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The map label for this gene is topA [H]

Identifier: 118578967

GI number: 118578967

Start: 562324

End: 564654

Strand: Reverse

Name: topA [H]

Synonym: Ppro_0528

Alternate gene names: 118578967

Gene position: 564654-562324 (Counterclockwise)

Preceding gene: 118578968

Following gene: 118578966

Centisome position: 14.09

GC content: 63.06

Gene sequence:

>2331_bases
ATGCCGCAGAACCTCGTCATCGTTGAGTCGCCCGCCAAGGCGAAGACCATAGAGAAATTCCTGGGAAAGGACTACAAGGT
ACTGGCCTCCTTCGGCCACGTGAGGGCCCTGCCCAGCAAGCAGGGGTCGGTGGATATCGAACAGGACTTCACCCCCAAGT
ACCACATCCTCCCCGAGAGCAAGAAACATATCGACGCCATCAAGGCAGCCCTCAAGGACGCCAAGAACCTGCTCCTGGCA
ACCGACCCCGACCGCGAGGGAGAGGCCATCTCCTGGCACCTGCTGGCGGCCCTGGGGCTGGACAAGAAGAATCCGGGCAA
GTCCATCCAGCGGGTGGTCTTCCACGAGATCACCAAAGACGCCATCATCCACGCCGTGGAGAATCCCCGTGACATCGCCA
CGGACCTGGTGGATGCCCAGCAGGCGCGCTCGGTGCTGGACTACCTGGTCGGCTTCAACCTTTCCCCCTTCCTCTGGAAG
AAGATCCGCTACGGCCTCTCGGCCGGGCGCGTCCAGTCGGTGGCGCTGCGCCTGGTCTGCGAGCGGGAGAAAGAGATTCA
GGCCTTCAAGGAACAGGAGTACTGGACCATCGCCGCGCGCCTGGGGGTAAAGCCGGGAGCGGAGTTCCGGGCCTCGCTGG
TGGAGGTTGGCGGCAAAAAACTGGGCAAGTTCGACATCCCCGACGAGAACGTGGCAACGGCACTGAACCGGGCACTGAAG
TCGGGGAGCTACCGGGTGGCAAAGATCACCAGGACGGAACGCAAGCGCAACCCGTCGCCCCCCTTCACCACCTCGACCCT
GCAGCAGGAAGCTTCCCGCAAGCTGGGGTTCTCGGCCAAGAAGACCATGTCAACGGCCCAGAAACTGTACGAGGGGATCG
ACATCGGCCAGGAGGGGACCGTCGGCCTGATCACCTACATGCGTACCGACAGCGTCACCCTCTCCAGCCAGGCTTTGACC
GAGGCCCACGAGGTCATCAGCGCGGCCTACGGAGCAGAGTATGCCCTGGCCAAACCGCGCATCTTCAAGACCAAGACCAA
GAATGCCCAGGAGGCCCACGAGGCGGTGCGCCCCACCTCCATCGCCCGCACACCGGCGGAGCTGAAGAAGCACCTCACCC
CCGACCTGTTCAAGCTCTACGAACTGATCTGGAAGCGCACCGTGGCCAGCCAGATGGCCGAGGCGCTCCTGGACCAGACC
TCGGTTGACATAGCGGCCGAGTTGAATGAGGCGGTGGCGGACTGCACAGTCGCCGAGTCCGGCGCAGCCAGCCCCTTTAC
GGGCGCAAAGCGCTGCGGCCTGCGTGCGGCCGGCACGGTGATCCGCTTCCCCGGCTTCATGAAGCTGTACATCGAGGGAC
TGGACGACCAGGACGAGGAGAAAGAGGGGCTGCTCCCCCCCTTGCAGGAGGGGGACGACCTGAAATTGCACGAACTGTTT
CCCGAACAGCACTTCACCCAGCCGCCGCCGCGCTACACCGAGGCCACCCTGGTCAAGACCCTGGAGGAGTACGGCATCGG
CCGCCCCTCCACCTACGCCTCGATCATGAACACCCTGGTTGAGCGCAAGTACACCCGCCTGGACAAGAAGCGCTTCTTCC
CCGAGGATGTGGGCATGGTGGTCAACGACCTGCTGGTGGCCCACTTCTCCCGCTACGTGGATTACAACTTCACCGCCGGC
CTGGAGGAAGAGCTGGACCAGGTTTCCCGGGGGGAGAAGCAGTGGAAGCCGCTGCTGAAAGAGTTCTGGGAGCCGTTCAT
CACCCTGCTGAAGCAGAAGGAGGGGGAGGTCAAGAAGGACGACTTGACCACCACGGAACTGGACGAGGTCTGCCCGGAAT
GCGGCAAACCGTTGGCCATCAAACTGGGCAAGCGGGGCAAGTTCATCGCCTGCACCGGCTTCAAGGAGGGGTGCACCTAC
ACCCGCAACCTGGAATCCGACGCCAGCGAGCCGTCCGAGCCGGTGCTCTCCGATGAGAAGTGCGACAAGTGCGGTCAGCA
GATGCTGATCAAGGACGGCCGCTATGGAAAGTACCTGGCCTGCTCCGGCTATCCGGCCTGCAAGAACATCCAGCCGCTGG
TCAAGCCGCGCGGCACCGGCGTGGTCTGCCCCGAGTGCAAGGAAGGCGAGCTGACCGAGAAGAAATCACGCTTCGGCAAA
CTGTTCTACTCCTGCAACCGCTACCCCCAGTGCAAGTTCGCCCTCTGGGACCCGCCGGTGGAGCAGCCCTGTCCCAAATG
CGGCTTCCCGCTGCTGGTGAAGAAGGTCTACAAGCGCAAGGGTGAATTCCTCAAATGCCCCAAGGAAGGGTGCGACTACA
CCAGCGAGTGA

