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The map label for this gene is acoC [H]

Identifier: 118579460

GI number: 118579460

Start: 1075772

End: 1077124

Strand: Direct

Name: acoC [H]

Synonym: Ppro_1026

Alternate gene names: 118579460

Gene position: 1075772-1077124 (Clockwise)

Preceding gene: 118579459

Following gene: 118579461

Centisome position: 26.84

GC content: 62.45

Gene sequence:

>1353_bases
ATGCCCAAATGGGGGCTGACCATGGAAGAGGGCACCCTGGCCAAGTGGCTGATCGACGAGGGAAGCCAGATCGAACCGGG
GATGGCTATTGCCGAGGTGGAGACCGACAAGATCGTCAACGTGATGGAGGCGAACCAGAGCGGTGTCCTGGCCAAGCAGA
TAGCCGATGAAGGCGATGTGCTGCCGGTGGGCGCGCTGATTGGCGTTATCACCCAAGGGGATGCCGCGGAAAGTGCCATC
GCCGATTTTATCGCCAATTTCGGCTCTGATGAGGAAGCAGCGCCAGCGGAAGAGTCTGCCTGCAGCGCAGCATCCGATGA
CATCTACTGCCTGACCATGCCCAAGTGGGGGCTGACCATGGAAGAGGGGACCCTGGTCAAGTGGTTGATCGATGAGGGAA
CCCAGATCGAGCTGGGCATGTCCATCGCCGAGGTGGAGACCGACAAGATCGTCAACGTGCTCGAAGCCACCCACGCCGGT
ATCCTGAGACGCAAGATCGCCGATGAAGGGGACGAACTCCCTGTTGGCGCTCTGCTGGGAGTGATCGCCGATGCGTCTGT
TTCCGACCAGGCCATTGACGCCTTCCTCGCCGGCGGGGCAACGGAGACGGCAGCGGAAGATGCCTCGGTGCCGGCAGCCG
AGGAACCGCAGGCTGAATCGGCAGCAGCGGGACAGATCCTGGACGGCTCGCTGCCGCTGGAGGGGATGCGCGCGGCCATA
TCCAAGACCGTGACCACCTCATGGACGACCATCCCGCACTATATGGTGACGGTGGCCATCGACATGGGCAGGGCGGAGGC
GCTGAGCAGCGCCCAGAAACAGGCCGGTAAGCGGGTCTCCATCAACGACATGCTGATCAAGGCATCGGCCCTGGCAATCG
GCAAGTACCCCCTGATCAACGCCGCCTTTAGCGGCAAGAACATCACGCTGCACGGAGACGTGAACGTCGCCATGGCCATC
GGTCTGGAAGAAGGAGTGATCATGCCGGTGATCAGGGAATGCCAGAAGCTCCCGGTCCAGCAAATCGGCGAGAGGAGCCG
CGAACTGGTGGCACTCGCCAAGGAGGGCAAGCTGGGCAGCGCCGAATTGAGCGGTGGGACCTTCGCCATATCCAACATGG
GGATGCTGGGGGTGGAGGATTTTATCGCCATCGTGCCGCCCAACCTGTCCGCTATCCTGGCCGTCGGCATGGTCAAGGAC
GAGCCGGTGGTGCGCGACGGCCAGGTGGTTGCTGCCCGCATGATGAGGGTGACCGTTTCCGCCGACCACCGGGTGCATGA
CGGCGCCTATGCCGCCAAATTCCTCGGTGAACTGAAGGGCATACTGGAAGCGCCGGAGACGATCCTGGCCTGA

Upstream 100 bases:

>100_bases
CGGTAAAGGCGATCATCTGAAAACATTCCAACGTCTTGATAGACACGATTCATAGGACAAGAAAGGTACGACAATGACTG
CATCAGTACAGGAATTAACC

Downstream 100 bases:

>100_bases
TAACGGGCCGGCACATGAATTGGATTACACGAAGTCGAAAGGAGAAGCTATGACGTCCCAGTTGAAGAAGGAAGATCTGC
TCAGGGCATACCGGACCATG

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPKWGLTMEEGTLAKWLIDEGSQIEPGMAIAEVETDKIVNVMEANQSGVLAKQIADEGDVLPVGALIGVITQGDAAESAI
ADFIANFGSDEEAAPAEESACSAASDDIYCLTMPKWGLTMEEGTLVKWLIDEGTQIELGMSIAEVETDKIVNVLEATHAG
ILRRKIADEGDELPVGALLGVIADASVSDQAIDAFLAGGATETAAEDASVPAAEEPQAESAAAGQILDGSLPLEGMRAAI
SKTVTTSWTTIPHYMVTVAIDMGRAEALSSAQKQAGKRVSINDMLIKASALAIGKYPLINAAFSGKNITLHGDVNVAMAI
GLEEGVIMPVIRECQKLPVQQIGERSRELVALAKEGKLGSAELSGGTFAISNMGMLGVEDFIAIVPPNLSAILAVGMVKD
EPVVRDGQVVAARMMRVTVSADHRVHDGAYAAKFLGELKGILEAPETILA

Sequences:

