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The map label for this gene is bioF [H]

Identifier: 117920961

GI number: 117920961

Start: 3009588

End: 3010808

Strand: Reverse

Name: bioF [H]

Synonym: Shewana3_2518

Alternate gene names: 117920961

Gene position: 3010808-3009588 (Counterclockwise)

Preceding gene: 117920962

Following gene: 117920960

Centisome position: 60.55

GC content: 52.09

Gene sequence:

>1221_bases
ATGAGTTCACCACTCACCTCAAAACTGGGTTCAAAAATCCTCGCCCGTCAGCAGGCGTTAGCCGAGCAAGGTTTGCTTAG
GCAGCGTCAGGCATTGTCGAGCGCGGTAGCACTCAGTGAGGATGGTCCCCAGTTTGGCTTAGCGGAGCAGCACTATCTTA
ACTTCAGCAGTAATGATTACTTAGGCCTTTCCCGTGCTCCCGCATTGGTCGAGGCGCTGCGCCTTGGCGCTAAGCAATAT
GGCGTTGGTAGCGGCGCATCACCGCTGGTGACGGGCTACAGCGAGGCGCATTTGGCGCTAGAAACTAAGCTGTGCCAGAT
AACAGGATTTGAGGCGGCGCTACTCTTTAGCTCAGGGTTTAGTGCCAATACTACCTTGTGTAAAACCTTGTTCGATAAGC
AGGATGTGGTGCTGGCCGATAAACTGGTTCATGCATCGATTATCGATGGATTACGCGATAGCGGCGCGGATTTTAAGCGA
TTTTTGCATAATTCCACCGAAAGCGCCGAGCGTCTATTAGCGAAGAACGCTGTATCTGCCCTGATCACTGAGAGTGTGTT
CAGCATGGATGGTGATATTGCGCCGATTTCGGCGTTATCCGCACTTTGCCGAGCACATAATGCTTGGCTAATTGTCGATG
ATGCCCATGGCTTTGGCGTTGTTGATGCTGTGAGTGTTCAAGCTGAGTCTACTCCAGCTTCAAACCTTATCGATATCCAG
ATAGTGACCTTTGGTAAGGCGTTAGGCTGTCAGGGAGCCGCGATTCTTGGTTGTCGGCAGTTGATTGAATTTCTGGTCAG
CAACGCACGGGAATATATCTATTCCACCGCCTTGTCGCCCGCCAATGCGGCCCTTGCCTTGGCCGCTGTTGAATACACTG
AGGCCCATCCTGAGCTTAAGCAAAAATTGCAGCGCAATATCTTATTGTTCAAGCAGTTGTGCCAAGATGCTGACATTCCA
CTCCTAGGTAGCGACACGGCGATTCAACCTTTGATTATTGGTGATGCGACGCAAACCATGCGGGTGGCGGAAAAGCTAAA
GGCATTAGGCATTTGGGTCGGCGCTATTCGCCCACCCACAGTGCCGGTCGGCTCGGCAAGATTGCGCATTACCTTAAGCG
CATCCCATAGCGAGGCGGTAATAAGGTGCTGTGTCAGTAGTATTGCCACTGTGCTGAAGGAATGCGCACTGCCCAGGGTT
TCTGAGCGGCCTGAAGGGTAA

Upstream 100 bases:

>100_bases
CGAAAAACCAGTCAAGACGCCGAGTAAGCCAGCAAAGTAAAATACACAGCAAGAAAAGTTGAGCAAGAGAAATTGAGTAA
GAAAAGTTGAGTAAGAAAAA

Downstream 100 bases:

>100_bases
GCCTGCGTTTGATTCAGAATATCCGGTGAATGGCAGGGCACTTCAATGCTCGGTATTGAGTTCTTGAGAGTGAAGAGCTT
GAAGATAAGAGCTTGAATGT

Product: aminotransferase, class I and II

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSSPLTSKLGSKILARQQALAEQGLLRQRQALSSAVALSEDGPQFGLAEQHYLNFSSNDYLGLSRAPALVEALRLGAKQY
GVGSGASPLVTGYSEAHLALETKLCQITGFEAALLFSSGFSANTTLCKTLFDKQDVVLADKLVHASIIDGLRDSGADFKR
FLHNSTESAERLLAKNAVSALITESVFSMDGDIAPISALSALCRAHNAWLIVDDAHGFGVVDAVSVQAESTPASNLIDIQ
IVTFGKALGCQGAAILGCRQLIEFLVSNAREYIYSTALSPANAALALAAVEYTEAHPELKQKLQRNILLFKQLCQDADIP
LLGSDTAIQPLIIGDATQTMRVAEKLKALGIWVGAIRPPTVPVGSARLRITLSASHSEAVIRCCVSSIATVLKECALPRV
SERPEG

Sequences:

