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The map label for this gene is lptD [H]

Identifier: 118579590

GI number: 118579590

Start: 1208303

End: 1210363

Strand: Reverse

Name: lptD [H]

Synonym: Ppro_1159

Alternate gene names: 118579590

Gene position: 1210363-1208303 (Counterclockwise)

Preceding gene: 118579591

Following gene: 118579589

Centisome position: 30.2

GC content: 61.52

Gene sequence:

>2061_bases
ATGAAAATCAAACGTTTACTGATACTACTCGCCTGCCTGCTGCTTCACGCGGGAACCTGCCACGCCGAAGATGACATAAC
CATCAGGGCCGACGCCATGACCCACAACCAGGCCGATGACAGCGTCTCGGCCGACGGCAACGTCAGGATGGAGTGGGGCG
GCATGACCATGACCGCCCGCCGCGCCACCTACAACCGCGAGACCAGGATACTCACCGCCTACGAGGATGTGGTCATCACC
AAGGGCGAGGACACCCTGCGGGGCGACAAGGCGACCTGGGACATGGCCAGCGGACGGGGCGAACTGAACCACGCTGTCGC
CGGCGTGGCCGACTCCAACATGACGCTCACTGGCGACAGAATCGTCCGCAACGACGACGGCACCCTCACCCTCAGTAACA
CCGAGATGACCACCTGCGAACTCCCCGACCCCAGCTGGAAGTTTTCCGCCGAACATTTGGAGGTGAATCCGAAGGGGTAT
GCCATCGGCAGGAACCTGGTCTTCTACATCAGGGACACCCCGGTCATGTACGTCCCCTGGATGGCTTTTCCCGCCTCGGG
GGAGAAGAAGTCCGGCCTGCTCTTCCCCCGCGTCGGTTATTCCACGAAGCGCGGGGCGCAACTGGACATCCCCCTCTACT
GGGTCATCTCCCCCAGCCAGGACGCCACCTTCGACCTGGACATGATGAGCAAACGGGGAGTCGGCCTGGGAGTGGATTAC
CGCTACCTGCGCAAGCGCGGCAGCGAAGGCACCATGTACGGCTACCTGATCTACGACACCAAGGATAGTCGCTGGCGCGG
CCAAATAGCCCAGACGCACCGCGAGATCTTCTCGCCGGACATGAACCTGCGCACCTCCATCAACCTGACCACCGACCGCG
ATTTCCTGGATGATTTCGGCGTCAAGAGCGGCGACTACAACCGCCAGTACAACGCCACCATCGTCAATGCCCTCAAGACC
TGGCAGCATTACGCCCTGAACGCCACTCTGCGCTACAGCGAGGACATGTACGCGGAGAACAACCGCGACACCCTGCAGAC
CCTCCCCTCCATCGGCCTGTCTGCGGTGCGCCAGCAGATCTTCAATACCCCGCTCTACTTCGACCTGGACGCGACAGCAA
CGAACTTCTACCGCGACAACGGCGTCAGCGGTGAGCGTCTGCACGCTTTTCCCCGCCTGAGCATGGTTACCGGACTGCCC
GGCTACCTGAACGCCACCGTCTACGCCGGCCTGCACCTGCGCGGATACAACGGCACGGACATCCCCAGTAACAGCGACGT
CAAGGACACCACCGGCAACCTGATGCCCGAGGTCGGAGCAACCATCTCCAGTTCCTTCAGCAGGGTCTACGAACTGGACG
GCGAGAGCCTGAAGAAGCTGCGCCACGAACTGACCCCGGAAGTGTCCTACCGCTACGCGCCCCAGCGGGACCAGTCGCGA
CTCCCCTTCTACGACTACGAGGATCGGCTCCTGCACCAGAACGTGATCTACTACGGGGTGACCAGCTACCTGACCGGGAA
GTTCCAGAACGGGGAGAGCAGCGAGTATCGCGATATTTCCCGCATCAAACTGATGCAGGGGTACAGCTTCGACGGCAGCC
GCAGGGACGAACTGTCCATGGTGGATAACAGCCACGACGCATCAGACCTGATCCTGGAGAGCGAAACCTGGCTGCATCCA
CAGGCCAAACTAACCTTCGACGCACGGTACGACCTCCATCACAACTACCTCTCCAGCGCCCAACCCGGCCTGGAATACGA
CAGCAAGCGGGGCAGCAGCGCCGCCATCAGCTACCGCATGACCCGCAACAGCGAAAACAGCGCCAACCAGGTTGAATACC
TGGAGGCCAGGCTGGCCACAAAACGCTTCAAACCGTGGAACTTCGGCTACACCACCCGCTACTCCTTCGACAAATCCGGG
TTCCTGGAATCGGTTTACAGCGTGGAGTACCAGCACCAGTGCTGGAGCGTGGAACTGGCCGTTCACCACCGTTCCGGCAA
TCCGTCCTTCACCATCGGCTTCAATCTGGCCGGCCTGGCGGACTACGCCTCGCGCCGCTAG

