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The map label for this gene is acoB [H]

Identifier: 118579459

GI number: 118579459

Start: 1074690

End: 1075691

Strand: Direct

Name: acoB [H]

Synonym: Ppro_1025

Alternate gene names: 118579459

Gene position: 1074690-1075691 (Clockwise)

Preceding gene: 118579458

Following gene: 118579460

Centisome position: 26.81

GC content: 62.48

Gene sequence:

>1002_bases
ATGTCGAGAAAACTCAATTATAAAGATGCGATCAACGAGGCTCTGTCCCAGGAAATGGCGCGTGACGAGAAGGTCATCGT
CATTGGTCTGGACGTTGCCGGCGGCCGGGGAACCCAGGGAGTCATGGATGCCTGGGGTGGTGTTCTGGGGGTAACCAAGG
GACTGTACGCGAAATACGGGGATCGTGTCATGGACACCCCCATCTCCGAATCCGCCTACATCGGCTCCGCCGTGGGCGCC
GCCGCCTGCGGCATGCGTCCGGTGGTGGAGATGATGTTCGCCGACTTCCTGGGGGTCTGTTTCGACCAGCTGATGAACCA
GGCGGCCAAATTCCGCTACATGTTCGGCGGCACGGCCGAGACGCCGGTTGTCTGTCGCATGATGTACGGCGGCGGTTTCC
GTGGCGCTGCCCAGCACTCCCAGTGCCTGTACTCCATCTTCGCCCACATCCCGGGACTGAAGGTCGTGCTTCCCTCCACG
CCTGCCGAGGCCAAGGGTCTCCTGATTCAGTCCATCCGTGACAACGACCCGGTCATCTTCATGGAGCACAAGGCGCTGTA
CGCCATGAAGGGCGAGGTCCCCGAGGAGAGCTACACCATCCCCTTCGGCCAGGCCAACATCGTCCGGGAGGGCAAAGACG
TGACCATCGTGGCGCTGGGCCGCATGGTGCACATGGCCACCCAGGCTGCGGCCTCCCTGGCCAAGGCCGGTATCGAGTGT
GAGGTGATCGACCCGCGCACCATCTCTCCGCTGGACACGGCCACGATCATCAAGAGTGTGGAGAAGACCGGCCGGCTGGT
GGTGGTGGACGAATCCCACCCGCGCGCCAGCATGGCCGGCGACATCTCCTCGGTGGTTGCCCAGGAAGCCTTCGGCTCCC
TGAAGGGAGCGATCAAGTTGGTGACCGCTCCGCACACTCCGGTACCGTTCAGCGATGTGCTGGAAGACCTCTACCTGCCC
AATGCCGCAAAAATCGAGGCAGCGGTAAAGGCGATCATCTGA

Upstream 100 bases:

>100_bases
AGACTCCGTGGTCAAGGCTCTGGCTGCGCCCAAACCAACGGCGGCGGATCTTCTGACCGGCGTCTACGTATCGTACTAGA
GAGCGGAAAGGAACCGATAT

Downstream 100 bases:

>100_bases
AAACATTCCAACGTCTTGATAGACACGATTCATAGGACAAGAAAGGTACGACAATGACTGCATCAGTACAGGAATTAACC
ATGCCCAAATGGGGGCTGAC

Product: transketolase, central region

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSRKLNYKDAINEALSQEMARDEKVIVIGLDVAGGRGTQGVMDAWGGVLGVTKGLYAKYGDRVMDTPISESAYIGSAVGA
AACGMRPVVEMMFADFLGVCFDQLMNQAAKFRYMFGGTAETPVVCRMMYGGGFRGAAQHSQCLYSIFAHIPGLKVVLPST
PAEAKGLLIQSIRDNDPVIFMEHKALYAMKGEVPEESYTIPFGQANIVREGKDVTIVALGRMVHMATQAAASLAKAGIEC
EVIDPRTISPLDTATIIKSVEKTGRLVVVDESHPRASMAGDISSVVAQEAFGSLKGAIKLVTAPHTPVPFSDVLEDLYLP
NAAKIEAAVKAII

Sequences:

