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The map label for this gene is hycG [C]

Identifier: 118579032

GI number: 118579032

Start: 631936

End: 632430

Strand: Direct

Name: hycG [C]

Synonym: Ppro_0593

Alternate gene names: 118579032

Gene position: 631936-632430 (Clockwise)

Preceding gene: 118579031

Following gene: 118579033

Centisome position: 15.77

GC content: 58.38

Gene sequence:

>495_bases
ATGAAAATCATCGACCGGATAGTCAACTGGGCGCGGATCAAATCCCCCTGGGTGATTCATTACAACACCGGCGCCTGCAA
CGGCTGCGACATCGAGATCGTGGCGACCCTGACGCCTGTGTTCGACATCGAGCGCTTTGGCTGCCAGCTGAAGGGGACTC
CGCGCCATGCCGATGTCCTGCTCTGCTCCGGCCCGGTAACCCGGCAGAGCAGGGATCGCATAATCCGCATTTATGAGCAG
ATGCCGGAACCGAAGTTCGTGGTTGCCGTCGGAAGCTGCGCCTGTTCCGGAGGAGTGTTCCAGGGCTGTTACAACATCCT
GGGAGGAATTGACAGCGTGATCCCTGTGTCCGCCTATGTTCCCGGATGTGCGGCACGGCCGGAAGCGATCATCGATGGTG
TGGTGAAGCTGCTCACCACCCTGGAGAGGAAAAAGGCCGTCGAACCGCCGTCGATTAATCAGGATGTCTTACCACTTCCC
CAAGGAGGAAATTGA

Upstream 100 bases:

>100_bases
ACCATACCACCCGGCTCATTGGCGTCATCCGCACTGGCGGTCCTGTTCACGCTCTGCGCGGCTCTGGGGCTCTTTGTACT
GTTCAGGAGGAACAACTGAC

Downstream 100 bases:

>100_bases
TCAATGGCTGGTGAAGAAAAAGTACAACAGCAGTTTGTCAACCGCTTTCCCGAGCTGTCCGGCTCCATTCGGATAGTGCG
CAGCAGACGGATCTTCGTTG

Product: NADH-quinone oxidoreductase subunit B

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKIIDRIVNWARIKSPWVIHYNTGACNGCDIEIVATLTPVFDIERFGCQLKGTPRHADVLLCSGPVTRQSRDRIIRIYEQ
MPEPKFVVAVGSCACSGGVFQGCYNILGGIDSVIPVSAYVPGCAARPEAIIDGVVKLLTTLERKKAVEPPSINQDVLPLP
QGGN

Sequences:

>Translated_164_residues
MKIIDRIVNWARIKSPWVIHYNTGACNGCDIEIVATLTPVFDIERFGCQLKGTPRHADVLLCSGPVTRQSRDRIIRIYEQ
MPEPKFVVAVGSCACSGGVFQGCYNILGGIDSVIPVSAYVPGCAARPEAIIDGVVKLLTTLERKKAVEPPSINQDVLPLP
QGGN
>Mature_164_residues
MKIIDRIVNWARIKSPWVIHYNTGACNGCDIEIVATLTPVFDIERFGCQLKGTPRHADVLLCSGPVTRQSRDRIIRIYEQ
MPEPKFVVAVGSCACSGGVFQGCYNILGGIDSVIPVSAYVPGCAARPEAIIDGVVKLLTTLERKKAVEPPSINQDVLPLP
QGGN

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=35.1724137931034, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1789074, Length=131, Percent_Identity=45.0381679389313, Blast_Score=131, Evalue=2e-32,
Organism=Escherichia coli, GI1788834, Length=131, Percent_Identity=43.5114503816794, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788624, Length=147, Percent_Identity=38.0952380952381, Blast_Score=110, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=37.9310344827586, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=35.8620689655172, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=35.8620689655172, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=35.1724137931034, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 17776; Mature: 17776

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIDRIVNWARIKSPWVIHYNTGACNGCDIEIVATLTPVFDIERFGCQLKGTPRHADVL
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCEEECCCCCCCCEE
LCSGPVTRQSRDRIIRIYEQMPEPKFVVAVGSCACSGGVFQGCYNILGGIDSVIPVSAYV
EECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHCC
PGCAARPEAIIDGVVKLLTTLERKKAVEPPSINQDVLPLPQGGN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIIDRIVNWARIKSPWVIHYNTGACNGCDIEIVATLTPVFDIERFGCQLKGTPRHADVL
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHCEEECCCCCCCCEE
LCSGPVTRQSRDRIIRIYEQMPEPKFVVAVGSCACSGGVFQGCYNILGGIDSVIPVSAYV
EECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHCC
PGCAARPEAIIDGVVKLLTTLERKKAVEPPSINQDVLPLPQGGN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]