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The map label for this gene is cooH [H]

Identifier: 118579034

GI number: 118579034

Start: 632906

End: 634135

Strand: Direct

Name: cooH [H]

Synonym: Ppro_0595

Alternate gene names: 118579034

Gene position: 632906-634135 (Clockwise)

Preceding gene: 118579033

Following gene: 118579035

Centisome position: 15.79

GC content: 59.27

Gene sequence:

>1230_bases
ATGGGTAGCGTTACGATTCCGATTGGTCCTCAGCATCCGGCACTGAAGGAGCCGGCCAATTTTTCCATAACCGTGGCTGG
AGAGAAGATTCTGCACACAAGCTCCCGTCTTGGCTACAACCACCGGGGCATGGAGAAGGCCTGTGAACAGCGGACCTATA
TCCAGGACCTGTATCTTCTGGAACGGATCTGCGGCATCTGCTCCCATTCGCACTCCATGTGCTTTGCCCAAGCCGTAGAG
GAAATAGCCGGCCTGAACGTTCCGCCCCGCGCCCTCTACATCCGCACCCTGATCGGAGAACTTGAACGGATCCACAGCCA
TCTGCTCTGGCTGGGGGTGGCGGGTCATGAGATCGGCTTCGACACCCTGCTGATGTACTCCTGGAGGGATCGGGAACTGG
TCATGGACCTTCTCGCGATGTTCAGCGGAAACCGGGTCAACTACGGCATCAACACCATCGGGGGCGTCAGGCGGGACGTC
AGCGACGAACAGATCGTCAAGGCATTGAACGACATCGATGCTCTGGAAGAACGGACCAAGTACTACATCGACATTGCCAC
AACCGAGCCGAGCTTCATCGCCAGGTTGGCGGGCGTGGGCTACATCTCCCATGAGGATGCGGTGCGTCTCCAGGCGGTGG
GGCCCACCGGACGCGGCTCCAACGTGCCCCACGACATGCGCGCCTGTGATCCCTACGCAGCCTATGGCGAGATGGACTTC
AAGGTGATCACGGACAGCAGCTGTGACGTGTACGGTCGGGCCGTGGTTAGGCTGCTGGAATTGATGGAGTCCTACAGGAT
CATACGTCAGGCGTTGCGGGAGATGCCGGATGGGCCGGTAAGCGTTAAGGCGCCGCGCACGATTCCGGCCGGTGATACGG
TCAGCCGTGTTGAGGCCCCGCGGGGTGAGGATCTGCACTACGTCAAGGGGAACGGTAGTGACAAGCCGGAGCGGGTCAAG
GTGCGCGCCTCCACCCTGGCTAATATCCAGGCCGTTAATAAGATGATGGAAAACAACAACCTGGCCGATGTGCCGATCAT
TATTGCGGCCATTGATCCCTGCTTCTCCTGCACCGACCGTTGTATCCAGCTCATCGGTAGCGAGCGCGGTGATATGGGGC
TGAAGGCGCTCAGGCAGCACGGTATCGACTGGTACCGGAAGAACCATGGTATCGAGTTCGGGGCTCTGGCGAAAAAACTG
CAAATGATGCGCGGGGAGCGGGCACTATGA

Upstream 100 bases:

>100_bases
GGTTCCCGCTATCCTCTGCCCGACGACTGGCCCGAGGGACAATTCCCTTTGCGCAAGGACTGGACGCCCGACATGCTCGA
TCAGGTGGAGGTGGCCGTCA

Downstream 100 bases:

>100_bases
CACAATCACTGTTCGGCATGTTCGTGTTTCCCGGTTTTCTCTTTCTCTGCGCCATCGGTGTTTGCGCCGAGTACTTCGAC
CGCATCTTCCATGCCCGCAT

Product: NADH dehydrogenase (ubiquinone)

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGSVTIPIGPQHPALKEPANFSITVAGEKILHTSSRLGYNHRGMEKACEQRTYIQDLYLLERICGICSHSHSMCFAQAVE
EIAGLNVPPRALYIRTLIGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDLLAMFSGNRVNYGINTIGGVRRDV
SDEQIVKALNDIDALEERTKYYIDIATTEPSFIARLAGVGYISHEDAVRLQAVGPTGRGSNVPHDMRACDPYAAYGEMDF
KVITDSSCDVYGRAVVRLLELMESYRIIRQALREMPDGPVSVKAPRTIPAGDTVSRVEAPRGEDLHYVKGNGSDKPERVK
VRASTLANIQAVNKMMENNNLADVPIIIAAIDPCFSCTDRCIQLIGSERGDMGLKALRQHGIDWYRKNHGIEFGALAKKL
QMMRGERAL

Sequences:

>Translated_409_residues
MGSVTIPIGPQHPALKEPANFSITVAGEKILHTSSRLGYNHRGMEKACEQRTYIQDLYLLERICGICSHSHSMCFAQAVE
EIAGLNVPPRALYIRTLIGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDLLAMFSGNRVNYGINTIGGVRRDV
SDEQIVKALNDIDALEERTKYYIDIATTEPSFIARLAGVGYISHEDAVRLQAVGPTGRGSNVPHDMRACDPYAAYGEMDF
KVITDSSCDVYGRAVVRLLELMESYRIIRQALREMPDGPVSVKAPRTIPAGDTVSRVEAPRGEDLHYVKGNGSDKPERVK
VRASTLANIQAVNKMMENNNLADVPIIIAAIDPCFSCTDRCIQLIGSERGDMGLKALRQHGIDWYRKNHGIEFGALAKKL
QMMRGERAL
>Mature_408_residues
GSVTIPIGPQHPALKEPANFSITVAGEKILHTSSRLGYNHRGMEKACEQRTYIQDLYLLERICGICSHSHSMCFAQAVEE
IAGLNVPPRALYIRTLIGELERIHSHLLWLGVAGHEIGFDTLLMYSWRDRELVMDLLAMFSGNRVNYGINTIGGVRRDVS
DEQIVKALNDIDALEERTKYYIDIATTEPSFIARLAGVGYISHEDAVRLQAVGPTGRGSNVPHDMRACDPYAAYGEMDFK
VITDSSCDVYGRAVVRLLELMESYRIIRQALREMPDGPVSVKAPRTIPAGDTVSRVEAPRGEDLHYVKGNGSDKPERVKV
RASTLANIQAVNKMMENNNLADVPIIIAAIDPCFSCTDRCIQLIGSERGDMGLKALRQHGIDWYRKNHGIEFGALAKKLQ
MMRGERAL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=392, Percent_Identity=27.8061224489796, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI260898743, Length=382, Percent_Identity=27.4869109947644, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1789076, Length=356, Percent_Identity=37.6404494382022, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788832, Length=381, Percent_Identity=35.6955380577428, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI145693162, Length=383, Percent_Identity=26.3707571801567, Blast_Score=161, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17555284, Length=394, Percent_Identity=29.1878172588832, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17568379, Length=385, Percent_Identity=28.3116883116883, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24638644, Length=385, Percent_Identity=30.1298701298701, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI221459469, Length=382, Percent_Identity=30.6282722513089, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 45495; Mature: 45364

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVTIPIGPQHPALKEPANFSITVAGEKILHTSSRLGYNHRGMEKACEQRTYIQDLYLL
CCCEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERICGICSHSHSMCFAQAVEEIAGLNVPPRALYIRTLIGELERIHSHLLWLGVAGHEIGF
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
DTLLMYSWRDRELVMDLLAMFSGNRVNYGINTIGGVRRDVSDEQIVKALNDIDALEERTK
HHEEEECCCCHHHHHHHHHHHCCCCEEECCHHHCHHHCCCCHHHHHHHHHHHHHHHHCCE
YYIDIATTEPSFIARLAGVGYISHEDAVRLQAVGPTGRGSNVPHDMRACDPYAAYGEMDF
EEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCHHCCCCEE
KVITDSSCDVYGRAVVRLLELMESYRIIRQALREMPDGPVSVKAPRTIPAGDTVSRVEAP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHCCC
RGEDLHYVKGNGSDKPERVKVRASTLANIQAVNKMMENNNLADVPIIIAAIDPCFSCTDR
CCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
CIQLIGSERGDMGLKALRQHGIDWYRKNHGIEFGALAKKLQMMRGERAL
HHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GSVTIPIGPQHPALKEPANFSITVAGEKILHTSSRLGYNHRGMEKACEQRTYIQDLYLL
CCEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ERICGICSHSHSMCFAQAVEEIAGLNVPPRALYIRTLIGELERIHSHLLWLGVAGHEIGF
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
DTLLMYSWRDRELVMDLLAMFSGNRVNYGINTIGGVRRDVSDEQIVKALNDIDALEERTK
HHEEEECCCCHHHHHHHHHHHCCCCEEECCHHHCHHHCCCCHHHHHHHHHHHHHHHHCCE
YYIDIATTEPSFIARLAGVGYISHEDAVRLQAVGPTGRGSNVPHDMRACDPYAAYGEMDF
EEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCHHCCCCEE
KVITDSSCDVYGRAVVRLLELMESYRIIRQALREMPDGPVSVKAPRTIPAGDTVSRVEAP
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHCCC
RGEDLHYVKGNGSDKPERVKVRASTLANIQAVNKMMENNNLADVPIIIAAIDPCFSCTDR
CCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
CIQLIGSERGDMGLKALRQHGIDWYRKNHGIEFGALAKKLQMMRGERAL
HHHHHCCCCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]