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The map label for this gene is hyfI [H]

Identifier: 118581190

GI number: 118581190

Start: 3044728

End: 3045267

Strand: Reverse

Name: hyfI [H]

Synonym: Ppro_2783

Alternate gene names: 118581190

Gene position: 3045267-3044728 (Counterclockwise)

Preceding gene: 118581191

Following gene: 118581189

Centisome position: 75.98

GC content: 61.67

Gene sequence:

>540_bases
ATGCTAAAGAATCTTTGCCAGAAAGTGTTCGGTCGTTCGCTGTGGGTTTACCACGCCAACAGCGGCGGGTGCAACGGCTG
CGACATCGAAGTTTTGAACGTCCTCACCCCGTACTATGACGTAGAGCGTTTCGGGATCAAACTGGTTGGTTCCCCCCGTC
ACGCCGACGTCATGCTCTGCCAGGGGCCTGCCATGCGCTCCACCGGCAAGGCACTCCAGCGCGCCTACGCCGCCATGCCC
GGCCCCAAGCTTGTGTTTGCCATCGGCTCCTGCGCCTGCGGCGGCGGCATCTGGCACGACAGCTACGCCGTGCACGGCCC
GGTGGAGAAACTGATCCCGGTCAACTACTACGTTCCCGGCTGCCCTCCGCGTCCTGAAGCCATCATCTGGGGCGTAGCCG
TAGCCGCGGGGCTTGTGGACAAGAAGACCGCGCCGGTGGAATTCAAACAGGTAAGCGACTTCCCCATGCCGCGGTACCAT
CCCGAAGACCTCAGAGGCTCGGGTGACCTGGTGATCTACGAGAAGATGCAGAAGATCTGA

Upstream 100 bases:

>100_bases
GACCTTGCCAACGACCGCTGGGTCTTCAAATCAACGGCCTACCTCCCCGGCCAGCCGATCCTCGAAGACCTCACCATAGA
ACTGGAGGGCTAACGCCGTC

Downstream 100 bases:

>100_bases
CCGGAACGTTACATCCCGGAACACACGCCCCCTTGCGTATCGATCGGCAGCACACGATACACAAGGGGGTTCGTGTTTTA
GCGGCTACGCGATTGAAGGT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLKNLCQKVFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERFGIKLVGSPRHADVMLCQGPAMRSTGKALQRAYAAMP
GPKLVFAIGSCACGGGIWHDSYAVHGPVEKLIPVNYYVPGCPPRPEAIIWGVAVAAGLVDKKTAPVEFKQVSDFPMPRYH
PEDLRGSGDLVIYEKMQKI

Sequences:

>Translated_179_residues
MLKNLCQKVFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERFGIKLVGSPRHADVMLCQGPAMRSTGKALQRAYAAMP
GPKLVFAIGSCACGGGIWHDSYAVHGPVEKLIPVNYYVPGCPPRPEAIIWGVAVAAGLVDKKTAPVEFKQVSDFPMPRYH
PEDLRGSGDLVIYEKMQKI
>Mature_179_residues
MLKNLCQKVFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERFGIKLVGSPRHADVMLCQGPAMRSTGKALQRAYAAMP
GPKLVFAIGSCACGGGIWHDSYAVHGPVEKLIPVNYYVPGCPPRPEAIIWGVAVAAGLVDKKTAPVEFKQVSDFPMPRYH
PEDLRGSGDLVIYEKMQKI

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=131, Percent_Identity=38.1679389312977, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1789074, Length=142, Percent_Identity=46.4788732394366, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1788834, Length=162, Percent_Identity=41.9753086419753, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1788624, Length=137, Percent_Identity=37.956204379562, Blast_Score=97, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=110, Percent_Identity=41.8181818181818, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=42.7272727272727, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=42.7272727272727, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651058, Length=110, Percent_Identity=40, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19552; Mature: 19552

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNLCQKVFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERFGIKLVGSPRHADVMLC
CHHHHHHHHHCCEEEEEECCCCCCCCCCCHHEEHHCCCCCHHHCCEEEECCCCCCEEEEE
QGPAMRSTGKALQRAYAAMPGPKLVFAIGSCACGGGIWHDSYAVHGPVEKLIPVNYYVPG
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHEECEEECCC
CPPRPEAIIWGVAVAAGLVDKKTAPVEFKQVSDFPMPRYHPEDLRGSGDLVIYEKMQKI
CCCCCCCEEHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCEEEEECHHCC
>Mature Secondary Structure
MLKNLCQKVFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERFGIKLVGSPRHADVMLC
CHHHHHHHHHCCEEEEEECCCCCCCCCCCHHEEHHCCCCCHHHCCEEEECCCCCCEEEEE
QGPAMRSTGKALQRAYAAMPGPKLVFAIGSCACGGGIWHDSYAVHGPVEKLIPVNYYVPG
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHEECEEECCC
CPPRPEAIIWGVAVAAGLVDKKTAPVEFKQVSDFPMPRYHPEDLRGSGDLVIYEKMQKI
CCCCCCCEEHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCEEEEECHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]