Upstream 100 bases:

>100_bases
CTGATCCTGATATGCGGTTCCCTGTTCACCGTGGGTGAAGCTATGGCCGCATTGACGGGACACAGATTTGAAGGAATCCG
GGGATAGGATCGTCCAACTC

Downstream 100 bases:

>100_bases
ACTCCCCCCTCCAGCACAATATCCCCCACAGGTTGTCCACTCCCGCGCCTCCCCGCTCCGACGGGCAGGCGCGGACAAGT
TTTAACTATACACAACGCCA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MKIKRLLILLACLLLHAGTCHAEDDITIRADAMTHNQADDSVSADGNVRMEWGGMTMTARRATYNRETRILTAYEDVVIT
KGEDTLRGDKATWDMASGRGELNHAVAGVADSNMTLTGDRIVRNDDGTLTLSNTEMTTCELPDPSWKFSAEHLEVNPKGY
AIGRNLVFYIRDTPVMYVPWMAFPASGEKKSGLLFPRVGYSTKRGAQLDIPLYWVISPSQDATFDLDMMSKRGVGLGVDY
RYLRKRGSEGTMYGYLIYDTKDSRWRGQIAQTHREIFSPDMNLRTSINLTTDRDFLDDFGVKSGDYNRQYNATIVNALKT
WQHYALNATLRYSEDMYAENNRDTLQTLPSIGLSAVRQQIFNTPLYFDLDATATNFYRDNGVSGERLHAFPRLSMVTGLP
GYLNATVYAGLHLRGYNGTDIPSNSDVKDTTGNLMPEVGATISSSFSRVYELDGESLKKLRHELTPEVSYRYAPQRDQSR
LPFYDYEDRLLHQNVIYYGVTSYLTGKFQNGESSEYRDISRIKLMQGYSFDGSRRDELSMVDNSHDASDLILESETWLHP
QAKLTFDARYDLHHNYLSSAQPGLEYDSKRGSSAAISYRMTRNSENSANQVEYLEARLATKRFKPWNFGYTTRYSFDKSG
FLESVYSVEYQHQCWSVELAVHHRSGNPSFTIGFNLAGLADYASRR

Sequences:

>Translated_686_residues
MKIKRLLILLACLLLHAGTCHAEDDITIRADAMTHNQADDSVSADGNVRMEWGGMTMTARRATYNRETRILTAYEDVVIT
KGEDTLRGDKATWDMASGRGELNHAVAGVADSNMTLTGDRIVRNDDGTLTLSNTEMTTCELPDPSWKFSAEHLEVNPKGY
AIGRNLVFYIRDTPVMYVPWMAFPASGEKKSGLLFPRVGYSTKRGAQLDIPLYWVISPSQDATFDLDMMSKRGVGLGVDY
RYLRKRGSEGTMYGYLIYDTKDSRWRGQIAQTHREIFSPDMNLRTSINLTTDRDFLDDFGVKSGDYNRQYNATIVNALKT
WQHYALNATLRYSEDMYAENNRDTLQTLPSIGLSAVRQQIFNTPLYFDLDATATNFYRDNGVSGERLHAFPRLSMVTGLP
GYLNATVYAGLHLRGYNGTDIPSNSDVKDTTGNLMPEVGATISSSFSRVYELDGESLKKLRHELTPEVSYRYAPQRDQSR
LPFYDYEDRLLHQNVIYYGVTSYLTGKFQNGESSEYRDISRIKLMQGYSFDGSRRDELSMVDNSHDASDLILESETWLHP
QAKLTFDARYDLHHNYLSSAQPGLEYDSKRGSSAAISYRMTRNSENSANQVEYLEARLATKRFKPWNFGYTTRYSFDKSG
FLESVYSVEYQHQCWSVELAVHHRSGNPSFTIGFNLAGLADYASRR
>Mature_686_residues
MKIKRLLILLACLLLHAGTCHAEDDITIRADAMTHNQADDSVSADGNVRMEWGGMTMTARRATYNRETRILTAYEDVVIT
KGEDTLRGDKATWDMASGRGELNHAVAGVADSNMTLTGDRIVRNDDGTLTLSNTEMTTCELPDPSWKFSAEHLEVNPKGY
AIGRNLVFYIRDTPVMYVPWMAFPASGEKKSGLLFPRVGYSTKRGAQLDIPLYWVISPSQDATFDLDMMSKRGVGLGVDY
RYLRKRGSEGTMYGYLIYDTKDSRWRGQIAQTHREIFSPDMNLRTSINLTTDRDFLDDFGVKSGDYNRQYNATIVNALKT
WQHYALNATLRYSEDMYAENNRDTLQTLPSIGLSAVRQQIFNTPLYFDLDATATNFYRDNGVSGERLHAFPRLSMVTGLP
GYLNATVYAGLHLRGYNGTDIPSNSDVKDTTGNLMPEVGATISSSFSRVYELDGESLKKLRHELTPEVSYRYAPQRDQSR
LPFYDYEDRLLHQNVIYYGVTSYLTGKFQNGESSEYRDISRIKLMQGYSFDGSRRDELSMVDNSHDASDLILESETWLHP
QAKLTFDARYDLHHNYLSSAQPGLEYDSKRGSSAAISYRMTRNSENSANQVEYLEARLATKRFKPWNFGYTTRYSFDKSG
FLESVYSVEYQHQCWSVELAVHHRSGNPSFTIGFNLAGLADYASRR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=506, Percent_Identity=22.9249011857708, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 77856; Mature: 77856

