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The map label for this gene is hisZ [H]

Identifier: 118578958

GI number: 118578958

Start: 549495

End: 550832

Strand: Reverse

Name: hisZ [H]

Synonym: Ppro_0519

Alternate gene names: 118578958

Gene position: 550832-549495 (Counterclockwise)

Preceding gene: 118578959

Following gene: 118578957

Centisome position: 13.74

GC content: 64.2

Gene sequence:

>1338_bases
TTGCTCACATTCCGAGATTCCAACCCTATGAACAACAGACTTCCCGACATATCGCTTCCCTTCGGGGTGAGCGACTTTCT
CCCCGATGCCGCCGCCAAAATCGGCTACATCGAGGACAGAATCCGCCGCGTGTTCGAACTGTGGGGCTTCCGCAGAGTCA
TCACGCCCAAACTGGAATACGAGGACGTAATGGCTCTCGGCCTGGGTGACGGCCTCAAAAACAAGACCTACCGCTTTGAC
GACCGCCAGACCGGCAGACTGTTGGCCTTTCCTCCGGACATCACCCCGCAGATCGCCCGTATCGTGGCCACGCGCCTCGC
CGCCAGCCCCCTTCCACACCGCATCAGCTACAGCGGCCGGGTGTTGCGCCAGACCGAGAGCCAGACAGGACGCAGCCGCG
AGATTTTCCAGTCCGGCGTGGAACTGATCGGCCTGGACTCCCCCGAGGCGGACGCCGAAATGGTGGCCATGGCGGTGGAA
GCGCTGCAGGGGCTGGGCTTCAGCGACTTCAAGATCGACCTGGGTCAGGTTGAATTCTTCCGCGGCATCGTGGAATCTTC
AGGACTCAGCGGGCAACCGCTGCGGGATCTGCTGGAGGCGGTGGCGCGCAAGGACGCCTCGGCAGTGGCGCGCATCTGCG
CCGAAAGCCCGCTCTCCGAGGAATCACGCCGCGAGATCGCTTCCCTGCCGCGCCTGTTCGGCGGACGGGAGGTCGTTGAG
GCCGCCGACCGGGTAGCATGCAACAGCCGCTCCCGCGCCGCGCTGGACAACCTGCGCCAGGTGCTGGACATCCTGGACAT
CCACGGCGTGAGCGACTATCTGACCATCGATCTGGGCGAAACCCGCGGTCTGGACTACCACACCGGCATCACCTTCGAGG
GCTTCGTTACCGGCCTGGGCGTGCCGGTCTGCGGCGGCGGGCGCTACGACAGCCTGGCGGGTCGCTACGGATTCGAAACA
CCGGCCACCGGCTTCACCTTCGATGTGCTCAGCCTGCTCCAGGCCCTGGAGCGGCGTCCGGAACTGGAGGCCAGCACGGC
CCGCGATTTCCTGCTGTTCAACCGTGGCAGCGACCGGCGCCAGGCTCTGGAAACCGCTCGCCTGCTGCGCTCACGGGGAT
ACTCCACCGCCCGCGACATCATTCGGCGCGACGTCCAACCTTCGCTGGAGTATGCGCGCAGGATGAACATCCGCTGCATG
CTGGTCATGGGAGACGAATCGGACAAGGGTGTCGTGCGCGCGATTCGCACCCGTGACGGCAAGACGGTGCGCCTGGCCAG
TGACATGCTGTGCCGTGACGGACTGATGGAATATATTGAAGAAAACCAGGGAGAATAG

Upstream 100 bases:

>100_bases
AGAGACCCGGCCTGACCACACGGGGCACCGCTTCACTGGCCATCAAGAAGGTTGAATAACCGCTTCCCCTCAGCCCCCCA
AGCGGGGGCTGATTCGTGAG

Downstream 100 bases:

>100_bases
GCAAAATGGCAAACGTTGCGATTGTGGGCGCCCAGTGGGGCGATGAGGGAAAAGGGAAGGTCGTCGACATCTACACCGAA
TATGCCGATGATATCGTTCG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLTFRDSNPMNNRLPDISLPFGVSDFLPDAAAKIGYIEDRIRRVFELWGFRRVITPKLEYEDVMALGLGDGLKNKTYRFD
DRQTGRLLAFPPDITPQIARIVATRLAASPLPHRISYSGRVLRQTESQTGRSREIFQSGVELIGLDSPEADAEMVAMAVE
ALQGLGFSDFKIDLGQVEFFRGIVESSGLSGQPLRDLLEAVARKDASAVARICAESPLSEESRREIASLPRLFGGREVVE
AADRVACNSRSRAALDNLRQVLDILDIHGVSDYLTIDLGETRGLDYHTGITFEGFVTGLGVPVCGGGRYDSLAGRYGFET
PATGFTFDVLSLLQALERRPELEASTARDFLLFNRGSDRRQALETARLLRSRGYSTARDIIRRDVQPSLEYARRMNIRCM
LVMGDESDKGVVRAIRTRDGKTVRLASDMLCRDGLMEYIEENQGE

Sequences:

>Translated_445_residues
MLTFRDSNPMNNRLPDISLPFGVSDFLPDAAAKIGYIEDRIRRVFELWGFRRVITPKLEYEDVMALGLGDGLKNKTYRFD
DRQTGRLLAFPPDITPQIARIVATRLAASPLPHRISYSGRVLRQTESQTGRSREIFQSGVELIGLDSPEADAEMVAMAVE
ALQGLGFSDFKIDLGQVEFFRGIVESSGLSGQPLRDLLEAVARKDASAVARICAESPLSEESRREIASLPRLFGGREVVE
AADRVACNSRSRAALDNLRQVLDILDIHGVSDYLTIDLGETRGLDYHTGITFEGFVTGLGVPVCGGGRYDSLAGRYGFET
PATGFTFDVLSLLQALERRPELEASTARDFLLFNRGSDRRQALETARLLRSRGYSTARDIIRRDVQPSLEYARRMNIRCM
LVMGDESDKGVVRAIRTRDGKTVRLASDMLCRDGLMEYIEENQGE
>Mature_445_residues
MLTFRDSNPMNNRLPDISLPFGVSDFLPDAAAKIGYIEDRIRRVFELWGFRRVITPKLEYEDVMALGLGDGLKNKTYRFD
DRQTGRLLAFPPDITPQIARIVATRLAASPLPHRISYSGRVLRQTESQTGRSREIFQSGVELIGLDSPEADAEMVAMAVE
ALQGLGFSDFKIDLGQVEFFRGIVESSGLSGQPLRDLLEAVARKDASAVARICAESPLSEESRREIASLPRLFGGREVVE
AADRVACNSRSRAALDNLRQVLDILDIHGVSDYLTIDLGETRGLDYHTGITFEGFVTGLGVPVCGGGRYDSLAGRYGFET
PATGFTFDVLSLLQALERRPELEASTARDFLLFNRGSDRRQALETARLLRSRGYSTARDIIRRDVQPSLEYARRMNIRCM
LVMGDESDKGVVRAIRTRDGKTVRLASDMLCRDGLMEYIEENQGE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=460, Percent_Identity=25.4347826086956, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI15029520, Length=360, Percent_Identity=26.3888888888889, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=25.531914893617, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71993693, Length=453, Percent_Identity=24.0618101545254, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=453, Percent_Identity=24.0618101545254, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=339, Percent_Identity=26.5486725663717, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643061, Length=464, Percent_Identity=26.7241379310345, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24643059, Length=483, Percent_Identity=25.6728778467909, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 49543; Mature: 49543

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFRDSNPMNNRLPDISLPFGVSDFLPDAAAKIGYIEDRIRRVFELWGFRRVITPKLEY
CCEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCH
EDVMALGLGDGLKNKTYRFDDRQTGRLLAFPPDITPQIARIVATRLAASPLPHRISYSGR
HHHHHEECCCCCCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
VLRQTESQTGRSREIFQSGVELIGLDSPEADAEMVAMAVEALQGLGFSDFKIDLGQVEFF
HHHHHHHHCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
RGIVESSGLSGQPLRDLLEAVARKDASAVARICAESPLSEESRREIASLPRLFGGREVVE
HHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
AADRVACNSRSRAALDNLRQVLDILDIHGVSDYLTIDLGETRGLDYHTGITFEGFVTGLG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCC
VPVCGGGRYDSLAGRYGFETPATGFTFDVLSLLQALERRPELEASTARDFLLFNRGSDRR
CCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCCCHHH
QALETARLLRSRGYSTARDIIRRDVQPSLEYARRMNIRCMLVMGDESDKGVVRAIRTRDG
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC
KTVRLASDMLCRDGLMEYIEENQGE
CEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTFRDSNPMNNRLPDISLPFGVSDFLPDAAAKIGYIEDRIRRVFELWGFRRVITPKLEY
CCEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCH
EDVMALGLGDGLKNKTYRFDDRQTGRLLAFPPDITPQIARIVATRLAASPLPHRISYSGR
HHHHHEECCCCCCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
VLRQTESQTGRSREIFQSGVELIGLDSPEADAEMVAMAVEALQGLGFSDFKIDLGQVEFF
HHHHHHHHCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
RGIVESSGLSGQPLRDLLEAVARKDASAVARICAESPLSEESRREIASLPRLFGGREVVE
HHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
AADRVACNSRSRAALDNLRQVLDILDIHGVSDYLTIDLGETRGLDYHTGITFEGFVTGLG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCC
VPVCGGGRYDSLAGRYGFETPATGFTFDVLSLLQALERRPELEASTARDFLLFNRGSDRR
CCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCCCHHH
QALETARLLRSRGYSTARDIIRRDVQPSLEYARRMNIRCMLVMGDESDKGVVRAIRTRDG
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC
KTVRLASDMLCRDGLMEYIEENQGE
CEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA