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The map label for this gene is ybjF [C]

Identifier: 118580724

GI number: 118580724

Start: 2491555

End: 2493036

Strand: Reverse

Name: ybjF [C]

Synonym: Ppro_2309

Alternate gene names: 118580724

Gene position: 2493036-2491555 (Counterclockwise)

Preceding gene: 118580725

Following gene: 118580723

Centisome position: 62.2

GC content: 63.23

Gene sequence:

>1482_bases
ATGAAAAGAGAGAAACAGAGTAGCGGTTCAAGGCGTAAGGGGGACGGAACGGTAGGTCCCGCGGGCGGCAAGCCGGGCGG
AGAGCGCCCCCTCCGCGTCAGGCTGTCCCCTAGATCGACCGTCGAGGCACGGATCTCGGGGCTGGATGACGAGGGGTACG
GCATCTCCTGCGCCGAGAACCATGGGCTCAGGATCGCCGGTGGTCTGCCGGGGGATACGGTTCAGGCTGTTATCGATCAC
GTGGCCCAGCGCATGGCCTTTGGCCACGTGAAGAAAATTCTCGTCGCCTCGCCCCTGCGCAGCAGGCGCCCCCCCTGCCG
GGAGAGCGCCCACTGTCTCGGCTGCCCGCTGATCGCCATGAACTACTGTGACCAGGCGGAGTGGAAACGGCAATTCGTGC
TCAGGCAGCTGGACGCCTATCGCGGGCTGGATGGGATAGAGCTTCACCCGCTCCTCTCGCCCAGGCGCCTGACCAATTAT
CGCACCACCGTCCGCCTGACCATCGCCGGCAAGTACGCCGAGCCGTACATAGGCATCTTCCGCCGTTCCTCCCACGACGT
GGTCGACCTGTGCGATTGCCCGATCCATCATCACCTGGTCAACCGGGTGGTGGAGGTGGTCAGGCGCGGCATCTCCCGGC
TCAAGGTGCCGGTGTACAGCCCCCGCAACAGGGTCGGTCTTTTGCGCTACCTGGTGGTGAGGGTCTCCGAGGCTGAACAG
AAGGCCATGGTCGTGTTCGTCACCGCCCGACGCTCGTACAACGAGATTCATCACCTGGCCAGGTACGTGCGCGAGCAACT
GCCCGAGGTGGAGGTTGTCGCCCAGAACGTCAACAGCTCCGAGGGAAACGTCATCATGGGGCCTGTGGATCACTTTCTCA
CGCCGAAACACCACCTCACCGAGCGCATCGGCGAGGTAAGCCTGATGATCTCTCCCCGCTCCTTCCTGCAGGTTAACCGC
GACGGCGCCTGTCTGATCTACGAACGGGTGGCCGAATGGGCGCGGCTCACGGGACGGGAAACAGTGCTGGACCTCTACTG
CGGTATCGGTGGCATCGCCCTGACCCTGGCGCGCCGTGCGCGCAGGGTGATCGGCGTGGAGGTGGTTGAGGCGGCCGTGG
AGGACGCTCGCAGGAACGCCCGCATGAACGGCATCTCCAATTGCGTCTTCCAGGCGGGAGACGTGGCCGAGCAGCTTGAG
GAGCTTGCTGAGGATGGCGAAGGGGTGGATGTGGTCGTGCTCAACCCACCCCGCAAAGGGTGTGACGAGCAAGTACTGCG
GCAGGTGGCCGCTCTCGGGCCGCGCACGGTGCTCTATGTCTCCTGTTCCCCCCAAACCTTGGCCCGCGATCTCAACCTGC
TGAAAAGCTTGGGATACGTCTGCGTGGAGGTTCAGCCGGTGGACATGTTTCCCCATACCATGCATGTGGAGAACGTTGCC
CGACTGGAAAAAGTGATAAAAGCGCTTGACAATACGGAATGA

Upstream 100 bases:

