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The map label for this gene is hycF [H]

Identifier: 118581191

GI number: 118581191

Start: 3045275

End: 3045802

Strand: Reverse

Name: hycF [H]

Synonym: Ppro_2784

Alternate gene names: 118581191

Gene position: 3045802-3045275 (Counterclockwise)

Preceding gene: 118581192

Following gene: 118581190

Centisome position: 75.99

GC content: 61.17

Gene sequence:

>528_bases
ATGTCCATACTGAGCAAACTGAAAGAGACCGCGATCTGCCTGAACGCCGGCCGGGTAACCATGCCGTACCCCTTTGAGTC
CAAGCCCGTGCCCGAACGCTTCCGCGGCCGTCCGATCTGGGACCACGTCAAATGCATCGGCTGCGCCGGTTGCGCCAACA
ACTGCCCGTCCCGTGAGATCCTGATCAACGACATCTGCCAGGAGATCCGGATCCTGCACTACCTTGGCCGCCGCTGCACC
TACTGCGGTCGCTGTGCCGACGTCTGCCCTGAAGACGCCATCACCATGAGCCACGAGTTCGAGAACGGCACCAACAAGAT
CACCGATCTTCAGCAGCGTCTGGAACTGTTCATGAGCACCTGCCAGCGCTGCGGCCGCTGCTTCAAGGGGCAGTCACCGC
TGGAAGCGCTGAAGATGAAAGGGTACCGCGCGGACGACCTTGCCAACGACCGCTGGGTCTTCAAATCAACGGCCTACCTC
CCCGGCCAGCCGATCCTCGAAGACCTCACCATAGAACTGGAGGGCTAA

Upstream 100 bases:

>100_bases
TACCGCATCGACCAGGCCATCCGGTACTTCGCGATCATGCTGGCCGTATCCCTGGTAACCCTGGCCGCCGCCAGCTACGG
CTTCTGATAGAGGTGAATGT

Downstream 100 bases:

>100_bases
CGCCGTCATGCTAAAGAATCTTTGCCAGAAAGTGTTCGGTCGTTCGCTGTGGGTTTACCACGCCAACAGCGGCGGGTGCA
ACGGCTGCGACATCGAAGTT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSILSKLKETAICLNAGRVTMPYPFESKPVPERFRGRPIWDHVKCIGCAGCANNCPSREILINDICQEIRILHYLGRRCT
YCGRCADVCPEDAITMSHEFENGTNKITDLQQRLELFMSTCQRCGRCFKGQSPLEALKMKGYRADDLANDRWVFKSTAYL
PGQPILEDLTIELEG

Sequences:

>Translated_175_residues
MSILSKLKETAICLNAGRVTMPYPFESKPVPERFRGRPIWDHVKCIGCAGCANNCPSREILINDICQEIRILHYLGRRCT
YCGRCADVCPEDAITMSHEFENGTNKITDLQQRLELFMSTCQRCGRCFKGQSPLEALKMKGYRADDLANDRWVFKSTAYL
PGQPILEDLTIELEG
>Mature_174_residues
SILSKLKETAICLNAGRVTMPYPFESKPVPERFRGRPIWDHVKCIGCAGCANNCPSREILINDICQEIRILHYLGRRCTY
CGRCADVCPEDAITMSHEFENGTNKITDLQQRLELFMSTCQRCGRCFKGQSPLEALKMKGYRADDLANDRWVFKSTAYLP
GQPILEDLTIELEG

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=146, Percent_Identity=32.8767123287671, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788833, Length=130, Percent_Identity=38.4615384615385, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19870; Mature: 19739

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
10.3 %Cys+Met (Translated Protein)
7.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILSKLKETAICLNAGRVTMPYPFESKPVPERFRGRPIWDHVKCIGCAGCANNCPSREI
CHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCHHH
LINDICQEIRILHYLGRRCTYCGRCADVCPEDAITMSHEFENGTNKITDLQQRLELFMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHH
CQRCGRCFKGQSPLEALKMKGYRADDLANDRWVFKSTAYLPGQPILEDLTIELEG
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SILSKLKETAICLNAGRVTMPYPFESKPVPERFRGRPIWDHVKCIGCAGCANNCPSREI
HHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCHHH
LINDICQEIRILHYLGRRCTYCGRCADVCPEDAITMSHEFENGTNKITDLQQRLELFMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHH
CQRCGRCFKGQSPLEALKMKGYRADDLANDRWVFKSTAYLPGQPILEDLTIELEG
HHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]