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRLLILLACLLLHAGTCHAEDDITIRADAMTHNQADDSVSADGNVRMEWGGMTMTAR
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEE
RATYNRETRILTAYEDVVITKGEDTLRGDKATWDMASGRGELNHAVAGVADSNMTLTGDR
CCCCCCCEEEEEEECCEEEECCCCCCCCCCCEEECCCCCCCCCHHEEEECCCCEEEECCE
IVRNDDGTLTLSNTEMTTCELPDPSWKFSAEHLEVNPKGYAIGRNLVFYIRDTPVMYVPW
EEECCCCEEEECCCEEEEEECCCCCCEEECCEEEECCCCEEECCEEEEEEECCCEEEEEE
MAFPASGEKKSGLLFPRVGYSTKRGAQLDIPLYWVISPSQDATFDLDMMSKRGVGLGVDY
EEECCCCCCCCCEEEECCCCCCCCCCEECCEEEEEEECCCCCEEEHHHHHCCCCCCCCCH
RYLRKRGSEGTMYGYLIYDTKDSRWRGQIAQTHREIFSPDMNLRTSINLTTDRDFLDDFG
HHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCC
VKSGDYNRQYNATIVNALKTWQHYALNATLRYSEDMYAENNRDTLQTLPSIGLSAVRQQI
CCCCCCCCEECHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHH
FNTPLYFDLDATATNFYRDNGVSGERLHAFPRLSMVTGLPGYLNATVYAGLHLRGYNGTD
CCCCEEEEECCCEEEEECCCCCCCCCEECCCCHHHHHCCCCHHCEEEEEEEEEECCCCCC
IPSNSDVKDTTGNLMPEVGATISSSFSRVYELDGESLKKLRHELTPEVSYRYAPQRDQSR
CCCCCCCCCCCCCCCCHHCHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCCCCCCC
LPFYDYEDRLLHQNVIYYGVTSYLTGKFQNGESSEYRDISRIKLMQGYSFDGSRRDELSM
CCCCCCCHHHHHCCEEEEEHHHHEEEECCCCCCCCHHHHHHEEEEECCCCCCCCCCCHHC
VDNSHDASDLILESETWLHPQAKLTFDARYDLHHNYLSSAQPGLEYDSKRGSSAAISYRM
CCCCCCCCCEEECCCCCCCCCEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
TRNSENSANQVEYLEARLATKRFKPWNFGYTTRYSFDKSGFLESVYSVEYQHQCWSVELA
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEEEEE
VHHRSGNPSFTIGFNLAGLADYASRR
EEECCCCCCEEEEEEECCHHHHHCCC
>Mature Secondary Structure
MKIKRLLILLACLLLHAGTCHAEDDITIRADAMTHNQADDSVSADGNVRMEWGGMTMTAR
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEE
RATYNRETRILTAYEDVVITKGEDTLRGDKATWDMASGRGELNHAVAGVADSNMTLTGDR
CCCCCCCEEEEEEECCEEEECCCCCCCCCCCEEECCCCCCCCCHHEEEECCCCEEEECCE
IVRNDDGTLTLSNTEMTTCELPDPSWKFSAEHLEVNPKGYAIGRNLVFYIRDTPVMYVPW
EEECCCCEEEECCCEEEEEECCCCCCEEECCEEEECCCCEEECCEEEEEEECCCEEEEEE
MAFPASGEKKSGLLFPRVGYSTKRGAQLDIPLYWVISPSQDATFDLDMMSKRGVGLGVDY
EEECCCCCCCCCEEEECCCCCCCCCCEECCEEEEEEECCCCCEEEHHHHHCCCCCCCCCH
RYLRKRGSEGTMYGYLIYDTKDSRWRGQIAQTHREIFSPDMNLRTSINLTTDRDFLDDFG
HHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCC
VKSGDYNRQYNATIVNALKTWQHYALNATLRYSEDMYAENNRDTLQTLPSIGLSAVRQQI
CCCCCCCCEECHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHH
FNTPLYFDLDATATNFYRDNGVSGERLHAFPRLSMVTGLPGYLNATVYAGLHLRGYNGTD
CCCCEEEEECCCEEEEECCCCCCCCCEECCCCHHHHHCCCCHHCEEEEEEEEEECCCCCC
IPSNSDVKDTTGNLMPEVGATISSSFSRVYELDGESLKKLRHELTPEVSYRYAPQRDQSR
CCCCCCCCCCCCCCCCHHCHHHHHHHHHHEECCCHHHHHHHHHCCCCCEEEECCCCCCCC
LPFYDYEDRLLHQNVIYYGVTSYLTGKFQNGESSEYRDISRIKLMQGYSFDGSRRDELSM
CCCCCCCHHHHHCCEEEEEHHHHEEEECCCCCCCCHHHHHHEEEEECCCCCCCCCCCHHC
VDNSHDASDLILESETWLHPQAKLTFDARYDLHHNYLSSAQPGLEYDSKRGSSAAISYRM
CCCCCCCCCEEECCCCCCCCCEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
TRNSENSANQVEYLEARLATKRFKPWNFGYTTRYSFDKSGFLESVYSVEYQHQCWSVELA
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEEEEE
VHHRSGNPSFTIGFNLAGLADYASRR
EEECCCCCCEEEEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA