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The map label for this gene is appB [H]

Identifier: 118580430

GI number: 118580430

Start: 2163179

End: 2164156

Strand: Reverse

Name: appB [H]

Synonym: Ppro_2014

Alternate gene names: 118580430

Gene position: 2164156-2163179 (Counterclockwise)

Preceding gene: 118580431

Following gene: 118580429

Centisome position: 54.0

GC content: 62.07

Gene sequence:

>978_bases
ATGCTGACCTATCTGACAAAACGCGTTCTGCTGCTGATACCGCTCATGGTGGGCATAACCCTGATCACCTTCTCCGTGAT
CCATCTTGCGCCGGGAGAACCGGTGGAGATGCAGATGGCAATGAACCCCAAGGTGGGCAAGGAGGCCCGTGAGCGTCTGA
GGGCTTTCTACGGCCTGGACCAGCCGCTGCACGTGCAGTATTTCAGTTGGGTCGGCAAGCTGGCCCGCCTGGACCTGGGA
CGCTCGTTCTCCTCCGACAGCCGCCCGGTTATCGAAAAGATCAGGGAGCGCCTGCCGGTTACCATCTCCCTGAACATCAT
CGCCCTGCTGCTGGAGTTCGGGCTGGCCATCCCCATCGGTATCCTGGCCGCCACCCATCGTGATACCCTGCTGGACCGGG
GAATAACGGTCTTCGTCTTTGTTGGCTTTGCCGTACCGTCCTTCTGGCTGGCGCTGCTGTTGATGTACTTCTTCGGGGTG
AGGCTGAACTGGCTGCCCATATCCGGACTGCATACCCTGGGGAGCGAGGCGTACGGCACCGCCTTCTACCTGTGGGACAT
GGCCAAGCACCTGGTCATGCCGATCATGGTGGCATCCTTCGGCAGCCTGGCCGGACTGTCGCGCTACATGCGCTCCTCCA
TGCTGGGGGTCGTCGGCCAGGATTACATCACCACCGCCCGTGCCAAGGGGCTCTCCGAGCGGGTGGTGATCTACCGCCAT
GCCCTACGCAACGCCCTGCTTCCGTTGATCACCCTGGCCGGTTTCTCCATTCCCGGCCTGATCGGCGGCAGCGTCATCTT
CGAGACCATCTTCGCCATCCCCGGCATGGGGCAGCTCTTCTACCAGGGGGTCATGTCCCGCGACTATCCGGTGGTCATGG
GTATCCTGGTGATCGGCGCCTTTTTGACCCTGATCGGTAACCTGGTGGCCGATGTCAGCTACGCCCTGGCCGATCCGCGC
ATCCGGCACGGGGCATAG

Upstream 100 bases:

>100_bases
GACTCGTACGTACGCAGTATTTTCCCGCGCTGGCCACCGGCCGGCCGGGTGAAGCGCATCACATAGGTGCCGCGCGTGCC
GGTGGCGCAAGGATGGAACC

Downstream 100 bases:

>100_bases
GGGCGCCCATGACCGACCTGACCAGTTCATACCTGTACGCCGTTGTCTGGCAACGCCTGAAACGCAACCGCCTGGCCCTG
GCCGGCGGCTGCGTGGTGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLTYLTKRVLLLIPLMVGITLITFSVIHLAPGEPVEMQMAMNPKVGKEARERLRAFYGLDQPLHVQYFSWVGKLARLDLG
RSFSSDSRPVIEKIRERLPVTISLNIIALLLEFGLAIPIGILAATHRDTLLDRGITVFVFVGFAVPSFWLALLLMYFFGV
RLNWLPISGLHTLGSEAYGTAFYLWDMAKHLVMPIMVASFGSLAGLSRYMRSSMLGVVGQDYITTARAKGLSERVVIYRH
ALRNALLPLITLAGFSIPGLIGGSVIFETIFAIPGMGQLFYQGVMSRDYPVVMGILVIGAFLTLIGNLVADVSYALADPR
IRHGA

Sequences:

>Translated_325_residues
MLTYLTKRVLLLIPLMVGITLITFSVIHLAPGEPVEMQMAMNPKVGKEARERLRAFYGLDQPLHVQYFSWVGKLARLDLG
RSFSSDSRPVIEKIRERLPVTISLNIIALLLEFGLAIPIGILAATHRDTLLDRGITVFVFVGFAVPSFWLALLLMYFFGV
RLNWLPISGLHTLGSEAYGTAFYLWDMAKHLVMPIMVASFGSLAGLSRYMRSSMLGVVGQDYITTARAKGLSERVVIYRH
ALRNALLPLITLAGFSIPGLIGGSVIFETIFAIPGMGQLFYQGVMSRDYPVVMGILVIGAFLTLIGNLVADVSYALADPR
IRHGA
>Mature_325_residues
MLTYLTKRVLLLIPLMVGITLITFSVIHLAPGEPVEMQMAMNPKVGKEARERLRAFYGLDQPLHVQYFSWVGKLARLDLG
RSFSSDSRPVIEKIRERLPVTISLNIIALLLEFGLAIPIGILAATHRDTLLDRGITVFVFVGFAVPSFWLALLLMYFFGV
RLNWLPISGLHTLGSEAYGTAFYLWDMAKHLVMPIMVASFGSLAGLSRYMRSSMLGVVGQDYITTARAKGLSERVVIYRH
ALRNALLPLITLAGFSIPGLIGGSVIFETIFAIPGMGQLFYQGVMSRDYPVVMGILVIGAFLTLIGNLVADVSYALADPR
IRHGA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=323, Percent_Identity=40.2476780185758, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=35.9882005899705, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1787497, Length=323, Percent_Identity=34.984520123839, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1787053, Length=323, Percent_Identity=33.7461300309598, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788504, Length=367, Percent_Identity=32.1525885558583, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787761, Length=337, Percent_Identity=35.6083086053413, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787550, Length=271, Percent_Identity=31.7343173431734, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35845; Mature: 35845

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTYLTKRVLLLIPLMVGITLITFSVIHLAPGEPVEMQMAMNPKVGKEARERLRAFYGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
QPLHVQYFSWVGKLARLDLGRSFSSDSRPVIEKIRERLPVTISLNIIALLLEFGLAIPIG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
ILAATHRDTLLDRGITVFVFVGFAVPSFWLALLLMYFFGVRLNWLPISGLHTLGSEAYGT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECEEECCCHHHHCCHHHHH
AFYLWDMAKHLVMPIMVASFGSLAGLSRYMRSSMLGVVGQDYITTARAKGLSERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALRNALLPLITLAGFSIPGLIGGSVIFETIFAIPGMGQLFYQGVMSRDYPVVMGILVIGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
FLTLIGNLVADVSYALADPRIRHGA
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLTYLTKRVLLLIPLMVGITLITFSVIHLAPGEPVEMQMAMNPKVGKEARERLRAFYGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
QPLHVQYFSWVGKLARLDLGRSFSSDSRPVIEKIRERLPVTISLNIIALLLEFGLAIPIG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
ILAATHRDTLLDRGITVFVFVGFAVPSFWLALLLMYFFGVRLNWLPISGLHTLGSEAYGT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECEEECCCHHHHCCHHHHH
AFYLWDMAKHLVMPIMVASFGSLAGLSRYMRSSMLGVVGQDYITTARAKGLSERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALRNALLPLITLAGFSIPGLIGGSVIFETIFAIPGMGQLFYQGVMSRDYPVVMGILVIGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
FLTLIGNLVADVSYALADPRIRHGA
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]