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The map label for this gene is purA

Identifier: 118578957

GI number: 118578957

Start: 548197

End: 549489

Strand: Reverse

Name: purA

Synonym: Ppro_0518

Alternate gene names: 118578957

Gene position: 549489-548197 (Counterclockwise)

Preceding gene: 118578958

Following gene: 118578954

Centisome position: 13.71

GC content: 62.41

Gene sequence:

>1293_bases
ATGGCAAACGTTGCGATTGTGGGCGCCCAGTGGGGCGATGAGGGAAAAGGGAAGGTCGTCGACATCTACACCGAATATGC
CGATGATATCGTTCGTTTTCAGGGCGGCAACAATGCCGGTCACACGCTGGTGGTCGGCAATGAAAAGGTCGTTCTGCATC
TGATCCCCTCGGGCATTCTTCACGAAGGCAAGCGCTGCATCATCGGCAACGGAGTCGTGCTGGACCCGGAAGTATTCATC
AAGGAGATCGTCAAGCTCAAGGAATCGGGCCGCCTCACCGACGACTCCTGCCTGCTCCTGTCGGAATCGTTGCACATCAT
CATGCCCTATCACAAGCGCATCGATATCGCCCGCGAAGCCAGAAGCGGTGACCGCAAGATCGGCACCACCGGACGCGGCA
TCGGCCCCTGCTACGAGGACAAGATCGGCCGCCGCGGCATCCGCCTGATGGACCTGCTCAACAGAGATGTCTTTGCCCGC
CGCTTGAAGGAATTCCTGGTGGAGAAGAACTTCCTGCTGGAAAAGATGCTGGGAGAGGCGCCCTGCGACTACGACCAGAT
CCTGGAAGAGTACAGCGGCTACGCCGAGGTGCTGCGCGCCTACATGGCCGACACCGCGCTGATCATCAACAACGACCTCC
AGGCCGGCAAGAAGATCCTCTTCGAGGGGGCCCAGGGAACGCTTTTGGATGTGGACTTCGGCACCTATCCCTTCGTGACT
TCATCTTCCACCTGCGCTGGCGGCGTCTGCACCGGCTCCGGCGTCGGGCCGCGCCACATTCACGAGATCATCGGCATCTC
CAAGGCCTATGTCACCAGGGTTGGCAGCGGCCCCTTCCCCACTGAGCTGTTCGACGCGGACGGCGACCGGCTGCGTGACG
CGGGGGGGGAATTCGGCTCCACCACCGGCCGTCCCCGGCGCTGCGGCTGGTTCGACGCCATGGTGATCCGTTACGCGGTG
CGCACCAACGGCCTTACCGGCATCGCCCTGACCAAGCTGGACGTGCTCTCCGGCTTCGACAGCATCAAGATCTGCACCGG
CTATACCCTGGACGGAAAGGCGCTGGAGACCCTCCCTTCCGCACCGGAGGCCTTCGAGAACTGTCAGCCGGTGTATGAGG
AGCTGCCGGGGTGGAACGCGGATATCTCCGGAGCAAAGAGCTTCGAGGAACTGCCGGACAGCGCCCGCGCTTATGTCAAG
CGCCTGGAGGAACTGGCTGGCTGCCCCATCGTGCTGGTCTCCGTGGGCGCCCGCCGCGACCAGACCATCATCCTGCGCAA
CCCCTTCCTGTAG

Upstream 100 bases:

>100_bases
TTCGCACCCGTGACGGCAAGACGGTGCGCCTGGCCAGTGACATGCTGTGCCGTGACGGACTGATGGAATATATTGAAGAA
AACCAGGGAGAATAGGCAAA

Downstream 100 bases:

>100_bases
GCGACCTGAGCGCGCATCATCCAACCGACGCATCATGTAAAAAATCCCCCCGGAATAGTGTTCCGGGGGGATTTTTTTAC
GCCTTGGATGACCCGCCTCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANVAIVGAQWGDEGKGKVVDIYTEYADDIVRFQGGNNAGHTLVVGNEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFI
KEIVKLKESGRLTDDSCLLLSESLHIIMPYHKRIDIAREARSGDRKIGTTGRGIGPCYEDKIGRRGIRLMDLLNRDVFAR
RLKEFLVEKNFLLEKMLGEAPCDYDQILEEYSGYAEVLRAYMADTALIINNDLQAGKKILFEGAQGTLLDVDFGTYPFVT
SSSTCAGGVCTGSGVGPRHIHEIIGISKAYVTRVGSGPFPTELFDADGDRLRDAGGEFGSTTGRPRRCGWFDAMVIRYAV
RTNGLTGIALTKLDVLSGFDSIKICTGYTLDGKALETLPSAPEAFENCQPVYEELPGWNADISGAKSFEELPDSARAYVK
RLEELAGCPIVLVSVGARRDQTIILRNPFL

Sequences:

>Translated_430_residues
MANVAIVGAQWGDEGKGKVVDIYTEYADDIVRFQGGNNAGHTLVVGNEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFI
KEIVKLKESGRLTDDSCLLLSESLHIIMPYHKRIDIAREARSGDRKIGTTGRGIGPCYEDKIGRRGIRLMDLLNRDVFAR
RLKEFLVEKNFLLEKMLGEAPCDYDQILEEYSGYAEVLRAYMADTALIINNDLQAGKKILFEGAQGTLLDVDFGTYPFVT
SSSTCAGGVCTGSGVGPRHIHEIIGISKAYVTRVGSGPFPTELFDADGDRLRDAGGEFGSTTGRPRRCGWFDAMVIRYAV
RTNGLTGIALTKLDVLSGFDSIKICTGYTLDGKALETLPSAPEAFENCQPVYEELPGWNADISGAKSFEELPDSARAYVK
RLEELAGCPIVLVSVGARRDQTIILRNPFL
>Mature_429_residues
ANVAIVGAQWGDEGKGKVVDIYTEYADDIVRFQGGNNAGHTLVVGNEKVVLHLIPSGILHEGKRCIIGNGVVLDPEVFIK
EIVKLKESGRLTDDSCLLLSESLHIIMPYHKRIDIAREARSGDRKIGTTGRGIGPCYEDKIGRRGIRLMDLLNRDVFARR
LKEFLVEKNFLLEKMLGEAPCDYDQILEEYSGYAEVLRAYMADTALIINNDLQAGKKILFEGAQGTLLDVDFGTYPFVTS
SSTCAGGVCTGSGVGPRHIHEIIGISKAYVTRVGSGPFPTELFDADGDRLRDAGGEFGSTTGRPRRCGWFDAMVIRYAVR
TNGLTGIALTKLDVLSGFDSIKICTGYTLDGKALETLPSAPEAFENCQPVYEELPGWNADISGAKSFEELPDSARAYVKR
LEELAGCPIVLVSVGARRDQTIILRNPFL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=42.9577464788732, Blast_Score=341, Evalue=9e-94,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=41.92037470726, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=41.2060301507538, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=51.1682242990654, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=43.2242990654206, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=43.2242990654206, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=42.4593967517401, Blast_Score=352, Evalue=5e-98,
Organism=Drosophila melanogaster, GI21356033, Length=420, Percent_Identity=41.1904761904762, Blast_Score=330, Evalue=9e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PELPD (A1ALC9)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900207.1
- ProteinModelPortal:   A1ALC9
- SMR:   A1ALC9
- STRING:   A1ALC9
- GeneID:   4572889
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0518
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVQPMK
- PhylomeDB:   A1ALC9
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46971; Mature: 46840

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVAIVGAQWGDEGKGKVVDIYTEYADDIVRFQGGNNAGHTLVVGNEKVVLHLIPSGIL
CCCEEEEECCCCCCCCCCEEEEEHHHHHHHEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCIIGNGVVLDPEVFIKEIVKLKESGRLTDDSCLLLSESLHIIMPYHKRIDIAREA
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECHHHHHHHHHHH
RSGDRKIGTTGRGIGPCYEDKIGRRGIRLMDLLNRDVFARRLKEFLVEKNFLLEKMLGEA
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PCDYDQILEEYSGYAEVLRAYMADTALIINNDLQAGKKILFEGAQGTLLDVDFGTYPFVT
CCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCHHHCCHHEEECCCCCEEEECCCCCCCEE
SSSTCAGGVCTGSGVGPRHIHEIIGISKAYVTRVGSGPFPTELFDADGDRLRDAGGEFGS
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHCCCCCHHHHHCCCCCCC
TTGRPRRCGWFDAMVIRYAVRTNGLTGIALTKLDVLSGFDSIKICTGYTLDGKALETLPS
CCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCC
APEAFENCQPVYEELPGWNADISGAKSFEELPDSARAYVKRLEELAGCPIVLVSVGARRD
CHHHHHHCCHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
QTIILRNPFL
CEEEEECCCC
>Mature Secondary Structure 
ANVAIVGAQWGDEGKGKVVDIYTEYADDIVRFQGGNNAGHTLVVGNEKVVLHLIPSGIL
CCEEEEECCCCCCCCCCEEEEEHHHHHHHEEECCCCCCCCEEEECCCEEEEEECCCHHH
HEGKRCIIGNGVVLDPEVFIKEIVKLKESGRLTDDSCLLLSESLHIIMPYHKRIDIAREA
HCCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECHHHHHHHHHHH
RSGDRKIGTTGRGIGPCYEDKIGRRGIRLMDLLNRDVFARRLKEFLVEKNFLLEKMLGEA
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PCDYDQILEEYSGYAEVLRAYMADTALIINNDLQAGKKILFEGAQGTLLDVDFGTYPFVT
CCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCHHHCCHHEEECCCCCEEEECCCCCCCEE
SSSTCAGGVCTGSGVGPRHIHEIIGISKAYVTRVGSGPFPTELFDADGDRLRDAGGEFGS
CCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHCCCCCHHHHHCCCCCCC
TTGRPRRCGWFDAMVIRYAVRTNGLTGIALTKLDVLSGFDSIKICTGYTLDGKALETLPS
CCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCC
APEAFENCQPVYEELPGWNADISGAKSFEELPDSARAYVKRLEELAGCPIVLVSVGARRD
CHHHHHHCCHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
QTIILRNPFL
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA