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The map label for this gene is phoA [H]

Identifier: 118579800

GI number: 118579800

Start: 1461999

End: 1463675

Strand: Reverse

Name: phoA [H]

Synonym: Ppro_1376

Alternate gene names: 118579800

Gene position: 1463675-1461999 (Counterclockwise)

Preceding gene: 118579802

Following gene: 118579799

Centisome position: 36.52

GC content: 60.35

Gene sequence:

>1677_bases
ATGGGAGAGACAACTGTTGTTACCGCATTGATCGCCATAGGTTTTGCGCTCAGCGCATGCGCATGCGGTTCACCTGGTGC
GCTAAGCGGGAACCGTGACGCCGGGCAGGTGAAAAACGTCATATTCCTGTTGAGTGACGGCACGGCCAACGAGGCCTGGC
CCCTGGCGCGATGGGTCAAGGGCAAACGCCTTGCTTCCGACGCCATTCTGAGCGGCGCCATCCGGACCTACGGCGCCGAT
TCCATCATTACCGACTCGGCGCCCGGTTCCACCAGTTATGCCACCGGCCAGAAGGGAAGCGACAAGGGCATTGCCGTGTA
TCCCTGGAATGTGACCATTGCGGGCGTTGATTGTGACCCTGCCATGGCATACGTACCCCTGGCCACGGTCTTGGAAGGAG
CCAAACTGACGGGTCGCGCGACGGGTGTCGTGGCAACGTCAAATGTGCAGCATGCATCGCCGGCCGACTTTACGGCACAC
ACCCACGACCGCAGCAATTACCTGGAAATCGGCGAGCAACAGGTATACCAGGATATGGACGTGGTGCTTTCCGGTGGTGA
GCAGTATCTGTTGCCCAAGGGGGTCGGCGGAGGTGTCCGAACGGACAATGAAAACCTGGTCCAGGTGGTCAAAGCCAAGG
GGTATGCGTATGTTACCAACCGTGACCAGATGCTGGCGGCTTCGGGCCAGAAGATCTTTGGCCTGTTTGCCAAAGATGCC
ATGGCCTACGACATCGACCGCGCCACCACTGCTCCCGGCGAGCCGTCACTGGCTGAGATGACCACCAAGGCCCTGGAAAC
CCTGTCCAGCAGCTCAAAAGGCAAAGACAGGGGGTTTTTCCTGTTCGTTGAAGGCTCCAAGATTGACTGGGCGGCCCACG
ACAACGATCCGGCCGGCGTGGTGAGCGATCTGATCGCCTACGACGACGCGGTCAAGGCGGCCCTGGATTTTGCCGAGGCA
GACGGCACTACCCTGGTGATCAGCGTGGCCGACCATACCACCGGTGGCCTGTCCATCGGCGTCCGTTCCGACCCCCACTA
TTCCACGACTGATGATGATTTTGTCGTGGCGCCGATGCGCAAGGCCAGGTTGACCGCCGAGGGACTGGGCAGGCGTATCG
AAAAGAGCAATTCCGAAGCCGCCATCAGGGCAGCCTTTGCCGACAGTTGGGGAATCACCGACCTGAGCGCCGACGAAATC
GCCTCGATCCGGAAATCGCGCCCGGGCAAGGCACTGCAGAAAGTTATGGGGAGCATGCTGTCCAGGCGTGCCCGGCTTGG
CTGGACCACCGGCGGGCACACCGGAGGCGACCCCTACCTGTTCTCCTATGGCCCCGGGCGTATCAGCGGCCTGTGGGAAA
ACAGCGCTCTTGGCACGTATGTGGCCGGCAAACTGGGCTTCACCTTCCCGGAAATCAACAAGCGCCTGTTTGTCGAGGCT
TCGGCAGCCTTCCGGGCAGAAGGCTTTTCCACCAGCATCGACCTAAGCGATGCAACCAATCCCGTGTTGACGGTCAGCAA
GGGGACTGCAACGGCGCGGTTGCCATTGGCCAAAAACCTGGTGCTGGTGAACGGCAAAACAATCGAACTGGAAGGCATCG
TGGTTATGGCCGAAAAACTGGGCAAGGTGTTCCTTCCCCGCCAGGCAATCGACCTGGTCACGGCAGAGCTGCAATAA

Upstream 100 bases:

>100_bases
CGCTGCCGCAGGCCAGGCATGGCGCCACAAACGTTTCAAAGACGAAGAGCCATTGCACATGATATGGTGATGCCACTTCT
GTTGTGGCAGGGGAGGAACC

Downstream 100 bases:

>100_bases
CTCGAATCCGGCCGCGGTGAAACAGCGGATGACTTTCCGCGTGCCCCCCCTTGAAAAATAACCACCCGCTTATTATGTTG
TAGCGTGCCATTTCCATTCG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MGETTVVTALIAIGFALSACACGSPGALSGNRDAGQVKNVIFLLSDGTANEAWPLARWVKGKRLASDAILSGAIRTYGAD
SIITDSAPGSTSYATGQKGSDKGIAVYPWNVTIAGVDCDPAMAYVPLATVLEGAKLTGRATGVVATSNVQHASPADFTAH
THDRSNYLEIGEQQVYQDMDVVLSGGEQYLLPKGVGGGVRTDNENLVQVVKAKGYAYVTNRDQMLAASGQKIFGLFAKDA
MAYDIDRATTAPGEPSLAEMTTKALETLSSSSKGKDRGFFLFVEGSKIDWAAHDNDPAGVVSDLIAYDDAVKAALDFAEA
DGTTLVISVADHTTGGLSIGVRSDPHYSTTDDDFVVAPMRKARLTAEGLGRRIEKSNSEAAIRAAFADSWGITDLSADEI
ASIRKSRPGKALQKVMGSMLSRRARLGWTTGGHTGGDPYLFSYGPGRISGLWENSALGTYVAGKLGFTFPEINKRLFVEA
SAAFRAEGFSTSIDLSDATNPVLTVSKGTATARLPLAKNLVLVNGKTIELEGIVVMAEKLGKVFLPRQAIDLVTAELQ

Sequences:

>Translated_558_residues
MGETTVVTALIAIGFALSACACGSPGALSGNRDAGQVKNVIFLLSDGTANEAWPLARWVKGKRLASDAILSGAIRTYGAD
SIITDSAPGSTSYATGQKGSDKGIAVYPWNVTIAGVDCDPAMAYVPLATVLEGAKLTGRATGVVATSNVQHASPADFTAH
THDRSNYLEIGEQQVYQDMDVVLSGGEQYLLPKGVGGGVRTDNENLVQVVKAKGYAYVTNRDQMLAASGQKIFGLFAKDA
MAYDIDRATTAPGEPSLAEMTTKALETLSSSSKGKDRGFFLFVEGSKIDWAAHDNDPAGVVSDLIAYDDAVKAALDFAEA
DGTTLVISVADHTTGGLSIGVRSDPHYSTTDDDFVVAPMRKARLTAEGLGRRIEKSNSEAAIRAAFADSWGITDLSADEI
ASIRKSRPGKALQKVMGSMLSRRARLGWTTGGHTGGDPYLFSYGPGRISGLWENSALGTYVAGKLGFTFPEINKRLFVEA
SAAFRAEGFSTSIDLSDATNPVLTVSKGTATARLPLAKNLVLVNGKTIELEGIVVMAEKLGKVFLPRQAIDLVTAELQ
>Mature_557_residues
GETTVVTALIAIGFALSACACGSPGALSGNRDAGQVKNVIFLLSDGTANEAWPLARWVKGKRLASDAILSGAIRTYGADS
IITDSAPGSTSYATGQKGSDKGIAVYPWNVTIAGVDCDPAMAYVPLATVLEGAKLTGRATGVVATSNVQHASPADFTAHT
HDRSNYLEIGEQQVYQDMDVVLSGGEQYLLPKGVGGGVRTDNENLVQVVKAKGYAYVTNRDQMLAASGQKIFGLFAKDAM
AYDIDRATTAPGEPSLAEMTTKALETLSSSSKGKDRGFFLFVEGSKIDWAAHDNDPAGVVSDLIAYDDAVKAALDFAEAD
GTTLVISVADHTTGGLSIGVRSDPHYSTTDDDFVVAPMRKARLTAEGLGRRIEKSNSEAAIRAAFADSWGITDLSADEIA
SIRKSRPGKALQKVMGSMLSRRARLGWTTGGHTGGDPYLFSYGPGRISGLWENSALGTYVAGKLGFTFPEINKRLFVEAS
AAFRAEGFSTSIDLSDATNPVLTVSKGTATARLPLAKNLVLVNGKTIELEGIVVMAEKLGKVFLPRQAIDLVTAELQ

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=337, Percent_Identity=33.8278931750742, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI94721246, Length=337, Percent_Identity=33.2344213649852, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157266292, Length=338, Percent_Identity=32.8402366863905, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI116734717, Length=340, Percent_Identity=28.2352941176471, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI294660770, Length=336, Percent_Identity=27.3809523809524, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI294660772, Length=292, Percent_Identity=27.7397260273973, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI48994877, Length=352, Percent_Identity=31.8181818181818, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320689, Length=518, Percent_Identity=30.1158301158301, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI85725200, Length=331, Percent_Identity=32.3262839879154, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI85815833, Length=331, Percent_Identity=32.3262839879154, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355151, Length=335, Percent_Identity=30.7462686567164, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355981, Length=336, Percent_Identity=30.0595238095238, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24585213, Length=361, Percent_Identity=29.3628808864266, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19921912, Length=340, Percent_Identity=30.2941176470588, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24657827, Length=346, Percent_Identity=29.7687861271676, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24657842, Length=343, Percent_Identity=27.1137026239067, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24657835, Length=341, Percent_Identity=28.7390029325513, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859923, Length=320, Percent_Identity=28.75, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21358067, Length=346, Percent_Identity=28.6127167630058, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24651554, Length=351, Percent_Identity=27.6353276353276, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24651556, Length=305, Percent_Identity=28.8524590163934, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355149, Length=349, Percent_Identity=27.7936962750716, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 58695; Mature: 58564

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETTVVTALIAIGFALSACACGSPGALSGNRDAGQVKNVIFLLSDGTANEAWPLARWVK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHC
GKRLASDAILSGAIRTYGADSIITDSAPGSTSYATGQKGSDKGIAVYPWNVTIAGVDCDP
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCC
AMAYVPLATVLEGAKLTGRATGVVATSNVQHASPADFTAHTHDRSNYLEIGEQQVYQDMD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHCHHHHHHHHH
VVLSGGEQYLLPKGVGGGVRTDNENLVQVVKAKGYAYVTNRDQMLAASGQKIFGLFAKDA
HEECCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCEEEEHHHHH
MAYDIDRATTAPGEPSLAEMTTKALETLSSSSKGKDRGFFLFVEGSKIDWAAHDNDPAGV
HEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEECCCCCCHHH
VSDLIAYDDAVKAALDFAEADGTTLVISVADHTTGGLSIGVRSDPHYSTTDDDFVVAPMR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCH
KARLTAEGLGRRIEKSNSEAAIRAAFADSWGITDLSADEIASIRKSRPGKALQKVMGSML
HHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
SRRARLGWTTGGHTGGDPYLFSYGPGRISGLWENSALGTYVAGKLGFTFPEINKRLFVEA
HHHHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEE
SAAFRAEGFSTSIDLSDATNPVLTVSKGTATARLPLAKNLVLVNGKTIELEGIVVMAEKL
CCHHCCCCCCCEEECCCCCCCEEEEECCCCEEECCCCCCEEEECCCEEEEEEEEEEEHHH
GKVFLPRQAIDLVTAELQ
CHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
GETTVVTALIAIGFALSACACGSPGALSGNRDAGQVKNVIFLLSDGTANEAWPLARWVK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHC
GKRLASDAILSGAIRTYGADSIITDSAPGSTSYATGQKGSDKGIAVYPWNVTIAGVDCDP
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCC
AMAYVPLATVLEGAKLTGRATGVVATSNVQHASPADFTAHTHDRSNYLEIGEQQVYQDMD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHCHHHHHHHHH
VVLSGGEQYLLPKGVGGGVRTDNENLVQVVKAKGYAYVTNRDQMLAASGQKIFGLFAKDA
HEECCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCEEEEHHHHH
MAYDIDRATTAPGEPSLAEMTTKALETLSSSSKGKDRGFFLFVEGSKIDWAAHDNDPAGV
HEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEEECCCCCCHHH
VSDLIAYDDAVKAALDFAEADGTTLVISVADHTTGGLSIGVRSDPHYSTTDDDFVVAPMR
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCH
KARLTAEGLGRRIEKSNSEAAIRAAFADSWGITDLSADEIASIRKSRPGKALQKVMGSML
HHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
SRRARLGWTTGGHTGGDPYLFSYGPGRISGLWENSALGTYVAGKLGFTFPEINKRLFVEA
HHHHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEE
SAAFRAEGFSTSIDLSDATNPVLTVSKGTATARLPLAKNLVLVNGKTIELEGIVVMAEKL
CCHHCCCCCCCEEECCCCCCCEEEEECCCCEEECCCCCCEEEECCCEEEEEEEEEEEHHH
GKVFLPRQAIDLVTAELQ
CHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]