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The map label for this gene is glnS [H]

Identifier: 117921110

GI number: 117921110

Start: 3185503

End: 3187173

Strand: Reverse

Name: glnS [H]

Synonym: Shewana3_2669

Alternate gene names: 117921110

Gene position: 3187173-3185503 (Counterclockwise)

Preceding gene: 117921111

Following gene: 117921108

Centisome position: 64.1

GC content: 46.56

Gene sequence:

>1671_bases
ATGAGTCATGTGGACACTGAAGTACGTCCGAGTAACTTTATCCGTAATATCATTGATGAGGATCTGAAAAGCGGTAAGCA
CACCAGTGTGCAGACGCGTTTTCCTCCCGAGCCAAATGGTTATCTGCATATTGGTCATGCTAAGTCTATCTGTTTGAACT
TCGGCATCGCGCGCGATTACCAAGGCCTTTGTAATTTACGTTTTGATGATACCAATCCTGAAAAAGAAGACATCGATTAT
GTGAATTCTATTCAGGCGGATGTGCGTTGGTTGGGATTCCAGTGGGATGGTGAGGTTCGTTATTCCTCTAACTATTTCGA
CCAATTACACCAATACGCGGTTGAGTTGATCAATAAAGGCTTAGCCTATGTGTGTTTCCTCAATGCCGATGAAACCCGCG
AATACCGTGGCACCTTAAAAGAGCCGGGTAAAAACAGCCCGTACCGTGACACTTCCGTTGAAGAAAACCTGCAATTGTTT
GAAAAAATGCGTAAGGGTGAATTCAAAGAAGGTGAGTGTGCGCTGCGTGCCAAAATCGACATGGCATCGCCTTTCATGTG
TATGCGCGATCCGGTGATTTACCGTATTCGTTTCGCTCACCATCATCAAACGGGTGACAAGTGGTGCATTTACCCAATGT
ACGACTTCACTCACTGTATTTCTGACGCGCTGGAGCACATTACTCACTCGTTATGTACGCTTGAGTTCCAAGACAACCGT
CGTCTTTACGATTGGGTTCTCGACAATTTAGATGATTTTCAAGCGCCGAACCGTACCCGTCAGTACGAATTTTCTCGCTT
AAATCTTGAATATACGCTGATGTCTAAGCGTAAATTAAATGACTTAGTGACGCGTAAGTTAGTTTCTGGATGGGATGACC
CACGTATGCCAACAATTGCTGGCCTGCGTCGTCGTGGTTATACCCCAGCGTCTATCCGCGAGTTTTGCCAACGTATTGGT
ATCACTAAGCAAGAAAACATGATTGAAGCGGCAATGCTGGATGCTTGTATCCGTGAAGAGCTTAACGAGCATGCGCCACG
TGCGATGGCCGTACTGCGCCCATTAAAAGTCGTGATTGAGAACTACCCAGAAGGTCAAATCGAGACGATTCAAGCGGCTT
CTCACCCAAGCGATGAAAGCATGGGCACACGCGAACTTGCCTTTGGCCGCGAACTGTATATCGATGTGGCGGACTTCAGA
GAAGAAGCCAATAAGCAGTACAAGCGTCTAGTGATGGGCAAAGAAGTGCGCCTGCGTAATGCTTATGTGATTAAGGCTGA
GCGTTGTGACAAAGACAGCGAAGGCAATATCACCACGGTTTACTGTAGCTACGATGCGGATACCTTGGGTAAAAACCCTG
CCGATGGTCGCAAGGTAAAAGGCGTAATCCATTGGGTTGAAGCGACTACGGCCAAACCTGCGGAATTCCGTTTATACCAA
CGCTTGTTCACGGATCCAAATCCTGCAGCAGCAGAGACCGTGGATGAAGTGCTTAACCCTAACTCACTTGAAGTGGTTAA
TGGTTTAGTTGAGGCAAGTCTTGTTAATGCGCCAGCGGAAAAAGCATACCAGTTTGAACGTGAAGGTTATTTCTGTGCTG
ACAATAAAGACTCATCTGCCGAGCATTTAGTCTTTAACCTAACAGTTGCACTGCGTGACTCGTTTGAATAG