>Translated_450_residues
MPKWGLTMEEGTLAKWLIDEGSQIEPGMAIAEVETDKIVNVMEANQSGVLAKQIADEGDVLPVGALIGVITQGDAAESAI
ADFIANFGSDEEAAPAEESACSAASDDIYCLTMPKWGLTMEEGTLVKWLIDEGTQIELGMSIAEVETDKIVNVLEATHAG
ILRRKIADEGDELPVGALLGVIADASVSDQAIDAFLAGGATETAAEDASVPAAEEPQAESAAAGQILDGSLPLEGMRAAI
SKTVTTSWTTIPHYMVTVAIDMGRAEALSSAQKQAGKRVSINDMLIKASALAIGKYPLINAAFSGKNITLHGDVNVAMAI
GLEEGVIMPVIRECQKLPVQQIGERSRELVALAKEGKLGSAELSGGTFAISNMGMLGVEDFIAIVPPNLSAILAVGMVKD
EPVVRDGQVVAARMMRVTVSADHRVHDGAYAAKFLGELKGILEAPETILA
>Mature_449_residues
PKWGLTMEEGTLAKWLIDEGSQIEPGMAIAEVETDKIVNVMEANQSGVLAKQIADEGDVLPVGALIGVITQGDAAESAIA
DFIANFGSDEEAAPAEESACSAASDDIYCLTMPKWGLTMEEGTLVKWLIDEGTQIELGMSIAEVETDKIVNVLEATHAGI
LRRKIADEGDELPVGALLGVIADASVSDQAIDAFLAGGATETAAEDASVPAAEEPQAESAAAGQILDGSLPLEGMRAAIS
KTVTTSWTTIPHYMVTVAIDMGRAEALSSAQKQAGKRVSINDMLIKASALAIGKYPLINAAFSGKNITLHGDVNVAMAIG
LEEGVIMPVIRECQKLPVQQIGERSRELVALAKEGKLGSAELSGGTFAISNMGMLGVEDFIAIVPPNLSAILAVGMVKDE
PVVRDGQVVAARMMRVTVSADHRVHDGAYAAKFLGELKGILEAPETILA

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=278, Percent_Identity=34.1726618705036, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI203098816, Length=222, Percent_Identity=30.6306306306306, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI203098753, Length=222, Percent_Identity=30.6306306306306, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI110671329, Length=229, Percent_Identity=31.0043668122271, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=28.3400809716599, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=30.625, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=25.9259259259259, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1786305, Length=204, Percent_Identity=34.3137254901961, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17560088, Length=229, Percent_Identity=38.8646288209607, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=221, Percent_Identity=36.1990950226244, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17537937, Length=297, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=23.1143552311436, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=228, Percent_Identity=32.4561403508772, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=388, Percent_Identity=24.2268041237113, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=37.0044052863436, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=37.0044052863436, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI18859875, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645909, Length=202, Percent_Identity=30.1980198019802, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47001; Mature: 46870

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKWGLTMEEGTLAKWLIDEGSQIEPGMAIAEVETDKIVNVMEANQSGVLAKQIADEGDV
CCCCCCEECCCCEEEHHHCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHCCCCCE
LPVGALIGVITQGDAAESAIADFIANFGSDEEAAPAEESACSAASDDIYCLTMPKWGLTM
EEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEE
EEGTLVKWLIDEGTQIELGMSIAEVETDKIVNVLEATHAGILRRKIADEGDELPVGALLG
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VIADASVSDQAIDAFLAGGATETAAEDASVPAAEEPQAESAAAGQILDGSLPLEGMRAAI
HHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCHHHHHHHH
SKTVTTSWTTIPHYMVTVAIDMGRAEALSSAQKQAGKRVSINDMLIKASALAIGKYPLIN
HHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCEEEHHHHEEEHHHHEECCCCEEE
AAFSGKNITLHGDVNVAMAIGLEEGVIMPVIRECQKLPVQQIGERSRELVALAKEGKLGS
EEECCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCC
AELSGGTFAISNMGMLGVEDFIAIVPPNLSAILAVGMVKDEPVVRDGQVVAARMMRVTVS
EECCCCEEEECCCCCCCHHHHHEECCCCHHHHHEEECCCCCCCCCCCHHHHHHHHEEEEC
ADHRVHDGAYAAKFLGELKGILEAPETILA
CCCCCCCHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
PKWGLTMEEGTLAKWLIDEGSQIEPGMAIAEVETDKIVNVMEANQSGVLAKQIADEGDV
CCCCCEECCCCEEEHHHCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHCCCCCE
LPVGALIGVITQGDAAESAIADFIANFGSDEEAAPAEESACSAASDDIYCLTMPKWGLTM
EEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEE
EEGTLVKWLIDEGTQIELGMSIAEVETDKIVNVLEATHAGILRRKIADEGDELPVGALLG
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VIADASVSDQAIDAFLAGGATETAAEDASVPAAEEPQAESAAAGQILDGSLPLEGMRAAI
HHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCHHHHHHHH
SKTVTTSWTTIPHYMVTVAIDMGRAEALSSAQKQAGKRVSINDMLIKASALAIGKYPLIN
HHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCEEEHHHHEEEHHHHEECCCCEEE
AAFSGKNITLHGDVNVAMAIGLEEGVIMPVIRECQKLPVQQIGERSRELVALAKEGKLGS
EEECCCEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCC
AELSGGTFAISNMGMLGVEDFIAIVPPNLSAILAVGMVKDEPVVRDGQVVAARMMRVTVS
EECCCCEEEECCCCCCCHHHHHEECCCCHHHHHEEECCCCCCCCCCCHHHHHHHHEEEEC
ADHRVHDGAYAAKFLGELKGILEAPETILA
CCCCCCCHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]