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The map label for this gene is hycE [H]

Identifier: 118579033

GI number: 118579033

Start: 632434

End: 632913

Strand: Direct

Name: hycE [H]

Synonym: Ppro_0594

Alternate gene names: 118579033

Gene position: 632434-632913 (Clockwise)

Preceding gene: 118579032

Following gene: 118579034

Centisome position: 15.78

GC content: 56.25

Gene sequence:

>480_bases
ATGGCTGGTGAAGAAAAAGTACAACAGCAGTTTGTCAACCGCTTTCCCGAGCTGTCCGGCTCCATTCGGATAGTGCGCAG
CAGACGGATCTTCGTTGATGTGCCGGATCAGCGTTTTCTGGAGGTACTCGACTATGTTCGTGGAGAACTCGGTTTCCCCA
TTCTCTGTCTGATTGTCGGACTGGATGAGGGGGAAAATTTCGGGGTCATCTATATACTTGCGTCCGGTGACGGGACGCTG
GCCAGCCTCACGCGACGCATCCCCCGTGATGAGCCGGTGATCTGTTCCATCTACGATAGAATGCCCAATTCCGAGATCTA
TGAACGGGAACTGGTGGACATGTTCGGCATTCACGTGACCGGTCTGCCCGACGGTTCCCGCTATCCTCTGCCCGACGACT
GGCCCGAGGGACAATTCCCTTTGCGCAAGGACTGGACGCCCGACATGCTCGATCAGGTGGAGGTGGCCGTCAATGGGTAG

Upstream 100 bases:

>100_bases
TGTGGTGAAGCTGCTCACCACCCTGGAGAGGAAAAAGGCCGTCGAACCGCCGTCGATTAATCAGGATGTCTTACCACTTC
CCCAAGGAGGAAATTGATCA

Downstream 100 bases:

>100_bases
CGTTACGATTCCGATTGGTCCTCAGCATCCGGCACTGAAGGAGCCGGCCAATTTTTCCATAACCGTGGCTGGAGAGAAGA
TTCTGCACACAAGCTCCCGT

Product: NADH dehydrogenase (ubiquinone), 30 kDa subunit

Products: NAD+; ubiquinol

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAGEEKVQQQFVNRFPELSGSIRIVRSRRIFVDVPDQRFLEVLDYVRGELGFPILCLIVGLDEGENFGVIYILASGDGTL
ASLTRRIPRDEPVICSIYDRMPNSEIYERELVDMFGIHVTGLPDGSRYPLPDDWPEGQFPLRKDWTPDMLDQVEVAVNG

Sequences:

>Translated_159_residues
MAGEEKVQQQFVNRFPELSGSIRIVRSRRIFVDVPDQRFLEVLDYVRGELGFPILCLIVGLDEGENFGVIYILASGDGTL
ASLTRRIPRDEPVICSIYDRMPNSEIYERELVDMFGIHVTGLPDGSRYPLPDDWPEGQFPLRKDWTPDMLDQVEVAVNG
>Mature_158_residues
AGEEKVQQQFVNRFPELSGSIRIVRSRRIFVDVPDQRFLEVLDYVRGELGFPILCLIVGLDEGENFGVIYILASGDGTLA
SLTRRIPRDEPVICSIYDRMPNSEIYERELVDMFGIHVTGLPDGSRYPLPDDWPEGQFPLRKDWTPDMLDQVEVAVNG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789076, Length=151, Percent_Identity=32.4503311258278, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 18102; Mature: 17971

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEEKVQQQFVNRFPELSGSIRIVRSRRIFVDVPDQRFLEVLDYVRGELGFPILCLIVG
CCCHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEE
LDEGENFGVIYILASGDGTLASLTRRIPRDEPVICSIYDRMPNSEIYERELVDMFGIHVT
CCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEE
GLPDGSRYPLPDDWPEGQFPLRKDWTPDMLDQVEVAVNG
ECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECC
>Mature Secondary Structure 
AGEEKVQQQFVNRFPELSGSIRIVRSRRIFVDVPDQRFLEVLDYVRGELGFPILCLIVG
CCHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEE
LDEGENFGVIYILASGDGTLASLTRRIPRDEPVICSIYDRMPNSEIYERELVDMFGIHVT
CCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEE
GLPDGSRYPLPDDWPEGQFPLRKDWTPDMLDQVEVAVNG
ECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]