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The map label for this gene is mak [H]

Identifier: 117921231

GI number: 117921231

Start: 3330001

End: 3330921

Strand: Reverse

Name: mak [H]

Synonym: Shewana3_2790

Alternate gene names: 117921231

Gene position: 3330921-3330001 (Counterclockwise)

Preceding gene: 117921232

Following gene: 117921230

Centisome position: 66.99

GC content: 52.77

Gene sequence:

>921_bases
ATGATACGCATGGGCATCGATTTAGGTGGCACAAAAATAGAACTCGTCGCCTTAAGTGAGGAAGGTAATGAACTGTTTCG
TAAGCGTATCAATACCCCTAGGGATTATCAGGGCACACTGAATGCCATCGTCGATTTAGTGAATGAGGCCGAAGCGACCT
TAGGTGAAAAGGGCACGGTTGGCGTGGGGATCCCGGGAGTCATTTCTCCCTATTCAGGATTAGTCAAAAATGCCAACTCG
ACTTGGATCAACGGTCATCCCCTCGATGTGAATTTAGGGGAGCTGCTCGGCCGTGAAGTGCGAGTCGCTAACGATGCCAA
CTGTTTTGCGGTATCCGAGGCCGTCGATGGTGCTGCTGCGGGTAAATCCGTGGTGTTCGGGGTGATCATTGGCACGGGCT
GCGGCGCGGGTGTGGCCATTAACGGTAAAGTACACGCTGGCGGCAATGGTATTGGCGGTGAGTGGGGCCATAATCCGCTG
CCTTGGATGACCAAGGAGGAGTTTAATACTACCCGTTGTTTCTGCGGGAATCCCGATTGTATCGAAACCTTTATTTCTGG
CACGGGCTTTGTTCGCGATTACAACGAGGCCCTCAGCCGTGCGGTGAATGTCCAAGCTGCACCCGCTAAATCTGGCACTG
AGATCATGGCCTTAGTCGATGCGGGTGATGAAATGGCGATCGCGGCCTTTGATCGTTACATGGACAGACTGGCGCGCTCA
CTCGCCCATGTGATTAATATGCTCGACCCCGATGCGATTGTCCTCGGCGGTGGCATGTCTAATATTGAGGCGACCTATCC
AAGATTACCTGCATTATTAACTCGTTATGTTGTGGGGCGTGAATGCCGTACTCCAGTGGTGCAGAATCTCTATGGCTGCT
CTTCGGGTGTGCGCGGCGCCGCTTGGTTATGGGAAAAATAA

Upstream 100 bases:

>100_bases
CTTATTTTATTGCCACGGCACCAGGCTGGGCAGAATAACGAACAATGCCAAGGGCATCTGTACTGACGCCTTGGTCGAAT
AACAGTGGAGTAAGTCATTC

Downstream 100 bases:

>100_bases
CCCAGTAACGTTAGGTTTTCACTCGTGACAACGCATCGCCTTGGCGATGCGTTTTGCTTTCTTTTGATGTTATGATTGCG
CTCCTTTGAACGATTGGGCT

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIRMGIDLGGTKIELVALSEEGNELFRKRINTPRDYQGTLNAIVDLVNEAEATLGEKGTVGVGIPGVISPYSGLVKNANS
TWINGHPLDVNLGELLGREVRVANDANCFAVSEAVDGAAAGKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNEALSRAVNVQAAPAKSGTEIMALVDAGDEMAIAAFDRYMDRLARS
LAHVINMLDPDAIVLGGGMSNIEATYPRLPALLTRYVVGRECRTPVVQNLYGCSSGVRGAAWLWEK

Sequences:

>Translated_306_residues
MIRMGIDLGGTKIELVALSEEGNELFRKRINTPRDYQGTLNAIVDLVNEAEATLGEKGTVGVGIPGVISPYSGLVKNANS
TWINGHPLDVNLGELLGREVRVANDANCFAVSEAVDGAAAGKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNEALSRAVNVQAAPAKSGTEIMALVDAGDEMAIAAFDRYMDRLARS
LAHVINMLDPDAIVLGGGMSNIEATYPRLPALLTRYVVGRECRTPVVQNLYGCSSGVRGAAWLWEK
>Mature_306_residues
MIRMGIDLGGTKIELVALSEEGNELFRKRINTPRDYQGTLNAIVDLVNEAEATLGEKGTVGVGIPGVISPYSGLVKNANS
TWINGHPLDVNLGELLGREVRVANDANCFAVSEAVDGAAAGKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNEALSRAVNVQAAPAKSGTEIMALVDAGDEMAIAAFDRYMDRLARS
LAHVINMLDPDAIVLGGGMSNIEATYPRLPALLTRYVVGRECRTPVVQNLYGCSSGVRGAAWLWEK

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=309, Percent_Identity=58.252427184466, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787363, Length=305, Percent_Identity=40.9836065573771, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1790522, Length=308, Percent_Identity=26.9480519480519, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786891, Length=276, Percent_Identity=25.3623188405797, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787878, Length=252, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32371; Mature: 32371

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRMGIDLGGTKIELVALSEEGNELFRKRINTPRDYQGTLNAIVDLVNEAEATLGEKGTV
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVISPYSGLVKNANSTWINGHPLDVNLGELLGREVRVANDANCFAVSEAVDGAAA
CCCCCCHHCCHHHHHHCCCCCEECCCCCCCCHHHHCCCEEEECCCCCEEEEHHHHCCHHC
GKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPLPWMTKEEFNTTRCFCGNPDC
CCCEEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYNEALSRAVNVQAAPAKSGTEIMALVDAGDEMAIAAFDRYMDRLARS
HHHHHCCCCCCHHHHHHHHHHHCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LAHVINMLDPDAIVLGGGMSNIEATYPRLPALLTRYVVGRECRTPVVQNLYGCSSGVRGA
HHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
AWLWEK
CCCCCC
>Mature Secondary Structure
MIRMGIDLGGTKIELVALSEEGNELFRKRINTPRDYQGTLNAIVDLVNEAEATLGEKGTV
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVISPYSGLVKNANSTWINGHPLDVNLGELLGREVRVANDANCFAVSEAVDGAAA
CCCCCCHHCCHHHHHHCCCCCEECCCCCCCCHHHHCCCEEEECCCCCEEEEHHHHCCHHC
GKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPLPWMTKEEFNTTRCFCGNPDC
CCCEEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYNEALSRAVNVQAAPAKSGTEIMALVDAGDEMAIAAFDRYMDRLARS
HHHHHCCCCCCHHHHHHHHHHHCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LAHVINMLDPDAIVLGGGMSNIEATYPRLPALLTRYVVGRECRTPVVQNLYGCSSGVRGA
HHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
AWLWEK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]