Upstream 100 bases:

>100_bases
CCGCCGCCGAGCCTGTATTCACGGCGCAGGATAGGGGGTGCGCCACGGGCAATGATCCGCTGCGCGCCAAAATCCACCAC
CAAGGGACAGCAGAGACACC

Downstream 100 bases:

>100_bases
GGCAACAACCGCTGGAGCGAATTGCAGCCCCACGAGCGGATACGCACCACTGTTCGACCCCGCGGAAAGATACGCGGGGT
CATTTTTTTAGCCTTTTGAC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MPQNLVIVESPAKAKTIEKFLGKDYKVLASFGHVRALPSKQGSVDIEQDFTPKYHILPESKKHIDAIKAALKDAKNLLLA
TDPDREGEAISWHLLAALGLDKKNPGKSIQRVVFHEITKDAIIHAVENPRDIATDLVDAQQARSVLDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLVCEREKEIQAFKEQEYWTIAARLGVKPGAEFRASLVEVGGKKLGKFDIPDENVATALNRALK
SGSYRVAKITRTERKRNPSPPFTTSTLQQEASRKLGFSAKKTMSTAQKLYEGIDIGQEGTVGLITYMRTDSVTLSSQALT
EAHEVISAAYGAEYALAKPRIFKTKTKNAQEAHEAVRPTSIARTPAELKKHLTPDLFKLYELIWKRTVASQMAEALLDQT
SVDIAAELNEAVADCTVAESGAASPFTGAKRCGLRAAGTVIRFPGFMKLYIEGLDDQDEEKEGLLPPLQEGDDLKLHELF
PEQHFTQPPPRYTEATLVKTLEEYGIGRPSTYASIMNTLVERKYTRLDKKRFFPEDVGMVVNDLLVAHFSRYVDYNFTAG
LEEELDQVSRGEKQWKPLLKEFWEPFITLLKQKEGEVKKDDLTTTELDEVCPECGKPLAIKLGKRGKFIACTGFKEGCTY
TRNLESDASEPSEPVLSDEKCDKCGQQMLIKDGRYGKYLACSGYPACKNIQPLVKPRGTGVVCPECKEGELTEKKSRFGK
LFYSCNRYPQCKFALWDPPVEQPCPKCGFPLLVKKVYKRKGEFLKCPKEGCDYTSE

Sequences:

>Translated_776_residues
MPQNLVIVESPAKAKTIEKFLGKDYKVLASFGHVRALPSKQGSVDIEQDFTPKYHILPESKKHIDAIKAALKDAKNLLLA
TDPDREGEAISWHLLAALGLDKKNPGKSIQRVVFHEITKDAIIHAVENPRDIATDLVDAQQARSVLDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLVCEREKEIQAFKEQEYWTIAARLGVKPGAEFRASLVEVGGKKLGKFDIPDENVATALNRALK
SGSYRVAKITRTERKRNPSPPFTTSTLQQEASRKLGFSAKKTMSTAQKLYEGIDIGQEGTVGLITYMRTDSVTLSSQALT
EAHEVISAAYGAEYALAKPRIFKTKTKNAQEAHEAVRPTSIARTPAELKKHLTPDLFKLYELIWKRTVASQMAEALLDQT
SVDIAAELNEAVADCTVAESGAASPFTGAKRCGLRAAGTVIRFPGFMKLYIEGLDDQDEEKEGLLPPLQEGDDLKLHELF
PEQHFTQPPPRYTEATLVKTLEEYGIGRPSTYASIMNTLVERKYTRLDKKRFFPEDVGMVVNDLLVAHFSRYVDYNFTAG
LEEELDQVSRGEKQWKPLLKEFWEPFITLLKQKEGEVKKDDLTTTELDEVCPECGKPLAIKLGKRGKFIACTGFKEGCTY
TRNLESDASEPSEPVLSDEKCDKCGQQMLIKDGRYGKYLACSGYPACKNIQPLVKPRGTGVVCPECKEGELTEKKSRFGK
LFYSCNRYPQCKFALWDPPVEQPCPKCGFPLLVKKVYKRKGEFLKCPKEGCDYTSE
>Mature_775_residues
PQNLVIVESPAKAKTIEKFLGKDYKVLASFGHVRALPSKQGSVDIEQDFTPKYHILPESKKHIDAIKAALKDAKNLLLAT
DPDREGEAISWHLLAALGLDKKNPGKSIQRVVFHEITKDAIIHAVENPRDIATDLVDAQQARSVLDYLVGFNLSPFLWKK
IRYGLSAGRVQSVALRLVCEREKEIQAFKEQEYWTIAARLGVKPGAEFRASLVEVGGKKLGKFDIPDENVATALNRALKS
GSYRVAKITRTERKRNPSPPFTTSTLQQEASRKLGFSAKKTMSTAQKLYEGIDIGQEGTVGLITYMRTDSVTLSSQALTE
AHEVISAAYGAEYALAKPRIFKTKTKNAQEAHEAVRPTSIARTPAELKKHLTPDLFKLYELIWKRTVASQMAEALLDQTS
VDIAAELNEAVADCTVAESGAASPFTGAKRCGLRAAGTVIRFPGFMKLYIEGLDDQDEEKEGLLPPLQEGDDLKLHELFP
EQHFTQPPPRYTEATLVKTLEEYGIGRPSTYASIMNTLVERKYTRLDKKRFFPEDVGMVVNDLLVAHFSRYVDYNFTAGL
EEELDQVSRGEKQWKPLLKEFWEPFITLLKQKEGEVKKDDLTTTELDEVCPECGKPLAIKLGKRGKFIACTGFKEGCTYT
RNLESDASEPSEPVLSDEKCDKCGQQMLIKDGRYGKYLACSGYPACKNIQPLVKPRGTGVVCPECKEGELTEKKSRFGKL
FYSCNRYPQCKFALWDPPVEQPCPKCGFPLLVKKVYKRKGEFLKCPKEGCDYTSE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=778, Percent_Identity=22.6221079691517, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI10835218, Length=603, Percent_Identity=24.7097844112769, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787529, Length=809, Percent_Identity=37.3300370828183, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI48994931, Length=159, Percent_Identity=38.9937106918239, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1788061, Length=582, Percent_Identity=25.4295532646048, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=722, Percent_Identity=23.1301939058172, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=585, Percent_Identity=24.957264957265, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585251, Length=662, Percent_Identity=24.9244712990937, Blast_Score=124, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 86766; Mature: 86635

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNLVIVESPAKAKTIEKFLGKDYKVLASFGHVRALPSKQGSVDIEQDFTPKYHILPES
CCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEECCCC
KKHIDAIKAALKDAKNLLLATDPDREGEAISWHLLAALGLDKKNPGKSIQRVVFHEITKD
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIIHAVENPRDIATDLVDAQQARSVLDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLVC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIQAFKEQEYWTIAARLGVKPGAEFRASLVEVGGKKLGKFDIPDENVATALNRALK
HHHHHHHHHHHHHHEEEEHHHCCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHH
SGSYRVAKITRTERKRNPSPPFTTSTLQQEASRKLGFSAKKTMSTAQKLYEGIDIGQEGT
CCCEEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
VGLITYMRTDSVTLSSQALTEAHEVISAAYGAEYALAKPRIFKTKTKNAQEAHEAVRPTS
EEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCHHHHHHHHCCHH
IARTPAELKKHLTPDLFKLYELIWKRTVASQMAEALLDQTSVDIAAELNEAVADCTVAES
HHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAASPFTGAKRCGLRAAGTVIRFPGFMKLYIEGLDDQDEEKEGLLPPLQEGDDLKLHELF
CCCCCCCCHHHCCCHHCCCHHCCCCCEEHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHC
PEQHFTQPPPRYTEATLVKTLEEYGIGRPSTYASIMNTLVERKYTRLDKKRFFPEDVGMV
CHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VNDLLVAHFSRYVDYNFTAGLEEELDQVSRGEKQWKPLLKEFWEPFITLLKQKEGEVKKD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLTTTELDEVCPECGKPLAIKLGKRGKFIACTGFKEGCTYTRNLESDASEPSEPVLSDEK
CCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CDKCGQQMLIKDGRYGKYLACSGYPACKNIQPLVKPRGTGVVCPECKEGELTEKKSRFGK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCHHHHHHHHH
LFYSCNRYPQCKFALWDPPVEQPCPKCGFPLLVKKVYKRKGEFLKCPKEGCDYTSE
HHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCHHCCCCCCC
>Mature Secondary Structure 
PQNLVIVESPAKAKTIEKFLGKDYKVLASFGHVRALPSKQGSVDIEQDFTPKYHILPES
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCEEECCCC
KKHIDAIKAALKDAKNLLLATDPDREGEAISWHLLAALGLDKKNPGKSIQRVVFHEITKD
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AIIHAVENPRDIATDLVDAQQARSVLDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLVC
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIQAFKEQEYWTIAARLGVKPGAEFRASLVEVGGKKLGKFDIPDENVATALNRALK
HHHHHHHHHHHHHHEEEEHHHCCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHH
SGSYRVAKITRTERKRNPSPPFTTSTLQQEASRKLGFSAKKTMSTAQKLYEGIDIGQEGT
CCCEEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
VGLITYMRTDSVTLSSQALTEAHEVISAAYGAEYALAKPRIFKTKTKNAQEAHEAVRPTS
EEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCHHHHHHHHCCHH
IARTPAELKKHLTPDLFKLYELIWKRTVASQMAEALLDQTSVDIAAELNEAVADCTVAES
HHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAASPFTGAKRCGLRAAGTVIRFPGFMKLYIEGLDDQDEEKEGLLPPLQEGDDLKLHELF
CCCCCCCCHHHCCCHHCCCHHCCCCCEEHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHC
PEQHFTQPPPRYTEATLVKTLEEYGIGRPSTYASIMNTLVERKYTRLDKKRFFPEDVGMV
CHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VNDLLVAHFSRYVDYNFTAGLEEELDQVSRGEKQWKPLLKEFWEPFITLLKQKEGEVKKD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLTTTELDEVCPECGKPLAIKLGKRGKFIACTGFKEGCTYTRNLESDASEPSEPVLSDEK
CCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CDKCGQQMLIKDGRYGKYLACSGYPACKNIQPLVKPRGTGVVCPECKEGELTEKKSRFGK
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCHHHHHHHHH
LFYSCNRYPQCKFALWDPPVEQPCPKCGFPLLVKKVYKRKGEFLKCPKEGCDYTSE
HHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]