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The map label for this gene is pleD [H]

Identifier: 117920670

GI number: 117920670

Start: 2676883

End: 2677911

Strand: Reverse

Name: pleD [H]

Synonym: Shewana3_2227

Alternate gene names: 117920670

Gene position: 2677911-2676883 (Counterclockwise)

Preceding gene: 117920671

Following gene: 117920669

Centisome position: 53.86

GC content: 48.01

Gene sequence:

>1029_bases
ATGTCATCTCCTGCTGCCACTAAAGAGCTCGATTCAGCGGCGCAAATTTTGCGTTTGGCCGTGCCACAAATGTCGGCGCT
CGATATTCCGGTCACCCCAGAGAATTACACGGTTTGGTATGAGTATTTTGCCCAGACGAATCTCGATTTAAATCGCGCTA
TCGATGGCTTTCTGGCGAATAAAGTGGTGTTCACTAAGGAGGTGAATACCAGTCTGTACAAGAATTTTATTCAAGAGAAA
TCCCCCGAAGTCATCGAGAACGTTCAAATCGAAACTCAAATATTGATCAACTCGCTGATCAGTAAAATCACTCAGTTAAA
TCAGGGAACGGAGGCATTTTCAAGCACTCTGGCGGACTTTGGCCTACAGCTTCAGACTAAGCCGGATGTGTGCACGCTCA
ATCAGTTAGTGGATGGGGTGATTGATGAAATGCAAAGTTTACTCGTCAATAACCAAACCATGGAGCAGAGCCTGAGCGCC
ATGGGGCAAGAGGTACAAAACTTAAAGACCGAAATGGAAAATTTAAGTTTGGCGGCGATGACGGACCAATTAACCTCATT
ACATAACCGCCGCGCCTACGAAATTGCGATTCAAGATCATATCCACCGCGCCGAGCAGCAACATACCCGTTGCAGCTTAT
TACTCATTGATATTGACCGCTTTAAAGTGTTTAACGACAACTATGGCCATCAAGTGGGGGATAAGGTGTTAGCCTATGTG
GCGCTCGCCCTCAAACAATGTGTGCGGGGGGATGACTTTGTCGCCCGCTATGGCGGCGAAGAATTTGTGGTGCTGTTGCC
CAATACCCATTTCCACGATGCGTTGCAAGTGGCCGAAACCCTAAGGGAGCGGATTTCTGAGCGGCGCTTAGCCATAGGTA
AAGATAAAAAACTCTCCTTGGGCGCCATCACTGTGTCTATCGGTTTAGCGTCTTTGCAGGAAGGTGATGATGCCGATACC
TTGTTTAGCCGAGCGGATAAAGCCCTGTATGAAGCCAAATCCGATGGGCGCAATTGTGTCCGTGGTTGA

Upstream 100 bases:

>100_bases
TTGCGCCTTATCATAATAATTATTGTGTCCCGTATCCAAATCGAAGCAGTTGAGTTAACTTAAGTTATACAAAAAAGTAA
CAACATTAGGTGCGCGACTC

Downstream 100 bases:

>100_bases
GAGTTACCGCAAAGCGTCAGGTCATACACGCTCTTAACTTATTCCTGTGCCTTTAAGTGCTTTGAGTCAGATAGTCGTTG
GAATCACTCGATGAACAAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSSPAATKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAQTNLDLNRAIDGFLANKVVFTKEVNTSLYKNFIQEK
SPEVIENVQIETQILINSLISKITQLNQGTEAFSSTLADFGLQLQTKPDVCTLNQLVDGVIDEMQSLLVNNQTMEQSLSA
MGQEVQNLKTEMENLSLAAMTDQLTSLHNRRAYEIAIQDHIHRAEQQHTRCSLLLIDIDRFKVFNDNYGHQVGDKVLAYV
ALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERRLAIGKDKKLSLGAITVSIGLASLQEGDDADT
LFSRADKALYEAKSDGRNCVRG

Sequences:

>Translated_342_residues
MSSPAATKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAQTNLDLNRAIDGFLANKVVFTKEVNTSLYKNFIQEK
SPEVIENVQIETQILINSLISKITQLNQGTEAFSSTLADFGLQLQTKPDVCTLNQLVDGVIDEMQSLLVNNQTMEQSLSA
MGQEVQNLKTEMENLSLAAMTDQLTSLHNRRAYEIAIQDHIHRAEQQHTRCSLLLIDIDRFKVFNDNYGHQVGDKVLAYV
ALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERRLAIGKDKKLSLGAITVSIGLASLQEGDDADT
LFSRADKALYEAKSDGRNCVRG
>Mature_341_residues
SSPAATKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAQTNLDLNRAIDGFLANKVVFTKEVNTSLYKNFIQEKS
PEVIENVQIETQILINSLISKITQLNQGTEAFSSTLADFGLQLQTKPDVCTLNQLVDGVIDEMQSLLVNNQTMEQSLSAM
GQEVQNLKTEMENLSLAAMTDQLTSLHNRRAYEIAIQDHIHRAEQQHTRCSLLLIDIDRFKVFNDNYGHQVGDKVLAYVA
LALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERRLAIGKDKKLSLGAITVSIGLASLQEGDDADTL
FSRADKALYEAKSDGRNCVRG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=46.242774566474, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693134, Length=244, Percent_Identity=34.0163934426229, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=37.2972972972973, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787802, Length=203, Percent_Identity=35.9605911330049, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787262, Length=231, Percent_Identity=32.9004329004329, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=30.5084745762712, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=31.8435754189944, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.3170731707317, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=32.9608938547486, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=30.0613496932515, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=30.5882352941176, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=30.6748466257669, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788956, Length=196, Percent_Identity=28.0612244897959, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38186; Mature: 38055

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPAATKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAQTNLDLNRAIDGFLAN
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHC
KVVFTKEVNTSLYKNFIQEKSPEVIENVQIETQILINSLISKITQLNQGTEAFSSTLADF
CEEEEEHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLQLQTKPDVCTLNQLVDGVIDEMQSLLVNNQTMEQSLSAMGQEVQNLKTEMENLSLAAM
CCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLHNRRAYEIAIQDHIHRAEQQHTRCSLLLIDIDRFKVFNDNYGHQVGDKVLAYV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEEEEEEHHHEEEECCCCCHHHHHHHHHHH
ALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERRLAIGKDKKLSL
HHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
GAITVSIGLASLQEGDDADTLFSRADKALYEAKSDGRNCVRG
EEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSPAATKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAQTNLDLNRAIDGFLAN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHC
KVVFTKEVNTSLYKNFIQEKSPEVIENVQIETQILINSLISKITQLNQGTEAFSSTLADF
CEEEEEHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GLQLQTKPDVCTLNQLVDGVIDEMQSLLVNNQTMEQSLSAMGQEVQNLKTEMENLSLAAM
CCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLHNRRAYEIAIQDHIHRAEQQHTRCSLLLIDIDRFKVFNDNYGHQVGDKVLAYV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEEEEEEHHHEEEECCCCCHHHHHHHHHHH
ALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERRLAIGKDKKLSL
HHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
GAITVSIGLASLQEGDDADTLFSRADKALYEAKSDGRNCVRG
EEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA