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The map label for this gene is nifS1 [H]

Identifier: 118580431

GI number: 118580431

Start: 2164493

End: 2165668

Strand: Reverse

Name: nifS1 [H]

Synonym: Ppro_2015

Alternate gene names: 118580431

Gene position: 2165668-2164493 (Counterclockwise)

Preceding gene: 118580432

Following gene: 118580430

Centisome position: 54.03

GC content: 63.69

Gene sequence:

>1176_bases
ATGAGGGAGATCTATCTGGACAACAATGCCACCACCAAGGTTGACGAAGCGGTCTTCGAGGAGATGCGCCCCTATTTCTG
CGAACTGTACGGCAACCCCAGTTCCATGCACTTCTTCGGCGGCCAGGTACAGGGGAAGGTGGTTGAGGCCAGAAACCGCG
TTGCCGGCCTGCTGGGTGCCTCTCCCGAGGAGATCATCTTCACAGCCTGCGGAACCGAGAGCGACAATACCGCCATCCGC
TCGACCCTGGAGGTTCTTCCCAATCGTCGTCACGTGATCACCTCACGCGTGGAGCACCCGGCGGTGCTGACCCAGTGCCG
CAACCTGATCCAGAAGGGGTACCGCGTTACGGAAATCGGCGTGGACGGCGAGGGGCGTCTGAACATGGATGAGTATCGGG
CCGCCGTGGACGACGACACCGCCATCGTGTCGCTTATGTGGGCCAACAACGAAACCGGGGTGATCTTCCCGGTGGAGGAG
GCCGCCGCCCTGGCCAGGGACAAGGGGGCGCTCTTCCATACCGACGCGGTCCAGGCCGTGGGCAAGATCCCCATCGACAT
GTCCTCTTCCGTGGTGGACATGCTCTCCATCTCGGGGCATAAGCTGCATGCACCCAAGGGGATCGGTGTGCTGTACGTGC
GCCGCGGCACGCCGTTCCGCCCCTTTCTCGTGGGGGGGCATCAGGAGAAGAGCCGCCGCGCCGGCACCGAGAATGCCGCC
GCCATCATCGCCCTGGGCAAGGCCTGCGAGCTGGCGGCAGCGCACATGCCGTTCGAGAACAACCAGGTCAAGGCCATGCG
TGACCGTCTGCAGGAGTCGCTCCTGGCTGCCATTCCCCACTCCCGTATCAACGGGGGGGGCGCGGAGCGCTTACCCAACA
CGCTTTCCATCGCCTTCGAGTTCGTGGAAGGGGAGTCCATCCTGATGCTGCTCTCCGAACTGGGCATCTGCGCCTCGTCG
GGCAGCGCCTGCACCTCCGGTTCGCTGGAACCGTCCCATGTGCTGCGCGCCATGGGCGTGCCCTTTACCTGCGCCCACGG
CTCAATCCGCTTCTCGCTCTCTCGCTATACCACAGAAGCCGAGATCGACGGCGTGATCCGTGAACTGCCCCCCATCATCG
AACGCTTGCGGCAGATGTCCCCCTTCGGTCGGGAATTTCTCAAGGCACAGGCCTAG

Upstream 100 bases:

>100_bases
CGTGTGCCGGCTGCGCATCTTCCGGCAACACGGCGCGCATGGTCGAGCACAAGCTGCGCGACCTGGTGGCCGAGGATATC
GTTGTCGAGGAGGTGTCCGC

Downstream 100 bases:

>100_bases
CCGGTCACCACCGCAAACCAGTATCCAGAGAGGGCGGCCCCGTGCCGCCCTTTGTTATGTTGCCGCGCAGCTGGTGTAAG
TGGTATACATGGGCGCGTGC

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MREIYLDNNATTKVDEAVFEEMRPYFCELYGNPSSMHFFGGQVQGKVVEARNRVAGLLGASPEEIIFTACGTESDNTAIR
STLEVLPNRRHVITSRVEHPAVLTQCRNLIQKGYRVTEIGVDGEGRLNMDEYRAAVDDDTAIVSLMWANNETGVIFPVEE
AAALARDKGALFHTDAVQAVGKIPIDMSSSVVDMLSISGHKLHAPKGIGVLYVRRGTPFRPFLVGGHQEKSRRAGTENAA
AIIALGKACELAAAHMPFENNQVKAMRDRLQESLLAAIPHSRINGGGAERLPNTLSIAFEFVEGESILMLLSELGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTEAEIDGVIRELPPIIERLRQMSPFGREFLKAQA

Sequences:

>Translated_391_residues
MREIYLDNNATTKVDEAVFEEMRPYFCELYGNPSSMHFFGGQVQGKVVEARNRVAGLLGASPEEIIFTACGTESDNTAIR
STLEVLPNRRHVITSRVEHPAVLTQCRNLIQKGYRVTEIGVDGEGRLNMDEYRAAVDDDTAIVSLMWANNETGVIFPVEE
AAALARDKGALFHTDAVQAVGKIPIDMSSSVVDMLSISGHKLHAPKGIGVLYVRRGTPFRPFLVGGHQEKSRRAGTENAA
AIIALGKACELAAAHMPFENNQVKAMRDRLQESLLAAIPHSRINGGGAERLPNTLSIAFEFVEGESILMLLSELGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTEAEIDGVIRELPPIIERLRQMSPFGREFLKAQA
>Mature_391_residues
MREIYLDNNATTKVDEAVFEEMRPYFCELYGNPSSMHFFGGQVQGKVVEARNRVAGLLGASPEEIIFTACGTESDNTAIR
STLEVLPNRRHVITSRVEHPAVLTQCRNLIQKGYRVTEIGVDGEGRLNMDEYRAAVDDDTAIVSLMWANNETGVIFPVEE
AAALARDKGALFHTDAVQAVGKIPIDMSSSVVDMLSISGHKLHAPKGIGVLYVRRGTPFRPFLVGGHQEKSRRAGTENAA
AIIALGKACELAAAHMPFENNQVKAMRDRLQESLLAAIPHSRINGGGAERLPNTLSIAFEFVEGESILMLLSELGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTEAEIDGVIRELPPIIERLRQMSPFGREFLKAQA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=41.7098445595855, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=35.8353510895884, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=44.4155844155844, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1789175, Length=398, Percent_Identity=27.1356783919598, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787970, Length=397, Percent_Identity=25.9445843828715, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=388, Percent_Identity=42.0103092783505, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=33.1230283911672, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=42.3772609819121, Blast_Score=304, Evalue=1e-83,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=43.3766233766234, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42554; Mature: 42554

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIYLDNNATTKVDEAVFEEMRPYFCELYGNPSSMHFFGGQVQGKVVEARNRVAGLLGA
CCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
SPEEIIFTACGTESDNTAIRSTLEVLPNRRHVITSRVEHPAVLTQCRNLIQKGYRVTEIG
CCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEC
VDGEGRLNMDEYRAAVDDDTAIVSLMWANNETGVIFPVEEAAALARDKGALFHTDAVQAV
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHCCCCEEEHHHHHHH
GKIPIDMSSSVVDMLSISGHKLHAPKGIGVLYVRRGTPFRPFLVGGHQEKSRRAGTENAA
HCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEECCCCHHHHHCCCCCCE
AIIALGKACELAAAHMPFENNQVKAMRDRLQESLLAAIPHSRINGGGAERLPNTLSIAFE
EEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEE
FVEGESILMLLSELGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTEA
EECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEEHHCCHH
EIDGVIRELPPIIERLRQMSPFGREFLKAQA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MREIYLDNNATTKVDEAVFEEMRPYFCELYGNPSSMHFFGGQVQGKVVEARNRVAGLLGA
CCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
SPEEIIFTACGTESDNTAIRSTLEVLPNRRHVITSRVEHPAVLTQCRNLIQKGYRVTEIG
CCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEC
VDGEGRLNMDEYRAAVDDDTAIVSLMWANNETGVIFPVEEAAALARDKGALFHTDAVQAV
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHCCCCEEEHHHHHHH
GKIPIDMSSSVVDMLSISGHKLHAPKGIGVLYVRRGTPFRPFLVGGHQEKSRRAGTENAA
HCCCCCCCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEECCCCHHHHHCCCCCCE
AIIALGKACELAAAHMPFENNQVKAMRDRLQESLLAAIPHSRINGGGAERLPNTLSIAFE
EEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEE
FVEGESILMLLSELGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTEA
EECCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEEHHCCHH
EIDGVIRELPPIIERLRQMSPFGREFLKAQA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]