>100_bases
CGGCATCAGCCATCGTGGAGAGGCGCTGCGGGGGTTTCGGGAGTACCTGTGTGGCTGCCCGGGTTTCTGTGGCGCTGGAG
AGGGACAGGAGTAAACGTCG

Downstream 100 bases:

>100_bases
TTACTGATATTAACTCATTCGCTTTCGGTTACAGGCGCGTAGCTCAGGGGGAGAGCGCTACCTTGACACGGTAGAGGTCG
GCGGTTCGAGACCGCCCGCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKREKQSSGSRRKGDGTVGPAGGKPGGERPLRVRLSPRSTVEARISGLDDEGYGISCAENHGLRIAGGLPGDTVQAVIDH
VAQRMAFGHVKKILVASPLRSRRPPCRESAHCLGCPLIAMNYCDQAEWKRQFVLRQLDAYRGLDGIELHPLLSPRRLTNY
RTTVRLTIAGKYAEPYIGIFRRSSHDVVDLCDCPIHHHLVNRVVEVVRRGISRLKVPVYSPRNRVGLLRYLVVRVSEAEQ
KAMVVFVTARRSYNEIHHLARYVREQLPEVEVVAQNVNSSEGNVIMGPVDHFLTPKHHLTERIGEVSLMISPRSFLQVNR
DGACLIYERVAEWARLTGRETVLDLYCGIGGIALTLARRARRVIGVEVVEAAVEDARRNARMNGISNCVFQAGDVAEQLE
ELAEDGEGVDVVVLNPPRKGCDEQVLRQVAALGPRTVLYVSCSPQTLARDLNLLKSLGYVCVEVQPVDMFPHTMHVENVA
RLEKVIKALDNTE

Sequences:

>Translated_493_residues
MKREKQSSGSRRKGDGTVGPAGGKPGGERPLRVRLSPRSTVEARISGLDDEGYGISCAENHGLRIAGGLPGDTVQAVIDH
VAQRMAFGHVKKILVASPLRSRRPPCRESAHCLGCPLIAMNYCDQAEWKRQFVLRQLDAYRGLDGIELHPLLSPRRLTNY
RTTVRLTIAGKYAEPYIGIFRRSSHDVVDLCDCPIHHHLVNRVVEVVRRGISRLKVPVYSPRNRVGLLRYLVVRVSEAEQ
KAMVVFVTARRSYNEIHHLARYVREQLPEVEVVAQNVNSSEGNVIMGPVDHFLTPKHHLTERIGEVSLMISPRSFLQVNR
DGACLIYERVAEWARLTGRETVLDLYCGIGGIALTLARRARRVIGVEVVEAAVEDARRNARMNGISNCVFQAGDVAEQLE
ELAEDGEGVDVVVLNPPRKGCDEQVLRQVAALGPRTVLYVSCSPQTLARDLNLLKSLGYVCVEVQPVDMFPHTMHVENVA
RLEKVIKALDNTE
>Mature_493_residues
MKREKQSSGSRRKGDGTVGPAGGKPGGERPLRVRLSPRSTVEARISGLDDEGYGISCAENHGLRIAGGLPGDTVQAVIDH
VAQRMAFGHVKKILVASPLRSRRPPCRESAHCLGCPLIAMNYCDQAEWKRQFVLRQLDAYRGLDGIELHPLLSPRRLTNY
RTTVRLTIAGKYAEPYIGIFRRSSHDVVDLCDCPIHHHLVNRVVEVVRRGISRLKVPVYSPRNRVGLLRYLVVRVSEAEQ
KAMVVFVTARRSYNEIHHLARYVREQLPEVEVVAQNVNSSEGNVIMGPVDHFLTPKHHLTERIGEVSLMISPRSFLQVNR
DGACLIYERVAEWARLTGRETVLDLYCGIGGIALTLARRARRVIGVEVVEAAVEDARRNARMNGISNCVFQAGDVAEQLE
ELAEDGEGVDVVVLNPPRKGCDEQVLRQVAALGPRTVLYVSCSPQTLARDLNLLKSLGYVCVEVQPVDMFPHTMHVENVA
RLEKVIKALDNTE

