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The map label for this gene is murF [H]

Identifier: 118581149

GI number: 118581149

Start: 3000366

End: 3001586

Strand: Direct

Name: murF [H]

Synonym: Ppro_2742

Alternate gene names: 118581149

Gene position: 3000366-3001586 (Clockwise)

Preceding gene: 118581148

Following gene: 118581150

Centisome position: 74.86

GC content: 57.58

Gene sequence:

>1221_bases
ATGACGACCCCACGAAAGAAGATCCACTGGCTGAAGACCCGGTGGCAATTCCTTAGATATTTGATATGTCGGCGCTTTTA
TGACAAAACCACGGCAATTGCCGTAACGGGCAGCTGTGGCAAGACCAGCACCACCCATTTTCTGGGAAAAATCCTCTCGG
ACAGAGACTCATGCCGCATTGGCATCTTATTCAACTGCCGGAAGGCTATTATAAAAAACCTGCGCCGGGTGAACAGCTCC
CACCGTTTTGTGCTCCAGGAAGTGGGGGTAGAAGGGACGGGATGCATGAATGAAAGATTACCTCTACTTCGTCCCCGCAT
CGGCATTGTGACAAGTGTCGGCCAGGACCATTACTGCAACTTTCGGACGCTGGAGGCAACCGCGTTCGAAAAAGGAAAGC
TGATCGAATTTCTCCCAGAATCCGGAGTGGCCGTACTCAACGCCGATGATCCCCATGTGCTCGCCATGGCCCGGCGTTCC
AAGGCGCGCGTCCTGACCTACGGGCTATGGGAGGAAGCGGACGTGCGCGGCAGCGACATACGCTGCGCCTGGCCGGAACG
CCTGTCACTGGCCGTTACGTACCAGGGTGAATCCGTACGCATCGAGACCGGTTTGTTCGGAAGCCTGCTGGCCAGTTCCA
TCCTGGCGGCAGTCTGCGGCGCCCTTGCGGCCGGGATCGACCTGAAGCAGTGTGCCGCGTCCCTTCACGCCGTGGAGAGT
TTTCCGCGCAGGATGACGATCCACGCCACCTCGCAACGGGCTTGGTACATCTGCGACACAGCCAAAGCTCCGTACTGGAC
TGTTGCCAAGGTGCTCGAGCCGATGACCGATGCCGCTGCTCCCCGAAAGACGGTCGTGTTCGGCTCGTTTTCAGATACAT
CAGGCTCATCCTCAAGCAAATACCGCAGCATGGCCAGAGAGGCGCTGAACGTTGCGGACAGGGTCTTTTTCGTTGGAAAC
AAGGCTCAGCACATTAGAAGGTTGCTCACCCCCGAGACGGATGGGCGGCTCTTCATGGTGGAATCACTCCAGGCGGCAGC
CCGTCTTTTGGCCGAGGATGTGGTTGCCGATGAGCTCATCCTGATCAAATCCTCGAACATGGAACATGCCGAACGCCTGT
ATTTCAGTCAAGACCGTCTCCTTAACTGCTGGAAGCCAACCTGCCGCCAGTCCATCAGCTGCGAACACTGTCCGGAGAGC
GGGCTGCGGGCCTCATCCTGA

Upstream 100 bases:

>100_bases
GACACCAAAAACGACAAAACATCAGGTTTGTCTCCCCCTCGACCACATCAGGTTAGGCCACCTCCACCCGGACAGCGCTG
CCAGGAAAGGATTGCGAAAG

Downstream 100 bases:

>100_bases
CAATGCCGTTCCCGCTCCAACGGCTTCCCGGGAATCCTGGGCACGGAACCGCCGTTTCCTGGTTGAAAGCTCGGCCCAAC
TCTGCTATACATGACCCGTT

Product: Mur ligase middle domain-containing protein

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTTPRKKIHWLKTRWQFLRYLICRRFYDKTTAIAVTGSCGKTSTTHFLGKILSDRDSCRIGILFNCRKAIIKNLRRVNSS
HRFVLQEVGVEGTGCMNERLPLLRPRIGIVTSVGQDHYCNFRTLEATAFEKGKLIEFLPESGVAVLNADDPHVLAMARRS
KARVLTYGLWEEADVRGSDIRCAWPERLSLAVTYQGESVRIETGLFGSLLASSILAAVCGALAAGIDLKQCAASLHAVES
FPRRMTIHATSQRAWYICDTAKAPYWTVAKVLEPMTDAAAPRKTVVFGSFSDTSGSSSSKYRSMAREALNVADRVFFVGN
KAQHIRRLLTPETDGRLFMVESLQAAARLLAEDVVADELILIKSSNMEHAERLYFSQDRLLNCWKPTCRQSISCEHCPES
GLRASS

Sequences:

>Translated_406_residues
MTTPRKKIHWLKTRWQFLRYLICRRFYDKTTAIAVTGSCGKTSTTHFLGKILSDRDSCRIGILFNCRKAIIKNLRRVNSS
HRFVLQEVGVEGTGCMNERLPLLRPRIGIVTSVGQDHYCNFRTLEATAFEKGKLIEFLPESGVAVLNADDPHVLAMARRS
KARVLTYGLWEEADVRGSDIRCAWPERLSLAVTYQGESVRIETGLFGSLLASSILAAVCGALAAGIDLKQCAASLHAVES
FPRRMTIHATSQRAWYICDTAKAPYWTVAKVLEPMTDAAAPRKTVVFGSFSDTSGSSSSKYRSMAREALNVADRVFFVGN
KAQHIRRLLTPETDGRLFMVESLQAAARLLAEDVVADELILIKSSNMEHAERLYFSQDRLLNCWKPTCRQSISCEHCPES
GLRASS
>Mature_405_residues
TTPRKKIHWLKTRWQFLRYLICRRFYDKTTAIAVTGSCGKTSTTHFLGKILSDRDSCRIGILFNCRKAIIKNLRRVNSSH
RFVLQEVGVEGTGCMNERLPLLRPRIGIVTSVGQDHYCNFRTLEATAFEKGKLIEFLPESGVAVLNADDPHVLAMARRSK
ARVLTYGLWEEADVRGSDIRCAWPERLSLAVTYQGESVRIETGLFGSLLASSILAAVCGALAAGIDLKQCAASLHAVESF
PRRMTIHATSQRAWYICDTAKAPYWTVAKVLEPMTDAAAPRKTVVFGSFSDTSGSSSSKYRSMAREALNVADRVFFVGNK
AQHIRRLLTPETDGRLFMVESLQAAARLLAEDVVADELILIKSSNMEHAERLYFSQDRLLNCWKPTCRQSISCEHCPESG
LRASS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 45332; Mature: 45201

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRKKIHWLKTRWQFLRYLICRRFYDKTTAIAVTGSCGKTSTTHFLGKILSDRDSCRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEE
GILFNCRKAIIKNLRRVNSSHRFVLQEVGVEGTGCMNERLPLLRPRIGIVTSVGQDHYCN
EEEEHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
FRTLEATAFEKGKLIEFLPESGVAVLNADDPHVLAMARRSKARVLTYGLWEEADVRGSDI
EEEEEHHHCCCCCEEEECCCCCEEEEECCCCEEEEEHHCCCCEEEEEECCCCCCCCCCCE
RCAWPERLSLAVTYQGESVRIETGLFGSLLASSILAAVCGALAAGIDLKQCAASLHAVES
EEECCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FPRRMTIHATSQRAWYICDTAKAPYWTVAKVLEPMTDAAAPRKTVVFGSFSDTSGSSSSK
CCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
YRSMAREALNVADRVFFVGNKAQHIRRLLTPETDGRLFMVESLQAAARLLAEDVVADELI
HHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEE
LIKSSNMEHAERLYFSQDRLLNCWKPTCRQSISCEHCPESGLRASS
EEECCCCHHHHHHHHCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPRKKIHWLKTRWQFLRYLICRRFYDKTTAIAVTGSCGKTSTTHFLGKILSDRDSCRI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEE
GILFNCRKAIIKNLRRVNSSHRFVLQEVGVEGTGCMNERLPLLRPRIGIVTSVGQDHYCN
EEEEHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
FRTLEATAFEKGKLIEFLPESGVAVLNADDPHVLAMARRSKARVLTYGLWEEADVRGSDI
EEEEEHHHCCCCCEEEECCCCCEEEEECCCCEEEEEHHCCCCEEEEEECCCCCCCCCCCE
RCAWPERLSLAVTYQGESVRIETGLFGSLLASSILAAVCGALAAGIDLKQCAASLHAVES
EEECCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FPRRMTIHATSQRAWYICDTAKAPYWTVAKVLEPMTDAAAPRKTVVFGSFSDTSGSSSSK
CCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
YRSMAREALNVADRVFFVGNKAQHIRRLLTPETDGRLFMVESLQAAARLLAEDVVADELI
HHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEE
LIKSSNMEHAERLYFSQDRLLNCWKPTCRQSISCEHCPESGLRASS
EEECCCCHHHHHHHHCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]