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The map label for this gene is prfA [H]

Identifier: 118581341

GI number: 118581341

Start: 3210565

End: 3210918

Strand: Reverse

Name: prfA [H]

Synonym: Ppro_2936

Alternate gene names: 118581341

Gene position: 3210918-3210565 (Counterclockwise)

Preceding gene: 118581342

Following gene: 118581340

Centisome position: 80.11

GC content: 64.97

Gene sequence:

>354_bases
ATGGCCGGCTTCGCCGTGAGCGAGGAGAAGAACCGCTGGCTGCAGCGGGAGATGGCCCGGCTGAACGTACGGGAGGAGGA
TCTGGAGGAACAGTTCGTCCGCTCCTCGGGCAGCGGCGGCCAGCATGTCAACAAGACTTCCTCCTCGGTCTTTCTGCGGC
ATATCCCCAGCGGCATCTGCGTGTCGGCCTCCCGTGAGCGCAGCCAGTCGGTGAACCGTTTCCTGGCACGCCGGGAGCTC
CTGGAGCGCATCGAGGCCCAGACTGGAGCAGAGACCGGAGAAATGCGGCGCATCGCGCGCCTGCGCAAACAGAAGAACCG
TCGCAGAAGGCGGGCAGAGAAGAAGGACGCATGA

Upstream 100 bases:

>100_bases
GTGAGACATCCCGCTGCTTCGTCGAAATGGGGCTCACCCTGGAGGATGTGCTGGCTGCCCAGGTGAGGGCCTTTGGCTGG
CGCTCCGGGAATCTGTGACC

Downstream 100 bases:

>100_bases
TCAGTCACCAAACCCTGAAGCGCCTGGAATTCGACAAGATTCTGGCTGAAATCTCCGGCCATGTTCACAGCGAGGTTACG
GCCGCCCGGCTTATGGATAC

Product: class I peptide chain release factor

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 117; Mature: 116

Protein sequence:

>117_residues
MAGFAVSEEKNRWLQREMARLNVREEDLEEQFVRSSGSGGQHVNKTSSSVFLRHIPSGICVSASRERSQSVNRFLARREL
LERIEAQTGAETGEMRRIARLRKQKNRRRRRAEKKDA

Sequences:

>Translated_117_residues
MAGFAVSEEKNRWLQREMARLNVREEDLEEQFVRSSGSGGQHVNKTSSSVFLRHIPSGICVSASRERSQSVNRFLARREL
LERIEAQTGAETGEMRRIARLRKQKNRRRRRAEKKDA
>Mature_116_residues
AGFAVSEEKNRWLQREMARLNVREEDLEEQFVRSSGSGGQHVNKTSSSVFLRHIPSGICVSASRERSQSVNRFLARRELL
ERIEAQTGAETGEMRRIARLRKQKNRRRRRAEKKDA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI303304971, Length=95, Percent_Identity=41.0526315789474, Blast_Score=73, Evalue=6e-14,
Organism=Homo sapiens, GI219879802, Length=95, Percent_Identity=41.0526315789474, Blast_Score=73, Evalue=6e-14,
Organism=Homo sapiens, GI22748627, Length=95, Percent_Identity=41.0526315789474, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24654113, Length=96, Percent_Identity=45.8333333333333, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921226, Length=91, Percent_Identity=39.5604395604396, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 13598; Mature: 13466

Theoretical pI: Translated: 11.81; Mature: 11.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFAVSEEKNRWLQREMARLNVREEDLEEQFVRSSGSGGQHVNKTSSSVFLRHIPSGIC
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
VSASRERSQSVNRFLARRELLERIEAQTGAETGEMRRIARLRKQKNRRRRRAEKKDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGFAVSEEKNRWLQREMARLNVREEDLEEQFVRSSGSGGQHVNKTSSSVFLRHIPSGIC
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
VSASRERSQSVNRFLARRELLERIEAQTGAETGEMRRIARLRKQKNRRRRRAEKKDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA