKKPRRRTAAKKSTPAAD
CCCCCHHCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHCCCCCCC
TTAEPVTEAPKPAPRKRRTKKDEQNQ
CCCCCHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]