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The map label for this gene is nadE [H]

Identifier: 118580580

GI number: 118580580

Start: 2333043

End: 2335112

Strand: Reverse

Name: nadE [H]

Synonym: Ppro_2164

Alternate gene names: 118580580

Gene position: 2335112-2333043 (Counterclockwise)

Preceding gene: 118580581

Following gene: 118580577

Centisome position: 58.26

GC content: 64.06

Gene sequence:

>2070_bases
GTGGATACGACATTCTTCAATCTCTACAACCATAATTTCATCCGTGCGGCAGTGGCGGTCCCCGAGGTCAAGGTGGCAGA
CCCGGCACACAACGCAGGTCAGACCATCGCCATGATGCGCCAGGCGTGGGAGCAGAGCGCGCTGATCGCGATCTTTCCCG
AACTGGGGCTCTCGGCCTACTCCTGCGACGACCTGTTTCACCAGCAGACCCTGCTGGACGGCTCCATGGATGCACTGGAC
GCGGTGCTGAAGGCCTCCATGGACATTCCGGTGATCACGGTGGTGGGACTGCCGCTGCAGGTCCACAGCATGCTGTTCAA
CTGCGCCGTGGTGCTCTACCGGGGCAGGATCCTGGGGGTGGCGCCCAAGAGTTTCCTCCCCGGCTACCGCGAGTTCTACG
AGCTGCGCCAGTTCGTCCCGGCCGCCTACGCTCCGGTGGAGAAGATCGACCTGCTGGGGCAGCAGGGGATCCCCTTCGGC
AACCGCCTGCTGTTCCAGGTGGAGGAACAGCCGCTGCTGACCTTCTACACCGAAATCTGCGAGGATGTGTGGGTGCCCAT
TCCCCCTTCCTCCTTTGCGGCCCTGGCCGGGGCCAGCGTCCTGATCAACCTCTCCGCCTCCAACATCACCGTGGGCAAGG
CCGATTACCGGCGCAGCCTGGTGGCCAACCAGTCCGGCCGCTGTCTGTCCGCCTATCTCTACAGCGCCGCAGGAATCGGG
GAATCCACCACCGACCTGGCCTGGGACGGACACGGCATGATCTTCGAGAATGGCGCCTGCCTAGGGGAAACCAAGCGTTT
CTCCTATGAATCCCAGATGATCTTCGCTGATATCGACCTTGACAGGCTGGCCCAGGAACGCATGCGCCAGACCAGCTACG
GCCAGTCGGTGCTCCGGCACAGGGATGAAGTGGCCGGTTTCCGCACGGTACGCTTTTCAGCCTCCCTGCCCAGGGAACGG
GTGCTCCCCCTGGAGCGATTCTACGAACGCTTCCCCTTCGTACCCAGCGACCCGTCGCGGCGGGACGAGCGCTGCAAGGA
GGTGTACGAGATCCAGGTGCAGGGGCTGGTGAAGCGCTTCAAGGCAACCGGAGCGGACAAGATGGTGATCGGCGTCTCCG
GCGGCCTGGACTCCACCCAGGCGCTCCTGGTCTGCGCCCGGGCCATGGATGTCATGGGGCTCTCCCGGATGCATATCCTG
GCCTACACCATGCCCGGATTCGCCACCAGCCAGCGTACCCTGGAACAGGCCAGGGAACTGATCCGGACGGTTGGCTGCAC
CTACCACGAGATCGATATCCGTCCCAGTTGCCTGCAGATGCTCCAGGACATCGGCCACCCCTTCTTCCACGGCAAGCCGG
TCTACGACATCACCTTCGAGAACGTGCAGGCCGGCGAGAGGACCAGCCACCTCTTCCGGTTGGCCAACCAGACCGGCGGC
CTGGTGGTGGGGACCAGCGATCTGAGCGAGCTGGGGCTTGGGTGGTGCACCTACGGCGTAGGAGATCACATGGCCCACTA
CCATGTCAATGCCAGCGTTCCCAAGACCCTGATCCAGTACCTGATTCGCTGGGCCGCCCTCACCCACCAGTTGGGTCCGG
AGGTGAGCACGGTGCTCATGGACGTGCTGGAGACCGAGATCAGCCCCGAGCTGGTTCCGGGCAAGGAGGACGAAGATCAG
CCGGTGCAGCGCACCGAGGATGTGGTCGGCCCCTATGAGCTGCAGGACTTCAACCTCTACTACATCCTGCGCCTGGGCTA
CCTGCCGCCCAAGGTCGCCTTCCTGGCCTGGAACGCCTGGCACGACCGGACGCGCCACCATTGGCCGGATATCCCCGAAC
ACAAGCGCAACCAGTACTCCATGCACGACATCAAGCGCTGGCTGACGGTCTTTCTGCGTCGCTTCTTCAAGACCAGCCAG
TACAAGCGCAGCTGCATGGCCAATGCTCCCAAGGTCGGCTCGGGCGGATCCCTGTCACCCCGCGGCGACTGGCGCGCCCC
CAGCGACGGCGAGGCCAGCGCCTGGCTCGCCCAGTTGGAGCGCATCCCCGACCAGGCCCATGACGAATGA

Upstream 100 bases:

>100_bases
TGCCTGGATTTCGCCCTCCGCTCAACAATCGTTTTTCAAGCCGGGTGAAACGTGCTAGAGTGGCGCCCATGCAGGCCTTG
AGAACAGGGAGGGAAACCAG

Downstream 100 bases:

>100_bases
TGATCCGCAAAAAAAATCCTCCTTTCCCCAGGGGGATTTTTTTTGCGTGAGTGTCCGCTTCTGGCCATGGCCTGAGAGGC
CCGAATCCGGGAGCGGCATC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MDTTFFNLYNHNFIRAAVAVPEVKVADPAHNAGQTIAMMRQAWEQSALIAIFPELGLSAYSCDDLFHQQTLLDGSMDALD
AVLKASMDIPVITVVGLPLQVHSMLFNCAVVLYRGRILGVAPKSFLPGYREFYELRQFVPAAYAPVEKIDLLGQQGIPFG
NRLLFQVEEQPLLTFYTEICEDVWVPIPPSSFAALAGASVLINLSASNITVGKADYRRSLVANQSGRCLSAYLYSAAGIG
ESTTDLAWDGHGMIFENGACLGETKRFSYESQMIFADIDLDRLAQERMRQTSYGQSVLRHRDEVAGFRTVRFSASLPRER
VLPLERFYERFPFVPSDPSRRDERCKEVYEIQVQGLVKRFKATGADKMVIGVSGGLDSTQALLVCARAMDVMGLSRMHIL
AYTMPGFATSQRTLEQARELIRTVGCTYHEIDIRPSCLQMLQDIGHPFFHGKPVYDITFENVQAGERTSHLFRLANQTGG
LVVGTSDLSELGLGWCTYGVGDHMAHYHVNASVPKTLIQYLIRWAALTHQLGPEVSTVLMDVLETEISPELVPGKEDEDQ
PVQRTEDVVGPYELQDFNLYYILRLGYLPPKVAFLAWNAWHDRTRHHWPDIPEHKRNQYSMHDIKRWLTVFLRRFFKTSQ
YKRSCMANAPKVGSGGSLSPRGDWRAPSDGEASAWLAQLERIPDQAHDE

Sequences:

>Translated_689_residues
MDTTFFNLYNHNFIRAAVAVPEVKVADPAHNAGQTIAMMRQAWEQSALIAIFPELGLSAYSCDDLFHQQTLLDGSMDALD
AVLKASMDIPVITVVGLPLQVHSMLFNCAVVLYRGRILGVAPKSFLPGYREFYELRQFVPAAYAPVEKIDLLGQQGIPFG
NRLLFQVEEQPLLTFYTEICEDVWVPIPPSSFAALAGASVLINLSASNITVGKADYRRSLVANQSGRCLSAYLYSAAGIG
ESTTDLAWDGHGMIFENGACLGETKRFSYESQMIFADIDLDRLAQERMRQTSYGQSVLRHRDEVAGFRTVRFSASLPRER
VLPLERFYERFPFVPSDPSRRDERCKEVYEIQVQGLVKRFKATGADKMVIGVSGGLDSTQALLVCARAMDVMGLSRMHIL
AYTMPGFATSQRTLEQARELIRTVGCTYHEIDIRPSCLQMLQDIGHPFFHGKPVYDITFENVQAGERTSHLFRLANQTGG
LVVGTSDLSELGLGWCTYGVGDHMAHYHVNASVPKTLIQYLIRWAALTHQLGPEVSTVLMDVLETEISPELVPGKEDEDQ
PVQRTEDVVGPYELQDFNLYYILRLGYLPPKVAFLAWNAWHDRTRHHWPDIPEHKRNQYSMHDIKRWLTVFLRRFFKTSQ
YKRSCMANAPKVGSGGSLSPRGDWRAPSDGEASAWLAQLERIPDQAHDE
>Mature_689_residues
MDTTFFNLYNHNFIRAAVAVPEVKVADPAHNAGQTIAMMRQAWEQSALIAIFPELGLSAYSCDDLFHQQTLLDGSMDALD
AVLKASMDIPVITVVGLPLQVHSMLFNCAVVLYRGRILGVAPKSFLPGYREFYELRQFVPAAYAPVEKIDLLGQQGIPFG
NRLLFQVEEQPLLTFYTEICEDVWVPIPPSSFAALAGASVLINLSASNITVGKADYRRSLVANQSGRCLSAYLYSAAGIG
ESTTDLAWDGHGMIFENGACLGETKRFSYESQMIFADIDLDRLAQERMRQTSYGQSVLRHRDEVAGFRTVRFSASLPRER
VLPLERFYERFPFVPSDPSRRDERCKEVYEIQVQGLVKRFKATGADKMVIGVSGGLDSTQALLVCARAMDVMGLSRMHIL
AYTMPGFATSQRTLEQARELIRTVGCTYHEIDIRPSCLQMLQDIGHPFFHGKPVYDITFENVQAGERTSHLFRLANQTGG
LVVGTSDLSELGLGWCTYGVGDHMAHYHVNASVPKTLIQYLIRWAALTHQLGPEVSTVLMDVLETEISPELVPGKEDEDQ
PVQRTEDVVGPYELQDFNLYYILRLGYLPPKVAFLAWNAWHDRTRHHWPDIPEHKRNQYSMHDIKRWLTVFLRRFFKTSQ
YKRSCMANAPKVGSGGSLSPRGDWRAPSDGEASAWLAQLERIPDQAHDE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=553, Percent_Identity=23.5081374321881, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538756, Length=292, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321865, Length=606, Percent_Identity=22.9372937293729, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24641841, Length=245, Percent_Identity=26.9387755102041, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24641843, Length=245, Percent_Identity=26.9387755102041, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 77444; Mature: 77444

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTFFNLYNHNFIRAAVAVPEVKVADPAHNAGQTIAMMRQAWEQSALIAIFPELGLSAY
CCCEEEEECCCCCEEHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHCCCCC
SCDDLFHQQTLLDGSMDALDAVLKASMDIPVITVVGLPLQVHSMLFNCAVVLYRGRILGV
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
APKSFLPGYREFYELRQFVPAAYAPVEKIDLLGQQGIPFGNRLLFQVEEQPLLTFYTEIC
CCHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
EDVWVPIPPSSFAALAGASVLINLSASNITVGKADYRRSLVANQSGRCLSAYLYSAAGIG
HHCCCCCCCCHHHHHCCCEEEEEEECCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCC
ESTTDLAWDGHGMIFENGACLGETKRFSYESQMIFADIDLDRLAQERMRQTSYGQSVLRH
CCCCCCEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
RDEVAGFRTVRFSASLPRERVLPLERFYERFPFVPSDPSRRDERCKEVYEIQVQGLVKRF
HHHHCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KATGADKMVIGVSGGLDSTQALLVCARAMDVMGLSRMHILAYTMPGFATSQRTLEQAREL
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHEEEEECCCCCHHHHHHHHHHHH
IRTVGCTYHEIDIRPSCLQMLQDIGHPFFHGKPVYDITFENVQAGERTSHLFRLANQTGG
HHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCC
LVVGTSDLSELGLGWCTYGVGDHMAHYHVNASVPKTLIQYLIRWAALTHQLGPEVSTVLM
EEEECCCHHHHCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
DVLETEISPELVPGKEDEDQPVQRTEDVVGPYELQDFNLYYILRLGYLPPKVAFLAWNAW
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCEEEEEEECCCCCCCCEEEEHHH
HDRTRHHWPDIPEHKRNQYSMHDIKRWLTVFLRRFFKTSQYKRSCMANAPKVGSGGSLSP
HCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RGDWRAPSDGEASAWLAQLERIPDQAHDE
CCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDTTFFNLYNHNFIRAAVAVPEVKVADPAHNAGQTIAMMRQAWEQSALIAIFPELGLSAY
CCCEEEEECCCCCEEHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHCCCCC
SCDDLFHQQTLLDGSMDALDAVLKASMDIPVITVVGLPLQVHSMLFNCAVVLYRGRILGV
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
APKSFLPGYREFYELRQFVPAAYAPVEKIDLLGQQGIPFGNRLLFQVEEQPLLTFYTEIC
CCHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
EDVWVPIPPSSFAALAGASVLINLSASNITVGKADYRRSLVANQSGRCLSAYLYSAAGIG
HHCCCCCCCCHHHHHCCCEEEEEEECCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCC
ESTTDLAWDGHGMIFENGACLGETKRFSYESQMIFADIDLDRLAQERMRQTSYGQSVLRH
CCCCCCEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
RDEVAGFRTVRFSASLPRERVLPLERFYERFPFVPSDPSRRDERCKEVYEIQVQGLVKRF
HHHHCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KATGADKMVIGVSGGLDSTQALLVCARAMDVMGLSRMHILAYTMPGFATSQRTLEQAREL
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHEEEEECCCCCHHHHHHHHHHHH
IRTVGCTYHEIDIRPSCLQMLQDIGHPFFHGKPVYDITFENVQAGERTSHLFRLANQTGG
HHHHCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCC
LVVGTSDLSELGLGWCTYGVGDHMAHYHVNASVPKTLIQYLIRWAALTHQLGPEVSTVLM
EEEECCCHHHHCCCHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
DVLETEISPELVPGKEDEDQPVQRTEDVVGPYELQDFNLYYILRLGYLPPKVAFLAWNAW
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCEEEEEEECCCCCCCCEEEEHHH
HDRTRHHWPDIPEHKRNQYSMHDIKRWLTVFLRRFFKTSQYKRSCMANAPKVGSGGSLSP
HCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RGDWRAPSDGEASAWLAQLERIPDQAHDE
CCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]