Upstream 100 bases:

>100_bases
CATAAACCATAGTTTTTTATACTGGGCATTGTAACTGGCCGAGATCTGTAGGATCATGCGGGTTTTGTTAAAATTGCCAT
TGTTCGCAAAGAGGAATTCC

Downstream 100 bases:

>100_bases
TAAGTCGTTCGCCGCGATAATCGCGGCTGCGCTATGATGCATTACTGTCGCTGCTAGCAACAAACAGCATCAACGAATAA
AAAGGCCCTTAGTGATATAA

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSHVDTEVRPSNFIRNIIDEDLKSGKHTSVQTRFPPEPNGYLHIGHAKSICLNFGIARDYQGLCNLRFDDTNPEKEDIDY
VNSIQADVRWLGFQWDGEVRYSSNYFDQLHQYAVELINKGLAYVCFLNADETREYRGTLKEPGKNSPYRDTSVEENLQLF
EKMRKGEFKEGECALRAKIDMASPFMCMRDPVIYRIRFAHHHQTGDKWCIYPMYDFTHCISDALEHITHSLCTLEFQDNR
RLYDWVLDNLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVTRKLVSGWDDPRMPTIAGLRRRGYTPASIREFCQRIG
ITKQENMIEAAMLDACIREELNEHAPRAMAVLRPLKVVIENYPEGQIETIQAASHPSDESMGTRELAFGRELYIDVADFR
EEANKQYKRLVMGKEVRLRNAYVIKAERCDKDSEGNITTVYCSYDADTLGKNPADGRKVKGVIHWVEATTAKPAEFRLYQ
RLFTDPNPAAAETVDEVLNPNSLEVVNGLVEASLVNAPAEKAYQFEREGYFCADNKDSSAEHLVFNLTVALRDSFE

Sequences:

>Translated_556_residues
MSHVDTEVRPSNFIRNIIDEDLKSGKHTSVQTRFPPEPNGYLHIGHAKSICLNFGIARDYQGLCNLRFDDTNPEKEDIDY
VNSIQADVRWLGFQWDGEVRYSSNYFDQLHQYAVELINKGLAYVCFLNADETREYRGTLKEPGKNSPYRDTSVEENLQLF
EKMRKGEFKEGECALRAKIDMASPFMCMRDPVIYRIRFAHHHQTGDKWCIYPMYDFTHCISDALEHITHSLCTLEFQDNR
RLYDWVLDNLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVTRKLVSGWDDPRMPTIAGLRRRGYTPASIREFCQRIG
ITKQENMIEAAMLDACIREELNEHAPRAMAVLRPLKVVIENYPEGQIETIQAASHPSDESMGTRELAFGRELYIDVADFR
EEANKQYKRLVMGKEVRLRNAYVIKAERCDKDSEGNITTVYCSYDADTLGKNPADGRKVKGVIHWVEATTAKPAEFRLYQ
RLFTDPNPAAAETVDEVLNPNSLEVVNGLVEASLVNAPAEKAYQFEREGYFCADNKDSSAEHLVFNLTVALRDSFE
>Mature_555_residues
SHVDTEVRPSNFIRNIIDEDLKSGKHTSVQTRFPPEPNGYLHIGHAKSICLNFGIARDYQGLCNLRFDDTNPEKEDIDYV
NSIQADVRWLGFQWDGEVRYSSNYFDQLHQYAVELINKGLAYVCFLNADETREYRGTLKEPGKNSPYRDTSVEENLQLFE
KMRKGEFKEGECALRAKIDMASPFMCMRDPVIYRIRFAHHHQTGDKWCIYPMYDFTHCISDALEHITHSLCTLEFQDNRR
LYDWVLDNLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVTRKLVSGWDDPRMPTIAGLRRRGYTPASIREFCQRIGI
TKQENMIEAAMLDACIREELNEHAPRAMAVLRPLKVVIENYPEGQIETIQAASHPSDESMGTRELAFGRELYIDVADFRE
EANKQYKRLVMGKEVRLRNAYVIKAERCDKDSEGNITTVYCSYDADTLGKNPADGRKVKGVIHWVEATTAKPAEFRLYQR
LFTDPNPAAAETVDEVLNPNSLEVVNGLVEASLVNAPAEKAYQFEREGYFCADNKDSSAEHLVFNLTVALRDSFE

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=46.2264150943396, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI62241042, Length=517, Percent_Identity=34.2359767891683, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1786895, Length=551, Percent_Identity=72.9582577132486, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=119, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=536, Percent_Identity=43.8432835820896, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17511007, Length=524, Percent_Identity=32.0610687022901, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324742, Length=566, Percent_Identity=38.5159010600707, Blast_Score=372, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321192, Length=533, Percent_Identity=33.5834896810507, Blast_Score=279, Evalue=9e-76,
Organism=Drosophila melanogaster, GI17864488, Length=534, Percent_Identity=42.5093632958801, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24649466, Length=421, Percent_Identity=39.667458432304, Blast_Score=277, Evalue=2e-74,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 64171; Mature: 64040

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVDTEVRPSNFIRNIIDEDLKSGKHTSVQTRFPPEPNGYLHIGHAKSICLNFGIARDY
CCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHCCCCCCC
QGLCNLRFDDTNPEKEDIDYVNSIQADVRWLGFQWDGEVRYSSNYFDQLHQYAVELINKG
CCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCC
LAYVCFLNADETREYRGTLKEPGKNSPYRDTSVEENLQLFEKMRKGEFKEGECALRAKID
CEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEC
MASPFMCMRDPVIYRIRFAHHHQTGDKWCIYPMYDFTHCISDALEHITHSLCTLEFQDNR
CCCCCHHHCCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
RLYDWVLDNLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVTRKLVSGWDDPRMPTIA
CHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHH
GLRRRGYTPASIREFCQRIGITKQENMIEAAMLDACIREELNEHAPRAMAVLRPLKVVIE
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NYPEGQIETIQAASHPSDESMGTRELAFGRELYIDVADFREEANKQYKRLVMGKEVRLRN
CCCCCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEECC
AYVIKAERCDKDSEGNITTVYCSYDADTLGKNPADGRKVKGVIHWVEATTAKPAEFRLYQ
EEEEEHHHCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHEEEEEECCCCCCHHHHHHH
RLFTDPNPAAAETVDEVLNPNSLEVVNGLVEASLVNAPAEKAYQFEREGYFCADNKDSSA
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCC
EHLVFNLTVALRDSFE
CEEEEEEEEEECCCCC
>Mature Secondary Structure 
SHVDTEVRPSNFIRNIIDEDLKSGKHTSVQTRFPPEPNGYLHIGHAKSICLNFGIARDY
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHCCCCCCC
QGLCNLRFDDTNPEKEDIDYVNSIQADVRWLGFQWDGEVRYSSNYFDQLHQYAVELINKG
CCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCC
LAYVCFLNADETREYRGTLKEPGKNSPYRDTSVEENLQLFEKMRKGEFKEGECALRAKID
CEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEC
MASPFMCMRDPVIYRIRFAHHHQTGDKWCIYPMYDFTHCISDALEHITHSLCTLEFQDNR
CCCCCHHHCCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
RLYDWVLDNLDDFQAPNRTRQYEFSRLNLEYTLMSKRKLNDLVTRKLVSGWDDPRMPTIA
CHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHH
GLRRRGYTPASIREFCQRIGITKQENMIEAAMLDACIREELNEHAPRAMAVLRPLKVVIE
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
NYPEGQIETIQAASHPSDESMGTRELAFGRELYIDVADFREEANKQYKRLVMGKEVRLRN
CCCCCCEEEEECCCCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEECC
AYVIKAERCDKDSEGNITTVYCSYDADTLGKNPADGRKVKGVIHWVEATTAKPAEFRLYQ
EEEEEHHHCCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHEEEEEECCCCCCHHHHHHH
RLFTDPNPAAAETVDEVLNPNSLEVVNGLVEASLVNAPAEKAYQFEREGYFCADNKDSSA
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCCCC
EHLVFNLTVALRDSFE
CEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]