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The map label for this gene is tyrS [H]

Identifier: 117921496

GI number: 117921496

Start: 3664316

End: 3665512

Strand: Reverse

Name: tyrS [H]

Synonym: Shewana3_3058

Alternate gene names: 117921496

Gene position: 3665512-3664316 (Counterclockwise)

Preceding gene: 117921499

Following gene: 117921487

Centisome position: 73.72

GC content: 47.54

Gene sequence:

>1197_bases
ATGGCTGATTTAGACCAAGTATTAGCAGAAATTAGACGTGGTACCGATGAGATCCTACTCGAATCGGATCTGCTGGAAAA
ACTCAAAGAAGGACGTCCACTTCGTATCAAGTTAGGCGCTGATCCTACCGCACCGGATATCCATCTTGGACACACAGTAA
TTTTAAATAAATTAAGATTATTCCAAGAGTTAGGTCATGAAGTCATTTTCTTAATTGGTGACTTTACCGGTATGGTGGGT
GATCCAAGTGGTAAAAACAGTACACGTCCACCTCTCACCCGTGAGCAAGTATTAGCCAACGCCGAGACCTATAAAGAGCA
GGTCTATAAAATTTTGGATCCTGCCAAAACCCGTATCGAGTTCAACTCGAGCTGGTTAGAGCCATTAGGCGCAGCAGGGA
TGATCCGTTTAGCCTCGCAGCAAACCGTTGCCCGTATGATGGAACGTGACGACTTTAAGAAGCGTTATGCTTCGGGTCAG
TCGATCGCTATCCACGAATTTATGTATCCACTGCTACAGGGTTATGACTCAGTAGCTCTGAAGGCCGACGTTGAATTAGG
CGGAACCGATCAGAAGTTCAACCTGTTGATGGGCCGTGAGTTACAAAAAGCCGAAGGCCAAAAGCCACAAGCCGTGATTA
TGATGCCACTGCTTGAAGGTTTAGACGGCGTGAAGAAAATGTCTAAATCGGCGCACAACTACATTGGTGTGAGTGAGCCC
ACCAATGAAATGTTTGGCAAGATCATGTCAATTTCTGACGAATTAATGTGGCGCTACTTTGAGTTGCTGTCATTCCGTCC
TTTGGCCGAGATTGAACAGTTCAAACAAGATATCGCCAACGGTGCTAACCCCCGTGATACCAAGATTGCCCTTGCGAAGG
AAATCATCGCGCGCTTCCACGACCAAGCGGCGGCCGAGAGTGCCCATCAAGCCTTTATCGACCGTTTCCAAAAGGGTGCT
ATCCCAGATGATATCCCTGAGGTTGAATTAGCGGCGGGTGAAGGCTTAGCGATTGCTAACTTACTTAAAGATGCCGACTT
AGTGGGTTCGACTTCCGATGCGATGCGTATGATCAAGCAAGGCGCGGTGAAGATGGACGGTGAGAAGGTTGATGATAGCC
GTATGACCTTAAGCGCTGGTACGGTTGCCGTATTCCAAGTCGGTAAGCGTAAGTTCGCGAAAGTGACTCTGGTTTAA

Upstream 100 bases:

>100_bases
TTGAATAATCCCGTTAGTGTACACACTTTCAATTGTGGTGGCTAACAAGTAACATAAGTCTCCTCATTTTATGATCATGG
CCTTAGGAGTCCCCGCCGAG

Downstream 100 bases:

>100_bases
GCCGAATCGGATGTCACCATGTGAGTACCGCTTGTGAGTGCTTAAGACGGTTAATAAGTACTTACCAAGCAAGTAAAAAA
GAGCGCCTAAGGGCGCTCTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MADLDQVLAEIRRGTDEILLESDLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEPLGAAGMIRLASQQTVARMMERDDFKKRYASGQ
SIAIHEFMYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
TNEMFGKIMSISDELMWRYFELLSFRPLAEIEQFKQDIANGANPRDTKIALAKEIIARFHDQAAAESAHQAFIDRFQKGA
IPDDIPEVELAAGEGLAIANLLKDADLVGSTSDAMRMIKQGAVKMDGEKVDDSRMTLSAGTVAVFQVGKRKFAKVTLV

Sequences:

>Translated_398_residues
MADLDQVLAEIRRGTDEILLESDLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEPLGAAGMIRLASQQTVARMMERDDFKKRYASGQ
SIAIHEFMYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
TNEMFGKIMSISDELMWRYFELLSFRPLAEIEQFKQDIANGANPRDTKIALAKEIIARFHDQAAAESAHQAFIDRFQKGA
IPDDIPEVELAAGEGLAIANLLKDADLVGSTSDAMRMIKQGAVKMDGEKVDDSRMTLSAGTVAVFQVGKRKFAKVTLV
>Mature_397_residues
ADLDQVLAEIRRGTDEILLESDLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVGD
PSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEPLGAAGMIRLASQQTVARMMERDDFKKRYASGQS
IAIHEFMYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEPT
NEMFGKIMSISDELMWRYFELLSFRPLAEIEQFKQDIANGANPRDTKIALAKEIIARFHDQAAAESAHQAFIDRFQKGAI
PDDIPEVELAAGEGLAIANLLKDADLVGSTSDAMRMIKQGAVKMDGEKVDDSRMTLSAGTVAVFQVGKRKFAKVTLV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=415, Percent_Identity=25.7831325301205, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1787925, Length=402, Percent_Identity=26.6169154228856, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=385, Percent_Identity=28.051948051948, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=304, Percent_Identity=25.3289473684211, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=23.8213399503722, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44104; Mature: 43973

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLDQVLAEIRRGTDEILLESDLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSSWLEPLGAAGMIRLASQQTVARMMERDDFKKRYASGQSIAIHEFMYPLLQGYDSVAL
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EEECEECCCCCHHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
TNEMFGKIMSISDELMWRYFELLSFRPLAEIEQFKQDIANGANPRDTKIALAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DQAAAESAHQAFIDRFQKGAIPDDIPEVELAAGEGLAIANLLKDADLVGSTSDAMRMIKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
GAVKMDGEKVDDSRMTLSAGTVAVFQVGKRKFAKVTLV
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEECC
>Mature Secondary Structure 
ADLDQVLAEIRRGTDEILLESDLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEE
FNSSWLEPLGAAGMIRLASQQTVARMMERDDFKKRYASGQSIAIHEFMYPLLQGYDSVAL
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EEECEECCCCCHHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
TNEMFGKIMSISDELMWRYFELLSFRPLAEIEQFKQDIANGANPRDTKIALAKEIIARFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DQAAAESAHQAFIDRFQKGAIPDDIPEVELAAGEGLAIANLLKDADLVGSTSDAMRMIKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
GAVKMDGEKVDDSRMTLSAGTVAVFQVGKRKFAKVTLV
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]