>Translated_406_residues
MSSPLTSKLGSKILARQQALAEQGLLRQRQALSSAVALSEDGPQFGLAEQHYLNFSSNDYLGLSRAPALVEALRLGAKQY
GVGSGASPLVTGYSEAHLALETKLCQITGFEAALLFSSGFSANTTLCKTLFDKQDVVLADKLVHASIIDGLRDSGADFKR
FLHNSTESAERLLAKNAVSALITESVFSMDGDIAPISALSALCRAHNAWLIVDDAHGFGVVDAVSVQAESTPASNLIDIQ
IVTFGKALGCQGAAILGCRQLIEFLVSNAREYIYSTALSPANAALALAAVEYTEAHPELKQKLQRNILLFKQLCQDADIP
LLGSDTAIQPLIIGDATQTMRVAEKLKALGIWVGAIRPPTVPVGSARLRITLSASHSEAVIRCCVSSIATVLKECALPRV
SERPEG
>Mature_405_residues
SSPLTSKLGSKILARQQALAEQGLLRQRQALSSAVALSEDGPQFGLAEQHYLNFSSNDYLGLSRAPALVEALRLGAKQYG
VGSGASPLVTGYSEAHLALETKLCQITGFEAALLFSSGFSANTTLCKTLFDKQDVVLADKLVHASIIDGLRDSGADFKRF
LHNSTESAERLLAKNAVSALITESVFSMDGDIAPISALSALCRAHNAWLIVDDAHGFGVVDAVSVQAESTPASNLIDIQI
VTFGKALGCQGAAILGCRQLIEFLVSNAREYIYSTALSPANAALALAAVEYTEAHPELKQKLQRNILLFKQLCQDADIPL
LGSDTAIQPLIIGDATQTMRVAEKLKALGIWVGAIRPPTVPVGSARLRITLSASHSEAVIRCCVSSIATVLKECALPRVS
ERPEG

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI83977442, Length=356, Percent_Identity=32.5842696629214, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI83977444, Length=356, Percent_Identity=32.5842696629214, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI83977440, Length=356, Percent_Identity=32.5842696629214, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI7657118, Length=374, Percent_Identity=31.8181818181818, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI284448556, Length=352, Percent_Identity=32.3863636363636, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4502025, Length=352, Percent_Identity=32.3863636363636, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI40316939, Length=352, Percent_Identity=32.3863636363636, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4758668, Length=367, Percent_Identity=26.7029972752044, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI119220554, Length=358, Percent_Identity=25.6983240223464, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI5454084, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786993, Length=338, Percent_Identity=43.491124260355, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790046, Length=354, Percent_Identity=34.7457627118644, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=353, Percent_Identity=30.8781869688385, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI32566772, Length=352, Percent_Identity=26.9886363636364, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=349, Percent_Identity=26.647564469914, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=355, Percent_Identity=27.0422535211268, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982625, Length=296, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982617, Length=296, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320438, Length=377, Percent_Identity=30.5039787798408, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=367, Percent_Identity=24.5231607629428, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323954, Length=222, Percent_Identity=27.4774774774775, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24662918, Length=348, Percent_Identity=35.3448275862069, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI17137420, Length=351, Percent_Identity=30.7692307692308, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17136286, Length=390, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24653280, Length=312, Percent_Identity=24.6794871794872, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653276, Length=312, Percent_Identity=24.6794871794872, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653278, Length=312, Percent_Identity=24.6794871794872, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 43083; Mature: 42952

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPLTSKLGSKILARQQALAEQGLLRQRQALSSAVALSEDGPQFGLAEQHYLNFSSNDY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCC
LGLSRAPALVEALRLGAKQYGVGSGASPLVTGYSEAHLALETKLCQITGFEAALLFSSGF
CCCCHHHHHHHHHHHCHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
SANTTLCKTLFDKQDVVLADKLVHASIIDGLRDSGADFKRFLHNSTESAERLLAKNAVSA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHH
LITESVFSMDGDIAPISALSALCRAHNAWLIVDDAHGFGVVDAVSVQAESTPASNLIDIQ
HHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCCCEEEEE
IVTFGKALGCQGAAILGCRQLIEFLVSNAREYIYSTALSPANAALALAAVEYTEAHPELK
EEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
QKLQRNILLFKQLCQDADIPLLGSDTAIQPLIIGDATQTMRVAEKLKALGIWVGAIRPPT
HHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCCC
VPVGSARLRITLSASHSEAVIRCCVSSIATVLKECALPRVSERPEG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSPLTSKLGSKILARQQALAEQGLLRQRQALSSAVALSEDGPQFGLAEQHYLNFSSNDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCC
LGLSRAPALVEALRLGAKQYGVGSGASPLVTGYSEAHLALETKLCQITGFEAALLFSSGF
CCCCHHHHHHHHHHHCHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
SANTTLCKTLFDKQDVVLADKLVHASIIDGLRDSGADFKRFLHNSTESAERLLAKNAVSA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHH
LITESVFSMDGDIAPISALSALCRAHNAWLIVDDAHGFGVVDAVSVQAESTPASNLIDIQ
HHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEECCCCCCCEEEEE
IVTFGKALGCQGAAILGCRQLIEFLVSNAREYIYSTALSPANAALALAAVEYTEAHPELK
EEEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
QKLQRNILLFKQLCQDADIPLLGSDTAIQPLIIGDATQTMRVAEKLKALGIWVGAIRPPT
HHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHEECCCCCC
VPVGSARLRITLSASHSEAVIRCCVSSIATVLKECALPRVSERPEG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]