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The map label for this gene is nfo [H]

Identifier: 118578893

GI number: 118578893

Start: 476581

End: 477432

Strand: Reverse

Name: nfo [H]

Synonym: Ppro_0453

Alternate gene names: 118578893

Gene position: 477432-476581 (Counterclockwise)

Preceding gene: 118578894

Following gene: 118578889

Centisome position: 11.91

GC content: 62.21

Gene sequence:

>852_bases
ATGCCTGATTATCTTGGCGCCCACATGTCCATAGCAGGCGGCCTGCACCGGGCCATTGACCGCGCCATGGCGACCGGTTG
CGGCGTGCTGCAGATCTTTACCCGCAACTCCAATCAGTGGAAGGAAAAGACGCTGAGTGATGCCGATGTGGCCCTGTTCA
GGGAGAAATTCGCGGCTTCCGGCCTGCGCGAGGTGATCTCCCACGACATCTACCTGATCAACCTGGCTTCCGCTCCGGGG
GAGGTTCGCGACAAGAGCCTGGCGGCCTTCCGGGACGAACTAGAGACCTGTGCCCGACTAGGCATCGACAGGGTGGTCAT
GCACCCCGGATCGCATCTGTCCGACAGTCCGGAAGCTGCTCTGACGCGGGTGAGCCAGGCCTTCGACCGGCTCTTCCAGG
AGGTGCCGCTCTTCCAGGGAAGGGTGCTGCTGGAGACAACCGCCGGCCAGGGGACCAACCTGGGGCGGAACTTTCAGGAG
CTGCGGACTATTATCGACGGTTGCCGTTATCCGGAGCGTTTCGGGGTCTGCTTCGACACCTGCCACACCTTCGCCGCCGG
CTACGACATCACCAGCGAAGAGGGGTACCGCTCCACCAAGGAAGAATTCGACCAAATCATCGGGCTCGACCGACTGGAAT
GCTTCCACTTCAACGACTCCAGGAAAGGGCTCGGCTCACGGGTGGACCGCCACGAGCACATAGGCCAGGGCATGCTGGGG
CTCAACCCCTTCCGTTTCATCTTGAACGACCAGCGCTTCGCCACGACGCCCAAGATCCTGGAAACCCCCAAGGGGGACAA
TGACGAGATGGACGGGATCAACCTGGGGCTCCTGCGGGGGCTGGTGACCTGA

Upstream 100 bases:

>100_bases
GCAGGAGACCAGGTGGAGGTACACCTCTGGGGAAACCGCTCCGACCTGATCTGAGGCGAAGCAACCGGAATGAAAAATCA
CGTGAATACAGAGGTGAGCA

Downstream 100 bases:

>100_bases
GACGCCGTACGCGCAGCACACACAAACAGGCCGGGGCAGTCCCGGCCTGTTTGTGTGTATTCTCACCTGCCTTGGAAATA
CGGTCACATATTCCCGATCA

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPDYLGAHMSIAGGLHRAIDRAMATGCGVLQIFTRNSNQWKEKTLSDADVALFREKFAASGLREVISHDIYLINLASAPG
EVRDKSLAAFRDELETCARLGIDRVVMHPGSHLSDSPEAALTRVSQAFDRLFQEVPLFQGRVLLETTAGQGTNLGRNFQE
LRTIIDGCRYPERFGVCFDTCHTFAAGYDITSEEGYRSTKEEFDQIIGLDRLECFHFNDSRKGLGSRVDRHEHIGQGMLG
LNPFRFILNDQRFATTPKILETPKGDNDEMDGINLGLLRGLVT

Sequences:

>Translated_283_residues
MPDYLGAHMSIAGGLHRAIDRAMATGCGVLQIFTRNSNQWKEKTLSDADVALFREKFAASGLREVISHDIYLINLASAPG
EVRDKSLAAFRDELETCARLGIDRVVMHPGSHLSDSPEAALTRVSQAFDRLFQEVPLFQGRVLLETTAGQGTNLGRNFQE
LRTIIDGCRYPERFGVCFDTCHTFAAGYDITSEEGYRSTKEEFDQIIGLDRLECFHFNDSRKGLGSRVDRHEHIGQGMLG
LNPFRFILNDQRFATTPKILETPKGDNDEMDGINLGLLRGLVT
>Mature_282_residues
PDYLGAHMSIAGGLHRAIDRAMATGCGVLQIFTRNSNQWKEKTLSDADVALFREKFAASGLREVISHDIYLINLASAPGE
VRDKSLAAFRDELETCARLGIDRVVMHPGSHLSDSPEAALTRVSQAFDRLFQEVPLFQGRVLLETTAGQGTNLGRNFQEL
RTIIDGCRYPERFGVCFDTCHTFAAGYDITSEEGYRSTKEEFDQIIGLDRLECFHFNDSRKGLGSRVDRHEHIGQGMLGL
NPFRFILNDQRFATTPKILETPKGDNDEMDGINLGLLRGLVT

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=268, Percent_Identity=40.2985074626866, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=38.4328358208955, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6322735, Length=280, Percent_Identity=35.3571428571429, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31556; Mature: 31425

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDYLGAHMSIAGGLHRAIDRAMATGCGVLQIFTRNSNQWKEKTLSDADVALFREKFAAS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHH
GLREVISHDIYLINLASAPGEVRDKSLAAFRDELETCARLGIDRVVMHPGSHLSDSPEAA
HHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHH
LTRVSQAFDRLFQEVPLFQGRVLLETTAGQGTNLGRNFQELRTIIDGCRYPERFGVCFDT
HHHHHHHHHHHHHHCCHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
CHTFAAGYDITSEEGYRSTKEEFDQIIGLDRLECFHFNDSRKGLGSRVDRHEHIGQGMLG
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCC
LNPFRFILNDQRFATTPKILETPKGDNDEMDGINLGLLRGLVT
CCHHHHHCCCCHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PDYLGAHMSIAGGLHRAIDRAMATGCGVLQIFTRNSNQWKEKTLSDADVALFREKFAAS
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHH
GLREVISHDIYLINLASAPGEVRDKSLAAFRDELETCARLGIDRVVMHPGSHLSDSPEAA
HHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHH
LTRVSQAFDRLFQEVPLFQGRVLLETTAGQGTNLGRNFQELRTIIDGCRYPERFGVCFDT
HHHHHHHHHHHHHHCCHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
CHTFAAGYDITSEEGYRSTKEEFDQIIGLDRLECFHFNDSRKGLGSRVDRHEHIGQGMLG
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHCCCCCC
LNPFRFILNDQRFATTPKILETPKGDNDEMDGINLGLLRGLVT
CCHHHHHCCCCHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA