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The map label for this gene is rihA [H]

Identifier: 117921913

GI number: 117921913

Start: 4170160

End: 4171116

Strand: Direct

Name: rihA [H]

Synonym: Shewana3_3476

Alternate gene names: 117921913

Gene position: 4170160-4171116 (Clockwise)

Preceding gene: 117921907

Following gene: 117921914

Centisome position: 83.87

GC content: 53.29

Gene sequence:

>957_bases
ATGAGTCGTCCGATTATTTTGGATTGCGATCCCGGCCATGACGATGCTATCGCGCTCATTTTAGCCCTCGCCCATCCAGA
GCTAACCCCACTGGCCGTAACCACCAGCGCCGGCAACCAAACCCCAGATAAAACCCTCAATAACGCCCTGCGGATCTTAA
CCCTGCTCAATCGCAGCGATATTCCGGTCGCGGGCGGCGCGGTTAAACCCTTAAGCCGTGAGTTGATGATTGCCGATAAC
GTTCACGGTGAAACAGGGCTCGATGGCCCGGCATTGCCTGCACCGAGTTTTCAACCACAGGCCGTGAATGCGGTCGAGCT
AATGGCCGAGAAAATCCGCCAAAGCGATAAACCAGTAACATTAGTGCCTACTGGGCCGCTCACCAATATCGCGCTGCTAC
TCGCCAGCCATGCCGAGTTACACGCCAAAATTGAGCGCATTGTGTTGATGGGTGGTGCCGCCGGGGTTGGTAACTGGACA
CCCGCCGCCGAGTTTAATATTTTTGTCGACCCAGAGGCGGCCGACATTGTCTTTAAATCCGGTATTCCCATCACCATGTG
CGGCCTCGATGTTACCCATCAGGCACAGATCATGGATGAAGATATCGAACGTATTCGCGCTATTGCTAATCCCGTGGCTA
AGTGTGTCGCCGAGTTGTTGGATTTCTTTATGATTTACCATAGAGATCCTAAATGGGGCTTTGTCGGCGCGCCGCTGCAT
GACCCTTGCACTATCGCTTGGTTACTCAATCCCGCGCTGTTTGATGCCCAAGACTGCTGGGTCGGCATTGAAACCCAAAG
CGAGTTAACACTGGGGATGACGGTGGTCGACCGCTATCAACTAACGGGCAAACCCGCGAATGCGACAGTGCTCTTCGGCA
TTGATAGACAGGGCTTTGTTGACTTGTTAGTTGACAGTTTAGCGGTCTACACACCAACCTATCTCAATCGCAGATGA

Upstream 100 bases:

>100_bases
AAAGCTCACAGTTTGCCCTAAAGACTCGGCCACATCCTAGCCTGCAAACTTGAGCATGCTATTCTACGTTTCCATTCCCG
CTGTTTAAAGGAGTAACAGT

Downstream 100 bases:

>100_bases
ACTTAAGGTAACGCTTCCATGAGCCACATACTCGTTATAGGCAGTGCCAACGCCGATCATGTTATGAATTTTGAGTATTT
GCCCGTTCCCGGCCAAACCT

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSRPIILDCDPGHDDAIALILALAHPELTPLAVTTSAGNQTPDKTLNNALRILTLLNRSDIPVAGGAVKPLSRELMIADN
VHGETGLDGPALPAPSFQPQAVNAVELMAEKIRQSDKPVTLVPTGPLTNIALLLASHAELHAKIERIVLMGGAAGVGNWT
PAAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDEDIERIRAIANPVAKCVAELLDFFMIYHRDPKWGFVGAPLH
DPCTIAWLLNPALFDAQDCWVGIETQSELTLGMTVVDRYQLTGKPANATVLFGIDRQGFVDLLVDSLAVYTPTYLNRR

Sequences:

>Translated_318_residues
MSRPIILDCDPGHDDAIALILALAHPELTPLAVTTSAGNQTPDKTLNNALRILTLLNRSDIPVAGGAVKPLSRELMIADN
VHGETGLDGPALPAPSFQPQAVNAVELMAEKIRQSDKPVTLVPTGPLTNIALLLASHAELHAKIERIVLMGGAAGVGNWT
PAAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDEDIERIRAIANPVAKCVAELLDFFMIYHRDPKWGFVGAPLH
DPCTIAWLLNPALFDAQDCWVGIETQSELTLGMTVVDRYQLTGKPANATVLFGIDRQGFVDLLVDSLAVYTPTYLNRR
>Mature_317_residues
SRPIILDCDPGHDDAIALILALAHPELTPLAVTTSAGNQTPDKTLNNALRILTLLNRSDIPVAGGAVKPLSRELMIADNV
HGETGLDGPALPAPSFQPQAVNAVELMAEKIRQSDKPVTLVPTGPLTNIALLLASHAELHAKIERIVLMGGAAGVGNWTP
AAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDEDIERIRAIANPVAKCVAELLDFFMIYHRDPKWGFVGAPLHD
PCTIAWLLNPALFDAQDCWVGIETQSELTLGMTVVDRYQLTGKPANATVLFGIDRQGFVDLLVDSLAVYTPTYLNRR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=72.258064516129, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1788486, Length=313, Percent_Identity=42.1725239616613, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1786213, Length=311, Percent_Identity=43.4083601286174, Blast_Score=214, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI32565474, Length=194, Percent_Identity=33.5051546391753, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17565698, Length=195, Percent_Identity=34.3589743589744, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=36.1867704280156, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24641839, Length=194, Percent_Identity=37.1134020618557, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583915, Length=189, Percent_Identity=35.978835978836, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24641837, Length=196, Percent_Identity=36.2244897959184, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34289; Mature: 34158

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPIILDCDPGHDDAIALILALAHPELTPLAVTTSAGNQTPDKTLNNALRILTLLNRSD
CCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IPVAGGAVKPLSRELMIADNVHGETGLDGPALPAPSFQPQAVNAVELMAEKIRQSDKPVT
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LVPTGPLTNIALLLASHAELHAKIERIVLMGGAAGVGNWTPAAEFNIFVDPEAADIVFKS
EEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEC
GIPITMCGLDVTHQAQIMDEDIERIRAIANPVAKCVAELLDFFMIYHRDPKWGFVGAPLH
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCC
DPCTIAWLLNPALFDAQDCWVGIETQSELTLGMTVVDRYQLTGKPANATVLFGIDRQGFV
CCEEEEEEECCHHCCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCHH
DLLVDSLAVYTPTYLNRR
HHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SRPIILDCDPGHDDAIALILALAHPELTPLAVTTSAGNQTPDKTLNNALRILTLLNRSD
CCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IPVAGGAVKPLSRELMIADNVHGETGLDGPALPAPSFQPQAVNAVELMAEKIRQSDKPVT
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LVPTGPLTNIALLLASHAELHAKIERIVLMGGAAGVGNWTPAAEFNIFVDPEAADIVFKS
EEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEC
GIPITMCGLDVTHQAQIMDEDIERIRAIANPVAKCVAELLDFFMIYHRDPKWGFVGAPLH
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCC
DPCTIAWLLNPALFDAQDCWVGIETQSELTLGMTVVDRYQLTGKPANATVLFGIDRQGFV
CCEEEEEEECCHHCCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCHH
DLLVDSLAVYTPTYLNRR
HHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA