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The map label for this gene is acoA [H]

Identifier: 118579461

GI number: 118579461

Start: 1077174

End: 1078148

Strand: Direct

Name: acoA [H]

Synonym: Ppro_1027

Alternate gene names: 118579461

Gene position: 1077174-1078148 (Clockwise)

Preceding gene: 118579460

Following gene: 118579462

Centisome position: 26.88

GC content: 62.56

Gene sequence:

>975_bases
ATGACGTCCCAGTTGAAGAAGGAAGATCTGCTCAGGGCATACCGGACCATGAGGGAGATCCGCGAGTTTGAAGACCGCCT
GCACATCGAGTTTGCCACGGGCAAGATTCCGGGGTTTGTGCACCTGTACTCGGGCGAGGAGGCTGTTGCCACGGGCGTGT
GCATGCACCTGACCGATGAGGACCGCATTTCCAGCACCCATCGCGGCCATGGCCACTGCATTGCCAAGGGTGTGGACATC
CACGGCATGATGGCCGAGATCTACGGCAAGAAGACCGGCACCTGCGGCGGCAAGGGTGGTTCCATGCACATCGCCGACCT
GGAGAAGGGGATGATGGGCGCCAACGGCATCGTGGGCGCCGGGCCTCCGCTGATCTGCGGCGCGGCCCTGGCAGCCAAGT
TCAAGGGTAATGGCGGGGTGGGTGTTGCTTTTTTCGGTGACGGCGCCTCCAATCAGGGGACCATCCTGGAGAGTATGAAC
CTGGCCTCGGTATGGAAGCTGCCGGTGATCTTCCTGAACGAGAACAACGGCTACGCCGAGTCAACCGGCGTCTCCTACAA
CGTTCCGACCCCCAACATAGCCGACCGCGCCGCCGGTTTCGGCATGCCGGGCGTCACCGTGGACGGCAACGACTTCTTCG
CCGTGTACGAGGCTGCCGGCGAGGCGATCCGCCGGGCCCGGGCGGGCGAGGGGCCGACGCTTCTGGAGTGCAAGACCCAG
CGTTACTTCGGCCACTTCGAAGGTGACGCCATGACCTACCGTCCCAACGGAGAGAGCCAGAACCTGCGCAAGACGAAAGA
CTGCCTGAAGATCTTTGCCGAGAAGGCCATTGCCGCCGGCCTGGTTACTGCCGCGGAACTGGAAGCCATTGACAAGGAAG
TTCTGGCCCTGATCGAAGACTCCGTGGTCAAGGCTCTGGCTGCGCCCAAACCAACGGCGGCGGATCTTCTGACCGGCGTC
TACGTATCGTACTAG

Upstream 100 bases:

>100_bases
CTCGGTGAACTGAAGGGCATACTGGAAGCGCCGGAGACGATCCTGGCCTGATAACGGGCCGGCACATGAATTGGATTACA
CGAAGTCGAAAGGAGAAGCT

Downstream 100 bases:

>100_bases
AGAGCGGAAAGGAACCGATATATGTCGAGAAAACTCAATTATAAAGATGCGATCAACGAGGCTCTGTCCCAGGAAATGGC
GCGTGACGAGAAGGTCATCG

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTSQLKKEDLLRAYRTMREIREFEDRLHIEFATGKIPGFVHLYSGEEAVATGVCMHLTDEDRISSTHRGHGHCIAKGVDI
HGMMAEIYGKKTGTCGGKGGSMHIADLEKGMMGANGIVGAGPPLICGAALAAKFKGNGGVGVAFFGDGASNQGTILESMN
LASVWKLPVIFLNENNGYAESTGVSYNVPTPNIADRAAGFGMPGVTVDGNDFFAVYEAAGEAIRRARAGEGPTLLECKTQ
RYFGHFEGDAMTYRPNGESQNLRKTKDCLKIFAEKAIAAGLVTAAELEAIDKEVLALIEDSVVKALAAPKPTAADLLTGV
YVSY

Sequences:

