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The map label for this gene is bcr [H]

Identifier: 117920711

GI number: 117920711

Start: 2731359

End: 2732582

Strand: Direct

Name: bcr [H]

Synonym: Shewana3_2268

Alternate gene names: 117920711

Gene position: 2731359-2732582 (Clockwise)

Preceding gene: 117920709

Following gene: 117920712

Centisome position: 54.93

GC content: 49.67

Gene sequence:

>1224_bases
GTGGACCCACAACCGTCAAGCTATCCGACTAAACCGAGCGACATTCCCCATTTGGCCCTGCTGATCCCTATGCTCGCCGC
TATTGTTGCGATCACGCCGCTCGCGATCGACATGTATTTGCCCGCCATGGCAACCCTCGCCCATAGTTTCCATACAGATA
TCACCCTAGTGCAACAATCCTTAAGTGTTTACTTGGGCGGCTACGCCTTGGGTATGCTGTGCTTTGGCCCACTTGCAGAC
CGTATTGGTCGTCGCCCCATGGTAATAATGGGTCTCTCGGGCTTTATGGTAGTCAGCCTGTTATTGGGCTTAGCCGAGCA
GATTGAAGTGTTTTTAGGACTGCGCTTTTTACAAGCCTTTATCGGCGCTGCGGCCACTGTGGTCGTCCCTGGGTATATCA
AAGAAGTCTATGGTGAAAACACGGCTAAGGGTATGTCTTATGTCAGTTTAATCATGATGTTAGCACCATTATTAGCACCC
AGCATCGGTAGCCTTATCCTCGAGCTTGGCAAATGGCATTTGATCTTCTTTATCCAATCGGCCTATGCCATGTTGTTGCT
GTTGTTGGTCATTACGTTGCTCAGAATGCCGAGCGATAAAGATCAGAGTAGCCGCAGTCAAAAATCCTTTTTAGGGGCTT
ATGCTACTGTGTTTTCACGTCCCGGCGTCAAAATGAACATTGCCAGTGGCGTGCTTACCTCTTTTGCGTTTTTTTGTTAT
TTAACCGCGTCGCCCTTTGTCTATATGGAGGTTTTTTCCCTAGATAAATCCTTATTCGCCCTGCTCTTTAGTACCAATGT
GGGCGCACTGATGCTGGCAAATATTATCAATACCCGAATTGTGGGCCGTTATGGTTCGTTGAGGATGCTGCACGTGTCGA
CCTTTTTCGGTGCGCTGGCGGCGGTAGGTTTACTGGGAGTGAACTTACTGGGGCTCAGTTATCATTTCACCGTATTGATG
TTGATCCCGCTGATGGGGAGCCTTGGTATCATGTCGGTCAACGCCGATGCTATTGTGCTGATGAAATTCAAGCAGGAAAC
GGGCACTGCAACGGCAGTGATTGGCACCCTAAGATTCGGTTTTGGCGCCCTTGCTGGGCCATTACTCGCGCTGTTTTACA
ATGGCACCGCAGTGCCCTTCTCGGCATTAATGCTGACGGCGGTATTGTTGGTGGGATTCTGTCAATTTAACCGACCGACT
CACCAAGCCAAGCCGAAGGAGTAA

Upstream 100 bases:

>100_bases
GGCGAAATGAGCCGCGAATAGGCAAAATTGTTACCCTACAATTTTGTTTTTCGAGTGACATAACACTTTATATCGCCTTC
TTTCGCCACCCGAGGTAACT

Downstream 100 bases:

>100_bases
TCTGCATCTTTATGCCACGTAAACAATGCAATTGTGGTATTCATCGCTTTTGTGGTTGATTTGTAACAAATTAAGTGTTT
TCTAAATAAACGGCACTCAG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDPQPSSYPTKPSDIPHLALLIPMLAAIVAITPLAIDMYLPAMATLAHSFHTDITLVQQSLSVYLGGYALGMLCFGPLAD
RIGRRPMVIMGLSGFMVVSLLLGLAEQIEVFLGLRFLQAFIGAAATVVVPGYIKEVYGENTAKGMSYVSLIMMLAPLLAP
SIGSLILELGKWHLIFFIQSAYAMLLLLLVITLLRMPSDKDQSSRSQKSFLGAYATVFSRPGVKMNIASGVLTSFAFFCY
LTASPFVYMEVFSLDKSLFALLFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGALAAVGLLGVNLLGLSYHFTVLM
LIPLMGSLGIMSVNADAIVLMKFKQETGTATAVIGTLRFGFGALAGPLLALFYNGTAVPFSALMLTAVLLVGFCQFNRPT
HQAKPKE

Sequences:

>Translated_407_residues
MDPQPSSYPTKPSDIPHLALLIPMLAAIVAITPLAIDMYLPAMATLAHSFHTDITLVQQSLSVYLGGYALGMLCFGPLAD
RIGRRPMVIMGLSGFMVVSLLLGLAEQIEVFLGLRFLQAFIGAAATVVVPGYIKEVYGENTAKGMSYVSLIMMLAPLLAP
SIGSLILELGKWHLIFFIQSAYAMLLLLLVITLLRMPSDKDQSSRSQKSFLGAYATVFSRPGVKMNIASGVLTSFAFFCY
LTASPFVYMEVFSLDKSLFALLFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGALAAVGLLGVNLLGLSYHFTVLM
LIPLMGSLGIMSVNADAIVLMKFKQETGTATAVIGTLRFGFGALAGPLLALFYNGTAVPFSALMLTAVLLVGFCQFNRPT
HQAKPKE
>Mature_407_residues
MDPQPSSYPTKPSDIPHLALLIPMLAAIVAITPLAIDMYLPAMATLAHSFHTDITLVQQSLSVYLGGYALGMLCFGPLAD
RIGRRPMVIMGLSGFMVVSLLLGLAEQIEVFLGLRFLQAFIGAAATVVVPGYIKEVYGENTAKGMSYVSLIMMLAPLLAP
SIGSLILELGKWHLIFFIQSAYAMLLLLLVITLLRMPSDKDQSSRSQKSFLGAYATVFSRPGVKMNIASGVLTSFAFFCY
LTASPFVYMEVFSLDKSLFALLFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGALAAVGLLGVNLLGLSYHFTVLM
LIPLMGSLGIMSVNADAIVLMKFKQETGTATAVIGTLRFGFGALAGPLLALFYNGTAVPFSALMLTAVLLVGFCQFNRPT
HQAKPKE

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=377, Percent_Identity=33.42175066313, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI48994889, Length=371, Percent_Identity=27.4932614555256, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790146, Length=251, Percent_Identity=28.2868525896414, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790794, Length=256, Percent_Identity=29.296875, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787065, Length=270, Percent_Identity=27.4074074074074, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082312, Length=193, Percent_Identity=25.3886010362694, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=195, Percent_Identity=25.1282051282051, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=173, Percent_Identity=27.7456647398844, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43809; Mature: 43809

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPQPSSYPTKPSDIPHLALLIPMLAAIVAITPLAIDMYLPAMATLAHSFHTDITLVQQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVYLGGYALGMLCFGPLADRIGRRPMVIMGLSGFMVVSLLLGLAEQIEVFLGLRFLQAF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAAATVVVPGYIKEVYGENTAKGMSYVSLIMMLAPLLAPSIGSLILELGKWHLIFFIQS
HCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AYAMLLLLLVITLLRMPSDKDQSSRSQKSFLGAYATVFSRPGVKMNIASGVLTSFAFFCY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
LTASPFVYMEVFSLDKSLFALLFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGALA
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
AVGLLGVNLLGLSYHFTVLMLIPLMGSLGIMSVNADAIVLMKFKQETGTATAVIGTLRFG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHH
FGALAGPLLALFYNGTAVPFSALMLTAVLLVGFCQFNRPTHQAKPKE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDPQPSSYPTKPSDIPHLALLIPMLAAIVAITPLAIDMYLPAMATLAHSFHTDITLVQQS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVYLGGYALGMLCFGPLADRIGRRPMVIMGLSGFMVVSLLLGLAEQIEVFLGLRFLQAF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAAATVVVPGYIKEVYGENTAKGMSYVSLIMMLAPLLAPSIGSLILELGKWHLIFFIQS
HCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
AYAMLLLLLVITLLRMPSDKDQSSRSQKSFLGAYATVFSRPGVKMNIASGVLTSFAFFCY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
LTASPFVYMEVFSLDKSLFALLFSTNVGALMLANIINTRIVGRYGSLRMLHVSTFFGALA
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
AVGLLGVNLLGLSYHFTVLMLIPLMGSLGIMSVNADAIVLMKFKQETGTATAVIGTLRFG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEECCCCCHHHHHHHHHHH
FGALAGPLLALFYNGTAVPFSALMLTAVLLVGFCQFNRPTHQAKPKE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]