>Translated_333_residues
MSRKLNYKDAINEALSQEMARDEKVIVIGLDVAGGRGTQGVMDAWGGVLGVTKGLYAKYGDRVMDTPISESAYIGSAVGA
AACGMRPVVEMMFADFLGVCFDQLMNQAAKFRYMFGGTAETPVVCRMMYGGGFRGAAQHSQCLYSIFAHIPGLKVVLPST
PAEAKGLLIQSIRDNDPVIFMEHKALYAMKGEVPEESYTIPFGQANIVREGKDVTIVALGRMVHMATQAAASLAKAGIEC
EVIDPRTISPLDTATIIKSVEKTGRLVVVDESHPRASMAGDISSVVAQEAFGSLKGAIKLVTAPHTPVPFSDVLEDLYLP
NAAKIEAAVKAII
>Mature_332_residues
SRKLNYKDAINEALSQEMARDEKVIVIGLDVAGGRGTQGVMDAWGGVLGVTKGLYAKYGDRVMDTPISESAYIGSAVGAA
ACGMRPVVEMMFADFLGVCFDQLMNQAAKFRYMFGGTAETPVVCRMMYGGGFRGAAQHSQCLYSIFAHIPGLKVVLPSTP
AEAKGLLIQSIRDNDPVIFMEHKALYAMKGEVPEESYTIPFGQANIVREGKDVTIVALGRMVHMATQAAASLAKAGIECE
VIDPRTISPLDTATIIKSVEKTGRLVVVDESHPRASMAGDISSVVAQEAFGSLKGAIKLVTAPHTPVPFSDVLEDLYLPN
AAKIEAAVKAII

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=336, Percent_Identity=40.4761904761905, Blast_Score=244, Evalue=5e-65,
Organism=Homo sapiens, GI4557353, Length=336, Percent_Identity=40.7738095238095, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI34101272, Length=336, Percent_Identity=40.7738095238095, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI291084858, Length=336, Percent_Identity=38.9880952380952, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI225637463, Length=296, Percent_Identity=27.7027027027027, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI225637461, Length=296, Percent_Identity=27.7027027027027, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI225637459, Length=296, Percent_Identity=27.7027027027027, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI133778974, Length=171, Percent_Identity=34.5029239766082, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786622, Length=293, Percent_Identity=25.2559726962457, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=332, Percent_Identity=38.855421686747, Blast_Score=226, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17506935, Length=332, Percent_Identity=40.3614457831325, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17539652, Length=294, Percent_Identity=29.9319727891156, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=339, Percent_Identity=38.6430678466077, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21358145, Length=338, Percent_Identity=39.6449704142012, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24650940, Length=338, Percent_Identity=39.6449704142012, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI160714828, Length=336, Percent_Identity=36.6071428571429, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI160714832, Length=336, Percent_Identity=36.6071428571429, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24650943, Length=96, Percent_Identity=42.7083333333333, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24650945, Length=96, Percent_Identity=42.7083333333333, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35506; Mature: 35374

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKLNYKDAINEALSQEMARDEKVIVIGLDVAGGRGTQGVMDAWGGVLGVTKGLYAKYG
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHCHHHHHHHHHHHHHC
DRVMDTPISESAYIGSAVGAAACGMRPVVEMMFADFLGVCFDQLMNQAAKFRYMFGGTAE
CHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVCRMMYGGGFRGAAQHSQCLYSIFAHIPGLKVVLPSTPAEAKGLLIQSIRDNDPVIF
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHEECCCCCCEEE
MEHKALYAMKGEVPEESYTIPFGQANIVREGKDVTIVALGRMVHMATQAAASLAKAGIEC
EECCEEEEECCCCCCCCEECCCCCCHHEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
EVIDPRTISPLDTATIIKSVEKTGRLVVVDESHPRASMAGDISSVVAQEAFGSLKGAIKL
EEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VTAPHTPVPFSDVLEDLYLPNAAKIEAAVKAII
EECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SRKLNYKDAINEALSQEMARDEKVIVIGLDVAGGRGTQGVMDAWGGVLGVTKGLYAKYG
CCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHCHHHHHHHHHHHHHC
DRVMDTPISESAYIGSAVGAAACGMRPVVEMMFADFLGVCFDQLMNQAAKFRYMFGGTAE
CHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVCRMMYGGGFRGAAQHSQCLYSIFAHIPGLKVVLPSTPAEAKGLLIQSIRDNDPVIF
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHEECCCCCCEEE
MEHKALYAMKGEVPEESYTIPFGQANIVREGKDVTIVALGRMVHMATQAAASLAKAGIEC
EECCEEEEECCCCCCCCEECCCCCCHHEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
EVIDPRTISPLDTATIIKSVEKTGRLVVVDESHPRASMAGDISSVVAQEAFGSLKGAIKL
EEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VTAPHTPVPFSDVLEDLYLPNAAKIEAAVKAII
EECCCCCCCHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]