>Translated_324_residues
MTSQLKKEDLLRAYRTMREIREFEDRLHIEFATGKIPGFVHLYSGEEAVATGVCMHLTDEDRISSTHRGHGHCIAKGVDI
HGMMAEIYGKKTGTCGGKGGSMHIADLEKGMMGANGIVGAGPPLICGAALAAKFKGNGGVGVAFFGDGASNQGTILESMN
LASVWKLPVIFLNENNGYAESTGVSYNVPTPNIADRAAGFGMPGVTVDGNDFFAVYEAAGEAIRRARAGEGPTLLECKTQ
RYFGHFEGDAMTYRPNGESQNLRKTKDCLKIFAEKAIAAGLVTAAELEAIDKEVLALIEDSVVKALAAPKPTAADLLTGV
YVSY
>Mature_323_residues
TSQLKKEDLLRAYRTMREIREFEDRLHIEFATGKIPGFVHLYSGEEAVATGVCMHLTDEDRISSTHRGHGHCIAKGVDIH
GMMAEIYGKKTGTCGGKGGSMHIADLEKGMMGANGIVGAGPPLICGAALAAKFKGNGGVGVAFFGDGASNQGTILESMNL
ASVWKLPVIFLNENNGYAESTGVSYNVPTPNIADRAAGFGMPGVTVDGNDFFAVYEAAGEAIRRARAGEGPTLLECKTQR
YFGHFEGDAMTYRPNGESQNLRKTKDCLKIFAEKAIAAGLVTAAELEAIDKEVLALIEDSVVKALAAPKPTAADLLTGVY
VSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=324, Percent_Identity=37.037037037037, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI291084742, Length=324, Percent_Identity=37.962962962963, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4505685, Length=324, Percent_Identity=37.962962962963, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI291084744, Length=331, Percent_Identity=37.1601208459215, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI291084757, Length=324, Percent_Identity=33.641975308642, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=29.4670846394984, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI258645172, Length=319, Percent_Identity=29.4670846394984, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564172, Length=299, Percent_Identity=40.4682274247492, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17536047, Length=299, Percent_Identity=40.4682274247492, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI86563355, Length=314, Percent_Identity=28.9808917197452, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI86563357, Length=314, Percent_Identity=28.9808917197452, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=325, Percent_Identity=37.8461538461538, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24639744, Length=319, Percent_Identity=40.1253918495298, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28571106, Length=319, Percent_Identity=40.1253918495298, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24639740, Length=319, Percent_Identity=40.1253918495298, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=40.5228758169935, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24639748, Length=322, Percent_Identity=38.5093167701863, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21355903, Length=325, Percent_Identity=26.7692307692308, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34414; Mature: 34282

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQLKKEDLLRAYRTMREIREFEDRLHIEFATGKIPGFVHLYSGEEAVATGVCMHLTDE
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCHHHHCCEEEEECCC
DRISSTHRGHGHCIAKGVDIHGMMAEIYGKKTGTCGGKGGSMHIADLEKGMMGANGIVGA
HHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCC
GPPLICGAALAAKFKGNGGVGVAFFGDGASNQGTILESMNLASVWKLPVIFLNENNGYAE
CCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCEEECCCHHHEEECEEEEEECCCCCCC
STGVSYNVPTPNIADRAAGFGMPGVTVDGNDFFAVYEAAGEAIRRARAGEGPTLLECKTQ
CCCEEEECCCCCHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEHH
RYFGHFEGDAMTYRPNGESQNLRKTKDCLKIFAEKAIAAGLVTAAELEAIDKEVLALIED
HHCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVVKALAAPKPTAADLLTGVYVSY
HHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TSQLKKEDLLRAYRTMREIREFEDRLHIEFATGKIPGFVHLYSGEEAVATGVCMHLTDE
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCHHHHCCEEEEECCC
DRISSTHRGHGHCIAKGVDIHGMMAEIYGKKTGTCGGKGGSMHIADLEKGMMGANGIVGA
HHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCC
GPPLICGAALAAKFKGNGGVGVAFFGDGASNQGTILESMNLASVWKLPVIFLNENNGYAE
CCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCEEECCCHHHEEECEEEEEECCCCCCC
STGVSYNVPTPNIADRAAGFGMPGVTVDGNDFFAVYEAAGEAIRRARAGEGPTLLECKTQ
CCCEEEECCCCCHHHHHCCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEHH
RYFGHFEGDAMTYRPNGESQNLRKTKDCLKIFAEKAIAAGLVTAAELEAIDKEVLALIED
HHCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVVKALAAPKPTAADLLTGVYVSY
HHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]