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The map label for this gene is glcF [H]

Identifier: 118578964

GI number: 118578964

Start: 557699

End: 558985

Strand: Reverse

Name: glcF [H]

Synonym: Ppro_0525

Alternate gene names: 118578964

Gene position: 558985-557699 (Counterclockwise)

Preceding gene: 118578966

Following gene: 118578963

Centisome position: 13.95

GC content: 65.27

Gene sequence:

>1287_bases
ATGATGGAACAGCAGACCAACGCACCTCTGACTAGGATCCAGGACCAGCTGAAAAAATGCGTCAAATGCGGCGCCTGCCG
CGCCAACTGCCCGGCCTTCAGCGTCTTTGGTCGCGAACCGGCCGTGGCCCGCGGCAAGGTGGCTCTGGCCCAGCACATCC
TCAAGGACGACATCGGCCTGGACGACCAGACCTATCTGGCCATGTCCAAGTGCCTGCTGTGCGGCAGCTGCGTTGAAAAG
TGTCCCAACGACGTACCCACCGACGAGATCGTCATCGCCGCCCGCGAGGCCCTGGCCCGCAAACGCGGGCTGACCACCTT
CCACGCGGCGGTGGGCCGAGTGATCAAAAACCGCTCCCTGATGACCATGGGGGCACGGATGGCCGGGCTGCTGGGCCCGC
TCTTCTTCAAGAAAGTCCCGGCCACCTCCGGGCTGCGCCTGCGCTTTCCGGCCCCCTTCGTCGGCAACCAGCGCCACATA
CCGGCCATTGCCCGGACCCCCTTTATGGACCGCCACCCGGAGGTGATACCGGGTGAGCCGGGCAAACCGCGGGTGATCTT
CTTCGTGGGATGCATGACCAACTTCGTCTACACTTCCGTGGGCGAAGCGGCCCTGGCGCTCCTGCGCCACCTGGGCTGCA
CCGTGATCATCCCCAAGGGACAGCAGTGCTGCGGCCTGCCCGCCATGTCGGGCGGCGACATCGCCACGGTGCGCGAGCTG
GCCGAGAAGAACCTGACGGAAATGGAGCGCTACCAGGCGGATTACGTGATGAGCGCCTGCGCCACCTGCGGAGGCGCCCT
GCATCGCCTCTACCCGCTGACCGTCGGCAAGCGCAACCCCGAACTGAAGGCGCGCCTGGAGGCCCTGGCCAAAAAAACGG
TGGATGCCTCGCAACTGCTGCACCAGCTGGGACTCAACCCGGCCGAAACCGGGGCCGGCGATGCCCTGCGCATTACCTAC
CATGACCCCTGCCACCTGCGCACCCGCGGCTTAACCGGACAGCCCCGAAACCTGCTGCGCGCCGCCCCCGGCCTGGAGCT
TACGGAAATGGAATTCGCCGACCGCTGCTGCGGCCTCGGCGGCACCTTCAACGTCTACCACTACGACTCATCCATGAGCA
TCAACGAAGCCAAGAGCCGTGCCATACTCAAAACCGCCGCCCAGGCAGTTGCCACCGGCTGCCCCGGCTGCATGATGCAG
CTTTCCGACGGCCTCAAGCGACAGGGCTCAAAGGTCGAGGTCGTGCACACCCTTGAGCTGCTTGCACGCAAACTGGCAAA
GCCATAA

Upstream 100 bases:

>100_bases
CCAATTGAAAACGCCCTGGAGATGACGGCTGAATGCGTTAAGCCCGACAGACTCCTAGCACTACCGTTTTCTCCCCACCG
CTCCCGCGGGAAAGGTTTTC

Downstream 100 bases:

>100_bases
ACATCGTTTTGCTTTTCATTCCACTAAAATCCAATTATTTTTCCCGCCGCCATTTAACCGGAATTTAACGCAAAATCCGA
TCAATAAACTTTTGTTACCA

Product: hypothetical protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MMEQQTNAPLTRIQDQLKKCVKCGACRANCPAFSVFGREPAVARGKVALAQHILKDDIGLDDQTYLAMSKCLLCGSCVEK
CPNDVPTDEIVIAAREALARKRGLTTFHAAVGRVIKNRSLMTMGARMAGLLGPLFFKKVPATSGLRLRFPAPFVGNQRHI
PAIARTPFMDRHPEVIPGEPGKPRVIFFVGCMTNFVYTSVGEAALALLRHLGCTVIIPKGQQCCGLPAMSGGDIATVREL
AEKNLTEMERYQADYVMSACATCGGALHRLYPLTVGKRNPELKARLEALAKKTVDASQLLHQLGLNPAETGAGDALRITY
HDPCHLRTRGLTGQPRNLLRAAPGLELTEMEFADRCCGLGGTFNVYHYDSSMSINEAKSRAILKTAAQAVATGCPGCMMQ
LSDGLKRQGSKVEVVHTLELLARKLAKP

Sequences:

>Translated_428_residues
MMEQQTNAPLTRIQDQLKKCVKCGACRANCPAFSVFGREPAVARGKVALAQHILKDDIGLDDQTYLAMSKCLLCGSCVEK
CPNDVPTDEIVIAAREALARKRGLTTFHAAVGRVIKNRSLMTMGARMAGLLGPLFFKKVPATSGLRLRFPAPFVGNQRHI
PAIARTPFMDRHPEVIPGEPGKPRVIFFVGCMTNFVYTSVGEAALALLRHLGCTVIIPKGQQCCGLPAMSGGDIATVREL
AEKNLTEMERYQADYVMSACATCGGALHRLYPLTVGKRNPELKARLEALAKKTVDASQLLHQLGLNPAETGAGDALRITY
HDPCHLRTRGLTGQPRNLLRAAPGLELTEMEFADRCCGLGGTFNVYHYDSSMSINEAKSRAILKTAAQAVATGCPGCMMQ
LSDGLKRQGSKVEVVHTLELLARKLAKP
>Mature_428_residues
MMEQQTNAPLTRIQDQLKKCVKCGACRANCPAFSVFGREPAVARGKVALAQHILKDDIGLDDQTYLAMSKCLLCGSCVEK
CPNDVPTDEIVIAAREALARKRGLTTFHAAVGRVIKNRSLMTMGARMAGLLGPLFFKKVPATSGLRLRFPAPFVGNQRHI
PAIARTPFMDRHPEVIPGEPGKPRVIFFVGCMTNFVYTSVGEAALALLRHLGCTVIIPKGQQCCGLPAMSGGDIATVREL
AEKNLTEMERYQADYVMSACATCGGALHRLYPLTVGKRNPELKARLEALAKKTVDASQLLHQLGLNPAETGAGDALRITY
HDPCHLRTRGLTGQPRNLLRAAPGLELTEMEFADRCCGLGGTFNVYHYDSSMSINEAKSRAILKTAAQAVATGCPGCMMQ
LSDGLKRQGSKVEVVHTLELLARKLAKP

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=417, Percent_Identity=25.8992805755396, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786497, Length=242, Percent_Identity=30.9917355371901, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994913, Length=440, Percent_Identity=25.4545454545455, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46340; Mature: 46340

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEQQTNAPLTRIQDQLKKCVKCGACRANCPAFSVFGREPAVARGKVALAQHILKDDIGL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
DDQTYLAMSKCLLCGSCVEKCPNDVPTDEIVIAAREALARKRGLTTFHAAVGRVIKNRSL
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
MTMGARMAGLLGPLFFKKVPATSGLRLRFPAPFVGNQRHIPAIARTPFMDRHPEVIPGEP
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
GKPRVIFFVGCMTNFVYTSVGEAALALLRHLGCTVIIPKGQQCCGLPAMSGGDIATVREL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
AEKNLTEMERYQADYVMSACATCGGALHRLYPLTVGKRNPELKARLEALAKKTVDASQLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
HQLGLNPAETGAGDALRITYHDPCHLRTRGLTGQPRNLLRAAPGLELTEMEFADRCCGLG
HHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCC
GTFNVYHYDSSMSINEAKSRAILKTAAQAVATGCPGCMMQLSDGLKRQGSKVEVVHTLEL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHHHHHHHH
LARKLAKP
HHHHHCCC
>Mature Secondary Structure
MMEQQTNAPLTRIQDQLKKCVKCGACRANCPAFSVFGREPAVARGKVALAQHILKDDIGL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
DDQTYLAMSKCLLCGSCVEKCPNDVPTDEIVIAAREALARKRGLTTFHAAVGRVIKNRSL
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
MTMGARMAGLLGPLFFKKVPATSGLRLRFPAPFVGNQRHIPAIARTPFMDRHPEVIPGEP
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
GKPRVIFFVGCMTNFVYTSVGEAALALLRHLGCTVIIPKGQQCCGLPAMSGGDIATVREL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
AEKNLTEMERYQADYVMSACATCGGALHRLYPLTVGKRNPELKARLEALAKKTVDASQLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
HQLGLNPAETGAGDALRITYHDPCHLRTRGLTGQPRNLLRAAPGLELTEMEFADRCCGLG
HHHCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCC
GTFNVYHYDSSMSINEAKSRAILKTAAQAVATGCPGCMMQLSDGLKRQGSKVEVVHTLEL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEHHHHHHHH
LARKLAKP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]