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The map label for this gene is cobS [H]

Identifier: 118581214

GI number: 118581214

Start: 3071890

End: 3072645

Strand: Direct

Name: cobS [H]

Synonym: Ppro_2807

Alternate gene names: 118581214

Gene position: 3071890-3072645 (Clockwise)

Preceding gene: 118581213

Following gene: 118581215

Centisome position: 76.64

GC content: 64.02

Gene sequence:

>756_bases
ATGCGGCTGTACATCATTGCCATGCAGTTTCTGACCATCATCCCGCTCCCCTGCAGGCACCTGTGGCAACGGGATGATCT
GGGGCGTTCCCTGGCTGTTTTCCCCCTGGCGGGACTGACCATCGGCGCGCTGCTGGCCGGACTGAATCTGCTGACGGCCC
CCCTTGTGGCCCGCCCACTGAACGATCTCCTGCTGATCACCCTCCTGGCGGCCATGACCGGCGCCCTGCACATGGATGGC
CTGGCAGATGTCTGCGACGGCATCGCGGCCCGGGGAAGCCGCGAGCGTTTCCTGGCAATCATGAAGGATTCCAACGTGGG
CGCCATAGGAGCGGTGGGGCTGGTGCTGGGGATACTGCTCAAATGGCAGGCCCTGACGGTGATTCCCGCTGAGTACAAGT
GGCAAACCCTGCTGCTCTTCCCCACCCTGGCCAGGTACTGCCAGGTGCAGACCATCGTCCATGGCAGAAACGCCCGCCAG
GATGGCCTGGGGTCGGCCATGATCGCCGGCGCCGGCACAAGTCGCCTGCTGGCGGCGGGTGTCCTCACCGCAGCCATCGC
CTGGTTGCTGTTGGGGGTAGCCGGCATTACTGCCCTGGCAGCCGCCATGGTGGCCAGCTGGCTGATCAGGGCCTGGTTTA
ACCGCAAGATCGGCGGCATAACCGGTGACGTAATCGGCTGCATCAACGAGCTGGTGGAGATCCTTACCCTGGTCCTGATC
CCGGTCCTGGTCACGTTTGCATGCACGAAACCGTAG

Upstream 100 bases:

>100_bases
AAGGCACGGGCGCCGCCCTGGCCATGTCCCTGATCGAGGCGGGGGTCAAGACCCTGAACGAGATGGCAACCCGCGAGCAG
GCCGGGGTTACCCACGAATA

Downstream 100 bases:

>100_bases
AGAGGCTACATCCGATCGCCCTGCTGGAATCCTTTTCCGGCGGGGAGTTCTCCCGCTCTTACCTGTTCGAAGACTTAGAA
ACAATCGTGCAGGCCCACAC

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRLYIIAMQFLTIIPLPCRHLWQRDDLGRSLAVFPLAGLTIGALLAGLNLLTAPLVARPLNDLLLITLLAAMTGALHMDG
LADVCDGIAARGSRERFLAIMKDSNVGAIGAVGLVLGILLKWQALTVIPAEYKWQTLLLFPTLARYCQVQTIVHGRNARQ
DGLGSAMIAGAGTSRLLAAGVLTAAIAWLLLGVAGITALAAAMVASWLIRAWFNRKIGGITGDVIGCINELVEILTLVLI
PVLVTFACTKP

Sequences:

>Translated_251_residues
MRLYIIAMQFLTIIPLPCRHLWQRDDLGRSLAVFPLAGLTIGALLAGLNLLTAPLVARPLNDLLLITLLAAMTGALHMDG
LADVCDGIAARGSRERFLAIMKDSNVGAIGAVGLVLGILLKWQALTVIPAEYKWQTLLLFPTLARYCQVQTIVHGRNARQ
DGLGSAMIAGAGTSRLLAAGVLTAAIAWLLLGVAGITALAAAMVASWLIRAWFNRKIGGITGDVIGCINELVEILTLVLI
PVLVTFACTKP
>Mature_251_residues
MRLYIIAMQFLTIIPLPCRHLWQRDDLGRSLAVFPLAGLTIGALLAGLNLLTAPLVARPLNDLLLITLLAAMTGALHMDG
LADVCDGIAARGSRERFLAIMKDSNVGAIGAVGLVLGILLKWQALTVIPAEYKWQTLLLFPTLARYCQVQTIVHGRNARQ
DGLGSAMIAGAGTSRLLAAGVLTAAIAWLLLGVAGITALAAAMVASWLIRAWFNRKIGGITGDVIGCINELVEILTLVLI
PVLVTFACTKP

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=250, Percent_Identity=32, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26637; Mature: 26637

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYIIAMQFLTIIPLPCRHLWQRDDLGRSLAVFPLAGLTIGALLAGLNLLTAPLVARPL
CEEEHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDLLLITLLAAMTGALHMDGLADVCDGIAARGSRERFLAIMKDSNVGAIGAVGLVLGILL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHHH
KWQALTVIPAEYKWQTLLLFPTLARYCQVQTIVHGRNARQDGLGSAMIAGAGTSRLLAAG
HHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHH
VLTAAIAWLLLGVAGITALAAAMVASWLIRAWFNRKIGGITGDVIGCINELVEILTLVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PVLVTFACTKP
HHHHHHHCCCC
>Mature Secondary Structure
MRLYIIAMQFLTIIPLPCRHLWQRDDLGRSLAVFPLAGLTIGALLAGLNLLTAPLVARPL
CEEEHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDLLLITLLAAMTGALHMDGLADVCDGIAARGSRERFLAIMKDSNVGAIGAVGLVLGILL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHHH
KWQALTVIPAEYKWQTLLLFPTLARYCQVQTIVHGRNARQDGLGSAMIAGAGTSRLLAAG
HHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHH
VLTAAIAWLLLGVAGITALAAAMVASWLIRAWFNRKIGGITGDVIGCINELVEILTLVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PVLVTFACTKP
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA