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The map label for this gene is rpfG [H]

Identifier: 118579802

GI number: 118579802

Start: 1464363

End: 1465409

Strand: Reverse

Name: rpfG [H]

Synonym: Ppro_1378

Alternate gene names: 118579802

Gene position: 1465409-1464363 (Counterclockwise)

Preceding gene: 118579806

Following gene: 118579800

Centisome position: 36.56

GC content: 57.5

Gene sequence:

>1047_bases
ATGAATGAAAACATACTGATAGTGGATGATGAACTACTGGTCAGGGAACTGCTCACAACGGCGCTGACCTACGAGGGCTT
CACCTGCCATACTGCAACGAATGTGGACGAGGCCTTCGGCATCATGGACGTGCAGCAGATGGATCTGGTCATATCGGACA
TCATGATGCCGGGCAGAAGTGGCATGGAGCTGCTCAGGGATCTGAAGCAGATCGACGAGGACATAGCCGTGCTGATGATC
ACGGGACTGACCGACACCCAAACCGCCCTGGAATGCATCAGCCTGGGGGCTGACGACTATATCGCCAAACCCTTCAGCAT
CACCAGGGTCATACTGACGGTCAGAAACCTGATCGAGCGCCGACAACTTGCCCTGGAAAAAAAAAAGAACCAGGCCAGCC
TGGAATCCAGGGTAATGGCCCAGACCGAGCAGGTGCGAAGCACCATGTTCGAACTCCAGAGTGCGTATAACGACACCTTG
ACGGCGCTGGTCAAGGCGCTTGATGCCCGGGAGAAGGAAGCCGGTTCACATTCGGAACGGGTGATGAACTATTCCAAGCT
GCTTGCGGCCAGAATGGGAGTTGATGCGCACAGGCGGGAGCAACTGGCCAAGGGCGCTCTGCTGCACGACATCGGCAAGA
TCGGCATCACCGACAAAACCCTGCTCAAGCCGAGCGAATTGAATGAACAGGAATGGCGCGAAATACACCGCCACCCCCAG
GTCGGCTACGACATCCTGTCCCAGATACGTTTCCTCAAGGCGCCAGCAGAGATCATCCTCAACCACCACGAGCGCTTCGA
CGGCAGAGGCTATCCCAACGGCCTCAAGGGGGAAGAAATTCCCATCGGCGCCCGCATCTTTGCGCTGGTGGATACCCTGG
ACGCCATGACAAGCGACCGCCCCTATCGCGGGGCACTTCCCTTCGACAGCGTTACCGACGAGGTCACCAGATGCCGCGGC
AGCCAGTTCGACCCGGATATTGCCGATCTGTTCCTCTCCATCCCGCGCTGCGAGTGGGAGGAGTGCAACGACAAGAGGTT
ACTCTGA

Upstream 100 bases:

>100_bases
CAAATTGATGATAGAGTCCTGAAACTACTGGCAGGCATGTGTGGCGGTGAACGCATATCGGAGCACGCCCTTGCGCGACG
CTACCCGATGGAGCAACGGC

Downstream 100 bases:

>100_bases
AACTCTTGCTCCCGGCCCCCCCACCCCTTCACCCCCATTCCGGCCGTCTGAACTCGGTCCAGGGACCAGCCTTGGGCGAT
CCCCCTGGCGCCCCCCCCAT

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNENILIVDDELLVRELLTTALTYEGFTCHTATNVDEAFGIMDVQQMDLVISDIMMPGRSGMELLRDLKQIDEDIAVLMI
TGLTDTQTALECISLGADDYIAKPFSITRVILTVRNLIERRQLALEKKKNQASLESRVMAQTEQVRSTMFELQSAYNDTL
TALVKALDAREKEAGSHSERVMNYSKLLAARMGVDAHRREQLAKGALLHDIGKIGITDKTLLKPSELNEQEWREIHRHPQ
VGYDILSQIRFLKAPAEIILNHHERFDGRGYPNGLKGEEIPIGARIFALVDTLDAMTSDRPYRGALPFDSVTDEVTRCRG
SQFDPDIADLFLSIPRCEWEECNDKRLL

Sequences:

>Translated_348_residues
MNENILIVDDELLVRELLTTALTYEGFTCHTATNVDEAFGIMDVQQMDLVISDIMMPGRSGMELLRDLKQIDEDIAVLMI
TGLTDTQTALECISLGADDYIAKPFSITRVILTVRNLIERRQLALEKKKNQASLESRVMAQTEQVRSTMFELQSAYNDTL
TALVKALDAREKEAGSHSERVMNYSKLLAARMGVDAHRREQLAKGALLHDIGKIGITDKTLLKPSELNEQEWREIHRHPQ
VGYDILSQIRFLKAPAEIILNHHERFDGRGYPNGLKGEEIPIGARIFALVDTLDAMTSDRPYRGALPFDSVTDEVTRCRG
SQFDPDIADLFLSIPRCEWEECNDKRLL
>Mature_348_residues
MNENILIVDDELLVRELLTTALTYEGFTCHTATNVDEAFGIMDVQQMDLVISDIMMPGRSGMELLRDLKQIDEDIAVLMI
TGLTDTQTALECISLGADDYIAKPFSITRVILTVRNLIERRQLALEKKKNQASLESRVMAQTEQVRSTMFELQSAYNDTL
TALVKALDAREKEAGSHSERVMNYSKLLAARMGVDAHRREQLAKGALLHDIGKIGITDKTLLKPSELNEQEWREIHRHPQ
VGYDILSQIRFLKAPAEIILNHHERFDGRGYPNGLKGEEIPIGARIFALVDTLDAMTSDRPYRGALPFDSVTDEVTRCRG
SQFDPDIADLFLSIPRCEWEECNDKRLL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=35.4330708661417, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=35.8974358974359, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786599, Length=207, Percent_Identity=28.0193236714976, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789809, Length=130, Percent_Identity=28.4615384615385, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786784, Length=130, Percent_Identity=31.5384615384615, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=33.8983050847458, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787229, Length=130, Percent_Identity=30, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788394, Length=163, Percent_Identity=29.4478527607362, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787375, Length=116, Percent_Identity=30.1724137931034, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=33.0578512396694, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789402, Length=116, Percent_Identity=29.3103448275862, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790552, Length=118, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39348; Mature: 39348

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENILIVDDELLVRELLTTALTYEGFTCHTATNVDEAFGIMDVQQMDLVISDIMMPGRS
CCCCEEEECHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCC
GMELLRDLKQIDEDIAVLMITGLTDTQTALECISLGADDYIAKPFSITRVILTVRNLIER
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHH
RQLALEKKKNQASLESRVMAQTEQVRSTMFELQSAYNDTLTALVKALDAREKEAGSHSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VMNYSKLLAARMGVDAHRREQLAKGALLHDIGKIGITDKTLLKPSELNEQEWREIHRHPQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHCCCC
VGYDILSQIRFLKAPAEIILNHHERFDGRGYPNGLKGEEIPIGARIFALVDTLDAMTSDR
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PYRGALPFDSVTDEVTRCRGSQFDPDIADLFLSIPRCEWEECNDKRLL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNENILIVDDELLVRELLTTALTYEGFTCHTATNVDEAFGIMDVQQMDLVISDIMMPGRS
CCCCEEEECHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCC
GMELLRDLKQIDEDIAVLMITGLTDTQTALECISLGADDYIAKPFSITRVILTVRNLIER
HHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHH
RQLALEKKKNQASLESRVMAQTEQVRSTMFELQSAYNDTLTALVKALDAREKEAGSHSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VMNYSKLLAARMGVDAHRREQLAKGALLHDIGKIGITDKTLLKPSELNEQEWREIHRHPQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHCCCC
VGYDILSQIRFLKAPAEIILNHHERFDGRGYPNGLKGEEIPIGARIFALVDTLDAMTSDR
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PYRGALPFDSVTDEVTRCRGSQFDPDIADLFLSIPRCEWEECNDKRLL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]