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The map label for this gene is sigA [H]

Identifier: 118581147

GI number: 118581147

Start: 2998243

End: 2999214

Strand: Direct

Name: sigA [H]

Synonym: Ppro_2739

Alternate gene names: 118581147

Gene position: 2998243-2999214 (Clockwise)

Preceding gene: 118581146

Following gene: 118581148

Centisome position: 74.81

GC content: 60.39

Gene sequence:

>972_bases
ATGAAAAACGAAGACTCTCTGCTGGACAACGGGTTTTCCCTGGATGAGCCTCATGCCGCTTCGGAACTGGTTGCCAACGA
CACCGAAGAGCCGTCGACGTTCAGCGAAGAAGAAGAGGGCAGCGATGACCATGCCGAGGCGGAGCTGCCCCCGGCGGTGC
TGGAAACCTTTGACGACGCCATCAAGATCTATCTGCGGGACATCCAGCGCACCCCGCTTTTGACCGCCGAGCAGGAAAAG
GAGGTGGCCCGGCAGATAGAGAAGGGTGACATGGCCGCCCGCAACAAGATGATCGAGTCCAACCTCAGGTTGGTGGTCAA
GATCGCCAAACGCTACATCAACCGCGGCCTCCCCTTCCTGGACCTGATCGAGGAGGGCAACCTGGGGCTGATCAAGGCGG
TGGAGCGCTTCAACCTGGCCAAAGAGTGCCGCTTTTCCACCTACGCCACCTGGTGGATACGCCAGGCCATCGATCGGGCA
CTGGTCAACCAGTCCCGCACCATCCGCCTGCCGGTTCATGTATCAGACGACATCAACCGCATGGCCAAGGTCACCCGCCG
GCTGTCCCAGACCCTGCAACGTGAGCCCCATGCCCATGAGATCGCCGAGGAGATGAACGTCAAACTGGCCTATGTGCGCA
GGCTGATGACCCTCTTGCGGCGCACCTGTTCCATGGAGACGCCCATCGGCGACGGCAGCGACTACTTCCTGATCGACACC
ATCGAAGACACCTCCATGGTCGCCCCGTCGGACCTGCTGGAGAACATCGATCGCTACGAGCAGATCTCCGAGCACTTCAA
GGAGTTGAGCGAAAGCGAACAGAAGATCCTGGCGCTGCGCTTCGGCCTGGACGACAAGGAACCCCAAACCCTGGACACCA
TCGGCCGAAGCTTTGGCGTAACCAGGGAGCGCATCCGCCAGATCGAGGCCAAGTCTCTGGAAAAGCTGTACGCCTGCGTG
AAGCCCACCTAG

Upstream 100 bases:

>100_bases
TAACCCGCGGCACATGGCCCTGGCGATCTCTCCGCGCTCTTCGCGCCTGGTGTGATTCAGCAACTATTTCAGCTTCACGC
GAAAAGGGGGATATGGGCGA

Downstream 100 bases:

>100_bases
CCGGCAAAGCACGGAGAAGCGGACCAAGCCGAACAATCAAACACAGAGGAGCATTCCCCGTATGATGGAAGACCTGAAGA
GCATCATTCGCGACATCCCC

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKNEDSLLDNGFSLDEPHAASELVANDTEEPSTFSEEEEGSDDHAEAELPPAVLETFDDAIKIYLRDIQRTPLLTAEQEK
EVARQIEKGDMAARNKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNLGLIKAVERFNLAKECRFSTYATWWIRQAIDRA
LVNQSRTIRLPVHVSDDINRMAKVTRRLSQTLQREPHAHEIAEEMNVKLAYVRRLMTLLRRTCSMETPIGDGSDYFLIDT
IEDTSMVAPSDLLENIDRYEQISEHFKELSESEQKILALRFGLDDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLYACV
KPT

Sequences:

>Translated_323_residues
MKNEDSLLDNGFSLDEPHAASELVANDTEEPSTFSEEEEGSDDHAEAELPPAVLETFDDAIKIYLRDIQRTPLLTAEQEK
EVARQIEKGDMAARNKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNLGLIKAVERFNLAKECRFSTYATWWIRQAIDRA
LVNQSRTIRLPVHVSDDINRMAKVTRRLSQTLQREPHAHEIAEEMNVKLAYVRRLMTLLRRTCSMETPIGDGSDYFLIDT
IEDTSMVAPSDLLENIDRYEQISEHFKELSESEQKILALRFGLDDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLYACV
KPT
>Mature_323_residues
MKNEDSLLDNGFSLDEPHAASELVANDTEEPSTFSEEEEGSDDHAEAELPPAVLETFDDAIKIYLRDIQRTPLLTAEQEK
EVARQIEKGDMAARNKMIESNLRLVVKIAKRYINRGLPFLDLIEEGNLGLIKAVERFNLAKECRFSTYATWWIRQAIDRA
LVNQSRTIRLPVHVSDDINRMAKVTRRLSQTLQREPHAHEIAEEMNVKLAYVRRLMTLLRRTCSMETPIGDGSDYFLIDT
IEDTSMVAPSDLLENIDRYEQISEHFKELSESEQKILALRFGLDDKEPQTLDTIGRSFGVTRERIRQIEAKSLEKLYACV
KPT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=47.8723404255319, Blast_Score=257, Evalue=7e-70,
Organism=Escherichia coli, GI1789448, Length=255, Percent_Identity=46.2745098039216, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1789871, Length=281, Percent_Identity=30.2491103202847, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37068; Mature: 37068

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEDSLLDNGFSLDEPHAASELVANDTEEPSTFSEEEEGSDDHAEAELPPAVLETFDDA
CCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IKIYLRDIQRTPLLTAEQEKEVARQIEKGDMAARNKMIESNLRLVVKIAKRYINRGLPFL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DLIEEGNLGLIKAVERFNLAKECRFSTYATWWIRQAIDRALVNQSRTIRLPVHVSDDINR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHH
MAKVTRRLSQTLQREPHAHEIAEEMNVKLAYVRRLMTLLRRTCSMETPIGDGSDYFLIDT
HHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
IEDTSMVAPSDLLENIDRYEQISEHFKELSESEQKILALRFGLDDKEPQTLDTIGRSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCH
TRERIRQIEAKSLEKLYACVKPT
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNEDSLLDNGFSLDEPHAASELVANDTEEPSTFSEEEEGSDDHAEAELPPAVLETFDDA
CCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IKIYLRDIQRTPLLTAEQEKEVARQIEKGDMAARNKMIESNLRLVVKIAKRYINRGLPFL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DLIEEGNLGLIKAVERFNLAKECRFSTYATWWIRQAIDRALVNQSRTIRLPVHVSDDINR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHH
MAKVTRRLSQTLQREPHAHEIAEEMNVKLAYVRRLMTLLRRTCSMETPIGDGSDYFLIDT
HHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
IEDTSMVAPSDLLENIDRYEQISEHFKELSESEQKILALRFGLDDKEPQTLDTIGRSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCH
TRERIRQIEAKSLEKLYACVKPT
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]