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The map label for this gene is trmFO

Identifier: 118578966

GI number: 118578966

Start: 560756

End: 562120

Strand: Reverse

Name: trmFO

Synonym: Ppro_0527

Alternate gene names: 118578966

Gene position: 562120-560756 (Counterclockwise)

Preceding gene: 118578967

Following gene: 118578964

Centisome position: 14.02

GC content: 67.25

Gene sequence:

>1365_bases
ATGAAACACTCCCTCACCATCATCGGCGCCGGCCTGGCAGGCTGCGAGGCCGCTTGGCAGGCCGCGCGGCGCGGCGTCAG
CGTCACCCTGCACGAAATGAAGCCCGAAAAGCGCTCTCCCGCCCACCATCTCCCCGGGCTGGCCGAACTGGTCTGCTCCA
ACTCCCTGCGGGGCGATTCCCTGGAAAACGCCGTAGGGCTCTTGAAGGAAGAGCTCAGGCGCGGCGGCTCCCTAATCATG
GAGGCCGCCGAGGCCACCCGCGTCCCGGCCGGCGGAGCCCTGGCGGTGGACCGCCAGCTCTTCTCCGACTATGTCACGGC
AAAGATCACGGCGCATCCCCTGATCAACCTGGTGCATGGCGAAATGGATGCCATCCCCGCCGAGGGGACGGTGATCATCG
CCTCCGGCCCCCTGACCAGCGACGCCCTGGCCGCCTCCCTCTCCGGCCTGACCGGCGACCGGCTCTACTTCTACGATTCC
ATCGCCCCCATCGTCACGGCGGATTCCCTGGACCGGGAGAAAGTCTTCGCGGCGTCGCGCTACGGCAAGGGGGACGGCGA
CGACTACCTCAACTGCCCCCTGGACCGGGAGCAGTACCAGGCCTTTGTGGAGCAGCTAAACAGGGGGGAGAAGGTAGCGG
CACGCGAGTTCGAGAAGCTCGTGCACTTCGAGGGATGCATGCCGGTGGAGGAACTGGCCGGGCGGGGGGAGGACACGCTC
CGCTTCGGCCCCATGAAACCGGTGGGCCTGACGGATCCCCGCACGGGCATCGAACCGCACGCGGTGATCCAGCTGCGCGC
CGAGAACCGGGAGAAAACCATGTACAACCTGGTCGGCTTCCAGACCAAGCTGACCTGGCCGGAACAGCGCCGCATATTCA
GGACCATACCGGGGCTGGAGCGAGCCGAGTTCGTGCGGCTCGGGTCCATGCACCGCAACACCTTCATCAACGCCCCTGCC
CTGCTCCAGCCGACCCAGCAGCTGAGGAGCGATCCGCGCATATTCTTCGCAGGCCAGATCACCGGCGTTGAGGGGTACGT
GGAATCGGCGGGGAGCGGCTTTTTGGCCGGCATCACCGCCGCCCGCCTGCTCACGGGGATGCCGGACCCGGCCGTGCCCC
CTGCCGAGACCGCACTGGGCGCCTTGGTCAGCCACATCACCAATGCCGATGTGCGCCACTTCCAGCCCATGAACGTGAAC
TACGGCCTGTTCCCCGATCTGGAGGGACGGATCAAGAAAAAGGAGCGGCGCGGCAAACTGGCCGAGCGGGCCCTGGCCTC
CCTGTCGGGCTGGTTGGAACGGGTATCCCCCCCGAGCCGGGAGACGGGAGAGCCGACCGGGGCGGAGCAGGTTGATCTCG
CCTGA

Upstream 100 bases:

>100_bases
GATCGTCAACCATTACACCTGATCACCACGAGCAGCGCCCGTCACCCTGGAAAGCGAGTGGCTAGGGACGTTTGCACAGA
ACTCAGACGGGATACATACC

Downstream 100 bases:

>100_bases
TTCCCCGTTTACGGCATTGCCACAAACGGATGATTTCTTTACACTAGGAGTCTGTCGGGCTTGACCTCAGGGGCGCCCTC
TTTTGTGGCCAAGCCGATTA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKHSLTIIGAGLAGCEAAWQAARRGVSVTLHEMKPEKRSPAHHLPGLAELVCSNSLRGDSLENAVGLLKEELRRGGSLIM
EAAEATRVPAGGALAVDRQLFSDYVTAKITAHPLINLVHGEMDAIPAEGTVIIASGPLTSDALAASLSGLTGDRLYFYDS
IAPIVTADSLDREKVFAASRYGKGDGDDYLNCPLDREQYQAFVEQLNRGEKVAAREFEKLVHFEGCMPVEELAGRGEDTL
RFGPMKPVGLTDPRTGIEPHAVIQLRAENREKTMYNLVGFQTKLTWPEQRRIFRTIPGLERAEFVRLGSMHRNTFINAPA
LLQPTQQLRSDPRIFFAGQITGVEGYVESAGSGFLAGITAARLLTGMPDPAVPPAETALGALVSHITNADVRHFQPMNVN
YGLFPDLEGRIKKKERRGKLAERALASLSGWLERVSPPSRETGEPTGAEQVDLA

Sequences:

>Translated_454_residues
MKHSLTIIGAGLAGCEAAWQAARRGVSVTLHEMKPEKRSPAHHLPGLAELVCSNSLRGDSLENAVGLLKEELRRGGSLIM
EAAEATRVPAGGALAVDRQLFSDYVTAKITAHPLINLVHGEMDAIPAEGTVIIASGPLTSDALAASLSGLTGDRLYFYDS
IAPIVTADSLDREKVFAASRYGKGDGDDYLNCPLDREQYQAFVEQLNRGEKVAAREFEKLVHFEGCMPVEELAGRGEDTL
RFGPMKPVGLTDPRTGIEPHAVIQLRAENREKTMYNLVGFQTKLTWPEQRRIFRTIPGLERAEFVRLGSMHRNTFINAPA
LLQPTQQLRSDPRIFFAGQITGVEGYVESAGSGFLAGITAARLLTGMPDPAVPPAETALGALVSHITNADVRHFQPMNVN
YGLFPDLEGRIKKKERRGKLAERALASLSGWLERVSPPSRETGEPTGAEQVDLA
>Mature_454_residues
MKHSLTIIGAGLAGCEAAWQAARRGVSVTLHEMKPEKRSPAHHLPGLAELVCSNSLRGDSLENAVGLLKEELRRGGSLIM
EAAEATRVPAGGALAVDRQLFSDYVTAKITAHPLINLVHGEMDAIPAEGTVIIASGPLTSDALAASLSGLTGDRLYFYDS
IAPIVTADSLDREKVFAASRYGKGDGDDYLNCPLDREQYQAFVEQLNRGEKVAAREFEKLVHFEGCMPVEELAGRGEDTL
RFGPMKPVGLTDPRTGIEPHAVIQLRAENREKTMYNLVGFQTKLTWPEQRRIFRTIPGLERAEFVRLGSMHRNTFINAPA
LLQPTQQLRSDPRIFFAGQITGVEGYVESAGSGFLAGITAARLLTGMPDPAVPPAETALGALVSHITNADVRHFQPMNVN
YGLFPDLEGRIKKKERRGKLAERALASLSGWLERVSPPSRETGEPTGAEQVDLA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=108, Percent_Identity=37.962962962963, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PELPD (A1ALD8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900216.1
- ProteinModelPortal:   A1ALD8
- STRING:   A1ALD8
- GeneID:   4572709
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0527
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49293; Mature: 49293

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSLTIIGAGLAGCEAAWQAARRGVSVTLHEMKPEKRSPAHHLPGLAELVCSNSLRGDS
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCHHHHHHHCCCCCCCH
LENAVGLLKEELRRGGSLIMEAAEATRVPAGGALAVDRQLFSDYVTAKITAHPLINLVHG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHC
EMDAIPAEGTVIIASGPLTSDALAASLSGLTGDRLYFYDSIAPIVTADSLDREKVFAASR
HHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHC
YGKGDGDDYLNCPLDREQYQAFVEQLNRGEKVAAREFEKLVHFEGCMPVEELAGRGEDTL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCE
RFGPMKPVGLTDPRTGIEPHAVIQLRAENREKTMYNLVGFQTKLTWPEQRRIFRTIPGLE
EECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCEEECCCHHHHHHHHHCCCCC
RAEFVRLGSMHRNTFINAPALLQPTQQLRSDPRIFFAGQITGVEGYVESAGSGFLAGITA
HHHHHHHCCHHHHHCCCCCHHCCCHHHHCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHH
ARLLTGMPDPAVPPAETALGALVSHITNADVRHFQPMNVNYGLFPDLEGRIKKKERRGKL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AERALASLSGWLERVSPPSRETGEPTGAEQVDLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHSLTIIGAGLAGCEAAWQAARRGVSVTLHEMKPEKRSPAHHLPGLAELVCSNSLRGDS
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCHHHHHHHCCCCCCCH
LENAVGLLKEELRRGGSLIMEAAEATRVPAGGALAVDRQLFSDYVTAKITAHPLINLVHG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHC
EMDAIPAEGTVIIASGPLTSDALAASLSGLTGDRLYFYDSIAPIVTADSLDREKVFAASR
HHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHC
YGKGDGDDYLNCPLDREQYQAFVEQLNRGEKVAAREFEKLVHFEGCMPVEELAGRGEDTL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCE
RFGPMKPVGLTDPRTGIEPHAVIQLRAENREKTMYNLVGFQTKLTWPEQRRIFRTIPGLE
EECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCEEECCCHHHHHHHHHCCCCC
RAEFVRLGSMHRNTFINAPALLQPTQQLRSDPRIFFAGQITGVEGYVESAGSGFLAGITA
HHHHHHHCCHHHHHCCCCCHHCCCHHHHCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHH
ARLLTGMPDPAVPPAETALGALVSHITNADVRHFQPMNVNYGLFPDLEGRIKKKERRGKL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
AERALASLSGWLERVSPPSRETGEPTGAEQVDLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA