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The map label for this gene is ilvI [H]

Identifier: 117920712

GI number: 117920712

Start: 2733051

End: 2734769

Strand: Direct

Name: ilvI [H]

Synonym: Shewana3_2269

Alternate gene names: 117920712

Gene position: 2733051-2734769 (Clockwise)

Preceding gene: 117920711

Following gene: 117920713

Centisome position: 54.97

GC content: 49.21

Gene sequence:

>1719_bases
ATGGAGAAGTTATCCGGCGCCAGCATGATTGTGCGATCCCTTATCGATGAAGGTGTAAAGCACATATTTGGTTATCCTGG
CGGCTCAGTGTTAGATATCTACGACGCCCTGCACCTTATCCCCGGTATTGAACATATTCTGGTTCGCCACGAACAAGCCG
CCGTACATATGGCCGATGGTTATGCCCGCGCCACGGGTAAAGTAGGCGTGGTACTCGTGACCTCAGGCCCAGGTGCCACC
AATGCCATTACCGGCATCGCTACCGCCTATATGGATTCAATTCCATTAGTGGTGTTATCCGGCCAAGTACCCAGCAATCT
TATCGGTAATGATGCGTTCCAAGAATGCGACATGATTGGGATTTCACGCCCAGTCGTCAAACACAGCTTTTTAGTACAAG
ATCCTACTCAAATACCTGAAATCATTAAAAAAGCCTTTTATATTGCTTCCACGGGGCGCCCCGGCCCCGTCGTTGTCGAC
TTGCCAAAGGATTGCCTCAATCCTGCATTGCTGCACGACTATATTTACCCCGAGAGCATTAAAATGCGTTCCTATAATCC
AACGACATCGGGCCACAAGGGGCAAATTCGCCGTGGATTACAGGCATTACTGGCGGCGAAAAAACCCGTGCTTTATGTGG
GTGGCGGCGCCATTATCTCCAGCTGTGAAAAGCAAATCTTAGCCCTAGCGGAAAAATTAAATATCCCCGTTGTCAGCACC
TTGATGGGCCTTGGCGCTTTCCCTGGCACCCATAAAAACAGCTTAGGCATGCTGGGGATGCACGGTCGCTATGAAGCCAA
CATGACCATGCATAACTGCGATCTTATCTTCGGGATTGGCGTGCGTTTCGATGACAGAACCACCAACAACATCGAGAAAT
ACTGCCCAAATGCCACTATTTTGCATATCGATATCGACCCTTCATCGATTTCAAAAACCGTCCGAGTGGATATCCCCATC
GTTGGCTCGGCAGACATCGTACTGGACAGCATGTTAGCCCTGCTGGAGGAGTCGAAGGAGAGCAACGATAGCGAGGCAAT
TAATCAATGGTGGCAAGAAATCACAGTATGGCGTAACCGCAATTGCTTGGCATACGATAAGGAAAGCAACCGGATCAAAC
CACAGCAAGTGATTGAAACTCTTTATAAACTCACTAATGGCGAGGCCTATGTTGCCTCGGATGTGGGCCAGCATCAAATG
TTTGCCGCGCTCTATTATCCCTTTGATAAACCTCGCCATTGGATTAACTCCGGCGGCCTTGGCACCATGGGCTTTGGTTT
ACCCGCTGCCATGGGAGTCAAAATGGCAATGCCTGACGAAACCGTTGTGTGCGTCACGGGCGATGGTTCGATTCAAATGA
ATATTCAAGAGCTTTCAACCGCTCTGCAATACGATACGCCGGTTAAAATTATCAACTTAAACAACCGTTTCCTCGGCATG
GTGAAACAGTGGCAGGATATGATCTACTCGGGTCGCCATTCCCATTCCTATATGGATTCGGTACCCAACTTTGCCAAAAT
TGCCGAAGCCTATGGCCATGTTGGCATGACGATTAGCGATCCCGCTGAGCTTGAGTCTAAGATGGCCGAAGCCCTCGCCA
TGAAAGACAGACTGGTATTTATCGACATTATGGTGGATGAGACTGAGCACGTGTATCCCATGCTTATCCGCGGCGGCGCG
ATGAATGAAATGTGGTTAAGCAAAACGGAGAAATGCTAA

Upstream 100 bases:

>100_bases
TCGGAGTCTGAATTACCCTTCAGGCTTATTTTTAGAGCGATTACCGTCGCTGTAACAAGATACTGTTTACCGTAACGGTA
CTATGCAATAGGAGTTTCAC

Downstream 100 bases:

>100_bases
TGCGTCGAATTATATCTGTATTACTCGAAAACCAATCCGGCGCATTATCTCGTGTTGTCGGTCTGTTTTCCCAACGCGGT
TACAACATCGAAAGTTTAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHLIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVQDPTQIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHDYIYPESIKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISSCEKQILALAEKLNIPVVST
LMGLGAFPGTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNIEKYCPNATILHIDIDPSSISKTVRVDIPI
VGSADIVLDSMLALLEESKESNDSEAINQWWQEITVWRNRNCLAYDKESNRIKPQQVIETLYKLTNGEAYVASDVGQHQM
FAALYYPFDKPRHWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLSKTEKC

Sequences:

>Translated_572_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHLIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVQDPTQIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHDYIYPESIKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISSCEKQILALAEKLNIPVVST
LMGLGAFPGTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNIEKYCPNATILHIDIDPSSISKTVRVDIPI
VGSADIVLDSMLALLEESKESNDSEAINQWWQEITVWRNRNCLAYDKESNRIKPQQVIETLYKLTNGEAYVASDVGQHQM
FAALYYPFDKPRHWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLSKTEKC
>Mature_572_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHLIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVQDPTQIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHDYIYPESIKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISSCEKQILALAEKLNIPVVST
LMGLGAFPGTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNIEKYCPNATILHIDIDPSSISKTVRVDIPI
VGSADIVLDSMLALLEESKESNDSEAINQWWQEITVWRNRNCLAYDKESNRIKPQQVIETLYKLTNGEAYVASDVGQHQM
FAALYYPFDKPRHWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLSKTEKC

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=592, Percent_Identity=27.3648648648649, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI21361361, Length=506, Percent_Identity=24.3083003952569, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=74.1710296684119, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=40.2482269503546, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=33.3333333333333, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=28.75, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=23.3393177737882, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=589, Percent_Identity=24.9575551782683, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=589, Percent_Identity=24.9575551782683, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=607, Percent_Identity=24.5469522240527, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=41.4802065404475, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320816, Length=477, Percent_Identity=25.9958071278826, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=22.5806451612903, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=21.8181818181818, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=496, Percent_Identity=21.7741935483871, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922626, Length=595, Percent_Identity=24.3697478991597, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62746; Mature: 62746

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHLIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVQDPTQIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHDYIYPESI
CCCCHHHHHEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISSCEKQILALAEKLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPGTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNIEKYCPNATI
HHHCCCCCCCCCCCCCEEECCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTVRVDIPIVGSADIVLDSMLALLEESKESNDSEAINQWWQEITVWRNR
EEEECCHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
NCLAYDKESNRIKPQQVIETLYKLTNGEAYVASDVGQHQMFAALYYPFDKPRHWINSGGL
CEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCCCCHHHHCCCCC
GTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLSKTEKC
EEEEECCCCCEEEEEEECCCCCHHHHHHCCCC
>Mature Secondary Structure
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHLIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVQDPTQIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHDYIYPESI
CCCCHHHHHEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISSCEKQILALAEKLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPGTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNIEKYCPNATI
HHHCCCCCCCCCCCCCEEECCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTVRVDIPIVGSADIVLDSMLALLEESKESNDSEAINQWWQEITVWRNR
EEEECCHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
NCLAYDKESNRIKPQQVIETLYKLTNGEAYVASDVGQHQMFAALYYPFDKPRHWINSGGL
CEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCCCCHHHHCCCCC
GTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLSKTEKC
EEEEECCCCCEEEEEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]