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=315, Percent_Identity=29.2063492063492, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI51173878, Length=315, Percent_Identity=29.2063492063492, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI269784680, Length=421, Percent_Identity=28.0285035629454, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI269784676, Length=421, Percent_Identity=28.0285035629454, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI20149688, Length=421, Percent_Identity=28.0285035629454, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI269784678, Length=337, Percent_Identity=29.9703264094956, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787083, Length=385, Percent_Identity=28.3116883116883, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789148, Length=465, Percent_Identity=28.6021505376344, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1790403, Length=164, Percent_Identity=31.0975609756098, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=167, Percent_Identity=31.1377245508982, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=453, Percent_Identity=26.9315673289183, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 54846; Mature: 54846

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREKQSSGSRRKGDGTVGPAGGKPGGERPLRVRLSPRSTVEARISGLDDEGYGISCAEN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCEEEECC
HGLRIAGGLPGDTVQAVIDHVAQRMAFGHVKKILVASPLRSRRPPCRESAHCLGCPLIAM
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHCCHHHHH
NYCDQAEWKRQFVLRQLDAYRGLDGIELHPLLSPRRLTNYRTTVRLTIAGKYAEPYIGIF
HHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCCEEEEEEEEECCCCCCHHHHH
RRSSHDVVDLCDCPIHHHLVNRVVEVVRRGISRLKVPVYSPRNRVGLLRYLVVRVSEAEQ
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
KAMVVFVTARRSYNEIHHLARYVREQLPEVEVVAQNVNSSEGNVIMGPVDHFLTPKHHLT
CEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCEEECCHHHHCCCHHHHH
ERIGEVSLMISPRSFLQVNRDGACLIYERVAEWARLTGRETVLDLYCGIGGIALTLARRA
HHHCCEEEEECCHHHHEECCCCCEEHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHH
RRVIGVEVVEAAVEDARRNARMNGISNCVFQAGDVAEQLEELAEDGEGVDVVVLNPPRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
CDEQVLRQVAALGPRTVLYVSCSPQTLARDLNLLKSLGYVCVEVQPVDMFPHTMHVENVA
CHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHH
RLEKVIKALDNTE
HHHHHHHHHCCCC
>Mature Secondary Structure
MKREKQSSGSRRKGDGTVGPAGGKPGGERPLRVRLSPRSTVEARISGLDDEGYGISCAEN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCEEEECC
HGLRIAGGLPGDTVQAVIDHVAQRMAFGHVKKILVASPLRSRRPPCRESAHCLGCPLIAM
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHCCHHHHH
NYCDQAEWKRQFVLRQLDAYRGLDGIELHPLLSPRRLTNYRTTVRLTIAGKYAEPYIGIF
HHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCCEEEEEEEEECCCCCCHHHHH
RRSSHDVVDLCDCPIHHHLVNRVVEVVRRGISRLKVPVYSPRNRVGLLRYLVVRVSEAEQ
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
KAMVVFVTARRSYNEIHHLARYVREQLPEVEVVAQNVNSSEGNVIMGPVDHFLTPKHHLT
CEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCEEECCHHHHCCCHHHHH
ERIGEVSLMISPRSFLQVNRDGACLIYERVAEWARLTGRETVLDLYCGIGGIALTLARRA
HHHCCEEEEECCHHHHEECCCCCEEHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHH
RRVIGVEVVEAAVEDARRNARMNGISNCVFQAGDVAEQLEELAEDGEGVDVVVLNPPRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
CDEQVLRQVAALGPRTVLYVSCSPQTLARDLNLLKSLGYVCVEVQPVDMFPHTMHVENVA
CHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHH
RLEKVIKALDNTE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA