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The map label for this gene is yafJ [H]

Identifier: 117920765

GI number: 117920765

Start: 2794401

End: 2795240

Strand: Reverse

Name: yafJ [H]

Synonym: Shewana3_2322

Alternate gene names: 117920765

Gene position: 2795240-2794401 (Counterclockwise)

Preceding gene: 117920767

Following gene: 117920762

Centisome position: 56.22

GC content: 48.57

Gene sequence:

>840_bases
ATGTCATCAACTAGTTACAATGCAAAACCGTGGATTTTTGGATTAAGAAAGGCGAGAAATATGTGTGAGTTACTGGCGAT
GAGCGCCAATGTGCCGACCGATATTGTGTTTAGTTTTACTGGGTTAGCACAGCGTGGTGGCGTCACAGGGCCCCATGTCG
ACGGTTGGGGGATCACTTTCTATGAGGGCCGTGGCAGCCGTACTTTTAAGGATGCCTGCCCGAGTAGTGAGTCGCACATT
GCAAAGTTGATCAAATCTTATCCGATTAAAAGCGAGGTGGTGATAAGCCATATTCGCCAAGCTAATCGTGGCTGTGTCTC
CCTTGAAAATACCCACCCATTTACCCGTGAGTTGTGGGGCCGTTATTGGACCTATGCCCACAATGGCCAGTTGAGCGACT
ATCAGGCGAAGTTTCAGGTTTCCCGTTATCAGAACGTTGGCGATACCGACAGTGAACTGGCTTTTTGCTGGATACTGGAG
CAAATTGCTGCCAAGTTTGGTGACCGTCGCCCAGAGGACATGCAAGCGGTGTTTCGGTTTGTGGCTACGCTTGCCGAACA
AATCCGCGCCCTTGGGGTGTTTAATATGATCCTCAGTGATGGCGAATATTTGATGAGCTATTGCAGTAACAATCTTTGCT
ACATCACCCGCAGGGCACCGTTTGGCAAGGCAAAATTAATTGATACCGATGTTGTGATTGATTTTGATAAAGAAACCACA
CCACACGATGTCGTGACTGTGATTGCGACTCGGCCGCTCACCGAAAACGAAGACTGGCAGGTGATGCAGCCTGGCAATTG
GAAGTTATTCCGCTTAGGTGAGCTGCTTATCGATTTGTAA

Upstream 100 bases:

>100_bases
TCTGACCTGTGTCGCATAAAAGTTAAACCTAAGGCAGTTTTAATACAAGTATTTTTCAAACAATTGTTTAAATTTTTTGT
CACCTTTGATCATTCCACAA

Downstream 100 bases:

>100_bases
TCGGGCGCTGTCACTGTCGTTCTATTTGATGGCATTAAATCAGAGACAAAAGGCCAAACGGAGTCACTCACGTTTGGCCT
TTTGTGTTTTTATGCTGTGC

Product: glutamine amidotransferase, class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSSTSYNAKPWIFGLRKARNMCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGRGSRTFKDACPSSESHI
AKLIKSYPIKSEVVISHIRQANRGCVSLENTHPFTRELWGRYWTYAHNGQLSDYQAKFQVSRYQNVGDTDSELAFCWILE
QIAAKFGDRRPEDMQAVFRFVATLAEQIRALGVFNMILSDGEYLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETT
PHDVVTVIATRPLTENEDWQVMQPGNWKLFRLGELLIDL

Sequences:

>Translated_279_residues
MSSTSYNAKPWIFGLRKARNMCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGRGSRTFKDACPSSESHI
AKLIKSYPIKSEVVISHIRQANRGCVSLENTHPFTRELWGRYWTYAHNGQLSDYQAKFQVSRYQNVGDTDSELAFCWILE
QIAAKFGDRRPEDMQAVFRFVATLAEQIRALGVFNMILSDGEYLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETT
PHDVVTVIATRPLTENEDWQVMQPGNWKLFRLGELLIDL
>Mature_278_residues
SSTSYNAKPWIFGLRKARNMCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITFYEGRGSRTFKDACPSSESHIA
KLIKSYPIKSEVVISHIRQANRGCVSLENTHPFTRELWGRYWTYAHNGQLSDYQAKFQVSRYQNVGDTDSELAFCWILEQ
IAAKFGDRRPEDMQAVFRFVATLAEQIRALGVFNMILSDGEYLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETTP
HDVVTVIATRPLTENEDWQVMQPGNWKLFRLGELLIDL

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=257, Percent_Identity=65.7587548638132, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 31673; Mature: 31542

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTSYNAKPWIFGLRKARNMCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YEGRGSRTFKDACPSSESHIAKLIKSYPIKSEVVISHIRQANRGCVSLENTHPFTRELWG
EECCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
RYWTYAHNGQLSDYQAKFQVSRYQNVGDTDSELAFCWILEQIAAKFGDRRPEDMQAVFRF
HHEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VATLAEQIRALGVFNMILSDGEYLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCC
PHDVVTVIATRPLTENEDWQVMQPGNWKLFRLGELLIDL
CHHEEEEEEECCCCCCCCCEEECCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
SSTSYNAKPWIFGLRKARNMCELLAMSANVPTDIVFSFTGLAQRGGVTGPHVDGWGITF
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YEGRGSRTFKDACPSSESHIAKLIKSYPIKSEVVISHIRQANRGCVSLENTHPFTRELWG
EECCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
RYWTYAHNGQLSDYQAKFQVSRYQNVGDTDSELAFCWILEQIAAKFGDRRPEDMQAVFRF
HHEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VATLAEQIRALGVFNMILSDGEYLMSYCSNNLCYITRRAPFGKAKLIDTDVVIDFDKETT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCEEEECEEEEECCCCCC
PHDVVTVIATRPLTENEDWQVMQPGNWKLFRLGELLIDL
CHHEEEEEEECCCCCCCCCEEECCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]