Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 117920762

GI number: 117920762

Start: 2792153

End: 2792644

Strand: Reverse

Name: def [H]

Synonym: Shewana3_2319

Alternate gene names: 117920762

Gene position: 2792644-2792153 (Counterclockwise)

Preceding gene: 117920765

Following gene: 117920761

Centisome position: 56.17

GC content: 44.11

Gene sequence:

>492_bases
ATGGCTGTACTCGATATTCTGACAATTCCCGATGAGCGTCTAAAGCGCAAAGCACAACCCGTAAAAGACATTGAAGCGAT
TCAGGGATTTATCGATGATTTAATCGAAACCATGTACCACACCGACGACGGTATCGGTCTGGCCTCGACCCAAGTGGGCA
GTACAGATGCGGTGATTGTGATCGATTTATCCGAAACCCGCGATCAACCCTTAGTGCTGATTAATCCAGAGATTGTTGAA
AAATCAGGCGAATACGTAGGTGAAGAAGGCTGTCTTTCTATCCCAGGTTACCGCGCAAAAGTCACTCGTTTTGAAAAGGT
CAAAGTCACCGCCTTAGACAGACAGGGTAAAGCGATTGAAATCGAAACCGATGACTTTTTAGCCATTGTTTTACAACACG
AAATCGACCATTTACACGGTAAAGTGTTTATCGAACACTTATCGGCATTAAAGCAGCAAATCGCGTTGAAAAAAGTGCGT
AAATACGCCTAA

Upstream 100 bases:

>100_bases
AATGATTTTTATTAAACTATTTAGTTTTGAATATTGAGAAAACACAGGAATAGTTCATAATAGCGCCAAATTTTTTGACT
CACTAAGAGATGAAACAGGC

Downstream 100 bases:

>100_bases
GCAGATGAATTTGCGCAACTGATTATTTTAATCATCTAAGCCAAAGCCGCATTCCGATGCGGCTTTGTTGTTTCTAAGCA
TTTAAAAAGCCAAACAAAAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 3; Polypeptide deformylase 3 [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAVLDILTIPDERLKRKAQPVKDIEAIQGFIDDLIETMYHTDDGIGLASTQVGSTDAVIVIDLSETRDQPLVLINPEIVE
KSGEYVGEEGCLSIPGYRAKVTRFEKVKVTALDRQGKAIEIETDDFLAIVLQHEIDHLHGKVFIEHLSALKQQIALKKVR
KYA

Sequences:

>Translated_163_residues
MAVLDILTIPDERLKRKAQPVKDIEAIQGFIDDLIETMYHTDDGIGLASTQVGSTDAVIVIDLSETRDQPLVLINPEIVE
KSGEYVGEEGCLSIPGYRAKVTRFEKVKVTALDRQGKAIEIETDDFLAIVLQHEIDHLHGKVFIEHLSALKQQIALKKVR
KYA
>Mature_162_residues
AVLDILTIPDERLKRKAQPVKDIEAIQGFIDDLIETMYHTDDGIGLASTQVGSTDAVIVIDLSETRDQPLVLINPEIVEK
SGEYVGEEGCLSIPGYRAKVTRFEKVKVTALDRQGKAIEIETDDFLAIVLQHEIDHLHGKVFIEHLSALKQQIALKKVRK
YA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=59.2592592592593, Blast_Score=182, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24645728, Length=160, Percent_Identity=31.25, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645726, Length=122, Percent_Identity=32.7868852459016, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18205; Mature: 18074

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLDILTIPDERLKRKAQPVKDIEAIQGFIDDLIETMYHTDDGIGLASTQVGSTDAVIV
CCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEE
IDLSETRDQPLVLINPEIVEKSGEYVGEEGCLSIPGYRAKVTRFEKVKVTALDRQGKAIE
EECCCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCCHHHEEEEEEEEEEECCCCCEEE
IETDDFLAIVLQHEIDHLHGKVFIEHLSALKQQIALKKVRKYA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVLDILTIPDERLKRKAQPVKDIEAIQGFIDDLIETMYHTDDGIGLASTQVGSTDAVIV
CEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEE
IDLSETRDQPLVLINPEIVEKSGEYVGEEGCLSIPGYRAKVTRFEKVKVTALDRQGKAIE
EECCCCCCCCEEEECHHHHHCCCCCCCCCCCEECCCCCHHHEEEEEEEEEEECCCCCEEE
IETDDFLAIVLQHEIDHLHGKVFIEHLSALKQQIALKKVRKYA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]