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The map label for this gene is phoB [H]

Identifier: 117921320

GI number: 117921320

Start: 3443625

End: 3444314

Strand: Reverse

Name: phoB [H]

Synonym: Shewana3_2879

Alternate gene names: 117921320

Gene position: 3444314-3443625 (Counterclockwise)

Preceding gene: 117921321

Following gene: 117921319

Centisome position: 69.27

GC content: 49.13

Gene sequence:

>690_bases
ATGACTGCAAGGATATTGATAGTAGAAGACGAGTTAGCCATCCGCGAGATGCTGACTTTTGTAATGGAACAGCATGGGTT
TACAACCTCTGCGGCGGAAGATTTTGATTCGGCCATTGCGCTGCTAAAGGAGCCTTACCCCGATCTGATTTTGTTGGATT
GGATGTTTCCTGGTGGAAGTGGTATTCAATTGGCCAAACGACTCAAGCAGGATGAGTTTACCCGTCAGATCCCCATTATT
ATGTTAACCGCTCGCGGTGAAGAAGAAGATAAGGTTAAGGGTCTCGAAGTGGGCGCCGATGACTATATCACTAAACCTTT
TTCACCCAAGGAGCTGGTGGCGCGTATTAAGGCTGTGTTGCGCCGTAGCGCGCCGACTCGCCTCGAAGAAACCATCGATG
TACAAGGATTGTTACTCGACCCAGTGAGCCACAGGGTGAGTGTTGGCGATACCGTACTCGATATGGGGCCAACGGAATTC
CGTTTATTACACTTCTTTATGACACATCCTGAACGTGTTTACAGCCGCGAACAGCTGCTCGATAACGTTTGGGGAACCAA
TGTGTATGTAGAAGACAGAACCGTGGATGTGCACATCCGTCGCCTGCGTAAAGCGGTTGAAGAATCGGGCCATGATCGCC
TCATTCAAACGGTTCGTGGTGCAGGTTATCGTTTCTCTACACGCATTTAG

Upstream 100 bases:

>100_bases
TTATCGCGACAATATCGCTTTTGCGGCATGCTTATTCATAAATCTGTAATAAAAATGACTAATAATAGCCGTGTTGACAT
TCACTGACAGAAAATCACTT

Downstream 100 bases:

>100_bases
TGCCAAATAAGAGATAGGCACAGGTTAGCTTTTAAGCTATCTTGTGCTCCTCCTATTTAGCCTGATGTCTGGGTTTATTT
ATGTTTGACTCTTATTCAGG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPII
MLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRSAPTRLEETIDVQGLLLDPVSHRVSVGDTVLDMGPTEF
RLLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEESGHDRLIQTVRGAGYRFSTRI

Sequences:

>Translated_229_residues
MTARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPII
MLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRSAPTRLEETIDVQGLLLDPVSHRVSVGDTVLDMGPTEF
RLLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEESGHDRLIQTVRGAGYRFSTRI
>Mature_228_residues
TARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPIIM
LTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRSAPTRLEETIDVQGLLLDPVSHRVSVGDTVLDMGPTEFR
LLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEESGHDRLIQTVRGAGYRFSTRI

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=73.7991266375546, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=40.7079646017699, Blast_Score=166, Evalue=8e-43,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.9912663755458, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=38.1165919282511, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=38.1818181818182, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=38.3259911894273, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=41.0480349344978, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=37.0212765957447, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.8744394618834, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=35, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=30.9734513274336, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=30.3418803418803, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=41.2280701754386, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=25, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1790299, Length=152, Percent_Identity=25.6578947368421, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26180; Mature: 26049

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGS
CCEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GIQLAKRLKQDEFTRQIPIIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRSAPTRLEETIDVQGLLLDPVSHRVSVGDTVLDMGPTEFRLLHFFMTHPERVYSREQLL
HCCCCCHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVEESGHDRLIQTVRGAGYRFSTRI
HHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGS
CEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GIQLAKRLKQDEFTRQIPIIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRSAPTRLEETIDVQGLLLDPVSHRVSVGDTVLDMGPTEFRLLHFFMTHPERVYSREQLL
HCCCCCHHHHHHCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVEESGHDRLIQTVRGAGYRFSTRI
HHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]