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The map label for this gene is pcm1

Identifier: 118581146

GI number: 118581146

Start: 2997413

End: 2998060

Strand: Direct

Name: pcm1

Synonym: Ppro_2738

Alternate gene names: 118581146

Gene position: 2997413-2998060 (Clockwise)

Preceding gene: 118581145

Following gene: 118581147

Centisome position: 74.79

GC content: 62.19

Gene sequence:

>648_bases
ATGAATTTCGATAGAGCACGTAAAAAGATGGTTCAGGAACAGATCGCGACCCGCGGAATCACCGACCGCCGGGTGCTGGA
CGCCATGCTGAAAACACCGCGCCACATCTTCGTCCAGGAGGCCATGGCGGCCCAGGCCTACAGCGACTCTTCACTGCCGG
TGGGGGAGAAGCAGACCATATCCCAGCCTTATACCGTGGCACTGATGACCGAACTGCTGGAGTTGACCGGCAGGGAAAAG
GTGCTGGAGATCGGCACCGGTTCCGGCTACCAGACGGCTATCCTGGCCGCCCTGGCCGATCGCGTCTATACCGTGGAGCG
GATCCGCCCCCTGGCCCTGCGCGCCAGAAAGTGCCTGGACTCCCTGCGCCTGTTCAACGTCATGCTGCGCATCAACGACA
GCGAGGGGAGCCCCATCGGCTGGGATGAGGAGGCCCCCTTCGACGCCATCATCGTCACCGCCGGGGCTCCGGCGGTTCCC
CAGGTACTCACGGACCAGTTGGCCGTTGACGGCCGCCTGGTTATTCCGGTGGGAGACGAACGGGAACAACGTTTGGTGAA
AATTGTCAGGAAGAACGACGGAACACTGGAAACGACGACATCCATCGGCTGCCGCTTCGTGCCGCTGATCGGCAGACAGG
GGTGGTAG

Upstream 100 bases:

>100_bases
ACGCCACTGCACCTGGACCTGACCAACTACGCATCCATGAAGGTGATCACCACCTGGGTTTTTTAGCGCCGCCGGCCCGT
GCGGGCACCTGGAGCATTGC

Downstream 100 bases:

>100_bases
CGCCCGCGTGCTTCACAACAGCGAGCGGGCGAAGATACAGCTATCACCGACAGAGATGACGGGTCGGCCTGCCACGGCGA
GATAACCCGCGGCACATGGC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNFDRARKKMVQEQIATRGITDRRVLDAMLKTPRHIFVQEAMAAQAYSDSSLPVGEKQTISQPYTVALMTELLELTGREK
VLEIGTGSGYQTAILAALADRVYTVERIRPLALRARKCLDSLRLFNVMLRINDSEGSPIGWDEEAPFDAIIVTAGAPAVP
QVLTDQLAVDGRLVIPVGDEREQRLVKIVRKNDGTLETTTSIGCRFVPLIGRQGW

Sequences:

>Translated_215_residues
MNFDRARKKMVQEQIATRGITDRRVLDAMLKTPRHIFVQEAMAAQAYSDSSLPVGEKQTISQPYTVALMTELLELTGREK
VLEIGTGSGYQTAILAALADRVYTVERIRPLALRARKCLDSLRLFNVMLRINDSEGSPIGWDEEAPFDAIIVTAGAPAVP
QVLTDQLAVDGRLVIPVGDEREQRLVKIVRKNDGTLETTTSIGCRFVPLIGRQGW
>Mature_215_residues
MNFDRARKKMVQEQIATRGITDRRVLDAMLKTPRHIFVQEAMAAQAYSDSSLPVGEKQTISQPYTVALMTELLELTGREK
VLEIGTGSGYQTAILAALADRVYTVERIRPLALRARKCLDSLRLFNVMLRINDSEGSPIGWDEEAPFDAIIVTAGAPAVP
QVLTDQLAVDGRLVIPVGDEREQRLVKIVRKNDGTLETTTSIGCRFVPLIGRQGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=207, Percent_Identity=33.8164251207729, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=49.5098039215686, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=207, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=205, Percent_Identity=29.7560975609756, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17981723, Length=204, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT1_PELPD (A1ASL8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902396.1
- ProteinModelPortal:   A1ASL8
- SMR:   A1ASL8
- STRING:   A1ASL8
- GeneID:   4574568
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2738
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YMVARMS
- PhylomeDB:   A1ASL8
- ProtClustDB:   CLSK828403
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23850; Mature: 23850

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDRARKKMVQEQIATRGITDRRVLDAMLKTPRHIFVQEAMAAQAYSDSSLPVGEKQTI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHC
SQPYTVALMTELLELTGREKVLEIGTGSGYQTAILAALADRVYTVERIRPLALRARKCLD
CCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLRLFNVMLRINDSEGSPIGWDEEAPFDAIIVTAGAPAVPQVLTDQLAVDGRLVIPVGDE
HHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCH
REQRLVKIVRKNDGTLETTTSIGCRFVPLIGRQGW
HHHHHHHHHHCCCCCEEEHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MNFDRARKKMVQEQIATRGITDRRVLDAMLKTPRHIFVQEAMAAQAYSDSSLPVGEKQTI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHC
SQPYTVALMTELLELTGREKVLEIGTGSGYQTAILAALADRVYTVERIRPLALRARKCLD
CCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLRLFNVMLRINDSEGSPIGWDEEAPFDAIIVTAGAPAVPQVLTDQLAVDGRLVIPVGDE
HHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCH
REQRLVKIVRKNDGTLETTTSIGCRFVPLIGRQGW
HHHHHHHHHHCCCCCEEEHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA