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The map label for this gene is pcnB [H]

Identifier: 117921843

GI number: 117921843

Start: 4074921

End: 4076228

Strand: Direct

Name: pcnB [H]

Synonym: Shewana3_3406

Alternate gene names: 117921843

Gene position: 4074921-4076228 (Clockwise)

Preceding gene: 117921842

Following gene: 117921844

Centisome position: 81.95

GC content: 52.83

Gene sequence:

>1308_bases
GTGCCTCGGGATGCGCATTCGATCTCCCGTAAGCAGATCAGCGAAAACGCACTTAAAGTATTATACCGATTGAATAAATC
AGGTTTCCAGGCCTATTTAGTTGGTGGGGGCGTACGGGATTTACTCCTCGGACTCGAACCGAAAGACTTCGACGTGGTCA
CCAATGCGACGCCGGAAGAGATTAAAAAGCTGTTTCGCAATTGCCGCGTAGTCGGACGCCGCTTCCGCCTCGCCCATATT
GTGTTTGGCCGCGATGTGATTGAAGTTGCCACCTTCCGTGGTCACCACGGCGACAGTAATGAAAAGATTTCCAAAGCCAA
TGCCGAAGGGCGTTTGCTCAGGGATAACGTGTACGGTGAAATCGATGAAGACGCAGAGCGCCGCGATTTTACCGTCAACG
CCCTTTACTACGATATTAGCGACTACTCTATCCGTAGCTATGGCGGCGGTATGGAAGATCTTAAGGCGGGCACCTTAAGG
CTGATTGGCGATCCCGAAACCCGTTACCGTGAAGATCCAGTGCGTATGCTCAGGGCTGTGCGTTTTGCCACTAAGCTCAG
CATGGCTATTGAAGAAGTGACCGCTAAACCTATCAAACAGTTAGCGCCACTGCTCAAGGACATTCCAGCCGCCCGCATGT
ATGAAGAAGTGCTCAAACTTTTCTTCGCCGGCAAAGCCATGGCCAACTTTGAGATGATGCAGGAATATAAGCTGTTCGCG
CCGCTCTTCCCGCAAGTCGACGCCCAATTAAAAGATGCGCCAAAAGGCCCTGCGATGAAGATGGTGCAAGCCATTATGAA
GAGCACGGATGCGCGCGTGAACGAAGATAAGCCTGTTACTCCGTCATTCTTCTATGCGGCGATCCTCTGGTATCCACTAC
GTCAACGCGCCGAAGATATCGCGGTCGAGAGCGGTCTGACTTTATACGATGCGTTTTTTGCCGCCATGGGTGATGTGATT
GAGCAGCAATGCCAAACCATCAGCATTCCGCGCCGTTTTAGTACGCCCGCCAAAGACATCTGGCAATTGCAATTACGCTT
CGATCGCAGCCAAGGCACACGCGCCTTTAAACTGTTAGAGCACCCTAAATTTAGAGCGGCCTACGACTTACTGTTACTGC
GCGGCGAAGTCGAAGGTGGCAATGTCGCCAAGAGTGCCGCCTGGTGGCAACAGTTTGTTGAAGCGGATGAAACCGAGCGT
TTGAACATCGCCCGCAGTGGTAACAAAGCGGCGGGTAACCGTAACCGCAATTCACCACAGCGCCGTCGCCGTCGCCCGAG
TGCCGCTAAAGCCAAAGCCGCCGAATAA

Upstream 100 bases:

>100_bases
GCAGCTATTTGAAGACGCGCCTAAAACGACCGCTCCGACTCCAGCTCAGATTGAACCCCAAACGCCAGAACTCACTTCAG
GCGGCTTAAGCTTATCGCTC

Downstream 100 bases:

>100_bases
TGACTCAGGTTTTTGTCGCCCTCGGGGCTAATCTTGAAGATCCTAAAGCTCAGCTGGACAATGCCGTTGCGGCCTTGTCC
GCCTTGGCTGAACATCAGAG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPRDAHSISRKQISENALKVLYRLNKSGFQAYLVGGGVRDLLLGLEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHI
VFGRDVIEVATFRGHHGDSNEKISKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLR
LIGDPETRYREDPVRMLRAVRFATKLSMAIEEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAMANFEMMQEYKLFA
PLFPQVDAQLKDAPKGPAMKMVQAIMKSTDARVNEDKPVTPSFFYAAILWYPLRQRAEDIAVESGLTLYDAFFAAMGDVI
EQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLLEHPKFRAAYDLLLLRGEVEGGNVAKSAAWWQQFVEADETER
LNIARSGNKAAGNRNRNSPQRRRRRPSAAKAKAAE

Sequences:

>Translated_435_residues
MPRDAHSISRKQISENALKVLYRLNKSGFQAYLVGGGVRDLLLGLEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHI
VFGRDVIEVATFRGHHGDSNEKISKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLR
LIGDPETRYREDPVRMLRAVRFATKLSMAIEEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAMANFEMMQEYKLFA
PLFPQVDAQLKDAPKGPAMKMVQAIMKSTDARVNEDKPVTPSFFYAAILWYPLRQRAEDIAVESGLTLYDAFFAAMGDVI
EQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLLEHPKFRAAYDLLLLRGEVEGGNVAKSAAWWQQFVEADETER
LNIARSGNKAAGNRNRNSPQRRRRRPSAAKAKAAE
>Mature_434_residues
PRDAHSISRKQISENALKVLYRLNKSGFQAYLVGGGVRDLLLGLEPKDFDVVTNATPEEIKKLFRNCRVVGRRFRLAHIV
FGRDVIEVATFRGHHGDSNEKISKANAEGRLLRDNVYGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLRL
IGDPETRYREDPVRMLRAVRFATKLSMAIEEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAMANFEMMQEYKLFAP
LFPQVDAQLKDAPKGPAMKMVQAIMKSTDARVNEDKPVTPSFFYAAILWYPLRQRAEDIAVESGLTLYDAFFAAMGDVIE
QQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLLEHPKFRAAYDLLLLRGEVEGGNVAKSAAWWQQFVEADETERL
NIARSGNKAAGNRNRNSPQRRRRRPSAAKAKAAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=166, Percent_Identity=30.7228915662651, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081691, Length=395, Percent_Identity=58.7341772151899, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1789436, Length=226, Percent_Identity=29.646017699115, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=203, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281360112, Length=290, Percent_Identity=26.551724137931, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357337, Length=290, Percent_Identity=26.551724137931, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 49391; Mature: 49260

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRDAHSISRKQISENALKVLYRLNKSGFQAYLVGGGVRDLLLGLEPKDFDVVTNATPEE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHH
IKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGDSNEKISKANAEGRLLRDNVYGE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCCCCC
IDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCCHHHCCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHH
RFATKLSMAIEEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAMANFEMMQEYKLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLFPQVDAQLKDAPKGPAMKMVQAIMKSTDARVNEDKPVTPSFFYAAILWYPLRQRAEDI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AVESGLTLYDAFFAAMGDVIEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLLE
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHHHHH
HPKFRAAYDLLLLRGEVEGGNVAKSAAWWQQFVEADETERLNIARSGNKAAGNRNRNSPQ
CCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHH
RRRRRPSAAKAKAAE
HHHCCCCHHHCCCCC
>Mature Secondary Structure 
PRDAHSISRKQISENALKVLYRLNKSGFQAYLVGGGVRDLLLGLEPKDFDVVTNATPEE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHH
IKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGDSNEKISKANAEGRLLRDNVYGE
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEECCCCCC
IDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLRLIGDPETRYREDPVRMLRAV
CCCCCCCCCEEEEEEEEECCCCCHHHCCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHH
RFATKLSMAIEEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAMANFEMMQEYKLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLFPQVDAQLKDAPKGPAMKMVQAIMKSTDARVNEDKPVTPSFFYAAILWYPLRQRAEDI
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AVESGLTLYDAFFAAMGDVIEQQCQTISIPRRFSTPAKDIWQLQLRFDRSQGTRAFKLLE
HHHCCCHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHHHHEECCCCCCHHHHHHH
HPKFRAAYDLLLLRGEVEGGNVAKSAAWWQQFVEADETERLNIARSGNKAAGNRNRNSPQ
CCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHH
RRRRRPSAAKAKAAE
HHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]