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The map label for this gene is folC [H]

Identifier: 118579591

GI number: 118579591

Start: 1210377

End: 1211654

Strand: Reverse

Name: folC [H]

Synonym: Ppro_1160

Alternate gene names: 118579591

Gene position: 1211654-1210377 (Counterclockwise)

Preceding gene: 118579592

Following gene: 118579590

Centisome position: 30.23

GC content: 68.39

Gene sequence:

>1278_bases
ATGCCCCTGGCCGAAACCCTGGAGAGGCTCTACGCCCGCCGCAGGTTCGGCATCCGGCCCGGCATCCAGAGGGTCCGCAC
CCTGCTGGACCGGCTGGGCAACCCCGAGCAGGCTTTTCGCAGCATCCACGTCGTCGGCACCAACGGCAAGGGCTCCACAG
CCGCCTTTCTCTCGTCCATACTGACAGCGGCCGGCCACCGCAGCGCCCTTTTCACCTCACCCCACCTGATCCACTTCAGG
GAGCGCTTCCGCGTCAATGGCCGGGAACCGGGGGATGAACTGCTGGCCGCGCATCTGGAGCGGGTGCTCTCCCGGGCGCC
CGACGACGCCACCTTTTTCGAGATCGTCACCGCCCTGGCGGCCCTGCTGTTCGCCGAAGAGCGGATCGAGCTGGCCGTAA
TGGAGGCAGGCATGGGAGGTGGCTCCGATGCCACGGCAGCCCTGGCCGGCATGATGACGCTCATGACCCCCATCTCCCTG
GACCACTGCGACTACCTGGGTGGCAGCCTGGAGAGGATCGCCGCCGAGAAGAGCGCCATCATCGAACCGGGCACGCCGGT
GCTCGTCTCCCGCCAGACAACAACGGTGGGCGACACTATCCGCTCCTTCTGCCGCTGCGGCCAATCCCCCCTCTTCCAGT
GGGGAACGGACTTCAGCGCCAGCTGGAGCGGGGCCGGACGCCTGGACTACCGCGGCATGGGCGGCGTTCAGCAGGGGCTT
GCCCCCGGCATCCCGGGACGCTACCAGGCGGAGAACGCTTCACTGGCCCTGGCCGCCGCCGAGACGTTGGACGGGATGGG
CATAACGGTACCGGCCAGTGCCATGGCCTCCGGCCTGGCGCAGGCAAGTTGGCCGGGACGCATGGAGCTGATCCCCGGCC
CTCCCCGGCTGCTGCTGGACGGGGCCCACAACCAGGCCGGCGCCCAGGCCCTGGCGGAGGCACTGGGAGACTATTGCTAC
GACCGGCTGCTGCTGGTGGCGGGGGTCATGGCCGACAAGGACGTGCATGCCATCATGGCCCCCCTGGCGCCGCTGGTGAC
CAGAGGCTACTGCGTCACCCCGTCCATGGAGCGCGCCCTGGACGATACGGAACTGGCACGGCTCCTGGCGGGACTGGGCG
TGGAGGCACTTGCCTCCGGCGGCGTGGCCGACGGAATCGCGGCGGCACGGAACGAGGCCCACAGCCAAGACCTGATCCTG
ATATGCGGTTCCCTGTTCACCGTGGGTGAAGCTATGGCCGCATTGACGGGACACAGATTTGAAGGAATCCGGGGATAG

Upstream 100 bases:

>100_bases
CTGCTGGAGCACGGCATGCTGGACGTGATCGTCCCCCGCACGGAGATGCGCGCCAAACTGGCCTCGATCCTCGGCATACT
GTACCGCCCCGCTGCCTGAG

Downstream 100 bases:

>100_bases
GATCGTCCAACTCATGAAAATCAAACGTTTACTGATACTACTCGCCTGCCTGCTGCTTCACGCGGGAACCTGCCACGCCG
AAGATGACATAACCATCAGG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MPLAETLERLYARRRFGIRPGIQRVRTLLDRLGNPEQAFRSIHVVGTNGKGSTAAFLSSILTAAGHRSALFTSPHLIHFR
ERFRVNGREPGDELLAAHLERVLSRAPDDATFFEIVTALAALLFAEERIELAVMEAGMGGGSDATAALAGMMTLMTPISL
DHCDYLGGSLERIAAEKSAIIEPGTPVLVSRQTTTVGDTIRSFCRCGQSPLFQWGTDFSASWSGAGRLDYRGMGGVQQGL
APGIPGRYQAENASLALAAAETLDGMGITVPASAMASGLAQASWPGRMELIPGPPRLLLDGAHNQAGAQALAEALGDYCY
DRLLLVAGVMADKDVHAIMAPLAPLVTRGYCVTPSMERALDDTELARLLAGLGVEALASGGVADGIAAARNEAHSQDLIL
ICGSLFTVGEAMAALTGHRFEGIRG

Sequences:

>Translated_425_residues
MPLAETLERLYARRRFGIRPGIQRVRTLLDRLGNPEQAFRSIHVVGTNGKGSTAAFLSSILTAAGHRSALFTSPHLIHFR
ERFRVNGREPGDELLAAHLERVLSRAPDDATFFEIVTALAALLFAEERIELAVMEAGMGGGSDATAALAGMMTLMTPISL
DHCDYLGGSLERIAAEKSAIIEPGTPVLVSRQTTTVGDTIRSFCRCGQSPLFQWGTDFSASWSGAGRLDYRGMGGVQQGL
APGIPGRYQAENASLALAAAETLDGMGITVPASAMASGLAQASWPGRMELIPGPPRLLLDGAHNQAGAQALAEALGDYCY
DRLLLVAGVMADKDVHAIMAPLAPLVTRGYCVTPSMERALDDTELARLLAGLGVEALASGGVADGIAAARNEAHSQDLIL
ICGSLFTVGEAMAALTGHRFEGIRG
>Mature_424_residues
PLAETLERLYARRRFGIRPGIQRVRTLLDRLGNPEQAFRSIHVVGTNGKGSTAAFLSSILTAAGHRSALFTSPHLIHFRE
RFRVNGREPGDELLAAHLERVLSRAPDDATFFEIVTALAALLFAEERIELAVMEAGMGGGSDATAALAGMMTLMTPISLD
HCDYLGGSLERIAAEKSAIIEPGTPVLVSRQTTTVGDTIRSFCRCGQSPLFQWGTDFSASWSGAGRLDYRGMGGVQQGLA
PGIPGRYQAENASLALAAAETLDGMGITVPASAMASGLAQASWPGRMELIPGPPRLLLDGAHNQAGAQALAEALGDYCYD
RLLLVAGVMADKDVHAIMAPLAPLVTRGYCVTPSMERALDDTELARLLAGLGVEALASGGVADGIAAARNEAHSQDLILI
CGSLFTVGEAMAALTGHRFEGIRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=315, Percent_Identity=30.7936507936508, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI66932984, Length=315, Percent_Identity=30.7936507936508, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788654, Length=429, Percent_Identity=30.7692307692308, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71984923, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17553150, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=28.110599078341, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=350, Percent_Identity=27.4285714285714, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322718, Length=283, Percent_Identity=22.2614840989399, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641571, Length=297, Percent_Identity=28.6195286195286, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24581568, Length=171, Percent_Identity=29.2397660818713, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44790; Mature: 44659

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAETLERLYARRRFGIRPGIQRVRTLLDRLGNPEQAFRSIHVVGTNGKGSTAAFLSSI
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCEEEEEECCCCCHHHHHHHHH
LTAAGHRSALFTSPHLIHFRERFRVNGREPGDELLAAHLERVLSRAPDDATFFEIVTALA
HHHCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALLFAEERIELAVMEAGMGGGSDATAALAGMMTLMTPISLDHCDYLGGSLERIAAEKSAI
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHC
IEPGTPVLVSRQTTTVGDTIRSFCRCGQSPLFQWGTDFSASWSGAGRLDYRGMGGVQQGL
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
APGIPGRYQAENASLALAAAETLDGMGITVPASAMASGLAQASWPGRMELIPGPPRLLLD
CCCCCCCEECCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEE
GAHNQAGAQALAEALGDYCYDRLLLVAGVMADKDVHAIMAPLAPLVTRGYCVTPSMERAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCHHHHHC
DDTELARLLAGLGVEALASGGVADGIAAARNEAHSQDLILICGSLFTVGEAMAALTGHRF
CHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EGIRG
CCCCC
>Mature Secondary Structure 
PLAETLERLYARRRFGIRPGIQRVRTLLDRLGNPEQAFRSIHVVGTNGKGSTAAFLSSI
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCEEEEEECCCCCHHHHHHHHH
LTAAGHRSALFTSPHLIHFRERFRVNGREPGDELLAAHLERVLSRAPDDATFFEIVTALA
HHHCCCCCEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALLFAEERIELAVMEAGMGGGSDATAALAGMMTLMTPISLDHCDYLGGSLERIAAEKSAI
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHC
IEPGTPVLVSRQTTTVGDTIRSFCRCGQSPLFQWGTDFSASWSGAGRLDYRGMGGVQQGL
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
APGIPGRYQAENASLALAAAETLDGMGITVPASAMASGLAQASWPGRMELIPGPPRLLLD
CCCCCCCEECCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEE
GAHNQAGAQALAEALGDYCYDRLLLVAGVMADKDVHAIMAPLAPLVTRGYCVTPSMERAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCHHHHHC
DDTELARLLAGLGVEALASGGVADGIAAARNEAHSQDLILICGSLFTVGEAMAALTGHRF
CHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EGIRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]