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The map label for this gene is birA [H]

Identifier: 118579093

GI number: 118579093

Start: 692296

End: 693270

Strand: Direct

Name: birA [H]

Synonym: Ppro_0654

Alternate gene names: 118579093

Gene position: 692296-693270 (Clockwise)

Preceding gene: 118579092

Following gene: 118579094

Centisome position: 17.27

GC content: 63.28

Gene sequence:

>975_bases
ATGTTACCATCGGCCGCCATACTGCGACTGTTCCGTGAACAGGACGGATTCGTCTCGGGTGAGATGATCAGCAGGGAGTT
GCATGTCTCCCGCACCGCCATCTGGAAGCAGATCAATGCCCTGCGCAAGGCCGGGTACGTGATCGAAGCCGTGCCGTCGC
GCGGCTACCAGTTGCTGTCCGCGCCTGACCTGCTCTCCTCCGCCGAGATCGCGGTCCAATTGGATGCGGTCGTCATGGGC
AGCCGTATCGTCTGTCTGGAGCAGACCGCCTCCACCAACGGCGATGCTTTCCGCCTGGCGGAGCAGGGGGCCGCGGAGGG
GACGGTGGTCCTTGCCGATACCCAGAGCGGCGGTAAGGGGCGCATGGGGCGGGTCTGGTCATCGCCACCCGGGGTCAACC
TGTACTGCTCGATCATCCTGCGGCCGGCTATCATGCCCTACGAGGCGCCGCAACTCACCTTTCTCTCCGCCGTGGCTGTG
GCCCGTTCCATCGAACGGATCAGCGCTATCGTGCCGGAGATCAAGTGGCCCAATGACGTGCTGATCAAAGGACAGAAGGT
GGCCGGCCTGCTCAACGAAATGAGCGCCGAGACCGATGCTGTCAACTTCGTCATCCTCGGCATCGGTGTGAACCTGAACA
TGACTGCCGGGCAGTTCCCTGACGATCTGCGCCACCCGGCAACATCAATTTTGCTGGAGTCGGGGCGGCCGGTGGGGCGG
GCGGGCTTCTGCGCGATTATGCTGAACGAACTGGACCGGCTCTACGCCGATTTCCGCACCCATGGCTTCGGTCCGGTGCG
CGAGGAGTGGCAGCGGCGCTGCAACGCCCACGGACGCGAGCTTTCGGTCAGCGATGGCGGTTCGGAAGTTGTCAGAGGCA
TGTTCGCCGGCATTGATGGTTCCGGCGCCCTGCTGGTGAGCGGGGCGGATGGGGCGATCAGTAAAATTCTCAGTGGAGAC
GTGAGGGTACTTTGA

Upstream 100 bases:

>100_bases
CATTCGGCGCGGGCCATGGACATATCGATGCTGCTTGAATAGCCCGGACAGCCGTTTGATCCGGGCATGTTTTATCTTTC
TGGGAGGCTTGTAGCTGGAG

Downstream 100 bases:

>100_bases
CATGCTTCTGGTAATAGACGTCGGCAACAGCAATATCGTTCTGGGTATCTGCGACGGGACGCAGCTGGTCCGCAACTGGC
GGATCTCCACCGACAAATCG

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLPSAAILRLFREQDGFVSGEMISRELHVSRTAIWKQINALRKAGYVIEAVPSRGYQLLSAPDLLSSAEIAVQLDAVVMG
SRIVCLEQTASTNGDAFRLAEQGAAEGTVVLADTQSGGKGRMGRVWSSPPGVNLYCSIILRPAIMPYEAPQLTFLSAVAV
ARSIERISAIVPEIKWPNDVLIKGQKVAGLLNEMSAETDAVNFVILGIGVNLNMTAGQFPDDLRHPATSILLESGRPVGR
AGFCAIMLNELDRLYADFRTHGFGPVREEWQRRCNAHGRELSVSDGGSEVVRGMFAGIDGSGALLVSGADGAISKILSGD
VRVL

Sequences:

>Translated_324_residues
MLPSAAILRLFREQDGFVSGEMISRELHVSRTAIWKQINALRKAGYVIEAVPSRGYQLLSAPDLLSSAEIAVQLDAVVMG
SRIVCLEQTASTNGDAFRLAEQGAAEGTVVLADTQSGGKGRMGRVWSSPPGVNLYCSIILRPAIMPYEAPQLTFLSAVAV
ARSIERISAIVPEIKWPNDVLIKGQKVAGLLNEMSAETDAVNFVILGIGVNLNMTAGQFPDDLRHPATSILLESGRPVGR
AGFCAIMLNELDRLYADFRTHGFGPVREEWQRRCNAHGRELSVSDGGSEVVRGMFAGIDGSGALLVSGADGAISKILSGD
VRVL
>Mature_324_residues
MLPSAAILRLFREQDGFVSGEMISRELHVSRTAIWKQINALRKAGYVIEAVPSRGYQLLSAPDLLSSAEIAVQLDAVVMG
SRIVCLEQTASTNGDAFRLAEQGAAEGTVVLADTQSGGKGRMGRVWSSPPGVNLYCSIILRPAIMPYEAPQLTFLSAVAV
ARSIERISAIVPEIKWPNDVLIKGQKVAGLLNEMSAETDAVNFVILGIGVNLNMTAGQFPDDLRHPATSILLESGRPVGR
AGFCAIMLNELDRLYADFRTHGFGPVREEWQRRCNAHGRELSVSDGGSEVVRGMFAGIDGSGALLVSGADGAISKILSGD
VRVL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=306, Percent_Identity=31.3725490196078, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34660; Mature: 34660

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSAAILRLFREQDGFVSGEMISRELHVSRTAIWKQINALRKAGYVIEAVPSRGYQLLS
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEC
APDLLSSAEIAVQLDAVVMGSRIVCLEQTASTNGDAFRLAEQGAAEGTVVLADTQSGGKG
CCCHHCCCCEEEEEEEEEECCEEEEEECCCCCCCCEEEEHHCCCCCCEEEEEECCCCCCC
RMGRVWSSPPGVNLYCSIILRPAIMPYEAPQLTFLSAVAVARSIERISAIVPEIKWPNDV
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LIKGQKVAGLLNEMSAETDAVNFVILGIGVNLNMTAGQFPDDLRHPATSILLESGRPVGR
EEECHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCHHHHCCHHHHHHHCCCCCCC
AGFCAIMLNELDRLYADFRTHGFGPVREEWQRRCNAHGRELSVSDGGSEVVRGMFAGIDG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
SGALLVSGADGAISKILSGDVRVL
CCEEEEECCCHHHHHHHCCCCEEC
>Mature Secondary Structure
MLPSAAILRLFREQDGFVSGEMISRELHVSRTAIWKQINALRKAGYVIEAVPSRGYQLLS
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEC
APDLLSSAEIAVQLDAVVMGSRIVCLEQTASTNGDAFRLAEQGAAEGTVVLADTQSGGKG
CCCHHCCCCEEEEEEEEEECCEEEEEECCCCCCCCEEEEHHCCCCCCEEEEEECCCCCCC
RMGRVWSSPPGVNLYCSIILRPAIMPYEAPQLTFLSAVAVARSIERISAIVPEIKWPNDV
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
LIKGQKVAGLLNEMSAETDAVNFVILGIGVNLNMTAGQFPDDLRHPATSILLESGRPVGR
EEECHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCHHHHCCHHHHHHHCCCCCCC
AGFCAIMLNELDRLYADFRTHGFGPVREEWQRRCNAHGRELSVSDGGSEVVRGMFAGIDG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
SGALLVSGADGAISKILSGDVRVL
CCEEEEECCCHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]