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The map label for this gene is bioB [H]

Identifier: 117920962

GI number: 117920962

Start: 3011043

End: 3012095

Strand: Reverse

Name: bioB [H]

Synonym: Shewana3_2519

Alternate gene names: 117920962

Gene position: 3012095-3011043 (Counterclockwise)

Preceding gene: 117920965

Following gene: 117920961

Centisome position: 60.58

GC content: 50.81

Gene sequence:

>1053_bases
ATGTCGCAGTTGCAAGTTCGTCATGATTGGAAGCGGGAAGAAATCGAAGCCTTATTTGCGCTGCCGATGAATGACTTATT
ATTTAAAGCACACAGTATCCACCGTGAAGTGTACGATCCTAACGAAGTGCAGATCAGCCGATTGCTGTCGATCAAAACTG
GTGCTTGTCCTGAGGATTGTAAATATTGTCCGCAGAGTGCGCGTTACGACACTGGCCTTGAAAAAGAGCGCCTATTGGCG
ATGGAAACCGTATTGACCGAAGCGCGCAGTGCCAAAGCCGCGGGCGCTTCACGTTTCTGTATGGGCGCCGCATGGCGTAA
CCCGAAAGATAAAGACATGCCATACCTCAAGCAAATGGTGCAAGAGGTCAAAGCCCTCGGCATGGAAACCTGTATGACCC
TTGGCATGCTAAGTGCTGAACAAGCCAACGAGCTGGCCGATGCAGGTCTTGATTACTACAACCACAATTTAGATACCTCG
CCTGAATATTACGGCGATGTGATCACGACCCGTACCTATCAAAACCGTTTAGATACCTTAAGCCACGTGCGTGCATCGGG
CATGAAAGTTTGCTCTGGCGGTATTGTTGGTATGGGCGAAAAGGCCACAGACCGAGCGGGCTTATTGCAACAACTGGCTA
ATTTGCCTCAGCATCCCGATTCTGTGCCCATTAACATGTTGGTCAAAGTGGCGGGTACCCCCTTCGAAAAACTCGATGAT
TTAGATCCGCTCGAGTTTGTCCGTACTATCGCCGTGGCGCGTATTTTAATGCCTAAGTCCCGTGTGCGTTTATCGGCTGG
CCGTGAAAACATGACTGACGAACTGCAGGCCATGTGTTTCTTTGCTGGCGCGAACTCGATTTTCTATGGCTGCAAGCTGT
TGACTACGCCAAACCCTGAAGAAAGTGATGATATGGGCCTGTTCCGCCGTCTGGGATTACGTCCTGAGCAGGGTGCTGCC
GCGACTATCGATGATGAACAGGCGGTATTAGCCAAAGCTGCTGCCCACCAAGATAAAGCCTCGGCACCTTTTTATGATGC
GGCGGCGCTGTAA

Upstream 100 bases:

>100_bases
AAATTAATCAGTGGTAAACCTGTATCACTGGTTGGCGTTGACAGTTTAAGGGCTGAGGTTATCCTAGCCAAGGTAAAATA
CAAAAATAAAAGGGTGTTCA

Downstream 100 bases:

>100_bases
CTAAGGCTTATCGCCACAGTAAAACTTGCTACGTTGGACTTGCGTTTTGTTAAGTGGCGAAGCGCAGGTTCCAAAGGATG
ATGCGGGCGGTGAGACTCAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKDKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLSHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPKSRVRLSAGRENMTDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAA
ATIDDEQAVLAKAAAHQDKASAPFYDAAAL

Sequences:

>Translated_350_residues
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKDKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTS
PEYYGDVITTRTYQNRLDTLSHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
LDPLEFVRTIAVARILMPKSRVRLSAGRENMTDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAA
ATIDDEQAVLAKAAAHQDKASAPFYDAAAL
>Mature_349_residues
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLAM
ETVLTEARSAKAAGASRFCMGAAWRNPKDKDMPYLKQMVQEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSP
EYYGDVITTRTYQNRLDTLSHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDDL
DPLEFVRTIAVARILMPKSRVRLSAGRENMTDELQAMCFFAGANSIFYGCKLLTTPNPEESDDMGLFRRLGLRPEQGAAA
TIDDEQAVLAKAAAHQDKASAPFYDAAAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=345, Percent_Identity=66.3768115942029, Blast_Score=481, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=46.8354430379747, Blast_Score=313, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38733; Mature: 38601

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKDKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
SHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPKSRVRLSAGRENMTDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAAATIDDEQAVLAKAAAHQDKASAPFYDAAAL
CCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure 
SQLQVRHDWKREEIEALFALPMNDLLFKAHSIHREVYDPNEVQISRLLSIKTGACPEDC
CCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKDKDMPYLKQMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
QEVKALGMETCMTLGMLSAEQANELADAGLDYYNHNLDTSPEYYGDVITTRTYQNRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
SHVRASGMKVCSGGIVGMGEKATDRAGLLQQLANLPQHPDSVPINMLVKVAGTPFEKLDD
HHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHCC
LDPLEFVRTIAVARILMPKSRVRLSAGRENMTDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ESDDMGLFRRLGLRPEQGAAATIDDEQAVLAKAAAHQDKASAPFYDAAAL
CCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA